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Title Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: Identification of alcohol dehydrogenase II as a stress protein
Creator/Author Haejung An ; Keshav, K.F. ; Ingram, L.O. (Univ. of Florida, Gainesville (United States)) ; Scopes, R.K. ; Rodriguez, M. (La Trobe Univ., Victoria (Australia))
Publication Date1991 Oct 01
OSTI IdentifierOSTI ID: 5865549
Other Number(s)ISSN0021-9193; CODEN: JOBAA
Resource TypeJournal Article
Resource RelationJournal of Bacteriology ; Vol/Issue: 173:19; DOE Project
Subject550200 -- Biochemistry ;560300 -- Chemicals Metabolism & Toxicology ;560200 -- Thermal Effects; ALCOHOL DEHYDROGENASE-- BIOCHEMICAL REACTION KINETICS;ZYMOMONAS MOBILIS-- ENZYME ACTIVITY; BIOLOGICAL PATHWAYS;BIOLOGICAL STRESS;ETHANOL;GENES;TEMPERATURE EFFECTS
Related SubjectALCOHOLS;BACTERIA;ENZYMES;HEMIACETAL DEHYDROGENASES;HYDROXY COMPOUNDS;KINETICS;MICROORGANISMS;ORGANIC COMPOUNDS;OXIDOREDUCTASES;PROTEINS;REACTION KINETICS
Description/Abstract The 13 major enzymes which compose the glycolytic and fermentative pathways in Zymomonas mobilis are particularly abundant and represent one-half of the soluble protein in exponential-phase cells.^One- and two-dimensional polyacrylamide gel electrophoresis maps were developed for 12 of these enzymes.^Assignments were made by comigration with purified proteins, comparison with overexpressed genes in recombinant strains, and Western blots (immunoblots).^Although most glycolytic enzymes appeared resistant to turnover and accumulated in stationary-phase cells, the protein levels of pyruvate kinase, alcohol dehydrogenase I, and glucokinase declined.^Alcohol dehydrogenase II was identified as a major stress protein and was induced both by exposure to ethanol and by elevated temperature (45C).^This enzyme, encoded by the adhB gene, is expressed from tandem promoters which share partial sequence identity with Escherichia coli consensus sequence for heat shock proteins.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 5975-5982
System Entry Date2001 May 13

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