Bibliographic Citation
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Title | Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: Identification of alcohol dehydrogenase II as a stress protein |
Creator/Author | Haejung An ; Keshav, K.F. ; Ingram, L.O. (Univ. of Florida, Gainesville (United States)) ; Scopes, R.K. ; Rodriguez, M. (La Trobe Univ., Victoria (Australia)) |
Publication Date | 1991 Oct 01 |
OSTI Identifier | OSTI ID: 5865549 |
Other Number(s) | ISSN0021-9193; CODEN: JOBAA |
Resource Type | Journal Article |
Resource Relation | Journal of Bacteriology ; Vol/Issue: 173:19; DOE Project |
Subject | 550200 -- Biochemistry ;560300 -- Chemicals Metabolism & Toxicology ;560200 -- Thermal Effects; ALCOHOL DEHYDROGENASE-- BIOCHEMICAL REACTION KINETICS;ZYMOMONAS MOBILIS-- ENZYME ACTIVITY; BIOLOGICAL PATHWAYS;BIOLOGICAL STRESS;ETHANOL;GENES;TEMPERATURE EFFECTS |
Related Subject | ALCOHOLS;BACTERIA;ENZYMES;HEMIACETAL DEHYDROGENASES;HYDROXY COMPOUNDS;KINETICS;MICROORGANISMS;ORGANIC COMPOUNDS;OXIDOREDUCTASES;PROTEINS;REACTION KINETICS |
Description/Abstract | The 13 major enzymes which compose the glycolytic and fermentative pathways in Zymomonas mobilis are particularly abundant and represent one-half of the soluble protein in exponential-phase cells.^One- and two-dimensional polyacrylamide gel electrophoresis maps were developed for 12 of these enzymes.^Assignments were made by comigration with purified proteins, comparison with overexpressed genes in recombinant strains, and Western blots (immunoblots).^Although most glycolytic enzymes appeared resistant to turnover and accumulated in stationary-phase cells, the protein levels of pyruvate kinase, alcohol dehydrogenase I, and glucokinase declined.^Alcohol dehydrogenase II was identified as a major stress protein and was induced both by exposure to ethanol and by elevated temperature (45C).^This enzyme, encoded by the adhB gene, is expressed from tandem promoters which share partial sequence identity with Escherichia coli consensus sequence for heat shock proteins. |
Country of Publication | United States |
Language | English |
Format | Pages: 5975-5982 |
System Entry Date | 2001 May 13 |
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