[Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats]
[Article in Chinese]
Postdoctoral scientific research afflux station, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
OBJECTIVE: The present study investigated the inhibitory effects of Chinese herb component sinapine on activity of acetylcholinesterase (AChE) in cerebral homogenate and blood serum of rats. METHOD: AChE was prepared from cerebral homogenate and blood serum of rats, respectively. Acetylcholinesterase activity assay kit and Chromatometry were used to detect the AChE activity. RESULT: Sinapine significantly inhibited AChE activity in vitro, with more effective on cerebral homogenate (IC50 3.66 micromol x L(-1)) than blood serum (IC50 22.1 micromol x L(-1)). CONCLUSION: Sinapine could significantly inhibit the cerebral AChE activity and may be a promising drug used for prevention and cure of Alzheimer's disease as a cholinesterase inhibitor.
PMID: 18589789 [PubMed - in process]