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DOI http://dx.doi.org/10.1210/jc.81.4.1533
Title Expression of{beta}{sub 1} integrins in human endometrial stromal and decidual cells
Creator/Author Shiokawa, Shigetatsu ; Yoshimura, Yasunori ; Nakamura, Yukio [Kyorin Univ. School of Medicine, Tokyo (Japan)][and others]
Publication Date1996 Apr 01
OSTI IdentifierOSTI ID: 530772
Other Number(s)JCEMAZ; ISSN 0021-972X
Resource TypeJournal Article
Resource RelationJournal of Clinical Endocrinology and Metabolism ; VOL. 81 ; ISSUE: 4 ; PBD: Apr 1996
Subject55 BIOLOGY AND MEDICINE, BASIC STUDIES ; ANIMAL CELLS; CELL PROLIFERATION; STRUCTURE-ACTIVITY RELATIONSHIPS; MEMBRANE PROTEINS; GENE REGULATION; RECEPTORS; EMBRYOS; ONTOGENESIS; UTERUS; CELL FLOW SYSTEMS; IMMUNOASSAY; ESTRADIOL; PREGNANCY; LTH; PROGESTERONE; ANTIBODIES
Description/Abstract The present study was undertaken to investigate the expression of{beta}{sub 1} integrins in human endometrium and decidua using flow cytometry, immunohistochemistry, and immunoprecipitation. Fluorescence-activated flow cytometry demonstrated the greater expression of the{beta}{sub 1},{alpha}{sub 1},{alpha}{sub 2}, and{alpha}{sub 5} subunits of the{beta}{sub 1} integrin family in cultured stromal cells from the midsecretory phase, than in those of the early proliferative phase. The addition of estradiol (E{sub 2}) and progesterone (P) to cultured stromal cells in the early proliferative phase increased the expression of{beta}{sub 1} integrins in vitro. Flow cytometry also demonstrated the expression of the{beta}{sub 1},{alpha}{sub 1},{alpha}{sub 2},{alpha}{sub 3},{alpha}{sub 5}, and{alpha}{sub 6} subunits of{beta}{sub 1} integrin family in cultured decidual cells, and the enriched-fraction of prolactin (PRL)-producing decidual cells isolated by Percoll gradients showed high levels of{beta}{sub 1} integrins expression. Immunohistochemistry confirmed the{beta}{sub 1} integrin cell surface phenotypes in cultured decidual cells observed by flow cytometry. In summary, the present study demonstrated that endometrial stromal and decidual cells expressed{beta}{sub 1} integrin subunits at their surfaces. The expression exhibited a variability throughout the menstrual cycles, being predominantly detected in the secretory phase, and was maintained highly in the decidua. Thus,{beta}{sub 1} integrins in human endometrium and decidua may be important in mediating the organization of extracellular matrix proteins derived from embryos during the early stage of implantation. 43 refs., 7 figs., 2 tabs.
Country of PublicationUnited States
LanguageEnglish
Formatpp. 1533-1540 ; PL:
System Entry Date2001 May 05

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