CRIS NUMBER: 0177541
SUBFILE: CRIS
PROJECT NUMBER: CALV-HOPKINS-96-12
SPONSOR AGENCY: CSVM
PROJECT TYPE: STATE
PROJECT STATUS: TERMINATED
MULTI-STATE PROJECT NUMBER: (N/A)
START DATE: Aug 1, 1996
TERMINATION DATE: Sep 30, 2005
GRANT PROGRAM: (N/A)
GRANT PROGRAM AREA: (N/A)
CLASSIFICATION
722 | 6010 | 1130 | 4.2 | 50% |
722 | 6010 | 1090 | 4.2 | 50% |
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CLASSIFICATION HEADINGS
KA722 - Zoonotic Diseases and Parasites Affecting Humans S6010 - Individuals F1130 - Entomology and acarology F1090 - Immunology G4.2 - Reduce Number and Severity of Pest and Disease Outbreaks
RESEARCH EFFORT CATEGORIES
BASIC |
100% |
APPLIED |
(N/A)% |
DEVELOPMENTAL |
(N/A)% |
KEYWORDS: human diseases; bacterial diseases; ixodidae; disease vectors; ehrlichia equi; granulocytes; serology; ehrlichiosis; disease prevalence; zoonoses; antibodies; serological tests; polymerase chain reaction; disease detection
PROGRESS: Aug 1, 1996 TO Sep 30, 2005
Following intravenous inoculation with horse blood-infected with the agent of human granulocytic ehrlichiosis (HGE) from a human fatality, two rhesus macaques (Macaca mulatta) exhibited pyrexia and lethargy on days 4-12 postinfection (PI). Hematology revealed neutropenia, thrombocytopenia, and anemia, with ehrlichial morulae in monocytes and neutrophils on days 4-12. Blood was polymerase chain reaction (PCR)-positive on days 4-12 and bone marrow was PCR-positive on day 11. There was a minor increase in gamma-glutamyl transpeptidase on day 12 and serum interferon-gamma levels increased by day 18. Seroconversion occurred on day 20 PI to a titer of 100 by day 22. Western blot bands characteristic of HGE included 25-, 44-, 80-, 94-, 105-, and 125-kD bands. There was generalized lymphohistiocytic infiltration in the liver, spleen, lymph nodes, and other tissues. The liver had focal hepatocyte apoptosis. There was HGE DNA (by PCR) only in the spleen. Comparable findings were
not observed in a monkey that received uninfected horse blood as a control.
IMPACT: 1996-08-01 TO 2005-09-30
This animal model of human disease is important for further studies of HGE diagnosis, management, and pathogenesis.
PUBLICATION INFORMATION: 1996-08-01 TO 2005-09-30
Foley JE, Lerche NW, Dumler JS, Madigan JE. 1999. A simian model of human granulocytic ehrlichiosis. Am J Trop Med Hyg 1999 Jun;60(6):987-93
PROJECT CONTACT INFORMATION
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