• CD67
• CGM6
• NCA-95

MAJOR LINKS FOR CD66b  

• NCBI LocusLink Record: 1088
• Mendelian Inheritance in Man (OMIM): 163980
• SwissProt annotated protein record: P31997

CELLULAR FUNCTION OF CD66b  

• Unclear, but capable of heterophilic adhesion, and transmembrane signaling
• Capable of activating neutrophils

DISEASE RELEVANCE OF CD66b AND FUNCTION OF CD66b IN INTACT ANIMAL  

• Unclear, see cellular function

• Families in which CD66b is a member
  • CD66b-->carcinoembryonic antigen-->immunoglobulin supergene family

MOLECULAR STRUCTURE OF CD66b  

• Leader 34aa, extracellular 284aa, a 29aa hydrophobic domain replaced with GPI
• 2 Ig C2 domains and 1 IgG-V like domain

MOLECULAR MASS OF CD66b  

POST-TRANSCRIPTIONAL MODIFICATION OF CD66b  

• GPI-linked, no splice variants

POST-TRANSLATIONAL MODIFICATION OF CD66b  

• Heavily and variably N-glycosylated; (11 N-glycosylation sites)

SUBSTRATES FOR CD66b   - No information

ENZYMES WHICH MODIFY CD66b   - No information

LIGANDS FOR CD66b AND MOLECULES ASSOCIATED WITH CD66b  

• CD66b exhibits only heterotypic adhesion with CD66c with no homophilic activity, suggesting the possibility that CD66b plays a role in the interaction between granulocytes or between granulocytes and epithelial cells
• The data are consistent with the hypothesis that CD66b plays a signaling role and regulates the adhesion activity of CD11/CD18 in neutrophils. While the details of the "activation signal" transmitted by CD66 antigens are not known, the finding of tyrosine kinase activity associated with CD66a, CD66b, and CD66c suggests that these kinase activities may be involved in signal transduction via CD66 family members
• CD66b is released during granulocyte activation, leading in vitro and in vivo to soluble protein with unknown function

SELECTION OF OTHER CD66b-SPECIFIC REFERENCE MAB  

REVIEWS

PRIMARY CITATIONS

1. Buchegger F,Schreyer M,Carrel S,Mach JP Monoclonal antibodies identify a CEA crossreacting antigen of 95 kD Int J Cancer 1984 33:643 PubMed

2. Arakawa F,Kuroki M,Misumi Y,Oikawa S,Nakazato H,Matsuoka Y Characterization of a cDNA clone encoding a new species of the nonspecific cross-reacting antigen (NCA), a member of the CEA gene family. Biochem Biophys Res Commun 1990 166:1063 PubMed

3. Berling B,Kolbinger F,Grunert F,Thompson JA,Brombacher F,Buchegger F,von Kleist S,Zimmermann W Cloning of a carcinoembryonic antigen gene family member expressed in leukocytes of chronic myeloid leukemia patients and bone marrow. Cancer Res 1990 50:6534 PubMed

4. Oikawa S,Inuzuka C,Kuroki M,Arakawa F,Matsuoka Y,Kosaki G,Nakazato H A specific heterotypic cell adhesion activity between members of carcinoembryonic antigen family, W272 and NCA, is mediated by N-domains. J Biol Chem 1991 266:7995 PubMed

5. Lund-Johansen F,Olweus J,Symington FW,Arli A,Thompson JS,Vilella R,Skubitz K,Horejsi V Activation of human monocytes and granulocytes by monoclonal antibodies to glycosylphosphatidylinositol-anchored antigens. Eur J Immunol 1993 23:2782 PubMed

6. Kuijpers TW,van der Schoot CE,Hoogerwerf M,Roos D Cross-linking of the carcinoembryonic antigen-like glycoproteins CD66 and CD67 induces neutrophil aggregation. J Immunol 1993 151:4934 PubMed

7. Skubitz KM,Campbell KD,Ahmed K,Skubitz AP CD66 family members are associated with tyrosine kinase activity in human neutrophils. J Immunol 1995 155:5382 PubMed

8. Skubitz KM,Campbell KD,Skubitz AP CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J Leukoc Biol 1996 60:106 PubMed

9. Thompson JA,Grunert F,Zimmermann W Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal 1991 5:344 PubMed

WWW RESOURCES

Portions copyright by Garland Press and by the International Workshops on Human Leukocyte Differentiation Antigens; used with permission

Modified 10/14/99   mpr@mail.nih.gov