Weiss, J. L. and J. B. Layzer (1995). Infestations of glochidia on fishes in the Barren River, Kentucky. American Malacological Bulletin. 11:153-159. We collected fish monthly from the Barren River, Kentucky, to assess glochidial infestations. Glochidia were encysted on 4.1% of the 2,510 fish of 43 species examined. Twenty-five fish species in 11 families were infested; 14 of these species are not known to be hosts of any of the 27 mussel species (Unionidae) occurring in the Barren River. Amblemine glochidia occurred on 19 species of fish. Eight species of fish were infested with anodontine glochidia, while lampsiline glochidia occurred on only five species. Differences in the degree of host specificity were striking among the Ambleminae. Glochidia of Amblema plicata (Say, 1817) occurred on 12 species of fish, whereas those of Quadrula pustulosa (I. Lea, 1831) were found only on channel catfish [Ictalurus punctatus (Rafinesque, 1818)]. Overlap in host fishes occurred between the Ambleminae and the other subfamilies, but not between the Anodontinae and Lampsilinae. Potential new hosts are identified for Lasmigona complanata (Barnes, 1823), Lasmigona costata (Rafinesque, 1820), Megalonaias nervosa (Rafinesque, 1820), A. plicata, and Pleurobema spp.