PROW: CD66d

PROW and IWHLDA present the GUIDE on:
CD66d
Authors: Keith Skubitz; Fritz Grunert; Peter Jantscheff; Motomu Kuroki; Amy Skubitz
Reviewer:
Diverse coauthors in lieu of reviewer
Link to additional info in FORUM [GUIDE BAR]

ALTERNATE NAMES FOR CD66d

CGM1

MAJOR LINKS FOR CD66d

NCBI LocusLink Record: 1084
SwissProt annotated protein record: P40198

FUNCTION BIOCHEMICAL ACTIVITY OF CD66d - Unclear, see ligands

CELLULAR FUNCTION OF CD66d

Unclear
Capable of activating neutrophils and functions as a receptor for Neisseria gonorrhea and N. meningitidis

DISEASE RELEVANCE OF CD66d AND FUNCTION OF CD66d IN INTACT ANIMAL

Unclear, see cellular function

STRUCTURE MOLECULAR FAMILY FOR CD66d

Families in which CD66d is a member
- CD66d-->carcinoembryonic antigen-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD66d

Leader 34aa, extracellular 108aa, TM 39aa, cytoplasmic 71aa
2 YXXL motifs in cytoplasmic tail
1 extracellular Ig-V like domain

MOLECULAR MASS OF CD66d

CELL TYPE	MW UNREDUCED	MW REDUCED	Comment
Granulocytes		35 kDa

POST-TRANSCRIPTIONAL MODIFICATION OF CD66d

There are 3 splice variants, one of which alters the reading frame leading to the loss of YXXL motifs

POST-TRANSLATIONAL MODIFICATION OF CD66d

Heavily and variably N-glycosylated; (2 N-glycosylation sites), two potential phosphorylation sites in cytoplasmic domain

MOLECULAR INTERACTIONS PROTEINS AND DNA ELEMENTS WHICH REGULATE TRANSCRIPTION OF CD66d - No information

SUBSTRATES FOR CD66d - No information

ENZYMES WHICH MODIFY CD66d - No information

LIGANDS FOR CD66d AND MOLECULES ASSOCIATED WITH CD66d Link to additional info in FORUM

MOLECULE	COMMENT
None known	No homotypic or heterotypic adhesion has been identified

EXPRESSION MAIN CELLULAR EXPRESSION OF CD66d - Granulocytes

AUTHOR'S ADDITIONAL INSIGHTS ON CD66d

CD66d may play a signaling role and regulates the adhesion activity of CD11/CD18 in neutrophils

REAGENTS CD66d-SPECIFIC MABS NEWLY ASSIGNED AT SIXTH INTERNATIONAL WORKSHOP - See reference mAb

SELECTION OF OTHER CD66d-SPECIFIC REFERENCE MAB

NAME(Workshop IDs)	SOURCE or REFERENCE	COMMENT
COL-1	Dr. Jeffrey Schlom, Laboratory of Tumor Immunology and Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892

SELECTED REFERENCES ON CD66d

REVIEWS

PRIMARY CITATIONS

1. Thompson JA,Grunert F,Zimmermann W Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal 1991 5:344 PubMed

2. Kuroki M,Arakawa F,Matsuo Y,Oikawa S,Misumi Y,Nakazato H,Matsuoka Y Molecular cloning of nonspecific cross-reacting antigens in human granulocytes. J Biol Chem 1991 266:11810 PubMed

3. Nagel G,Grunert F,Kuijpers TW,Watt SM,Thompson J,Zimmermann W Genomic organization, splice variants and expression of CGM1, a CD66- related member of the carcinoembryonic antigen gene family. Eur J Biochem 1993 214:27 PubMed

4. Skubitz KM,Campbell KD,Ahmed K,Skubitz AP CD66 family members are associated with tyrosine kinase activity in human neutrophils. J Immunol 1995 155:5382 PubMed

5. Skubitz KM,Campbell KD,Skubitz AP CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J Leukoc Biol 1996 60:106 PubMed

6. Chen T,Gotschlich EC CGM1a antigen of neutrophils, a receptor of gonococcal opacity proteins. Proc Natl Acad Sci U S A 1996 93:14851 PubMed

WWW RESOURCES

* indicates ammended by reviewer, ** indicates added by reviewer

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99 mpr@mail.nih.gov