PETRAITIENE R, PETRAITIS V, KELAHER AM, SARAFANDI AA, SEIN T, BACHER J, WALSH TJ; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. M-370.

NCI, NIH, Bethesda, MD.

BACKGROUND: Invasive pulmonary aspergillosis (IPA) is an important cause of morbidity and mortality in immunocompromised patients. The introduction of the serum galactomannan (GM) EIA is an important advance in diagnosis of IPA. Little is known, however, about the expression of GM in bronchoalveolar lavage fluid (BAL) as a diagnostic marker of IPA. The correlation between BAL and serum GM concentrations, sensitivity to infection, and effect of antifungal therapy were investigated in persistently neutropenic rabbit model of IPA. METHODS: BAL was performed on each lung preparation by the instillation and subsequent withdrawal of 10 ml of sterile normal saline (NS) two times into the clamped trachea with a sterile 12-ml syringe. The lavage fluid was then centrifuged for 10 min at 1,500 x g. The supernatant was discarded, leaving the pellet, which was then resuspended in 2 ml of NS. BAL galactomannan was determined by the Platelia Aspergillus EIA one-stage immunoenzymatic sandwich microplate assay method. Study groups consisted of untreated controls (UC), rabbits treated with ravuconazole at 2.5 (RAV2.5), 5 (RAV5), and 10 (RAV10) mg/kg IV. Treatment was administered for 12 days starting 24 h after endotracheal inoculation of A. fumigatus conidia. RESULTS: The BAL GM index (GMI) showed linear correlation with serum GMI (r=0.6556, p</= 0.001). BAL GM showed 100% sensitivity in detection of pulmonary aspergillosis in infected UC rabbits (n=27), in comparison to 37% of BAL positive quantitative cultures (p=0.032) in the same rabbits. Rabbits treated with ravuconazole demonstrated significant dose dependent decrease in BAL GMI in comparison to UC (RAV5, p</= 0.05; RAV10, p Publication Types:

• Meeting Abstracts

Keywords:

• Animals
• Antigens
• Antigens, Fungal
• Aspergillosis, Allergic Bronchopulmonary
• Aspergillus
• Bronchoalveolar Lavage Fluid
• DNA Primers
• ER 30346
• Humans
• Immunoenzyme Techniques
• Lung
• Lung Diseases, Fungal
• Mannans
• Rabbits
• Sensitivity and Specificity
• Triazoles
• galactomannan
• immunology

Other ID:

• GWAIDS0024908

UI: 102264532

From Meeting Abstracts