Energy Citations Database

Bibliographic Citation

 
Document
For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field.
For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability.
Title Early imaging of experimental myocardial infarction by intracoronary administration of /sup 131/I-labelled anticardiac myosin (Fab`)/sub 2/ fragments
Creator/Author Khaw, B.A. ; Gold, H.K. ; Leinbach, R.C. ; Fallon, J.T. ; Strauss, W. ; Pohost, G.M. ; Haber, E.
Publication Date1978 Dec 01
OSTI IdentifierOSTI ID: 6470532
Other Number(s)CODEN: CIRCA
Resource TypeJournal Article
Resource RelationCirculation ; Vol/Issue: 58:6
Research OrgMassachusetts General Hospital, Boston
Subject550601 -- Medicine-- Unsealed Radionuclides in Diagnostics ;550901 -- Pathology-- Tracer Techniques ;551001 -- Physiological Systems-- Tracer Techniques; ;ANTIBODIES-- RADIOPHARMACEUTICALS;MYOCARDIAL INFARCTION-- DIAGNOSIS;MYOCARDIUM-- SCINTISCANNING;MYOSIN-- ANTIBODIES;RADIOPHARMACEUTICALS-- SPECIFICITY; COMPARATIVE EVALUATIONS;DOGS;INTRAVENOUS INJECTION;IODINE 125;IODINE 131;LABELLED COMPOUNDS;THALLIUM 201
Related SubjectANIMALS;BETA DECAY RADIOISOTOPES;BETA-MINUS DECAY RADIOISOTOPES;BODY;CARDIOVASCULAR DISEASES;CARDIOVASCULAR SYSTEM;COUNTING TECHNIQUES;DAYS LIVING RADIOISOTOPES;DIAGNOSTIC TECHNIQUES;DISEASES;DRUGS;ELECTRON CAPTURE RADIOISOTOPES;GLOBULINS;HEART;HEAVY NUCLEI;INJECTION;INTAKE;INTERMEDIATE MASS NUCLEI;IODINE ISOTOPES;ISOMERIC TRANSITION ISOTOPES;ISOTOPES;LABELLED COMPOUNDS;MAMMALS;MUSCLES;NUCLEI;ODD-EVEN NUCLEI;ORGANIC COMPOUNDS;ORGANS;PROTEINS;RADIOACTIVE MATERIALS;RADIOISOTOPE SCANNING;RADIOISOTOPES;SECONDS LIVING RADIOISOTOPES;THALLIUM ISOTOPES;VERTEBRATES
Description/Abstract The feasibility of early imaging of myocardial infarcts by intracoronary injection of /sup 131/I-labelled cardiac myosin-specific antibody (Fab`)/sub 2/ was examined.^The left anterior descending coronary artery was occluded for 5 hs by a balloon catheter introduced through the carotid artery in 12 dogs.^The catheter was withdrawn and 1 mCi /sup 201/Tl was injected intravenously and 500 ..mu..Ci of /sup 131/I antibody were injected into the main left coronary artery.^Six of these animals demonstrated evidence of myocardial infarction by ECG and subsequent triphenyl-tetrazolium chloride staining, while the others did not.^In each of the infarcted animals, in vivo scintograms one-half h after injection of isotope showed uptake of /sup 131/I in the anteroapical region of the heart corresponding to the region of absent /sup 201/Tl uptake.^This relationship was confirmed in the excized hearts and in heart slices.^In slices, /sup 131/I uptake corresponded to regions that did not stain with triphenyltetrazolium chloride.^In the six animals that did not show evidence for infarction after coronary occlusion, uptake of /sup 131/I was not demonstrated, either in vivo or in excized specimens.^In four additional dogs subjected to the same procedure, /sup 125/I-labelled (Fab`)/sub 2/ from nonimmune IgG was injected simultaneously into the left main coronary artery with /sup 131/I-labelled canine myosin-specific antibody (Fab`)/sub 2/.^The ratio of uptake between infarct center and normal tissue was 34.3 +- 1.5 (mean +- SEM) for the specific antibody fragment as contrasted to 6.6 +- 0.4 for the nonimmune IgG fragment, indicating that intracoronary injection does not favor nonspecific sequestration of protein in regions of infarction.^Thus the intracoronary administration of myosin-specific antibody fragments leads to early and specific one-half h imaging of myocardial infarcts.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1137-1142
System Entry Date2001 May 13

Top