| |
| Germination Test Methods |
|
Main area
|
|
An important note regarding our germination test methods:
Our methods are dynamic. We may begin with rules listed in standardized germination tests, but we are always trying to improve the accuracy of the results. Consequently, these methods may change over time and in the overwhelming majority of cases will not reflect what are listed in seed germination handbooks or manuals. The germination test methods are provided to let you know how we obtained the percent germination, dormancy, and overall viability as listed on the seed packet sent to you. |
Quick Locators:
Crop Names first letter
A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z
Evaluation Numbers – currently used:
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
? non-Pastinaca
|
|
|
|
|
? Pastinaca
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
|
|
|
|
| |
|
|
|
|
| |
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
TOP
Evaluation Numbers – infrequent or no longer used:
(General, Historic, Special Care)
TOP
|
Amaranth: D. Brenner, Curator
Click here for information on the seed testing papers we use.
Contact Curator David Brenner.
|
ENO: 491828 Amaranth & Cuminum |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: either 4 reps of 50 seed each or 2 reps of 100 seed each for a total of 200 seeds. The first count is done 3 or 4 days after start of test. If needed, second and third counts are done weekly after first count. |
TOP
|
ENO: 493850 Ammi & Orlaya |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
10 ºC |
--- |
|
12 hours |
20 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ºC for 7 days. Transfer to germinator. Temperature at a constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test. |
TOP
|
ENO: 493834 Anethum |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight and then transferred to a germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test. |
TOP
|
ENO: 493921 Angelica |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 1 mM GA3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 10, 17, and 28 days after start of the germination test. |
TOP
|
ENO: 493835 Anthriscus |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test. |
TOP
|
ENO: 493836 Bifora, Dalea, & Galega |
|
Duration |
Temperature |
Fluorescent Lights |
|
10 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight; prechill at 4 ºC for 10 days. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7, 14, and 21 days after start of test. Chip any remaining ungerminated seed at 21 days and then extend test 5 more days. Do final count and end test. |
TOP
|
ENO: 152 Calamovilfa |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 14 days after start of test. |
TOP
|
ENO: 491462 Celosia |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count is done 10 days after start of test. If needed, a second count is done 5 days after first count. |
TOP
|
ENO: 491651 Chenopodium (cultivated) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test. |
TOP
|
ENO: 493487 Chenopodium (dormant/wild species) |
|
Duration |
Temperature |
Fluorescent Lights |
|
1 month |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ºC for one month. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after transfer from cold treatment. |
TOP
|
ENO: 493840 Coniocelinum & Oreoschimperella |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test. |
TOP
|
ENO: 492498 Coriander |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Temperature is constant at 15 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test. |
TOP
|
ENO: 490362 Echinochloa (standard) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test. |
TOP
|
ENO: 491625 Eryngium |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test. |
TOP
|
ENO: 493851 Ferula & Levisticum |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
10 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ºC for 7 days. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test. |
TOP
|
ENO: 493814 Foeniculum |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test. |
TOP
|
ENO: 492586 Melilotus |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
12 hours |
20 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature; prechill at 4 ºC for 14 days. Transfer to germinator. Temperature is constant at 20 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test. |
TOP
|
ENO: 490363 Panicum & Misc. Grasses |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ºC for 7 days. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test. |
TOP
|
ENO: 493899 Perilla |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
20 ºC |
OFF |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between 2 blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 white paper
seeds
(bottom) 1 white paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperature is constant at 20 ºC with dark conditions. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 5 and 14 days after start of test. |
TOP
|
ENO: 493801 Petroselinum |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test. |
TOP
|
ENO: 490361 Setaria (cultivated) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test. |
TOP
|
ENO: 490385 Setaria (dormant/wild species) |
|
Duration |
Temperature |
Fluorescent Lights |
|
2 weeks |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature then place in 4 ºC for 2 weeks. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test. |
TOP
|
ENO: 493852 Sium |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
10 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); make sure blotter paper is very wet. These are kept at room temperature overnight; prechill at 10 ºC for 7 days. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test. |
TOP
|
ENO: 491601 Spinacia |
|
Duration |
Temperature |
Fluorescent Lights |
|
Until seeds sprout |
5 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). The plastic boxes are wrapped with parafilm to help maintain moisture during the long cold treatment. Temperature is a constant 5 ºC until seeds begin to sprout. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur immediately and then 7, 14, 21, and 28 days after transfer from cold treatment. |
TOP
|
ENO: 490972 Umbels
(includes Astrodaucus, Caucalis & Zizia) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test. |
TOP
|
Maize: M. Millard, Curator
|
Click here for information on the seed testing papers we use.
Contact Curator Mark Millard.
|
Zea mays subsp. mays (Maize) |
492358 |
|
Zea (wild forms - teosinte) |
78 |
|
Tripsacum |
491029 |
|
ENO: 492358 Zea mays (subsp. mays) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 °C |
ON |
|
12 hours |
20 °C |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are put in a germinator. Temperatures alternate between 30 °C with light for 12 hours and 20 °C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 7, 10, and 14 days after start of test. Abnormals are not scored until the last count of the test. Because of inbreeding depression in inbred lines, more relaxed parameters are used to classify seedlings as normal. |
TOP
|
ENO: 78 Zea (wild forms - teosinte) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 °C |
ON |
|
12 hours |
20 °C |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 °C with light for 12 hours and 20 °C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur weekly after start of test. |
TOP
|
ENO: 491029 Tripsacum |
|
Duration |
Temperature |
Fluorescent Lights |
|
48 hours |
23 °C |
OFF |
|
12 hours |
25 °C |
ON |
|
12 hours |
23 °C |
OFF |
|
Cupule is nicked and seeds are placed on top of two blue blotter papers treated with 1mM GA3, germinated in a germinator set at 23 °C constant in the dark for 48 hours. They are then rinsed with tap water and placed on new blue blotter papers and place in a germinator with temperatures alternate between 25 °C with light for 12 hours and 23 °C in darkness for 12 hours per 24 hour cycle. Replication and sample size (if quantity is sufficient): 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur as needed. Test is run for up to 6 weeks. |
TOP
|
Oilseeds: L. Marek, Curator
|
Click here for information on the seed testing papers we use.
Contact Curator Laura Marek.
|
ENO: 493335 Alliaria, Erysimum, Hesperis, Sinapis |
|
Duration |
Temperature |
Fluorescent Lights |
| 7 days |
4 ºC |
--- |
| 7 days |
12 hours |
30 ºC |
ON |
| 12 hours |
20 ºC |
OFF |
| 14 days + |
4 ºC |
--- |
| 7 days |
12 hours |
30 ºC |
ON |
| 12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Leave at this regimen for 7 days. Place germination boxes back in cold room for at least 14 days - leave until seeds begin to germinate in cold room. Transfer back to 20/30 ºC, as before. Score immediately and at 7, 14, and 21 days. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. |
TOP
|
ENO: 493339 Alyssum, Berteroa, Crambe,
Eruca, Erucastrum, Isatis
|
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
20 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur weekly after start of test. |
TOP
|
ENO: 491490 Asters |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
16 hours |
25 ºC |
ON |
|
8 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 1 mM GA3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 16 hours and 15 ºC in darkness for 8 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of the germination test.
Additional comments: Madia prefers 15 ºC constant and dark environment. |
TOP
|
ENO: 490360 Biscutella, Linum flavum |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 178 Brassica (carinata, napus, rapa) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature; prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. (Alternatively, 2 reps of 100 seeds.) The first germination count occurs between 3 and 7 days and the last count is on the 14th day after start of test. |
TOP
|
ENO: 181 Brassica (juncea, tournefortii)
|
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
10 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 10 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first germination count occurs between 3 and 7 days and the last count is on the 14th day after start of the germination test. |
TOP
|
ENO: 181 Brassica nigra
|
|
Duration |
Temperature |
Fluorescent Lights |
|
3 days |
10 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 10 ºC for 3 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first germination count occurs between 3 and 7 days and the last count is on the 14th day after start of the germination test. |
TOP
|
ENO: 493043 Brassica repanda |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 493220 Camelina, Goldbachia |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let seeds imbibe overnight at room temperature; prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. One germination count occurs 14 days after start of test; no other counts required. |
TOP
|
ENO: 493034 Cuphea |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit for 48 hours at room temperature. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 2500 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 1500 & < 2500 , then 4 reps of 25 sd for total of 100.
If < 1500, then do not run germination test.
Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 492112 Euphorbia |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight hours at room temperature. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 6 and 16 days after start of test. |
TOP
|
ENO: 105 Helianthus (cultivated) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed in folded paper towels1 moistened with tap water and placed in plastic tubs. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 1500 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 1200 & < 1500 , then 4 reps of 25 sd for total of 100.
If < 1200, then do not run germination test.
Germination counts occur at 5 and 14 days after start of test.
1Seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Towels are kept moist with tap water during the germination period. |
TOP
|
ENO: 131 Helianthus (wilds) |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed in folded paper towels1 moistened with tap water and placed in plastic tubs and left overnight at room temperature. Prechill for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 2000 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 1500 & < 2000 , then 4 reps of 25 sd for total of 100.
If < 1500, then do not run germination test.
Germination counts occur at 5 and 14 days after start of the germination test.
1Seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Towels are kept moist with tap water during the germination period. |
TOP
|
ENO: 492632 Iberis |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") and kept at room temperature for 48 hours. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 493337 Lepidium
|
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed between 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator set at constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur weekly after start of test. |
TOP
|
ENO: 490309 Linum (cultivated) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available:
If > 6000 sds, then 4 reps of 50 sd for total of 200 seeds.
If > 2100 & < 6000 , then 4 reps of 25 sd for total of 100.
If < 2100, then do not run germination test.
Germination count days also vary:
If L. usitatissimum,
then counts occur on 3 and 7 days after start of test.
Otherwise,
counts occur at 7, 10, and 14 days after start of test. |
TOP
|
ENO: 491459 Linum (wilds) |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Transfer to germinator set at constant temperature of 15 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
If seeds do not germinate after 10 days, raise germination temperature to 20 ºC. |
TOP
|
ENO: 493622 Linum lewisii |
|
Duration |
Temperature |
Fluorescent Lights |
|
28 days |
4 ºC |
--- |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ºC for 28 days. Transfer to germinator set at constant temperature of 15 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germiantion test.
If seeds do not germinate after 10 days, raise germination temperature to 20 ºC. |
TOP
|
ENO: 493809 Linum tenuifolium |
|
Duration |
Temperature |
Fluorescent Lights |
|
21 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ºC for 21 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 493336 Matthiola, Thlaspi |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
Ornamentals: M. Widrlechner, Curator
|
Click here for information on the seed testing papers we use.
Contact Curator Mark Widrlechner.
|
ENO: 493243 Actaea |
|
Duration |
Temperature |
Fluorescent Lights |
| 12 hours |
20 ºC |
ON |
| 12 hours |
8 ºC |
OFF |
|
Seeds are soaked in 1 mM GA3 overnight. Rinse seeds with tap water. Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating between 20 ºC with light for 12 hours and 8 ºC in darkness for 12 hours per 24 hour cycle. Leave at this regimen for many days. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts as needed. |
TOP
|
ENO: 493218 Agastache
|
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 8 and 16 days after start of test. |
TOP
|
ENO: 493226 Alcea, Althea |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
20 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let sit overnight at room temperature. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts on 5 and 18 days after start of test. Chip remaining seed. Record final count after 5 more days. |
TOP
|
ENO: 492151 Calendula |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); substrate should be on drier side. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 493795 Centranthus |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of test. |
TOP
|
ENO: 493796 Comarum
|
|
Duration |
Temperature |
Fluorescent Lights |
|
21 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 3 blue blotter (1 blue, 2 white – in contact with seeds) papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 21 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of test. |
TOP
|
ENO: 493228 Dasiphora, Glebionis, Hyssopus
|
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 490482 Dracocephalum, Matricaria |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
20 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 493794 Anthemis, Duchesnea,
Lythrum, Rumex, Sphaeralcea |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of three blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 491056 Echinacea |
|
Duration |
Temperature |
Fluorescent Lights |
|
28 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
25 ºC |
OFF |
|
Soak seeds ovenight in 1 mM Ethephon. Transfer to germination boxes: seeds are placed on top of 2 blue blotter papers moistened with tap water. Prechill at 4 ºC for 28 days. Transfer to germinator with temperature set to a constant 25 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 493957 Heteranthemis |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 493793 Heteropappus
|
|
Duration |
Temperature |
Fluorescent Lights |
|
28 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 28 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 491385 Hyoscymous, Physocarpus |
|
Duration |
Temperature |
Fluorescent Lights |
|
21 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let sit overnight at room temperature. Prechill for 21 days. Remove top blotter paper and transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 493229 Hypericum, Monarda |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
|
ENO: 493958 Lappula |
|
Duration |
Temperature |
Fluorescent Lights |
|
14 days |
4 ºC |
--- |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 14 days. Transfer to germinator with temperature set to a constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 490498 Lavatera, Malva |
|
Duration |
Temperature |
Fluorescent Lights |
|
Constant |
Room Temp |
DARK |
|
Seeds are placed between 2 moist blotter papers and allowed to imbibe water overnight. Seeds are then chipped and placed between blotter papers moistened with tap water in the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
These are kept at room temperature in the dark. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. First count occurs in 4 to 8 days after chipping seed. Germination counts occur weekly after that. |
TOP
|
ENO: 493959 Malope |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let sit overnight at room temperature. Transfer to germinator set at constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 490496 Origanum |
|
Duration |
Temperature |
Fluorescent Lights |
|
Constant |
15 ºC |
--- |
|
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let sit overnight at room temperature. Transfer to germinator with temperature set to a constant 15 ºC. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 493227 Potentilla |
|
Duration |
Temperature |
Fluorescent Lights |
|
21 days |
4 ºC |
--- |
|
7 days |
10 ºC |
OFF |
|
12 hours |
20 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 21 days. Transfer to germinator with temperature set to a constant 10 ºC and darkness for 7 days. Then transfer to germinator with temperature set for 20 C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after transfer to 20 ºC germinator. |
TOP
|
ENO: 493225 Prunella |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
OFF |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed between blotter papers moistened with tap water in the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper
Let sit overnight. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle, no lights. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 490484 Sanvitalia
|
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 493797 Scrophularia
|
|
Duration |
Temperature |
Fluorescent Lights |
|
21 days |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 21 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 490493 Sibiraea, Sorbaria, Spiraea
|
|
Duration |
Temperature |
Fluorescent Lights |
|
28 days |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 28 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 493960 Simsia |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed in folded paper towels1 moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
1Seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Towels are kept moist with tap water during the germination period. |
TOP
|
ENO: 490499 Spergula |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperature set to a constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 492545 Tanacetum
|
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test. |
TOP
|
ENO: 490497 Vaccaria |
|
Duration |
Temperature |
Fluorescent Lights |
|
7 days |
4 ºC |
--- |
|
12 hours |
15 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperature set to a constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
|
Vegetables: K. Reitsma, Curator
|
Click here for information on the seed testing papers we use.
Contact Curator Kathy Reitsma.
|
ENO: 491000 Chicorium |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of two blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 5 and 14 days after start of test. |
TOP
|
ENO: 490384 Cucurbita |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
OFF |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test. |
TOP
|
ENO: 490382 Cucumis |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
OFF |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test. |
TOP
|
ENO: 493996 Daucus |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 7, 14, 21, and (if needed) 28 days after start of test. |
TOP
|
ENO: 490973 Ocimum |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
30 ºC |
ON |
|
12 hours |
20 ºC |
OFF |
|
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7 and 14 after start of test. |
TOP
|
ENO: 490972 Pastinaca |
|
Duration |
Temperature |
Fluorescent Lights |
|
12 hours |
25 ºC |
ON |
|
12 hours |
15 ºC |
OFF |
|
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test. |
TOP
|
Seed Testing Papers
|
|
|
Blotter Paper Types |
|
Blue Blotter Paper |
White Blotter Paper |
|
Composition: |
25% cotton, 75% wood pulp |
75% cotton, 25% wood pulp
with wet strength resin |
|
Basis Weight: |
Listed: 25”x28” / 500 sheets = 250 lb.
= 502 g/m2 |
Listed: 1 lb. / 1389 ft2
= 104.6 g/m2 |
|
Thickness: |
Listed: 31.5 mils
= 80 mm |
Listed: 27.5 mils
= 0.70 mm |
|
Burst Strength:
|
Listed: 45 psi (probably dry)
= 310.3 kPa |
Listed: 200 inches H2O (wet)
= 49.8 kPa |
|
Comparing the blue and white blotter papers when dry, the blue paper was much stronger than the white, but when comparing the blue and white blotter papers when wet, both burst strengths were very similar. |
|
Our usage: |
When seeds are not sensitive to a 'wet' environment. |
When seeds require slightly dryer conditions to promote germination. |
|
Costs: |
lower |
higher |
|
Data source: |
manufacturer |
manufacturer |
|
Manufacturer: |
Anchor Paper |
Ahlstrom Corporation |
|
Disclaimer: |
Mention of trade names or commercial products in this table is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. |
|
Paper Towels used at NCRPIS |
|
Composition: |
100% unbleached sulfate wood pulp |
|
Weight, Color, Texture: |
Regular weight (38 lbs.), tan, creped |
|
Burst strength: |
Listed: 28 psi (probably dry)
= 193.1 kPa |
|
Ash content: |
0.7% maximum |
|
pH of aqueous extract: |
6.5 to 7.5 |
|
Data source: |
manufacturer |
|
Manufacturer: |
Anchor Paper |
|
Disclaimer: |
Mention of trade names or commercial products in this table is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. |
|
General & Historic
|
Evaluation Numbers – infrequent or no longer used:
(General, Historic, Special Care)
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
For additional information click here.
|
|
|
|
|
|
Historic |
ENO: 11 |
Historic |
|
NC7.GERMS.MAIZE.STANDARD |
|
Germinations are performed in a high humidity germinator set at 12 hours 20 degrees centigrade in the dark alternating with 12 hours 30 degrees centigrade in the light. 50 seeds are wrapped in 2 paper towels for each replication. Replications are rubber banded together. 10-20 accessions are placed in plastic tubs with holes in the bottom. Towels are kept moist during germination period. Evaluations are performed at 7 and 10 days. |
TOP
|
Special Care |
ENO: 24 |
Special Care |
|
NC7.GERMS.MAIZE.RESCUE |
|
Seeds are rinsed in 10% bleach and placed on blotters in deep plastic boxes. Separation of seed is 2 cm or more. Fungicides are applied when needed. Boxes are placed in germinator set on alternating 20-30 degrees C and alternating light-dark. When seedling leaves begin emerging from the coleoptile, they are transplanted to 7.6 cm jiffy pots. Soil is added to the pots as the seedlings develop. These jiffy pots are transplanted to large plastic pots when the seedlings are fully established. |
TOP
|
General |
ENO: 150 |
General |
|
NC7.GERMS.C20/30BLOTTERBOX.KNO3 |
|
Seeds are germinated on 2 blotter papers moistened with a 0.1% KN03 solution in plastic boxes (5" x 5-1/4"). Temperatures alternate at 30C with light for 12 hours and 20C in darkness for 12 hours per 24 hour cycle. |
TOP
|
Historic |
ENO: 180 |
Historic |
|
NC7.GERMS.BRASSICA.KNO3.ROOM |
|
Germinations are performed at room temperature (~20 degrees C) with 0.2% percent potassium nitrate as the medium. Fifty seeds are put on top of blotters for each replication. The number of germinated, abnormal, and hard seeds are counted at 3 and 7 days after placing on blotters. |
TOP
|
General |
ENO: 367 |
General |
|
NC7.GERMS.C10BLOTTER.BOX |
|
Seeds are germinated on blotters moistened with 0.2% KNO3 water in plastic boxes. The temperature is 10 deg C, the daylength is 12 to 14 hours, for 21 days. |
TOP
|
Special Care |
ENO: 399 |
Special Care |
|
NC7.GERMS.CUPHEA.EXCISED |
|
Seeds are soaked in water overnight or a minimum of four hours. Seed coats are removed. Seeds are placed on two blotters in a germ box. Keep blotters soaked with excess water at all times. Place under 12 hours of light at room temperature. Count germs every seven days for 21 days. |
TOP
|
Historic |
ENO: 400 |
Historic |
|
NC7.GERMS.CUPHEA.NONEXCISED |
|
Seeds are placed on two blotter pads in a germ box. Blotter pads are kept soaked with excess water at all times. Germ box is placed in 12 hours of light at room temperature. Germs are counted every seven days for 21 days. |
TOP
|
Historic |
ENO: 404 |
Historic |
|
NC7.GERMS.CRUCIFERS.STANDARD |
|
Four replicates of fifty seeds are each folded into a double layer of germ toweling moistened with water and placed in a lighted germination chamber ( 16/8 ) at 20 degrees C. Evaluations are performed at 4 and 7 days following I.S.T.A. and A.O.S.A. rules. An optional prechill at 5-10 degrees C for up to 7 days may be used to hasten and unify germination and may be used as test environment.
Additional comments: Crambe: 0.1% potassium nitrate solution is used to initially moisten toweling. |
TOP
|
Historic |
ENO: 490012 |
Historic |
|
NC7.GERMS.MAIZE.ISU.COLD |
|
Performed by ISU Seed Lab. Stress condition. |
TOP
|
Historic |
ENO: 490013 |
Historic |
|
NC7.GERMS.MAIZE.ISU.WARM |
|
Performed by ISU Seed Lab. |
TOP
|
Historic |
ENO: 490383 |
Historic |
|
NC7.GERMS.DAUCUS.STANDARD |
|
Seeds are placed on top of 2 blue blotter papers and one sheet of pleated paper moistened with tap water (substrate on dry side) in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 7, 21, and 28 days after start of test. |
TOP
|
Historic |
ENO: 490483 |
Historic |
|
NC7.GERMS.HORT.KPAC.1WKPRECHILL.20/30 |
|
No method described except in evaluation name. |
TOP
|
Historic |
ENO: 490487 |
Historic |
|
NC7.GERMS.HORT.TOWELS.DARK.20C |
|
No method described except in evaluation name. |
TOP
|
General |
ENO: 490500 |
General |
|
NC7.GERMS.20C.CONSTANT |
|
Seeds are imbibed with tap water on 2 germination papers in a plastic box (5" x 5-1/4"). Temperature and light regimen is 20 C with fluorescent light per 12 hours per 24 hour cycle, alternating with 20 C in darkness. |
TOP
|
Historic |
ENO: 490501 |
Historic |
|
NC7.GERMS.HORT.1.WEEK.PRECHILL.20C |
|
No method described except in evaluation name. |
TOP
|
Historic |
ENO: 490502 |
Historic |
|
NC7.GERMS.HORT.KPAC.KNO3.20/30C |
|
No method described except in evaluation name. |
TOP
|
Historic |
ENO: 490506 |
Historic |
|
NC7.GERMS.HORT.KNO3.15C.1WKPRECHILL |
|
No method described except in evaluation name. |
TOP
|
Special Care |
ENO: 490639 |
Special Care |
|
NC7.GERMS.MAIZE.MILD.HYDRATION |
|
Seeds are prehumidified 24 hours by placing seeds in accelerated aging tray with seeds above distilled water. Temperature is held at a constant 22C. Lights are on 0800-2200 and off 2200-0800. Seeds are then transferred to a double layer of germination blotter paper with 20 ml of distilled water. From 0800-2200 temperature is 22C and lights are on. From 2200-0800 temperature is 20C and lights are off. Counts are made at 3 day intervals until completion. Note: Accelerated aging trays are transparent polystyrene containers (4 5/16" x 4 5/16" x 1 3/8") that have a wire mesh screen that keeps seeds approximately 5/8" above the bottom of the container. The bottom of the container has a small amount of water added to achieve the high humidity atmosphere. |
TOP
|
Special Care |
ENO: 490674 |
Special Care |
|
NC7.GERMS.MAIZE.LOW.AMOUNT |
|
This method is the same as NC7.GERMS.MAIZE.STANDARD except the quantity and replications are different because there is a low quantity of seed to germinate. The recommended quantity for this method is 4 replications of 10 seeds each. Germinations are performed in a high humidity germinator set at 12 hours 20 degrees centigrade in the dark alternating with 12 hours 30 degrees centigrade in the light. Seeds are wrapped in 2 paper towels for each replication. Replications are rubber banded together. Towels are kept moist during germination period. |
TOP
|
Historic |
ENO: 490995 |
Historic |
|
NC7.GERMS.ASPARAGUS.STANDARD |
|
4 Reps 50 ct ea.; Towels, 8/16 hours lights at 20/30 C.
Asparagus is now curated at NE9, Geneva, NY. |
TOP
|
Special Care |
ENO: 491028 |
Special Care |
|
NC7.GERMS.MAIZE.TRIPSACUM_LONGCOLD |
|
Seeds soaked in a .25% Thiram solution for 10-12 hours, drained and stored in the dark at 35-45 F (~4 C) for 6-8 weeks. They are then placed in a 25/23 germinator with lights 12/12 on/off. |
TOP
|
General |
ENO: 491156 |
General |
|
NC7.GERMS.25C_CONSTANT.LIGHT_12H |
|
Seeds are imbibed with tap water on 2 germination papers in a plastic box (5" x 5-1/4"). Temperature is held constant at 25 C with 12 hours of fluorescent light per 24 hour cycle. |
TOP
|
Historic |
ENO: 491200 |
Historic |
|
NC7.GERMS.HORT.60DAYPRECHILL.20/30 |
|
No method described except in evaluation name. |
TOP
|
Historic |
ENO: 491297 |
Historic |
|
NC7.GERMS.LINUM.ND.HISTORICAL |
|
Germination data received from Fargo, ND when crop was transferred. Germination percentage was determined from 100 seeds placed in a 9 cm diameter petri dish on a filter paper wetted with distilled water. The number of germinated seeds were recorded after 72 hours. |
TOP
|
General |
ENO: 491369 |
General |
|
NC7.GERMS.ALL.PT1KNO3.30C14HR.20C10HR |
|
Germinations were conducted utilizing 5" x 5 1/4" x 1 1/4" germination boxes with 2 germination papers in each box. Germ papers were initially wetted with 0.1% KNO3 distilled water solution and the seeds were then evenly distributed on the germ paper. Germ boxes were then placed into germination chambers set at 30 C with fluorescent lights for 14 hrs alternating with 20 C and dark for 10 hrs. Follow up wettings of the germination papers were with distilled water. [Note: 0.1% KNO3 was used to alleviate negative effect of higher concentrations sometimes prescribed. 14 hr / 10 hr cycles were used to more closely simulate temperate zone conditions.] |
TOP
|
Historic |
ENO: 491386 |
Historic |
|
NC7.GERMS.HORT.KNO3.20/30 |
|
No method described except in evaluation name. |
TOP
|
Historic |
ENO: 491460 |
Historic |
|
NC7.GERMS.FENNEL |
|
Two replications of 100 seeds each; Towels; Pre-chill ( 4C ) for 7 days; 20/30 dark; 7 and 14 days. Then, light ( 16/8h ); and counts at 21 and 28 days. May continue to germinate up to 6 weeks. |
TOP
|
Occasionally used |
ENO: 491489 |
Occasionally used |
|
NC7.GERMS.CUCUMIS.WILDS |
|
Towels; H20; Pre-chill (7-14d@4C); 10/30C; 7,14 and 21days. |
TOP
|
General |
ENO: 491579 |
General |
|
NC7.GERMS.15/25C.LIGHT_12H |
|
Seeds are imbibed with tap water on 2 germination papers in a plastic box (5" x 5-1/4"). Temperature and light regimen is 25 C with fluorescent light per 12 hours per 24 hour cycle, alternating with 15 C in darkness. |
TOP
|
General |
ENO: 491609 |
General |
|
NC7.GERMS.5DEG.C |
|
Two replications of 100 seeds each; Blotter (TP) moist with 0.1%KNO3; keep in cooler 5 deg. centigrade, counts 7, 14, 21 and 28 days. |
TOP
|
Historic |
ENO: 491624 |
Historic |
|
NC7.GERMS.OCIMUM.DORMANCY |
|
Towels; 0.1% KNO3; 16/8 light; 20/30C; 7 and 14 days. If no response after 10 days high temp. is moved up to 32C. *With proper alternating temperature regimen light is not an absolute requirement. (Method from previous technician; moved from standard method, which was set back to original standard eval description.) |
TOP
|
Historic |
ENO: 491849 |
Historic |
|
NC7.GERMS.HORT.15MINUTESH2SO4.TOWEL.DARK |
|
No method described except in evaluation name. |
TOP
|
General |
ENO: 491857 |
General |
|
NC7.GERMS.MAIZE.ROOM |
|
Germinations are performed at room tempurature close to 25 C. 50 seeds are wrapped in 2 paper towels for each replication. Replications are rubber banded together. 10-20 accessions are placed in plastic tubs without holes in the bottom. Towels are kept moist during germination period. Evaluations are performed at 7 and 10 days. |
TOP
|
Historic |
ENO: 491878 |
Historic |
|
NC7.GERMS.UMBELS.DORM.TAP |
|
Two replications of 100 seeds each, or 4 replications of 50 seeds each; Blotter (TP); Pre-chill (4C) 7 days; 20/30 dark with counts 7 and 14 days; then 16/8hrs lights, 20/30 C with counts 7, 14, 21 and 28 days. Additional comments: The initial dark environment can be ignored for those species with a known light requirement; which are numerous in this family. Chaerophyllum and Eryngium can take up to two months to fully germinate (Chaerophyllum will not usually germinate until at least 4 weeks). (Method from previous technician; moved from standard method, which was set back to original standard eval description.) Use only tap water (changed from KNO3 in 2002) add tap water when dry. Use the 20/30 germinator with light/dark in most cases. In cases where two seeds are attached together, esp. in Coriandrum, the seeds should be separated, seeds that crumble (because they are undeveloped) are not counted and are excluded from the germination sample of 200 non-crumbling seeds. |
TOP
|
Historic |
ENO: 492108 |
Historic |
|
NC7.GERMS.CRAMBE |
|
BLOTTERS, KNO3 AT 20C. COUNTS ON DAYS 4 AND 7. |
TOP
|
Historic |
ENO: 492293 |
Historic |
|
NC7.GERMS.CORIANDRUM |
|
4 Reps of 50 seed/rep. Total of 200 seed. 2 sheets of paper toweling wetted with water. Constant 15C germinator with 12h light/12h dark. Counts on day 6 and 21. |
TOP
|
Occasionally used |
ENO: 492308 |
Occasionally used |
|
NC7.GERMS.CORONILLA./.SECURIGERA |
|
BLOTTER BOXES WITH WATER. EXCESSIVE MOISTURE(VERY WET). 20C CONSTANT WITH 12hr DARK/12hr LIGHT. 1ST COUNT DAY 7 2ND AND FINAL COUNT DAY 14. |
TOP
|
Historic |
ENO: 492423 |
Historic |
|
NC7.GERMS.MELILOTUS |
|
TAP WATER, BLOTTER BOXES, 4 REPS, 50 SEEDS/REP. 20C CONSTANT WITH 12d/12L |
TOP
|
Special Care |
ENO: 492426 |
Special Care |
|
NC7.GERMS.CHORISPORA |
|
0.1% KNO3 2 BLOTTERS IN BLOTTER BOXES. 200 SEED, 50 SEED/REP. SCARIFY SEED WITH 150 GRIT SANDPAPER ON RUBBING BOARD. 20C CONSTANT 24H DARK. |
TOP
|
Historic |
ENO: 492450 |
Historic |
|
NC7.GERMS.SETARIA.15/25 |
|
Blotter boxes with water. Germinator is set at 15/25 12 hours dark/12 hours light. Counts on day 7 and day 21. |
TOP
|
Special Care |
ENO: 492558 |
Special Care |
|
NC7.GERMS.HORT.CHIP.BLOTTER.20C.DARK |
|
200 SEED TOTAL. 4 REPS, 50 SEED/REP. ALL SEEDS ARE CHIPPED WITH A STRAIGHT EDGE RAZOR BLADE. SEEDS ARE THEN PLACED IN A BLOTTER BOX WITH 2 BLUE BLOTTERS AND ONE WHITE BLOTTER MOISTENED WITH WATER. BOXES WERE COVERED WITH A BLACK TRASH BAG AND PLACED IN A CABINET. COUNTS ON DAY 7,14 AND 21. |
TOP
|
Historic |
ENO: 492566 |
Historic |
|
NC7.GERMS.FLAX.WILDS.2005 |
|
Use two full size germination papers for each germ box. Add 25ml of 3 mM GA3 solution (= 1.038 gm/l or approx. 1 gm GA3 / liter DI water). Put 50 wild flax seeds on germ paper. Put in germination chamber set for: Temperature: 23 C (12 hrs) / 9 C (12 hrs). That is, from 8:00 am to 8:00 pm. Lights: On (11.5 hrs) / Off (12.5 hrs). That is, from 8:30 am to 8:00 pm. GA3 treatment should last 48 hrs. Then, transfer seeds to new germ paper wetted with tap or DI water. Keep germ boxes in the 23/9 C regimen as described above for entire germ test. Recommend counts be taken at 10 da, 14 da, and 21 da. |
TOP
|
General |
ENO: 492572 |
General |
|
NC7.GERMS.TZ |
|
Tetrazolium Test. |
TOP
|
Special Care |
ENO: 492614 |
Special Care |
|
NC7.GERMS.ULMUS.EXCISED |
|
ALL SEED IS PLACED BETWEEN TWO MOISTENED BLOTTER PAPERS IN THE DARK, AT ROOM TEMP. FOR 2 TO 3 DAYS. SEEDS THAT DO NOT HAVE RADICLE PROTRUSION AFTER CONDITIONING TREATMENT ARE THEN EXCISED FROM THEIR PERICARP AND SEEDCOAT. SEEDS THAT HAVE RADICLE PROTRUSION ARE NOT EXCISED. ALL SEEDS ARE THEN PLACED ON BLOTTER PAPER AND PLACED IN A 20/30 GERMINATOR WITH 12d/12L UNTIL READY TO COUNT. |
TOP
|
Special Care |
ENO: 492640 |
Special Care |
|
NC7.GERMS.ULMUS.NONEXCISED |
|
200 SEEDS. 4 REPS WITH 50 SEED/REP. BLOTTER BOXES WITH WATER. SET AT ROOM TEMPERATURE OVERNIGHT AND THEN PRECHILL FOR 3 MONTHS AT 4C. TRANSFER TO A 20/30 GERMINATOR WITH 12D/12L. COUNTS ON DAY 7, 14 AND 21. |
TOP
|
General |
ENO: 492969 |
General |
|
NC7.GERMS.HORT.90DAYPRECHILL.20/30 |
|
Blotter Boxes. 2 blue blotters and 1 white. Tap water. Place in cold room for 90 days and then transfer to a 20/30 germinator with 12hr. dark/12hr light. |
TOP
|
Historic |
ENO: 493010 |
Historic |
|
NC7.GERMS.HORT.KNO3.15C |
|
No method described except in evaluation name. |
TOP
|
Historic |
ENO: 493018 |
Historic |
|
NC7.GERMS.CRUCIFERS.ERUCA |
|
Four replicates of fifty seeds are each placed onto a double layer of blue germ paper and a single layer of white germ blotters, moistened with water and placed in a lighted germination chamber ( 12/12 ) at 20 degrees C. Evaluations are performed at 7 days only. An optional prechill at 5-10 degrees C for up to 7 days may be used to hasten and unify germination and may be used as test environment. |
TOP
|
General |
ENO: 493044 |
General |
|
NC7.GERMS.5.DAY.COLD.0.1%KNO3BLOTTER.15C |
|
200 SEED, 4 REPS, 50SEED/REP. BLOTTER BOXES. 0.1% KNO3. 5 DAY PRECHILL. 15C CONSTANT. 12D/12L. |
TOP
|
Historic |
ENO: 493219 |
Historic |
|
NC7.GERMS.MONARDA |
|
KNO3, TOP OF BLOTTERS LAYERED 1 BLUE, 1 WHITE, 1 BLUE. 20C/30C, 12D/12L. COUNTS DAY 7 + 28. |
TOP
|
Historic |
ENO: 493222 |
Historic |
|
NC7.GERMS.SETARIA.15/30 |
|
BLOTTER BOXES, INBETWEEN BLOTTERS, BLUE, WHITE, SEEDS, BLUE ON TOP. H20. 15C/30C COUNTS ON DAYS 4 AND 10. |
TOP
|
Historic |
ENO: 493375 |
Historic |
|
NC7.GERMS.HYPERICUM.MCCOY |
|
PETRI PLATES, DISTILLED H20, CUT BLOTTERS INTO SMALL SQUARES PLACE 2 BLUE AND 1 WHITE. PLACE SEEDS ON TOP OF WHITE BLOTTER AND PLACE IN 8/20 CELCIUS GERMINATOR 12D/12L. CHECK FOR RADICLE EMERGENCE IN 7 DAYS. |
TOP
|
Occasionally used |
ENO: 493525 |
Occasionally used |
|
NC7.GERMS.CICUTA |
|
BLOTTER BOXES. 1BLUE BLOTTER, 1WHITE, 1 BLUE ON TOP. VERY WET BLOTTERS. SEEDS ON TOP OF BLOTTERS. SPRAY LIGHTLY WITH MORE WATER AND SET AT ROOM TEMPERATURE OVERNIGHT. 2 WEEK PRECHILL. PLACE IN GERMINATOR 7C/15C, 12/12 LIGHTING. |
TOP
|
Special Care |
ENO: 493603 |
Special Care |
|
NC7.GERMS.X-RAY |
|
Samples were x-rayed. Seeds that were filled are considered normal and empty seeds are considered abnormal. |
TOP
|
|
|
|
|
|
Last Modified: 02/13/2009
|
|
