D-11
Characterization of the Effect of Deoxynivalenol on Selected Male Reproductive Endpoints
R. L. Sprando1, T. F. Collins1, T. N. Black1, N. Olejnik1, D. I. Ruggles2, 1Division of Toxicology, 2Division of Mathematics
The effect of deoxynivalenol (DON) exposure on selected male reproductive endpoints was examined. Adult male rats received vehicle alone (distilled water) or DON (in distilled water) at one of four concentrations (0.5, 1.0. 2.5 or 5.0 mg/kg body weight) by gavage for 28 days. Body weight gain and final body weight of animals in the 5.0 mg/kg dose group and feed consumption in animals in the 2.5 mg/kg and 5.0 mg/kg dose groups were reduced compared to controls. Fluid consumption was not affected in any of the treated groups. Epididymal and seminal vesicle weights expressed per gram of body weight and per gram of brain weight were significantly lower than controls in the 2.5 and 5.0 mg/kg dose groups. Prostate weight expressed per gram of brain weight and per gram of body weight was significantly lower than controls in the 5.0 mg/kg dose group. Dose related decreases in homogenization resistant testicular spermatid numbers and cauda epididymal sperm numbers per gram of cauda epididymis were observed in the 5.0 mg/kg DON treatment group. Dose related changes in sperm motility were not observed across treatment groups however swimming speed was increased in the 2.5 mg/kg dose group. Serum FSH and LH concentrations were increased in a dose dependent manner across all treated groups while serum testosterone concentrations were decreased in a dose related manner across all dose groups. A slight increase in germ cell degeneration, sperm retention and abnormal nuclear morphology was observed in the 2.5 mg/kg and 5.0 mg/kg dose groups.
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Last updated on 2004-APR-02 by frf