|
Poster Presentation 7-10
Chitosanase Extraction and Purification by Expanded Bed Adsorption
E. S. Santos, R. Guirardello and T. T. Franco
Biochemical Engineering Laboratory School of Chemical Engineering State University of Campinas (UNICAMP) P.O.Box 6066 Campinas SP - 13081-970 Brazil
Telephone: (55) 19-3788-3966; Fax: (55) 19-3788-3965; E-mail: franco@feq.unicamp.br
This work deals with the purification of a fermentation broth of chitosanase using expanded bed adsorption (EBA). Initial batch uptake experiments with unclarified and clarified fermentation broth of chitosanase showed that equilibrium was reached in half an hour with adsorbent capacity of 0.119 U/mL of adsorbent and 0.133 U/mL of adsorbent, respectively. The packed bed purification using clarified chitosanase fermentation broth allowed us to obtain a chitosanase peak with a 7.6-fold purification factor and a 67.4% yield. These values were slightly higher than the values found for the expanded bed purification using both clarified and unclarified fermentation broth of chitosanase, respectively. A 6.9-fold purification factor and a 68.5% yield were obtained for the former and a 6.9-fold purification factor and a 67.9% yield were obtained for the latter. EBA using unclarified fermentation broth of chitosanase was successful for separating the chitosanase from the main contaminants of the broth.
|