Bibliographic Citation
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Title | Virions and intracellular nucleocapsids produced during mixed heterotypic influenza infection of MDCK cells |
Creator/Author | Sklyanskaya, E.I. ; Varich, N.L. ; Amvrosieva, T.V. ; Kaverin, N.V. |
Publication Date | 1985 Feb 01 |
OSTI Identifier | OSTI ID: 5356234 |
Other Number(s) | CODEN: JOVIA |
Resource Type | Journal Article |
Resource Relation | J. Virol. ; Vol/Issue: 53:2 |
Research Org | Institute of Virology, Moscow, USSR |
Subject | 550701 -- Microbiology-- Tracer Techniques; INFLUENZA VIRUSES-- CHEMICAL COMPOSITION;INFLUENZA VIRUSES-- INFECTIVITY; AMINO ACIDS;ANIMAL CELLS;ANTIBODIES;CARBON 14 COMPOUNDS;ELECTROPHORESIS;PROTEINS;RNA;STAPHYLOCOCCUS;TRACER TECHNIQUES;TRITIUM COMPOUNDS;URIDINE |
Related Subject | AZINES;BACTERIA;CARBOXYLIC ACIDS;HETEROCYCLIC COMPOUNDS;HYDROXY COMPOUNDS;ISOTOPE APPLICATIONS;LABELLED COMPOUNDS;MICROORGANISMS;NUCLEIC ACIDS;NUCLEOSIDES;NUCLEOTIDES;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;PARASITES;PYRIMIDINES;RIBOSIDES;URACILS;VIRUSES |
Description/Abstract | Phenotypically mixed virus yields, obtained by coinfection of MDCK cells with influenza A/WSN/33 and B/Lee/40 viruses, contained both A/WSN/33 and B/Lee/40 NP proteins, as revealed by polyacrylamide gel electrophoresis of the purified /sup 14/C-amino acids-labeled virus.^Virions were lysed with 0.6 M KCl-Triton X-100 buffer, and nucleocapsids were immunoprecipitated with antibodies against NP protein of influenza A virus.^Polyacrylamide gel electrophoresis of the immunoprecipitate revealed NP protein of A/WSN/33 but not of B/Lee/40 virus.^However, in similar experiments with the lysates of doubly infected cells, the band of B/Lee/40 NP protein was revealed in the polyacrylamide gel electrophoresis patterns of the immunoprecipitates.^In an attempt to analyze the RNA content of the immune complexes, the authors absorbed the lysates of doubly infected (/sup 3/H)uridine-labeled cells with protein A-containing Staphylococcus aureus covered with antibodies against the NP protein of influenza A virus.^RNA extracted from the immune complexes contained genomic RNA segments of both A/WSN/33 and B/Lee/40 viruses.^In control samples containing an artificial mixture of cell lysates separately infected with each virus, the analysis revealed homologous components (i.e., A/WSN/33 NP protein or RNA segments) in the immune complexes.^The results suggest the presence of phenotypically mixed nucleocapsids in the cells doubly infected with influenza A and B viruses; in the course of the virion formation, the nucleocapsids lacking the heterologous NP protein are selected. |
Country of Publication | United States |
Language | English |
Format | Pages: 679-683 |
System Entry Date | 2001 May 13 |
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