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Title Virions and intracellular nucleocapsids produced during mixed heterotypic influenza infection of MDCK cells
Creator/Author Sklyanskaya, E.I. ; Varich, N.L. ; Amvrosieva, T.V. ; Kaverin, N.V.
Publication Date1985 Feb 01
OSTI IdentifierOSTI ID: 5356234
Other Number(s)CODEN: JOVIA
Resource TypeJournal Article
Resource RelationJ. Virol. ; Vol/Issue: 53:2
Research OrgInstitute of Virology, Moscow, USSR
Subject550701 -- Microbiology-- Tracer Techniques; INFLUENZA VIRUSES-- CHEMICAL COMPOSITION;INFLUENZA VIRUSES-- INFECTIVITY; AMINO ACIDS;ANIMAL CELLS;ANTIBODIES;CARBON 14 COMPOUNDS;ELECTROPHORESIS;PROTEINS;RNA;STAPHYLOCOCCUS;TRACER TECHNIQUES;TRITIUM COMPOUNDS;URIDINE
Related SubjectAZINES;BACTERIA;CARBOXYLIC ACIDS;HETEROCYCLIC COMPOUNDS;HYDROXY COMPOUNDS;ISOTOPE APPLICATIONS;LABELLED COMPOUNDS;MICROORGANISMS;NUCLEIC ACIDS;NUCLEOSIDES;NUCLEOTIDES;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;PARASITES;PYRIMIDINES;RIBOSIDES;URACILS;VIRUSES
Description/Abstract Phenotypically mixed virus yields, obtained by coinfection of MDCK cells with influenza A/WSN/33 and B/Lee/40 viruses, contained both A/WSN/33 and B/Lee/40 NP proteins, as revealed by polyacrylamide gel electrophoresis of the purified /sup 14/C-amino acids-labeled virus.^Virions were lysed with 0.6 M KCl-Triton X-100 buffer, and nucleocapsids were immunoprecipitated with antibodies against NP protein of influenza A virus.^Polyacrylamide gel electrophoresis of the immunoprecipitate revealed NP protein of A/WSN/33 but not of B/Lee/40 virus.^However, in similar experiments with the lysates of doubly infected cells, the band of B/Lee/40 NP protein was revealed in the polyacrylamide gel electrophoresis patterns of the immunoprecipitates.^In an attempt to analyze the RNA content of the immune complexes, the authors absorbed the lysates of doubly infected (/sup 3/H)uridine-labeled cells with protein A-containing Staphylococcus aureus covered with antibodies against the NP protein of influenza A virus.^RNA extracted from the immune complexes contained genomic RNA segments of both A/WSN/33 and B/Lee/40 viruses.^In control samples containing an artificial mixture of cell lysates separately infected with each virus, the analysis revealed homologous components (i.e., A/WSN/33 NP protein or RNA segments) in the immune complexes.^The results suggest the presence of phenotypically mixed nucleocapsids in the cells doubly infected with influenza A and B viruses; in the course of the virion formation, the nucleocapsids lacking the heterologous NP protein are selected.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 679-683
System Entry Date2001 May 13

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