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Title Acute respiratory bronchiolitis: an ultrastructural and autoradiographic study of epithelial cell injury and renewal in Rhesus monkeys exposed to ozone
Creator/Author Castleman, W.L. ; Dungworth, D.L. ; Schwartz, L.W. ; Tyler, W.S.
Publication Date1980 Mar 01
OSTI IdentifierOSTI ID: 5993615
Other Number(s)CODEN: AJPAA
Resource TypeJournal Article
Resource RelationAm. J. Pathol. ; Vol/Issue: 98:3
Subject560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987) ;551001 -- Physiological Systems-- Tracer Techniques; ;BRONCHI-- ULTRASTRUCTURAL CHANGES;BRONCHITIS-- PATHOGENESIS;EPITHELIUM-- ULTRASTRUCTURAL CHANGES;OZONE-- BIOLOGICAL EFFECTS; AUTORADIOGRAPHY;MACROPHAGES;MONKEYS;NECROSIS;SCANNING ELECTRON MICROSCOPY;THYMIDINE;TRANSMISSION ELECTRON MICROSCOPY;TRITIUM COMPOUNDS
Related SubjectANIMAL CELLS;ANIMAL TISSUES;ANIMALS;AZINES;BODY;CONNECTIVE TISSUE CELLS;DISEASES;ELECTRON MICROSCOPY;HETEROCYCLIC COMPOUNDS;LABELLED COMPOUNDS;MAMMALS;MICROSCOPY;MORPHOLOGICAL CHANGES;NUCLEOSIDES;NUCLEOTIDES;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;PATHOLOGICAL CHANGES;PHAGOCYTES;PRIMATES;PYRIMIDINES;RESPIRATORY SYSTEM;RESPIRATORY SYSTEM DISEASES;RIBOSIDES;SOMATIC CELLS;TISSUES;VERTEBRATES
Description/Abstract The pathogenesis of acute respiratory bronchiolitis was examined in Rhesus monkeys exposed to 0.8 ppM ozone for 4 to 50 hours.^Epithelial injury and renewal were qualitatively and quantitatively characterized by correlated techniques of scanning and transmission electron microscopy as well as by light-microscopic autoradiography following labeling with tritiated thymidine.^Extensive degeneration and necrosis of Type 1 epithelial cells occurred on the respiratory bronchiolar wall during the initial 4 to 12 hours of exposure.^Increased numbers of labeled epithelial cells were present in this region after 18 hours of exposure, and the highest labeling index (18%) was measured after 50 hours of exposure.^Most (67 to 80%) of the labeled cells and all the mitotic epithelial cells (22) observed ultrastructurally were cuboidal bronchiolar epithelial cells.^Of the labeled epithelial cells, 20 to 33% were Type 2 epithelial cells.^After 50 hours of exposure the respiratory bronchiolar epithelium was hyperplastic.^The predominant inflammatory cell in respiratory bronchiolar exudate was the alveolar macrophage.^Monkeys that were exposed for 50 hours and allowed to recover in unozonized air for 7 days had incomplete resolution of respiratory bronchiolar epithelial hyperplasia.^The results indicate that Type 1 epithelial cells lining respiratory bronchioles are the cell types most sensitive to injury and that both cuboidal bronchiolar epithelial cells and Type 2 epithelial cells function as stem cells in epithelial renewal.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 811-840
System Entry Date2001 May 13

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