R = Retrieve links to Related sequences (neighbors).
Use the 
icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the 
icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the 
icon (if present) to retrieve links to the Protein Data Bank database.
WARNING: Descriptions of 127 database sequences were not reported due to the
limiting value of parameter V = 50.
>gi|136057|sp|P21820|TPIS_COPJA TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|99499|pir||A32187 triose-phosphate isomerase (EC 5.3.1.1) -
Coptis japonica >gi|556171|gb|AAB62730.1| (J04121) triosephosphate
isomerase [Coptis japonica]
Length = 253
Frame 3 hits (HSPs): _________________
Frame 2 hits (HSPs): ____________
Frame 1 hits (HSPs): ___________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 253
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 158..190
BLOCKS BL00171D: Triosephosphate isomerase prot 206..240
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 3..251
PRODOM PD001005: TPIS(59) TPIC(2) 3..251
PROSITE PEROXIDASE_2: Peroxidases active site si 52..63
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 387 (136.2 bits), Expect = 1.2e-49, Sum P(2) = 1.2e-49
Identities = 71/82 (86%), Positives = 78/82 (95%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGT+EEVKKIVT LNEA+VP EDVVEVVVSPP+VFLP VK+LLR DF
Sbjct: 1 MGRKFFVGGNWKCNGTSEEVKKIVTLLNEAEVPSEDVVEVVVSPPYVFLPFVKNLLRADF 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
HV+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 HVAAQNCWVKKGGAFTGEVSAE 82
Score = 156 (54.9 bits), Expect = 1.2e-49, Sum P(2) = 1.2e-49
Identities = 33/58 (56%), Positives = 43/58 (74%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNLGIPWVI+GHSE A +SNEFVG + A ++ K++ C+GETLEQRE+
Sbjct: 82 EMLVNLGIPWVILGHSERRALLN-ESNEFVGDKTAY-ALSQGLKVIACVGETLEQREA 137
Score = 54 (19.0 bits), Expect = 6.1e-39, Sum P(2) = 6.1e-39
Identities = 21/52 (40%), Positives = 29/52 (55%), Frame = +2
Query: 329 LGLLLVTL---KRRQLLK*NQMSLWENKVAYALQQGLKSYYHALGKLLNSVKAG 481
LG+ V L +RR LL + + + K AYAL QGLK +G+ L +AG
Sbjct: 87 LGIPWVILGHSERRALLNESNEFVGD-KTAYALSQGLK-VIACVGETLEQREAG 138
>gi|1351279|sp|P48495|TPIS_PETHY TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|602590|emb|CAA58230.1| (X83227) triosephosphate isomerase
[Petunia x hybrida]
Length = 254
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 254
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 158..190
BLOCKS BL00171D: Triosephosphate isomerase prot 206..240
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 3..251
PRODOM PD001005: TPIS(59) TPIC(2) 3..250
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 376 (132.4 bits), Expect = 1.2e-47, Sum P(2) = 1.2e-47
Identities = 68/82 (82%), Positives = 76/82 (92%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGT EEVKKI+ TLN A VP +DVVEVVVSPP+VFLP+VK+ LRPDF
Sbjct: 1 MGRKFFVGGNWKCNGTAEEVKKILATLNAADVPSQDVVEVVVSPPYVFLPLVKNELRPDF 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
HV+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 HVAAQNCWVKKGGAFTGEVSAE 82
Score = 148 (52.1 bits), Expect = 1.2e-47, Sum P(2) = 1.2e-47
Identities = 32/58 (55%), Positives = 41/58 (70%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNL IPWVI+GHSE A +SNEFVG A ++ K++ C+GETLE+RES
Sbjct: 82 EMLVNLSIPWVILGHSERRALLG-ESNEFVGD-KVAYALSQGLKVIACVGETLEERES 137
Score = 52 (18.3 bits), Expect = 1.4e-37, Sum P(2) = 1.4e-37
Identities = 13/28 (46%), Positives = 18/28 (64%), Frame = +2
Query: 398 NKVAYALQQGLKSYYHALGKLLNSVKAG 481
+KVAYAL QGLK +G+ L ++G
Sbjct: 112 DKVAYALSQGLK-VIACVGETLEERESG 138
>gi|136063|sp|P12863|TPIS_MAIZE TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|68426|pir||ISZMT triose-phosphate isomerase (EC 5.3.1.1) -
maize >gi|168647|gb|AAB81110.1| (L00371) triosephosphate isomerase
1 [Zea mays] >gi|217974|dbj|BAA00009.1| (D00012) triosephosphate
isomerase [Zea mays]
Length = 253
Frame 3 hits (HSPs): _________________
Frame 2 hits (HSPs): _______
Frame 1 hits (HSPs): ___________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 253
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 2..14
BLOCKS BL00171B: Triosephosphate isomerase prot 63..113
BLOCKS BL00171C: Triosephosphate isomerase prot 157..189
BLOCKS BL00171D: Triosephosphate isomerase prot 205..239
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 2..250
PRODOM PD001005: TPIS(59) TPIC(2) 2..250
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 368 (129.5 bits), Expect = 2.4e-47, Sum P(2) = 2.4e-47
Identities = 67/82 (81%), Positives = 76/82 (92%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGTT++V+KIV TLNE +VP DVVEVVVSPP+VFLPVVKS LR +F
Sbjct: 1 MGRKFFVGGNWKCNGTTDQVEKIVKTLNEGQVPPSDVVEVVVSPPYVFLPVVKSQLRQEF 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
HV+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 HVAAQNCWVKKGGAFTGEVSAE 82
Score = 153 (53.9 bits), Expect = 2.4e-47, Sum P(2) = 2.4e-47
Identities = 32/58 (55%), Positives = 42/58 (72%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNLG+PWVI+GHSE A +SNEFVG A ++ K++ C+GETLEQRE+
Sbjct: 82 EMLVNLGVPWVILGHSERRALLG-ESNEFVGD-KVAYALSQGLKVIACVGETLEQREA 137
Score = 55 (19.4 bits), Expect = 4.7e-37, Sum P(2) = 4.7e-37
Identities = 14/28 (50%), Positives = 18/28 (64%), Frame = +2
Query: 398 NKVAYALQQGLKSYYHALGKLLNSVKAG 481
+KVAYAL QGLK +G+ L +AG
Sbjct: 112 DKVAYALSQGLK-VIACVGETLEQREAG 138
>gi|1351272|sp|P48491|TPIS_ARATH TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|414550|gb|AAA03449.1| (U02949) cytosolic triose phosphate
isomerase [Arabidopsis thaliana] >gi|742408|prf||2009415A triose
phosphate isomerase [Arabidopsis thaliana]
Length = 254
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 254
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 158..190
BLOCKS BL00171D: Triosephosphate isomerase prot 206..240
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 3..251
PRODOM PD001005: TPIS(59) TPIC(2) 3..251
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 369 (129.9 bits), Expect = 1.7e-46, Sum P(2) = 1.7e-46
Identities = 68/82 (82%), Positives = 75/82 (91%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGNWKCNGT EEVKKIV TLNEA+VP +DVVEVVVSPP+VFLP+VKS LR DF
Sbjct: 1 MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDF 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
V+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 FVAAQNCWVKKGGAFTGEVSAE 82
Score = 144 (50.7 bits), Expect = 1.7e-46, Sum P(2) = 1.7e-46
Identities = 31/58 (53%), Positives = 41/58 (70%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNL IPWVI+GHSE A +S+EFVG A A+ K++ C+GETLE+RE+
Sbjct: 82 EMLVNLDIPWVILGHSERRAILN-ESSEFVGD-KVAYALAQGLKVIACVGETLEEREA 137
Score = 57 (20.1 bits), Expect = 2.3e-37, Sum P(2) = 2.3e-37
Identities = 19/43 (44%), Positives = 27/43 (62%), Frame = +2
Query: 353 KRRQLLK*NQMSLWE-NKVAYALQQGLKSYYHALGKLLNSVKAG 481
+RR +L N+ S + +KVAYAL QGLK +G+ L +AG
Sbjct: 98 ERRAIL--NESSEFVGDKVAYALAQGLK-VIACVGETLEEREAG 138
>gi|7076787|emb|CAB75902.1| (AL132975) cytosolic triosephosphatisomerase
[Arabidopsis thaliana]
Length = 254
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | || 254
0 50 100 150 200 250
Plus Strand HSPs:
Score = 369 (129.9 bits), Expect = 1.7e-46, Sum P(2) = 1.7e-46
Identities = 68/82 (82%), Positives = 75/82 (91%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGNWKCNGT EEVKKIV TLNEA+VP +DVVEVVVSPP+VFLP+VKS LR DF
Sbjct: 1 MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDF 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
V+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 FVAAQNCWVKKGGAFTGEVSAE 82
Score = 144 (50.7 bits), Expect = 1.7e-46, Sum P(2) = 1.7e-46
Identities = 31/58 (53%), Positives = 41/58 (70%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNL IPWVI+GHSE A +S+EFVG A A+ K++ C+GETLE+RE+
Sbjct: 82 EMLVNLDIPWVILGHSERRAILN-ESSEFVGD-KVAYALAQGLKVIACVGETLEEREA 137
Score = 57 (20.1 bits), Expect = 2.3e-37, Sum P(2) = 2.3e-37
Identities = 19/43 (44%), Positives = 27/43 (62%), Frame = +2
Query: 353 KRRQLLK*NQMSLWE-NKVAYALQQGLKSYYHALGKLLNSVKAG 481
+RR +L N+ S + +KVAYAL QGLK +G+ L +AG
Sbjct: 98 ERRAIL--NESSEFVGDKVAYALAQGLK-VIACVGETLEEREAG 138
>gi|1351270|sp|P48494|TPIS_ORYSA TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|478410|pir||JQ2255 triose-phosphate isomerase (EC 5.3.1.1) -
rice >gi|169821|gb|AAA18541.1| (M87064) triosephosphate isomerase
[Oryza sativa]
Length = 253
Frame 3 hits (HSPs): _________________
Frame 2 hits (HSPs): _________
Frame 1 hits (HSPs): ___________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 253
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 158..190
BLOCKS BL00171D: Triosephosphate isomerase prot 206..240
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 1..252
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 3..251
PRODOM PD001005: TPIS(59) TPIC(2) 3..251
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 346 (121.8 bits), Expect = 1.6e-44, Sum P(2) = 1.6e-44
Identities = 63/82 (76%), Positives = 72/82 (87%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGTT++V KIV LNE ++ DVVEVVVSPP+VFLPVVKS LRP+
Sbjct: 1 MGRKFFVGGNWKCNGTTDQVDKIVKILNEGQIASTDVVEVVVSPPYVFLPVVKSQLRPEI 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
V+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 QVAAQNCWVKKGGAFTGEVSAE 82
Score = 148 (52.1 bits), Expect = 1.6e-44, Sum P(2) = 1.6e-44
Identities = 32/58 (55%), Positives = 41/58 (70%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNL IPWVI+GHSE + +SNEFVG A ++ K++ C+GETLEQRES
Sbjct: 82 EMLVNLSIPWVILGHSERRSLLG-ESNEFVGD-KVAYALSQGLKVIACVGETLEQRES 137
Score = 53 (18.7 bits), Expect = 1.5e-34, Sum P(2) = 1.5e-34
Identities = 17/43 (39%), Positives = 25/43 (58%), Frame = +2
Query: 353 KRRQLLK*NQMSLWENKVAYALQQGLKSYYHALGKLLNSVKAG 481
+RR LL + + + KVAYAL QGLK +G+ L ++G
Sbjct: 98 ERRSLLGESNEFVGD-KVAYALSQGLK-VIACVGETLEQRESG 138
>gi|7437337|pir||T04134 triose-phosphate isomerase (EC 5.3.1.1) - rice
>gi|553107|gb|AAB63603.1| (L04967) triosephosphate isomerase [Oryza
sativa]
Length = 254
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | || 254
0 50 100 150 200 250
Plus Strand HSPs:
Score = 346 (121.8 bits), Expect = 1.6e-44, Sum P(2) = 1.6e-44
Identities = 63/82 (76%), Positives = 72/82 (87%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGTT++V KIV LNE ++ DVVEVVVSPP+VFLPVVKS LRP+
Sbjct: 1 MGRKFFVGGNWKCNGTTDQVDKIVKILNEGQIASTDVVEVVVSPPYVFLPVVKSQLRPEI 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
V+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 QVAAQNCWVKKGGAFTGEVSAE 82
Score = 148 (52.1 bits), Expect = 1.6e-44, Sum P(2) = 1.6e-44
Identities = 32/58 (55%), Positives = 41/58 (70%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNL IPWVI+GHSE + +SNEFVG A ++ K++ C+GETLEQRES
Sbjct: 82 EMLVNLSIPWVILGHSERRSLLG-ESNEFVGD-KVAYALSQGLKVIACVGETLEQRES 137
Score = 53 (18.7 bits), Expect = 1.5e-34, Sum P(2) = 1.5e-34
Identities = 17/43 (39%), Positives = 25/43 (58%), Frame = +2
Query: 353 KRRQLLK*NQMSLWENKVAYALQQGLKSYYHALGKLLNSVKAG 481
+RR LL + + + KVAYAL QGLK +G+ L ++G
Sbjct: 98 ERRSLLGESNEFVGD-KVAYALSQGLK-VIACVGETLEQRESG 138
>gi|2507469|sp|P34937|TPIS_HORVU TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|1785948|gb|AAB41052.1| (U83414) cytosolic triosephosphate
isomerase [Hordeum vulgare]
Length = 253
Frame 3 hits (HSPs): _________________
Frame 2 hits (HSPs): _________
Frame 1 hits (HSPs): ___________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 253
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 2..14
BLOCKS BL00171B: Triosephosphate isomerase prot 63..113
BLOCKS BL00171C: Triosephosphate isomerase prot 157..189
BLOCKS BL00171D: Triosephosphate isomerase prot 205..239
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 2..250
PRODOM PD001005: TPIS(59) TPIC(2) 2..250
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 335 (117.9 bits), Expect = 3.8e-43, Sum P(2) = 3.8e-43
Identities = 60/82 (73%), Positives = 71/82 (86%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGT E+V+ IV TLN ++ DVVEVVVSPP+VFLP+VK+ LRP+
Sbjct: 1 MGRKFFVGGNWKCNGTVEQVEAIVQTLNAGQIVSPDVVEVVVSPPYVFLPIVKAKLRPEI 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
V+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 QVAAQNCWVKKGGAFTGEVSAE 82
Score = 146 (51.4 bits), Expect = 3.8e-43, Sum P(2) = 3.8e-43
Identities = 30/58 (51%), Positives = 42/58 (72%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+ML NLG+PWVI+GHSE + +S+EFVG+ A A+ K++ C+GETLEQRE+
Sbjct: 82 EMLANLGVPWVILGHSERRSLLG-ESSEFVGE-KVAYALAQGLKVIACVGETLEQREA 137
Score = 58 (20.4 bits), Expect = 6.6e-34, Sum P(2) = 6.6e-34
Identities = 19/43 (44%), Positives = 25/43 (58%), Frame = +2
Query: 353 KRRQLLK*NQMSLWENKVAYALQQGLKSYYHALGKLLNSVKAG 481
+RR LL + + E KVAYAL QGLK +G+ L +AG
Sbjct: 98 ERRSLLGESSEFVGE-KVAYALAQGLK-VIACVGETLEQREAG 138
>gi|1174749|sp|P46226|TPIS_SECCE TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|1362177|pir||S53760 triose-phosphate isomerase (EC 5.3.1.1),
cytosolic - rye >gi|407525|emb|CAA81487.1| (Z26875) triosephosphate
isomerase [Secale cereale] >gi|1095493|prf||2109226A
triosephosphate isomerase [Secale cereale]
Length = 253
Frame 3 hits (HSPs): _________________
Frame 2 hits (HSPs): ____________
Frame 1 hits (HSPs): ___________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 253
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 2..14
BLOCKS BL00171B: Triosephosphate isomerase prot 63..113
BLOCKS BL00171C: Triosephosphate isomerase prot 157..189
BLOCKS BL00171D: Triosephosphate isomerase prot 205..239
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 2..250
PRODOM PD001005: TPIS(59) TPIC(2) 2..250
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 328 (115.5 bits), Expect = 4.9e-43, Sum P(2) = 4.9e-43
Identities = 59/82 (71%), Positives = 68/82 (82%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGT +V+ IV TLN ++ DVVEVVVSPP+VFLP VK LRP+
Sbjct: 1 MGRKFFVGGNWKCNGTVSQVETIVNTLNAGQIASPDVVEVVVSPPYVFLPTVKDKLRPEI 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
V+AQNCWV+KGGA+TGEVSAE
Sbjct: 61 QVAAQNCWVKKGGAFTGEVSAE 82
Score = 152 (53.5 bits), Expect = 4.9e-43, Sum P(2) = 4.9e-43
Identities = 32/58 (55%), Positives = 43/58 (74%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+MLVNLGIPWVI+GHSE + +S+EFVG+ A A+ K++ C+GETLEQRE+
Sbjct: 82 EMLVNLGIPWVILGHSERRSLLA-ESSEFVGE-KVAYALAQGLKVIACVGETLEQREA 137
Score = 60 (21.1 bits), Expect = 2.2e-33, Sum P(2) = 2.2e-33
Identities = 23/52 (44%), Positives = 30/52 (57%), Frame = +2
Query: 329 LGLLLVTL---KRRQLLK*NQMSLWENKVAYALQQGLKSYYHALGKLLNSVKAG 481
LG+ V L +RR LL + + E KVAYAL QGLK +G+ L +AG
Sbjct: 87 LGIPWVILGHSERRSLLAESSEFVGE-KVAYALAQGLK-VIACVGETLEQREAG 138
>gi|1351282|sp|P48497|TPIS_STELP TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|546735|gb|AAB30759.1| (S70730) triose phosphate isomerase,
TPI=5.3.1.1 [Stellaria longipes, Goldie, Peptide, 257 aa]
Length = 257
Frame 3 hits (HSPs): __________________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | | 257
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 159..191
BLOCKS BL00171D: Triosephosphate isomerase prot 207..241
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 1..256
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 167
PFAM TIM: Triosephosphate isomerase 3..253
PRODOM PD001005: TPIS(59) TPIC(2) 3..256
PROSITE TIM: Triosephosphate isomerase active si 165..175
__________________
Plus Strand HSPs:
Score = 304 (107.0 bits), Expect = 1.3e-38, Sum P(2) = 1.3e-38
Identities = 59/95 (62%), Positives = 69/95 (72%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKFFVGGNWKCNGT E V KIV TLNE + DVV VVVSPP+VFLP + L+ +
Sbjct: 1 MGRKFFVGGNWKCNGTQESVSKIVDTLNEPTIAANDVVTVVVSPPYVFLPDENAELKHEI 60
Query: 240 HVSAQNCWVRKGGAYTGEVSAENAC*FGNSLGYYW 344
V+AQNCWV+KGGA+TGEVSA+ +LG W
Sbjct: 61 QVAAQNCWVKKGGAFTGEVSAQMLA----NLGITW 91
Score = 134 (47.2 bits), Expect = 1.3e-38, Sum P(2) = 1.3e-38
Identities = 33/58 (56%), Positives = 40/58 (68%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKL--LPCIGETLEQRES 477
+ML NLGI WVI+GHSE +SNEFVGK A A+SE L + CIGE LE+RE+
Sbjct: 82 QMLANLGITWVILGHSERRTLLG-ESNEFVGK---KAAYAQSEGLGVIACIGELLEEREA 137
>gi|1174745|sp|P46225|TPIC_SECCE TRIOSEPHOSPHATE ISOMERASE, CHLOROPLAST
PRECURSOR (TIM) >gi|1363523|pir||S53761 triose-phosphate isomerase
(EC 5.3.1.1) precursor, chloroplast - rye
>gi|609262|emb|CAA83533.1| (Z32521) triosephosphate isomerase
[Secale cereale] >gi|1095494|prf||2109226B triosephosphate
isomerase [Secale cereale]
Length = 298
Frame 3 hits (HSPs): ______________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): __________
Annotated Domains: ___________________________________________
__________________________________________________
Database sequence: | | | | | | | 298
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 47..59
BLOCKS BL00171B: Triosephosphate isomerase prot 106..156
BLOCKS BL00171C: Triosephosphate isomerase prot 200..232
BLOCKS BL00171D: Triosephosphate isomerase prot 248..282
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 45..297
Entrez active site: BY SIMILARITY. 138
Entrez active site: BY SIMILARITY. 208
Entrez modified site: CHEMICAL NATURE NOT DETER 186
PFAM TIM: Triosephosphate isomerase 47..294
PRODOM PD001005: TPIS(59) TPIC(2) 48..297
PROSITE TIM: Triosephosphate isomerase active si 206..216
__________________
Plus Strand HSPs:
Score = 248 (87.3 bits), Expect = 1.2e-30, Sum P(2) = 1.2e-30
Identities = 47/79 (59%), Positives = 59/79 (74%), Frame = +3
Query: 69 KFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHVS 248
KFFVGGNWKCNGT E + K+V+ LN A + E V+VVV+PPF+++ VKS L VS
Sbjct: 48 KFFVGGNWKCNGTKESISKLVSDLNAATL--ESDVDVVVAPPFIYIDQVKSSLTDRIEVS 105
Query: 249 AQNCWVRKGGAYTGEVSAE 305
AQN W+ KGGA+TGE+SAE
Sbjct: 106 AQNTWIGKGGAFTGEISAE 124
Score = 114 (40.1 bits), Expect = 1.2e-30, Sum P(2) = 1.2e-30
Identities = 24/58 (41%), Positives = 38/58 (65%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+ LV++G WVI+GHSE + +EF+GK A +++ K++ CIGE LE+RE+
Sbjct: 124 EQLVDIGCQWVILGHSERRHVIG-EDDEFIGK-KAAYALSQNLKVMACIGELLEEREA 179
Score = 50 (17.6 bits), Expect = 5.8e-24, Sum P(2) = 5.8e-24
Identities = 13/27 (48%), Positives = 16/27 (59%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
K AYAL Q LK +G+LL +AG
Sbjct: 155 KAAYALSQNLK-VMACIGELLEEREAG 180
>gi|1351271|sp|P48496|TPIC_SPIOL TRIOSEPHOSPHATE ISOMERASE, CHLOROPLAST
PRECURSOR (TIM) >gi|1084309|pir||S52032 triose-phosphate isomerase
(EC 5.3.1.1) precursor, chloroplast - spinach
>gi|806312|gb|AAA66289.1| (L36387) triosephosphate isomerase,
chloroplast isozyme [Spinacia oleracea]
Length = 322
Frame 3 hits (HSPs): ______________
Frame 2 hits (HSPs): _______
Frame 1 hits (HSPs): __________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | | | 322
0 50 100 150 200 250 300
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 71..83
BLOCKS BL00171B: Triosephosphate isomerase prot 130..180
BLOCKS BL00171C: Triosephosphate isomerase prot 224..256
BLOCKS BL00171D: Triosephosphate isomerase prot 272..306
Entrez active site: BY SIMILARITY. 162
Entrez active site: BY SIMILARITY. 232
PFAM TIM: Triosephosphate isomerase 71..318
PRODOM PD116792: TPIC_SPIOL 1..70
PRODOM PD001005: TPIS(59) TPIC(2) 72..321
PROSITE TIM: Triosephosphate isomerase active si 230..240
__________________
Plus Strand HSPs:
Score = 253 (89.1 bits), Expect = 2.4e-30, Sum P(2) = 2.4e-30
Identities = 49/92 (53%), Positives = 62/92 (67%), Frame = +3
Query: 69 KFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHVS 248
KFFVGGNWKCNGT E + K+V+ LN A + + V+VVV+PPFV++ VKS L +S
Sbjct: 72 KFFVGGNWKCNGTKESITKLVSDLNSATLEAD--VDVVVAPPFVYIDQVKSSLTGRVEIS 129
Query: 249 AQNCWVRKGGAYTGEVSAENAC*FGNSLGYYW 344
AQNCW+ KGGA+TGE+S E LG W
Sbjct: 130 AQNCWIGKGGAFTGEISVEQL----KDLGCQW 157
Score = 106 (37.3 bits), Expect = 2.4e-30, Sum P(2) = 2.4e-30
Identities = 24/59 (40%), Positives = 35/59 (59%), Frame = +1
Query: 301 LKMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
++ L +LG WVI+GHSE + NEF+GK A + ++ CIGE LE+RE+
Sbjct: 147 VEQLKDLGCQWVILGHSERRHVIG-EQNEFIGK-KAAYALNQGVGVIACIGELLEEREA 203
Score = 49 (17.2 bits), Expect = 2.2e-24, Sum P(2) = 2.2e-24
Identities = 15/43 (34%), Positives = 22/43 (51%), Frame = +2
Query: 353 KRRQLLK*NQMSLWENKVAYALQQGLKSYYHALGKLLNSVKAG 481
+RR ++ Q K AYAL QG+ +G+LL +AG
Sbjct: 164 ERRHVIG-EQNEFIGKKAAYALNQGV-GVIACIGELLEEREAG 204
>gi|4803926|gb|AAD29799.1|AC006264_7 (AC006264) putative triosephosphate
isomerase [Arabidopsis thaliana]
>gi|7839391|gb|AAF70259.1|AF247559_1 (AF247559) triosephosphate
isomerase [Arabidopsis thaliana]
Length = 315
Frame 3 hits (HSPs): ______________
Frame 2 hits (HSPs): _____
Frame 1 hits (HSPs): _________
__________________________________________________
Database sequence: | | | | | | | | 315
0 50 100 150 200 250 300
Plus Strand HSPs:
Score = 239 (84.1 bits), Expect = 7.1e-29, Sum P(2) = 7.1e-29
Identities = 47/92 (51%), Positives = 60/92 (65%), Frame = +3
Query: 69 KFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHVS 248
KFFVGGNWKCNGT + + K+++ LN A + + V+VVVSPPFV++ VKS L +S
Sbjct: 65 KFFVGGNWKCNGTKDSIAKLISDLNSATLEAD--VDVVVSPPFVYIDQVKSSLTDRIDIS 122
Query: 249 AQNCWVRKGGAYTGEVSAENAC*FGNSLGYYW 344
QN WV KGGA+TGE+S E LG W
Sbjct: 123 GQNSWVGKGGAFTGEISVEQL----KDLGCKW 150
Score = 106 (37.3 bits), Expect = 7.1e-29, Sum P(2) = 7.1e-29
Identities = 28/59 (47%), Positives = 37/59 (62%), Frame = +1
Query: 301 LKMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKL--LPCIGETLEQRE 474
++ L +LG WVI+GHSE K +EF+GK A A SE L + CIGE LE+RE
Sbjct: 140 VEQLKDLGCKWVILGHSERRHVIGEK-DEFIGK---KAAYALSEGLGVIACIGEKLEERE 195
Query: 475 S 477
+
Sbjct: 196 A 196
Score = 42 (14.8 bits), Expect = 3.5e-22, Sum P(2) = 3.5e-22
Identities = 11/27 (40%), Positives = 15/27 (55%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
K AYAL +GL +G+ L +AG
Sbjct: 172 KAAYALSEGL-GVIACIGEKLEEREAG 197
>gi|7650502|gb|AAF66071.1|AF257322_1 (AF257322) triosephosphate isomerase
[Fragaria x ananassa]
Length = 314
Frame 3 hits (HSPs): _____________
Frame 2 hits (HSPs): _____
Frame 1 hits (HSPs): __________
__________________________________________________
Database sequence: | | | | | | | | 314
0 50 100 150 200 250 300
Plus Strand HSPs:
Score = 241 (84.8 bits), Expect = 7.9e-28, Sum P(2) = 7.9e-28
Identities = 46/79 (58%), Positives = 58/79 (73%), Frame = +3
Query: 69 KFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHVS 248
KFFVGGNWKCNGT + + K+V+ LN AK+ E V+VVV+PPF++L VKS L +S
Sbjct: 64 KFFVGGNWKCNGTKDLISKLVSDLNSAKL--EPDVDVVVAPPFLYLDQVKSSLTDRIEIS 121
Query: 249 AQNCWVRKGGAYTGEVSAE 305
QN WV KGGA+TGE+S E
Sbjct: 122 GQNSWVAKGGAFTGEISVE 140
Score = 94 (33.1 bits), Expect = 7.9e-28, Sum P(2) = 7.9e-28
Identities = 24/59 (40%), Positives = 37/59 (62%), Frame = +1
Query: 301 LKMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKL--LPCIGETLEQRE 474
++ L ++G WVI+GHSE + ++F+GK A A +E L + CIGE LE+RE
Sbjct: 139 VEQLKDIGRKWVILGHSERRHVIG-EDDQFIGK---KAAYALNEGLGVIACIGEKLEERE 194
Query: 475 S 477
+
Sbjct: 195 A 195
Score = 42 (14.8 bits), Expect = 2.2e-22, Sum P(2) = 2.2e-22
Identities = 11/27 (40%), Positives = 15/27 (55%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
K AYAL +GL +G+ L +AG
Sbjct: 171 KAAYALNEGL-GVIACIGEKLEEREAG 196
>gi|136066|sp|P00939|TPIS_RABIT TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|68422|pir||ISRBT triose-phosphate isomerase (EC 5.3.1.1) -
rabbit >gi|223374|prf||0801190A isomerase,triosephosphate [Homo
sapiens]
Length = 248
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 248
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 4..16
BLOCKS BL00171B: Triosephosphate isomerase prot 63..113
BLOCKS BL00171C: Triosephosphate isomerase prot 157..189
BLOCKS BL00171D: Triosephosphate isomerase prot 205..239
Entrez active site: BY SIMILARITY. 95
Entrez active site 165
Entrez amidation site: DE. 15
Entrez amidation site: DE. 71
PFAM TIM: Triosephosphate isomerase 4..248
PRODOM PD001005: TPIS(59) TPIC(2) 4..247
PROSITE TIM: Triosephosphate isomerase active si 163..173
__________________
Plus Strand HSPs:
Score = 200 (70.4 bits), Expect = 9.5e-24, Sum P(2) = 9.5e-24
Identities = 38/78 (48%), Positives = 52/78 (66%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++TTLN AKVP + EVV +PP ++ + L P V
Sbjct: 4 RKFFVGGNWKMNGRKKNLGELITTLNAAKVPAD--TEVVCAPPTAYIDFARQKLDPKIAV 61
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 62 AAQNCYKVTNGAFTGEIS 79
Score = 96 (33.8 bits), Expect = 9.5e-24, Sum P(2) = 9.5e-24
Identities = 24/57 (42%), Positives = 36/57 (63%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKL--LPCIGETLEQRES 477
M+ + G WV++GHSE F +S+E +G+ A A SE L + CIGE L++RE+
Sbjct: 82 MIKDCGATWVVLGHSERRHVFG-ESDELIGQ---KVAHALSEGLGVIACIGEKLDEREA 136
Score = 42 (14.8 bits), Expect = 4.1e-18, Sum P(2) = 4.1e-18
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 112 KVAHALSEGL-GVIACIGEKLDEREAG 137
>gi|1351281|sp|P48501|TPIS_SCHMA TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|283520|pir||A38233 triose-phosphate isomerase (EC 5.3.1.1) -
fluke (Schistosoma mansoni) >gi|161129|gb|AAA29941.1| (M83294)
triose phosphate isomerase [Schistosoma mansoni]
>gi|552248|gb|AAA29919.1| (L07286) triose phosphate isomerase
[Schistosoma mansoni]
Length = 253
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ___________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 253
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 5..17
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 161..193
BLOCKS BL00171D: Triosephosphate isomerase prot 209..243
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 3..252
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 169
PFAM TIM: Triosephosphate isomerase 5..252
PRODOM PD001005: TPIS(59) TPIC(2) 5..251
PROSITE TIM: Triosephosphate isomerase active si 167..177
__________________
Plus Strand HSPs:
Score = 197 (69.3 bits), Expect = 9.5e-24, Sum P(2) = 9.5e-24
Identities = 37/78 (47%), Positives = 55/78 (70%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG+ ++ K++ L+EA +D EV+++PP VFL ++ L+ + HV
Sbjct: 5 RKFFVGGNWKMNGSRDDNDKLLKLLSEAHF--DDNTEVLIAPPSVFLHEIRKSLKKEIHV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 63 AAQNCYKVSKGAFTGEIS 80
Score = 99 (34.8 bits), Expect = 9.5e-24, Sum P(2) = 9.5e-24
Identities = 23/57 (40%), Positives = 34/57 (59%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE F +S+E + + A A ++ CIGETL +RES
Sbjct: 83 MIRDIGCDWVILGHSERRNIFG-ESDELIAE-KVQHALAEGLSVIACIGETLSERES 137
>gi|2501312|sp|Q27775|TPIS_SCHJA TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|1255223|gb|AAC47393.1| (U50847) triosephosphate isomerase
[Schistosoma japonicum]
Length = 252
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ___________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 252
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 5..17
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 161..193
BLOCKS BL00171D: Triosephosphate isomerase prot 209..243
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 169
PFAM TIM: Triosephosphate isomerase 5..252
PRODOM PD001005: TPIS(59) TPIC(2) 5..251
PROSITE TIM: Triosephosphate isomerase active si 167..177
__________________
Plus Strand HSPs:
Score = 189 (66.5 bits), Expect = 1.9e-23, Sum P(2) = 1.9e-23
Identities = 36/78 (46%), Positives = 54/78 (69%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG+ + K ++ +L+EA G+ E++++ PFV+L V+ L + HV
Sbjct: 5 RKFFVGGNWKMNGSRADNKVLIHSLSEAHFCGD--TEILIAAPFVYLNEVRQSLAKEIHV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 63 AAQNCYKVPKGAFTGEIS 80
Score = 104 (36.6 bits), Expect = 1.9e-23, Sum P(2) = 1.9e-23
Identities = 24/57 (42%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE + F +S+E V + A A ++ CIGETL +RES
Sbjct: 83 MIKDVGCDWVILGHSERRSIFN-ESDELVAE-KVQHALAEGLSVVACIGETLSERES 137
>gi|1174082|gb|AAC47855.1| (U43343) triosephosphate isomerase [Schistosoma
japonicum]
Length = 252
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ___________
__________________________________________________
Database sequence: | | | | | || 252
0 50 100 150 200 250
Plus Strand HSPs:
Score = 189 (66.5 bits), Expect = 3.1e-23, Sum P(2) = 3.1e-23
Identities = 36/78 (46%), Positives = 54/78 (69%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG+ + K ++ +L+EA G+ E++++ PFV+L V+ L + HV
Sbjct: 5 RKFFVGGNWKMNGSRADNKVLIHSLSEAHFCGD--TEILIAAPFVYLNEVRQSLAKEIHV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 63 AAQNCYKVPKGAFTGEIS 80
Score = 102 (35.9 bits), Expect = 3.1e-23, Sum P(2) = 3.1e-23
Identities = 24/57 (42%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE + F +S+E V + A A ++ CIGETL +RES
Sbjct: 83 MIKDVGCDWVILGHSERRSIFN-ESDELVAE-KVQHALAGGLSVVACIGETLSERES 137
>gi|4507645 ref|NP_000356.1| triosephosphate isomerase 1
>gi|136060|sp|P00938|TPIS_HUMAN TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|68420|pir||ISCZT1 triose-phosphate isomerase (EC 5.3.1.1) 1 -
chimpanzee >gi|2144541|pir||ISHUT triose-phosphate isomerase (EC
5.3.1.1) - human >gi|37247|emb|CAA49379.1| (X69723) triosephosphate
isomerase [Homo sapiens] >gi|176960|gb|AAA35438.1| (M57946)
triose-phosphate isomerase [Pan troglodytes]
>gi|339841|gb|AAB59511.1| (M10036) triosephosphate isomerase (EC
5.3.1.1) [Homo sapiens] >gi|1200507|gb|AAB51316.1| (U47924)
triosephosphate isomerase [Homo sapiens]
Length = 249
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 249
0 50 100 150 200
Plus Strand HSPs:
Score = 194 (68.3 bits), Expect = 5.1e-23, Sum P(2) = 5.1e-23
Identities = 37/78 (47%), Positives = 51/78 (65%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AKVP + EVV +PP ++ + L P V
Sbjct: 5 RKFFVGGNWKMNGRKQSLGELIGTLNAAKVPAD--TEVVCAPPTAYIDFARQKLDPKIAV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 63 AAQNCYKVTNGAFTGEIS 80
Score = 95 (33.4 bits), Expect = 5.1e-23, Sum P(2) = 5.1e-23
Identities = 21/57 (36%), Positives = 34/57 (59%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ + G WV++GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 83 MIKDCGATWVVLGHSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 137
Score = 41 (14.4 bits), Expect = 2.2e-17, Sum P(2) = 2.2e-17
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 113 KVAHALAEGL-GVIACIGEKLDEREAG 138
>gi|136062|sp|P15426|TPIS_MACMU TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|68421|pir||ISMQTR triose-phosphate isomerase (EC 5.3.1.1) -
rhesus macaque >gi|342348|gb|AAA36922.1| (M37572) triosephosphate
isomerase [Macaca mulatta] >gi|1200106|emb|CAA30823.1| (X08023)
triosephosphate isomerase [Macaca mulatta]
Length = 249
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 249
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 4..16
BLOCKS BL00171B: Triosephosphate isomerase prot 63..113
BLOCKS BL00171C: Triosephosphate isomerase prot 157..189
BLOCKS BL00171D: Triosephosphate isomerase prot 205..239
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 4..248
PRODOM PD001005: TPIS(59) TPIC(2) 4..247
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 194 (68.3 bits), Expect = 5.1e-23, Sum P(2) = 5.1e-23
Identities = 37/78 (47%), Positives = 51/78 (65%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AKVP + EVV +PP ++ + L P V
Sbjct: 5 RKFFVGGNWKMNGRKQNLGELIGTLNAAKVPAD--TEVVCAPPTAYIDFARQKLDPKIAV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 63 AAQNCYKVTNGAFTGEIS 80
Score = 95 (33.4 bits), Expect = 5.1e-23, Sum P(2) = 5.1e-23
Identities = 21/57 (36%), Positives = 34/57 (59%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ + G WV++GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 83 MIKDCGATWVVLGHSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 137
Score = 41 (14.4 bits), Expect = 2.2e-17, Sum P(2) = 2.2e-17
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 113 KVAHALAEGL-GVIACIGEKLDEREAG 138
>gi|999892|pdb|1HTI|A Chain A, Triosephosphate Isomerase (Tim) (E.C.5.3.1.1)
Complexed With 2-Phosphoglycolic Acid >gi|999893|pdb|1HTI|B Chain
B, Triosephosphate Isomerase (Tim) (E.C.5.3.1.1) Complexed With
2-Phosphoglycolic Acid
Length = 248
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 248
0 50 100 150 200
Plus Strand HSPs:
Score = 194 (68.3 bits), Expect = 5.1e-23, Sum P(2) = 5.1e-23
Identities = 37/78 (47%), Positives = 51/78 (65%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AKVP + EVV +PP ++ + L P V
Sbjct: 4 RKFFVGGNWKMNGRKQSLGELIGTLNAAKVPAD--TEVVCAPPTAYIDFARQKLDPKIAV 61
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 62 AAQNCYKVTNGAFTGEIS 79
Score = 95 (33.4 bits), Expect = 5.1e-23, Sum P(2) = 5.1e-23
Identities = 21/57 (36%), Positives = 34/57 (59%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ + G WV++GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 82 MIKDCGATWVVLGHSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 136
Score = 41 (14.4 bits), Expect = 2.2e-17, Sum P(2) = 2.2e-17
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 112 KVAHALAEGL-GVIACIGEKLDEREAG 137
>gi|136061|sp|P00941|TPIS_LATCH TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|68425|pir||ISLAT triose-phosphate isomerase (EC 5.3.1.1) -
coelacanth
Length = 247
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 62..112
BLOCKS BL00171C: Triosephosphate isomerase prot 156..188
BLOCKS BL00171D: Triosephosphate isomerase prot 204..238
Entrez active site: BY SIMILARITY. 94
Entrez active site: BY SIMILARITY. 164
PFAM TIM: Triosephosphate isomerase 3..247
PRODOM PD001005: TPIS(59) TPIC(2) 3..246
PROSITE TIM: Triosephosphate isomerase active si 162..172
__________________
Plus Strand HSPs:
Score = 187 (65.8 bits), Expect = 1.7e-22, Sum P(2) = 1.7e-22
Identities = 37/78 (47%), Positives = 51/78 (65%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AKVP E+V +PP +L + + P F V
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIQTLNAAKVPFTG--EIVCAPPEAYLDFARLKVDPKFGV 60
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 61 AAQNCYKVSKGAFTGEIS 78
Score = 97 (34.1 bits), Expect = 1.7e-22, Sum P(2) = 1.7e-22
Identities = 24/57 (42%), Positives = 37/57 (64%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKL--LPCIGETLEQRES 477
M+ + G+ WVI+GHSE F +S+E +G+ + A SE L + CIGE L++RE+
Sbjct: 81 MIKDCGVTWVILGHSERRHVFG-ESDELIGQ---KVSHALSEGLGVVACIGEKLDEREA 135
>gi|1351280|sp|P48500|TPIS_RAT TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|538426|gb|AAA42278.1| (L36250) triosephosphate isomerase
[Rattus norvegicus]
Length = 249
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 249
0 50 100 150 200
__________________
Annotated Domains:
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 4..248
PRODOM PD001005: TPIS(59) TPIC(2) 4..247
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 187 (65.8 bits), Expect = 1.7e-22, Sum P(2) = 1.7e-22
Identities = 36/78 (46%), Positives = 51/78 (65%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AK+P + EVV +PP ++ + L P V
Sbjct: 5 RKFFVGGNWKMNGRKKCLGELICTLNAAKLPAD--TEVVCAPPTAYIDFARQKLDPKIAV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 63 AAQNCYKVTNGAFTGEIS 80
Score = 97 (34.1 bits), Expect = 1.7e-22, Sum P(2) = 1.7e-22
Identities = 21/57 (36%), Positives = 36/57 (63%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ +LG WV++GHSE F +S+E +G+ A + +++ CIGE L++RE+
Sbjct: 83 MIKDLGATWVVLGHSERRHIFG-ESDELIGQ-KVNHALSEGLEVIACIGEKLDEREA 137
>gi|6979056|gb|AAF34328.1|AF063802_1 (AF063802) triosephosphate
isomerase/glyceraldehyde-3-phosphate dehydrogenase precursor
[Odontella sinensis]
Length = 615
Frame 3 hits (HSPs): _______
Frame 2 hits (HSPs): ___
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | 615
0 150 300 450 600
Plus Strand HSPs:
Score = 209 (73.6 bits), Expect = 2.1e-22, Sum P(2) = 2.1e-22
Identities = 40/80 (50%), Positives = 55/80 (68%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWKCNG+ ++V +V+ LN++ + + EVVV P V++ V LR D V
Sbjct: 30 RKFFVGGNWKCNGSVQQVNDLVSMLNQSTLSTD--TEVVVCPSQVYVQGVSEKLRKDIGV 87
Query: 246 SAQNCWVRKGGAYTGEVSAE 305
AQ+CWV+ GA+TGE SA+
Sbjct: 88 GAQDCWVQGNGAFTGETSAD 107
Score = 93 (32.7 bits), Expect = 2.1e-22, Sum P(2) = 2.1e-22
Identities = 22/57 (38%), Positives = 33/57 (57%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
ML ++G+ WV+IGHSE E ++E V + A + K++ C GE LE RE+
Sbjct: 108 MLADMGVGWVVIGHSERRGKGE--ADEEVAR-KAKYALDKGLKVIACCGEPLESREA 161
Score = 41 (14.4 bits), Expect = 5.5e-17, Sum P(2) = 5.5e-17
Identities = 12/27 (44%), Positives = 15/27 (55%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
K YAL +GLK G+ L S +AG
Sbjct: 137 KAKYALDKGLK-VIACCGEPLESREAG 162
>gi|6678413 ref|NP_033441.1| triosephosphate isomerase
>gi|2851390|sp|P17751|TPIS_MOUSE TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|3287374|gb|AAC36016.1| (AC002397) TPI [Mus musculus]
Length = 249
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 249
0 50 100 150 200
Plus Strand HSPs:
Score = 181 (63.7 bits), Expect = 4.4e-22, Sum P(2) = 4.4e-22
Identities = 36/78 (46%), Positives = 49/78 (62%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN A VP EVV +PP ++ + L P V
Sbjct: 5 RKFFVGGNWKMNGRKKCLGELICTLNAANVPAG--TEVVCAPPTAYIDFARQKLDPKIAV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 63 AAQNCYKVTNGAFTGEIS 80
Score = 99 (34.8 bits), Expect = 4.4e-22, Sum P(2) = 4.4e-22
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ +LG WV++GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 83 MIKDLGATWVVLGHSERRHVFG-ESDELIGQ-KVSHALAEGLGVIACIGEKLDEREA 137
>gi|7274156|gb|AAF44720.1|AF063107_1 (AF063107) triosephosphate isomerase +
glyceraldehyde-3-phosphate dehydrogenase [Achlya bisexualis]
Length = 596
Frame 3 hits (HSPs): ________
Frame 1 hits (HSPs): _____
__________________________________________________
Database sequence: | | | | | 596
0 150 300 450
Plus Strand HSPs:
Score = 220 (77.4 bits), Expect = 7.1e-22, Sum P(2) = 7.1e-22
Identities = 45/82 (54%), Positives = 55/82 (67%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
+ RKFFVGGNWKCNG+ + + +V TLN A +P VEVVV+P + VKS LR D
Sbjct: 9 VARKFFVGGNWKCNGSLSQAQSLVDTLNTATIPKN--VEVVVAPASIHALSVKSSLRKDI 66
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
VS Q+ W R GA+TGEVSAE
Sbjct: 67 GVSGQDTWSRGNGAFTGEVSAE 88
Score = 76 (26.8 bits), Expect = 7.1e-22, Sum P(2) = 7.1e-22
Identities = 22/58 (37%), Positives = 32/58 (55%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+ML + GI + IIGHSE E +NE V A ++ ++ CIGET +RE+
Sbjct: 88 EMLKDAGINYTIIGHSERRQKGE--TNEEVAS-KAGYALSKGVSVIACIGETKIEREA 142
>gi|68423|pir||ISMST triose-phosphate isomerase (EC 5.3.1.1) - mouse
>gi|54855|emb|CAA37420.1| (X53333) triosephosphate isomerase [Mus
musculus]
Length = 249
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
Annotated Domains: _ ___ ____
__________________________________________________
Database sequence: | | | | | | 249
0 50 100 150 200
__________________
Annotated Domains:
Entrez active site: His, Glu 96
Entrez active site: His, Glu 166
PROSITE ATPASE_DELTA: ATP synthase delta (OSCP) 221..240
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 176 (62.0 bits), Expect = 1.5e-21, Sum P(2) = 1.5e-21
Identities = 35/78 (44%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN A VP EVV +PP ++ + L P V
Sbjct: 5 RKFFVGGNWKMNGRKKCLGELICTLNAANVPAG--TEVVCAPPTAYIDFARQKLDPKIAV 62
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ G +TGE+S
Sbjct: 63 AAQNCYKVTNGPFTGEIS 80
Score = 99 (34.8 bits), Expect = 1.5e-21, Sum P(2) = 1.5e-21
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ +LG WV++GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 83 MIKDLGATWVVLGHSERRHVFG-ESDELIGQ-KVSHALAEGLGVIACIGEKLDEREA 137
>gi|1729998|sp|Q10657|TPIS_CAEEL TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|1036784|gb|AAA79846.1| (U23081) triosephosphate isomerase
[Caenorhabditis elegans]
Length = 247
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 62..112
BLOCKS BL00171C: Triosephosphate isomerase prot 156..188
BLOCKS BL00171D: Triosephosphate isomerase prot 204..238
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 1..246
Entrez active site: BY SIMILARITY. 94
Entrez active site: BY SIMILARITY. 164
PFAM TIM: Triosephosphate isomerase 3..247
PRODOM PD001005: TPIS(59) TPIC(2) 3..246
PROSITE TIM: Triosephosphate isomerase active si 162..172
__________________
Plus Strand HSPs:
Score = 177 (62.3 bits), Expect = 3.9e-21, Sum P(2) = 3.9e-21
Identities = 40/80 (50%), Positives = 49/80 (61%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGNWK NG V IVT LN + V+VVV+PP +L KS L+
Sbjct: 1 MTRKFFVGGNWKMNGDYASVDGIVTFLNASA--DNSSVDVVVAPPAPYLAYAKSKLKAGV 58
Query: 240 HVSAQNCWVRKGGAYTGEVS 299
V+AQNC+ GA+TGE+S
Sbjct: 59 LVAAQNCYKVPKGAFTGEIS 78
Score = 94 (33.1 bits), Expect = 3.9e-21, Sum P(2) = 3.9e-21
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ +LG+ WVI+GHSE F +S+ + + + A K++ CIGE LE+RE+
Sbjct: 81 MIKDLGLEWVILGHSERRHVFG-ESDALIAE-KTVHALEAGIKVVFCIGEKLEEREA 135
>gi|7509442|pir||T26493 hypothetical protein Y17G7B.7 - Caenorhabditis elegans
>gi|3947595|emb|CAA19447.1| (AL023828) predicted using
Genefinder~similar to Triosephosphate isomerase~cDNA EST
EMBL:D74325 comes from this gene~cDNA EST EMBL:D75380 comes from
this gene~cDNA EST EMBL:D76016 comes from this gene~cDNA EST
EMBL:C08776 comes from this gene~cDNA EST >
Length = 247
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 177 (62.3 bits), Expect = 3.9e-21, Sum P(2) = 3.9e-21
Identities = 40/80 (50%), Positives = 49/80 (61%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGNWK NG V IVT LN + V+VVV+PP +L KS L+
Sbjct: 1 MTRKFFVGGNWKMNGDYASVDGIVTFLNASA--DNSSVDVVVAPPAPYLAYAKSKLKAGV 58
Query: 240 HVSAQNCWVRKGGAYTGEVS 299
V+AQNC+ GA+TGE+S
Sbjct: 59 LVAAQNCYKVPKGAFTGEIS 78
Score = 94 (33.1 bits), Expect = 3.9e-21, Sum P(2) = 3.9e-21
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ +LG+ WVI+GHSE F +S+ + + + A K++ CIGE LE+RE+
Sbjct: 81 MIKDLGLEWVILGHSERRHVFG-ESDALIAE-KTVHALEAGIKVVFCIGEKLEEREA 135
>gi|1351273|sp|P48492|TPIS_GRAVE TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC (TIM)
>gi|604523|gb|AAB01378.1| (L38662) triose-phosphate isomerase
[Gracilaria gracilis]
Length = 250
Frame 3 hits (HSPs): _______________
Frame 1 hits (HSPs): _____________
Annotated Domains: _________________________________________________
__________________________________________________
Database sequence: | | | | | | 250
0 50 100 150 200
__________________
Annotated Domains:
Entrez active site: BY SIMILARITY. 87
Entrez active site: BY SIMILARITY. 160
PFAM TIM: Triosephosphate isomerase 1..245
PROSITE SPASE_I_1: Signal peptidases I serine ac 228..235
PROSITE TIM: Triosephosphate isomerase active si 158..168
__________________
Plus Strand HSPs:
Score = 176 (62.0 bits), Expect = 1.0e-20, Sum P(2) = 1.0e-20
Identities = 33/72 (45%), Positives = 46/72 (63%), Frame = +3
Query: 87 NWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHVSAQNCWV 266
NWKCN + ++ ++V+ N A V+VVV+PP V+L + LR DF SAQN W+
Sbjct: 1 NWKCNLSKADIAELVSAFNAAPPIDAAHVQVVVAPPAVYLDSTRQALRADFDTSAQNAWI 60
Query: 267 RKGGAYTGEVSA 302
KGGA+TGE+ A
Sbjct: 61 SKGGAFTGELDA 72
Score = 91 (32.0 bits), Expect = 1.0e-20, Sum P(2) = 1.0e-20
Identities = 21/58 (36%), Positives = 33/58 (56%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMK-SNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE ++K S+ + + S ++ CIGE LE+RES
Sbjct: 74 MVKDVGAEWVILGHSERRHIAQLKESDHTIAMKAAYALQHASLGVIYCIGELLEERES 131
>gi|136056|sp|P00940|TPIS_CHICK TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|68424|pir||ISCHT triose-phosphate isomerase (EC 5.3.1.1) -
chicken >gi|212772|gb|AAA49094.1| (M11314) TIM [Gallus gallus]
>gi|212774|gb|AAA49095.1| (M11941) triosephosphate isomerase (EC
5.3.1.1) [Gallus gallus]
Length = 248
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 248
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 62..112
BLOCKS BL00171C: Triosephosphate isomerase prot 156..188
BLOCKS BL00171D: Triosephosphate isomerase prot 204..238
Entrez active site 95
Entrez active site 165
PFAM TIM: Triosephosphate isomerase 3..247
PRODOM PD001005: TPIS(59) TPIC(2) 3..244
PROSITE TIM: Triosephosphate isomerase active si 163..173
__________________
Plus Strand HSPs:
Score = 167 (58.8 bits), Expect = 1.3e-20, Sum P(2) = 1.3e-20
Identities = 34/78 (43%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AK+ + EVV P ++L + L V
Sbjct: 4 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSAD--TEVVCGAPSIYLDFARQKLDAKIGV 61
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 62 AAQNCYKVPKGAFTGEIS 79
Score = 99 (34.8 bits), Expect = 1.3e-20, Sum P(2) = 1.3e-20
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 82 MIKDIGAAWVILGHSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 136
Score = 41 (14.4 bits), Expect = 1.4e-14, Sum P(2) = 1.4e-14
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 112 KVAHALAEGL-GVIACIGEKLDEREAG 137
>gi|515257|pdb|1TPH|1 Chain 1, Triosephosphate Isomerase (E.C.5.3.1.1)
Complexed With Phosphoglycolohydroxamate >gi|515258|pdb|1TPH|2
Chain 2, Triosephosphate Isomerase (E.C.5.3.1.1) Complexed With
Phosphoglycolohydroxamate >gi|4558246|pdb|8TIM|A Chain A, Triose
Phosphate Isomerase >gi|4558247|pdb|8TIM|B Chain B, Triose
Phosphate Isomerase
Length = 247
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 167 (58.8 bits), Expect = 1.3e-20, Sum P(2) = 1.3e-20
Identities = 34/78 (43%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AK+ + EVV P ++L + L V
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSAD--TEVVCGAPSIYLDFARQKLDAKIGV 60
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 61 AAQNCYKVPKGAFTGEIS 78
Score = 99 (34.8 bits), Expect = 1.3e-20, Sum P(2) = 1.3e-20
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 81 MIKDIGAAWVILGHSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 135
Score = 41 (14.4 bits), Expect = 1.4e-14, Sum P(2) = 1.4e-14
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 111 KVAHALAEGL-GVIACIGEKLDEREAG 136
>gi|999971|pdb|1TPB|1 Chain 1, Triosephosphate Isomerase (E.C.5.3.1.1) Mutant
With Glu 165 Replaced By Asp (E165d) Complexed With
Phosphoglycolohydroxamate >gi|999972|pdb|1TPB|2 Chain 2,
Triosephosphate Isomerase (E.C.5.3.1.1) Mutant With Glu 165
Replaced By Asp (E165d) Complexed With Phosphoglycolohydroxamate
Length = 247
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 167 (58.8 bits), Expect = 1.3e-20, Sum P(2) = 1.3e-20
Identities = 34/78 (43%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AK+ + EVV P ++L + L V
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSAD--TEVVCGAPSIYLDFARQKLDAKIGV 60
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 61 AAQNCYKVPKGAFTGEIS 78
Score = 99 (34.8 bits), Expect = 1.3e-20, Sum P(2) = 1.3e-20
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 81 MIKDIGAAWVILGHSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 135
Score = 41 (14.4 bits), Expect = 1.4e-14, Sum P(2) = 1.4e-14
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 111 KVAHALAEGL-GVIACIGEKLDEREAG 136
>gi|6320255 ref|NP_010335.1| triosephosphate isomerase; Tpi1p
>gi|136069|sp|P00942|TPIS_YEAST TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|68428|pir||ISBYT triose-phosphate isomerase (EC 5.3.1.1) -
yeast (Saccharomyces cerevisiae) >gi|798902|emb|CAA89080.1|
(Z49209) Tpi1p [Saccharomyces cerevisiae]
>gi|1197083|gb|AAA88757.1| (J01366) triose phosphate isomerase
[Saccharomyces cerevisiae]
Length = 248
Frame 3 hits (HSPs): _________________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 248
0 50 100 150 200
Plus Strand HSPs:
Score = 181 (63.7 bits), Expect = 1.6e-20, Sum P(2) = 1.6e-20
Identities = 39/82 (47%), Positives = 55/82 (67%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLR-PD 236
M R FFVGGN+K NG+ + +K+IV LN A +P E+V EVV+ PP +L SL++ P
Sbjct: 1 MARTFFVGGNFKLNGSKQSIKEIVERLNTASIP-ENV-EVVICPPATYLDYSVSLVKKPQ 58
Query: 237 FHVSAQNCWVRKGGAYTGEVSAE 305
V AQN +++ GA+TGE S +
Sbjct: 59 VTVGAQNAYLKASGAFTGENSVD 81
Score = 84 (29.6 bits), Expect = 1.6e-20, Sum P(2) = 1.6e-20
Identities = 18/54 (33%), Positives = 33/54 (61%), Frame = +1
Query: 316 NLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
++G WVI+GHSE + F + ++F+ A + ++ CIGETLE++++
Sbjct: 85 DVGAKWVILGHSERRSYFH-EDDKFIAD-KTKFALGQGVGVILCIGETLEEKKA 136
>gi|1351275|sp|P48499|TPIS_LEIME TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|542399|pir||S42356 triose-phosphate isomerase (EC 5.3.1.1) -
Leishmania mexicana >gi|2781065|pdb|1AMK| Leishmania Mexicana
Triose Phosphate Isomerase >gi|471326|emb|CAA52804.1| (X74797)
triosephosphate isomerase [Leishmania mexicana]
Length = 251
Frame 3 hits (HSPs): ______________
Frame 1 hits (HSPs): _____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | || 251
0 50 100 150 200 250
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 5..17
BLOCKS BL00171B: Triosephosphate isomerase prot 64..114
BLOCKS BL00171C: Triosephosphate isomerase prot 160..192
BLOCKS BL00171D: Triosephosphate isomerase prot 208..242
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 3..250
Entrez active site: BY SIMILARITY. 96
Entrez active site: BY SIMILARITY. 168
PFAM TIM: Triosephosphate isomerase 5..251
PRODOM PD001005: TPIS(59) TPIC(2) 6..248
PROSITE TIM: Triosephosphate isomerase active si 166..176
__________________
Plus Strand HSPs:
Score = 168 (59.1 bits), Expect = 1.6e-20, Sum P(2) = 1.6e-20
Identities = 34/71 (47%), Positives = 50/71 (70%), Frame = +3
Query: 87 NWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLR-PDFHVSAQNCW 263
NWKCNGTT ++K+V NE + + V+ VV+P FV +P+V++ LR P + +SA+N
Sbjct: 12 NWKCNGTTASIEKLVQVFNEHTISHD--VQCVVAPTFVHIPLVQAKLRNPKYVISAENA- 68
Query: 264 VRKGGAYTGEVS 299
+ K GA+TGEVS
Sbjct: 69 IAKSGAFTGEVS 80
Score = 97 (34.1 bits), Expect = 1.6e-20, Sum P(2) = 1.6e-20
Identities = 23/59 (38%), Positives = 38/59 (64%), Frame = +1
Query: 301 LKMLVNLGIPWVIIGHSETEAAFEMKSNEFVG-K*SCLCAPARSEKLLPCIGETLEQRES 477
+ +L ++G+ WVI+GHSE + +++E V K S C + ++ CIGETL+QRE+
Sbjct: 81 MPILKDIGVHWVILGHSERRTYYG-ETDEIVAQKVSEACK--QGFMVIACIGETLQQREA 137
>gi|2266741|emb|CAA45835.1| (X64537) triosephosphate isomerase +
glyceraldehyde-3-phosphate dehydrogenase [Phytophthora infestans]
Length = 589
Frame 3 hits (HSPs): _______
Frame 2 hits (HSPs): __
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | 589
0 150 300 450
Plus Strand HSPs:
Score = 201 (70.8 bits), Expect = 1.8e-20, Sum P(2) = 1.8e-20
Identities = 42/80 (52%), Positives = 53/80 (66%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWKCNG+ + +++V LN AK+P VEVVV+P V VK+ LR V
Sbjct: 6 RKFFVGGNWKCNGSLGQAQELVGMLNTAKIPVN--VEVVVAPSQVHAATVKASLRSYVRV 63
Query: 246 SAQNCWVRKGGAYTGEVSAE 305
S Q+ W + GA+TGE SAE
Sbjct: 64 SGQDVWKQGNGAFTGETSAE 83
Score = 82 (28.9 bits), Expect = 1.8e-20, Sum P(2) = 1.8e-20
Identities = 21/58 (36%), Positives = 32/58 (55%), Frame = +1
Query: 304 KMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
+ML +LG + ++GHSE E +NE V K A + ++ CIGET + RE+
Sbjct: 83 EMLKDLGAEYTLVGHSERREKGE--TNEVVAK-KAAYALEKGLGVIACIGETKDHREA 137
Score = 37 (13.0 bits), Expect = 8.9e-16, Sum P(2) = 8.9e-16
Identities = 7/10 (70%), Positives = 9/10 (90%), Frame = +2
Query: 401 KVAYALQQGL 430
K AYAL++GL
Sbjct: 113 KAAYALEKGL 122
>gi|7160266|emb|CAB76230.1| (AL157991) triosephosphate isomerase
[Schizosaccharomyces pombe]
Length = 249
Frame 3 hits (HSPs): _________________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 249
0 50 100 150 200
Plus Strand HSPs:
Score = 177 (62.3 bits), Expect = 2.1e-20, Sum P(2) = 2.1e-20
Identities = 38/83 (45%), Positives = 53/83 (63%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGN+K NG+ E +K I+ LN K+ DV E V+ P ++L + ++ D
Sbjct: 1 MARKFFVGGNFKMNGSLESMKTIIEGLNTTKLNVGDV-ETVIFPQNMYLITTRQQVKKDI 59
Query: 240 HVSAQNCWVRKGGAYTGEVSAEN 308
V AQN + +K GAYTGE SA++
Sbjct: 60 GVGAQNVFDKKNGAYTGENSAQS 82
Score = 87 (30.6 bits), Expect = 2.1e-20, Sum P(2) = 2.1e-20
Identities = 21/56 (37%), Positives = 33/56 (58%), Frame = +1
Query: 310 LVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
L++ GI + + GHSE F+ +S+EFV A + ++ CIGETL +RE+
Sbjct: 83 LIDAGITYTLTGHSERRTIFK-ESDEFVAD-KTKFALEQGLTVVACIGETLAEREA 136
>gi|68429|pir||ISZPT triose-phosphate isomerase (EC 5.3.1.1) - fission yeast
(Schizosaccharomyces pombe)
Length = 250
Frame 3 hits (HSPs): _________________
Frame 1 hits (HSPs): ____________
Annotated Domains: _ ___
__________________________________________________
Database sequence: | | | | | | 250
0 50 100 150 200
__________________
Annotated Domains:
Entrez active site: His, Glu 95
Entrez active site: His, Glu 165
PROSITE TIM: Triosephosphate isomerase active si 163..173
__________________
Plus Strand HSPs:
Score = 177 (62.3 bits), Expect = 3.4e-20, Sum P(2) = 3.4e-20
Identities = 38/83 (45%), Positives = 53/83 (63%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGN+K NG+ E +K I+ LN K+ DV E V+ P ++L + ++ D
Sbjct: 1 MARKFFVGGNFKMNGSLESMKTIIEGLNTTKLNVGDV-ETVIFPQNMYLITTRQQVKKDI 59
Query: 240 HVSAQNCWVRKGGAYTGEVSAEN 308
V AQN + +K GAYTGE SA++
Sbjct: 60 GVGAQNVFDKKNGAYTGENSAQS 82
Score = 85 (29.9 bits), Expect = 3.4e-20, Sum P(2) = 3.4e-20
Identities = 21/56 (37%), Positives = 32/56 (57%), Frame = +1
Query: 310 LVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
L++ GI + + GHSE F+ +S+EFV A + ++ CIGETL RE+
Sbjct: 83 LIDAGITYTLTGHSERRTIFK-ESDEFVAD-KTKFALEQGLTVVACIGETLADREA 136
>gi|136053|sp|P04828|TPIS_EMENI TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|68427|pir||ISASTN triose-phosphate isomerase (EC 5.3.1.1) -
Emericella nidulans >gi|217921|dbj|BAA00908.1| (D10019)
triosephosphate isomerase [Aspergillus nidulans]
Length = 249
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 249
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 62..112
BLOCKS BL00171C: Triosephosphate isomerase prot 158..190
BLOCKS BL00171D: Triosephosphate isomerase prot 206..240
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 1..248
Entrez active site: BY SIMILARITY. 94
Entrez active site: BY SIMILARITY. 166
PFAM TIM: Triosephosphate isomerase 3..248
PRODOM PD001005: TPIS(59) TPIC(2) 3..247
PROSITE TIM: Triosephosphate isomerase active si 164..174
__________________
Plus Strand HSPs:
Score = 174 (61.3 bits), Expect = 3.4e-20, Sum P(2) = 3.4e-20
Identities = 36/82 (43%), Positives = 51/82 (62%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGN+K NG E I+ LN A + + VEVVVSPP ++L + + +
Sbjct: 1 MPRKFFVGGNFKMNGNAESTTSIIKNLNSANL--DKSVEVVVSPPALYLLQAREVANKEI 58
Query: 240 HVSAQNCWVRKGGAYTGEVSAE 305
V+AQN + + GA+TGE+S +
Sbjct: 59 GVAAQNVFDKPNGAFTGEISVQ 80
Score = 88 (31.0 bits), Expect = 3.4e-20, Sum P(2) = 3.4e-20
Identities = 20/59 (33%), Positives = 34/59 (57%), Frame = +1
Query: 301 LKMLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
++ L I W I+GHSE + +++EF+ + A +++ CIGETLE+RE+
Sbjct: 79 VQQLREANIDWTILGHSERRVILK-ETDEFIAR-KTKAAIEGGLQVIFCIGETLEEREA 135
>gi|999976|pdb|1TPC|1 Chain 1, Triosephosphate Isomerase (E.C.5.3.1.1) Mutant
With Ser 96 Replaced By Pro And Glu 165 Replaced By Asp
(S96p,E165d) Complexed With Phosphoglycolohydroxamate
>gi|999977|pdb|1TPC|2 Chain 2, Triosephosphate Isomerase
(E.C.5.3.1.1) Mutant With Ser 96 Replaced By Pro And Glu 165
Replaced By Asp (S96p,E165d) Complexed With
Phosphoglycolohydroxamate
Length = 247
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 167 (58.8 bits), Expect = 4.3e-20, Sum P(2) = 4.3e-20
Identities = 34/78 (43%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AK+ + EVV P ++L + L V
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSAD--TEVVCGAPSIYLDFARQKLDAKIGV 60
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 61 AAQNCYKVPKGAFTGEIS 78
Score = 94 (33.1 bits), Expect = 4.3e-20, Sum P(2) = 4.3e-20
Identities = 21/57 (36%), Positives = 34/57 (59%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GH E F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 81 MIKDIGAAWVILGHPERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 135
Score = 41 (14.4 bits), Expect = 1.4e-14, Sum P(2) = 1.4e-14
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 111 KVAHALAEGL-GVIACIGEKLDEREAG 136
>gi|1064977|pdb|1TPW|A Chain A, Triosephosphate Isomerase (E.C.5.3.1.1) Mutant
With Ser 96 Replaced By Pro (S96p) Complexed With
Phosphoglycolohydroxamate >gi|1064978|pdb|1TPW|B Chain B,
Triosephosphate Isomerase (E.C.5.3.1.1) Mutant With Ser 96 Replaced
By Pro (S96p) Complexed With Phosphoglycolohydroxamate
Length = 247
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 167 (58.8 bits), Expect = 4.3e-20, Sum P(2) = 4.3e-20
Identities = 34/78 (43%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AK+ + EVV P ++L + L V
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSAD--TEVVCGAPSIYLDFARQKLDAKIGV 60
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 61 AAQNCYKVPKGAFTGEIS 78
Score = 94 (33.1 bits), Expect = 4.3e-20, Sum P(2) = 4.3e-20
Identities = 21/57 (36%), Positives = 34/57 (59%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GH E F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 81 MIKDIGAAWVILGHPERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 135
Score = 41 (14.4 bits), Expect = 1.4e-14, Sum P(2) = 1.4e-14
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 111 KVAHALAEGL-GVIACIGEKLDEREAG 136
>gi|230359|pdb|1TIM|A Chain A, Triose Phosphate Isomerase (E.C.5.3.1.1)
>gi|230360|pdb|1TIM|B Chain B, Triose Phosphate Isomerase
(E.C.5.3.1.1)
Length = 247
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 162 (57.0 bits), Expect = 4.3e-20, Sum P(2) = 4.3e-20
Identities = 33/78 (42%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TL+ AK+ + EVV P ++L + L V
Sbjct: 3 RKFFVGGNWKMNGKRKSLGELIHTLDGAKLSAD--TEVVCGAPSIYLDFARQKLDAKIGV 60
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 61 AAQNCYKVPKGAFTGEIS 78
Score = 99 (34.8 bits), Expect = 4.3e-20, Sum P(2) = 4.3e-20
Identities = 22/57 (38%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+GHSE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 81 MIKDIGAAWVILGHSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 135
Score = 41 (14.4 bits), Expect = 4.7e-14, Sum P(2) = 4.7e-14
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 111 KVAHALAEGL-GVIACIGEKLDEREAG 136
>gi|230405|pdb|1YPI|A Chain A, Triose Phosphate Isomerase (TIM) (E.C.5.3.1.1)
>gi|230406|pdb|1YPI|B Chain B, Triose Phosphate Isomerase (TIM)
(E.C.5.3.1.1) >gi|230791|pdb|2YPI|A Chain A, Triose Phosphate
Isomerase (TIM) (E.C.5.3.1.1) Complex With 2-Phosphoglycolic Acid
(PGA) >gi|230792|pdb|2YPI|B Chain B, Triose Phosphate Isomerase
(TIM) (E.C.5.3.1.1) Complex With 2-Phosphoglycolic Acid (PGA)
>gi|443550|pdb|7TIM|A Chain A, Triosephosphate Isomerase
(E.C.5.3.1.1) Complex With Phosphoglycolohydroxamate
>gi|443551|pdb|7TIM|B Chain B, Triosephosphate Isomerase
(E.C.5.3.1.1) Complex With Phosphoglycolohydroxamate
Length = 247
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 176 (62.0 bits), Expect = 5.4e-20, Sum P(2) = 5.4e-20
Identities = 38/80 (47%), Positives = 54/80 (67%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLR-PDFH 242
R FFVGGN+K NG+ + +K+IV LN A +P E+V EVV+ PP +L SL++ P
Sbjct: 2 RTFFVGGNFKLNGSKQSIKEIVERLNTASIP-ENV-EVVICPPATYLDYSVSLVKKPQVT 59
Query: 243 VSAQNCWVRKGGAYTGEVSAE 305
V AQN +++ GA+TGE S +
Sbjct: 60 VGAQNAYLKASGAFTGENSVD 80
Score = 84 (29.6 bits), Expect = 5.4e-20, Sum P(2) = 5.4e-20
Identities = 18/54 (33%), Positives = 33/54 (61%), Frame = +1
Query: 316 NLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
++G WVI+GHSE + F + ++F+ A + ++ CIGETLE++++
Sbjct: 84 DVGAKWVILGHSERRSYFH-EDDKFIAD-KTKFALGQGVGVILCIGETLEEKKA 135
>gi|6841084|gb|AAF28895.1|AF124845_1 (AF124845) triose phosphate isomerase
[Candida albicans]
Length = 248
Frame 3 hits (HSPs): ___________________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 248
0 50 100 150 200
Plus Strand HSPs:
Score = 171 (60.2 bits), Expect = 6.9e-20, Sum P(2) = 6.9e-20
Identities = 39/97 (40%), Positives = 59/97 (60%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPV-VKSLLRPD 236
M R+FFVGGN+K NGT +++ I+ LN+A +P + VEVV+ PP ++L + V+ +P
Sbjct: 1 MARQFFVGGNFKANGTKQQITSIIDNLNKADLPKD--VEVVICPPALYLGLAVEQNKQPT 58
Query: 237 FHVSAQNCWVRKGGAYTGEVSAENAC*FGNSLGYYWSL 350
+ AQN + + GA+TGE A G S W+L
Sbjct: 59 VAIGAQNVFDKSCGAFTGETCASQILDVGAS----WTL 92
Score = 88 (31.0 bits), Expect = 6.9e-20, Sum P(2) = 6.9e-20
Identities = 19/55 (34%), Positives = 33/55 (60%), Frame = +1
Query: 310 LVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRE 474
++++G W + GHSE + +S+EF+ + A K++ CIGETLE+R+
Sbjct: 83 ILDVGASWTLTGHSERRTIIK-ESDEFIAE-KTKFALDTGVKVILCIGETLEERK 135
>gi|7270988|emb|CAB77631.1| (AJ390491) triosephosphate isomerase [Candida
albicans]
Length = 248
Frame 3 hits (HSPs): ___________________
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 248
0 50 100 150 200
Plus Strand HSPs:
Score = 171 (60.2 bits), Expect = 6.9e-20, Sum P(2) = 6.9e-20
Identities = 39/97 (40%), Positives = 59/97 (60%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPV-VKSLLRPD 236
M R+FFVGGN+K NGT +++ I+ LN+A +P + VEVV+ PP ++L + V+ +P
Sbjct: 1 MARQFFVGGNFKANGTKQQITSIIDNLNKADLPKD--VEVVICPPALYLGLAVEQNKQPT 58
Query: 237 FHVSAQNCWVRKGGAYTGEVSAENAC*FGNSLGYYWSL 350
+ AQN + + GA+TGE A G S W+L
Sbjct: 59 VAIGAQNVFDKSCGAFTGETCASQILDVGAS----WTL 92
Score = 88 (31.0 bits), Expect = 6.9e-20, Sum P(2) = 6.9e-20
Identities = 19/55 (34%), Positives = 33/55 (60%), Frame = +1
Query: 310 LVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRE 474
++++G W + GHSE + +S+EF+ + A K++ CIGETLE+R+
Sbjct: 83 ILDVGASWTLTGHSERRTIIK-ESDEFIAE-KTKFALDTGVKVILCIGETLEERK 135
>gi|1064969|pdb|1TPU|A Chain A, Triosephosphate Isomerase (E.C.5.3.1.1) Mutant
With His 95 Replaced By Asn (H95n) Complexed With
Phosphoglycolohydroxamate >gi|1064970|pdb|1TPU|B Chain B,
Triosephosphate Isomerase (E.C.5.3.1.1) Mutant With His 95 Replaced
By Asn (H95n) Complexed With Phosphoglycolohydroxamate
Length = 247
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
Plus Strand HSPs:
Score = 167 (58.8 bits), Expect = 6.9e-20, Sum P(2) = 6.9e-20
Identities = 34/78 (43%), Positives = 48/78 (61%), Frame = +3
Query: 66 RKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDFHV 245
RKFFVGGNWK NG + + +++ TLN AK+ + EVV P ++L + L V
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSAD--TEVVCGAPSIYLDFARQKLDAKIGV 60
Query: 246 SAQNCWVRKGGAYTGEVS 299
+AQNC+ GA+TGE+S
Sbjct: 61 AAQNCYKVPKGAFTGEIS 78
Score = 92 (32.4 bits), Expect = 6.9e-20, Sum P(2) = 6.9e-20
Identities = 21/57 (36%), Positives = 35/57 (61%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
M+ ++G WVI+G+SE F +S+E +G+ A A ++ CIGE L++RE+
Sbjct: 81 MIKDIGAAWVILGNSERRHVFG-ESDELIGQ-KVAHALAEGLGVIACIGEKLDEREA 135
Score = 41 (14.4 bits), Expect = 1.4e-14, Sum P(2) = 1.4e-14
Identities = 11/27 (40%), Positives = 17/27 (62%), Frame = +2
Query: 401 KVAYALQQGLKSYYHALGKLLNSVKAG 481
KVA+AL +GL +G+ L+ +AG
Sbjct: 111 KVAHALAEGL-GVIACIGEKLDEREAG 136
>gi|267155|sp|P30741|TPIS_CULTA TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|156691|gb|AAA73976.1| (L07390) triosephosphate isomerase [Culex
tarsalis]
Length = 247
Frame 3 hits (HSPs): ________________
Frame 2 hits (HSPs): ___________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 2..14
BLOCKS BL00171B: Triosephosphate isomerase prot 61..111
BLOCKS BL00171C: Triosephosphate isomerase prot 155..187
BLOCKS BL00171D: Triosephosphate isomerase prot 203..237
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 1..245
Entrez active site: BY SIMILARITY. 94
Entrez active site: BY SIMILARITY. 164
PFAM TIM: Triosephosphate isomerase 2..246
PRODOM PD001005: TPIS(59) TPIC(2) 2..245
PROSITE TIM: Triosephosphate isomerase active si 162..172
__________________
Plus Strand HSPs:
Score = 148 (52.1 bits), Expect = 1.3e-19, Sum P(2) = 1.3e-19
Identities = 33/80 (41%), Positives = 44/80 (55%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
MGRKF VGGNWK NG + + L + + EVVV P +L + +S L
Sbjct: 1 MGRKFCVGGNWKMNGDKASIADLCKVLTTGPLNAD--TEVVVGCPAPYLTLARSQLPDSV 58
Query: 240 HVSAQNCWVRKGGAYTGEVS 299
V+AQNC+ GA+TGE+S
Sbjct: 59 CVAAQNCYKVPKGAFTGEIS 78
Score = 111 (39.1 bits), Expect = 1.3e-19, Sum P(2) = 1.3e-19
Identities = 26/57 (45%), Positives = 36/57 (63%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
ML +L I WVI+GHSE A F +S+E + A A K++ CIGETL++RE+
Sbjct: 81 MLKDLNIGWVILGHSERRAIFG-ESDELIAD-KVAHALAEGLKVIACIGETLQEREA 135
Score = 49 (17.2 bits), Expect = 3.7e-13, Sum P(2) = 3.7e-13
Identities = 15/51 (29%), Positives = 27/51 (52%), Frame = +2
Query: 329 LGLLLVTLKRRQLLK*NQMSLWENKVAYALQQGLKSYYHALGKLLNSVKAG 481
+G +++ R+ + L +KVA+AL +GLK +G+ L +AG
Sbjct: 87 IGWVILGHSERRAIFGESDELIADKVAHALAEGLK-VIACIGETLQEREAG 136
>gi|1174748|sp|P07669|TPIS_SCHPO TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|173515|gb|AAA35348.1| (M14432) triose-phosphate-isomerase
[Schizosaccharomyces pombe]
Length = 250
Frame 3 hits (HSPs): _________________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 250
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 2..14
BLOCKS BL00171B: Triosephosphate isomerase prot 62..112
BLOCKS BL00171C: Triosephosphate isomerase prot 156..188
BLOCKS BL00171D: Triosephosphate isomerase prot 204..238
DOMO DM00400: TRIOSEPHOSPHATEISOMERASE 1..248
Entrez active site: BY SIMILARITY. 95
Entrez active site: BY SIMILARITY. 165
PFAM TIM: Triosephosphate isomerase 2..248
PRODOM PD001005: TPIS(59) TPIC(2) 2..244
PROSITE TIM: Triosephosphate isomerase active si 163..173
__________________
Plus Strand HSPs:
Score = 177 (62.3 bits), Expect = 1.4e-19, Sum P(2) = 1.4e-19
Identities = 38/83 (45%), Positives = 53/83 (63%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKFFVGGN+K NG+ E +K I+ LN K+ DV E V+ P ++L + ++ D
Sbjct: 1 MARKFFVGGNFKMNGSLESMKTIIEGLNTTKLNVGDV-ETVIFPQNMYLITTRQQVKKDI 59
Query: 240 HVSAQNCWVRKGGAYTGEVSAEN 308
V AQN + +K GAYTGE SA++
Sbjct: 60 GVGAQNVFDKKNGAYTGENSAQS 82
Score = 79 (27.8 bits), Expect = 1.4e-19, Sum P(2) = 1.4e-19
Identities = 20/56 (35%), Positives = 31/56 (55%), Frame = +1
Query: 310 LVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
L++ I + + GHSE F+ +S+EFV A + ++ CIGETL RE+
Sbjct: 83 LIDAAITYTLTGHSERRTIFK-ESDEFVAD-KTKFALEQGLTVVACIGETLADREA 136
>gi|2143243|emb|CAA73817.1| (Y13387) triosephosphate isomerase [Entamoeba
histolytica]
Length = 261
Frame 3 hits (HSPs): _________________
Frame 1 hits (HSPs): ___________
__________________________________________________
Database sequence: | | | | | | | 261
0 50 100 150 200 250
Plus Strand HSPs:
Score = 164 (57.7 bits), Expect = 1.8e-19, Sum P(2) = 1.8e-19
Identities = 39/85 (45%), Positives = 52/85 (61%), Frame = +3
Query: 60 MGR-KFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGE--DVVEVVVSPPFVFLPVVKSLLR 230
MG KF VGGNWKCNGT ++ + + A V E VEV+V PF+++P V+ +L
Sbjct: 1 MGAGKFVVGGNWKCNGTLASIETLTKGV-AASVDAELAKKVEVIVGVPFIYIPKVQQILA 59
Query: 231 PDFH-----VSAQNCWVRKGGAYTGEV 296
+ + VSA+N W K GAYTGEV
Sbjct: 60 GEANGANILVSAENAWT-KSGAYTGEV 85
Score = 92 (32.4 bits), Expect = 1.8e-19, Sum P(2) = 1.8e-19
Identities = 24/55 (43%), Positives = 33/55 (60%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQR 471
MLV+ +P+VI+GHSE F +SNE V + + A K++ CIGET QR
Sbjct: 89 MLVDCQVPYVILGHSERRQIFH-ESNEQVAEKVKVAIDA-GLKVIACIGETEAQR 141
>gi|267156|sp|P29613|TPIS_DROME TRIOSEPHOSPHATE ISOMERASE (TIM)
>gi|103429|pir||S18604 triose-phosphate isomerase (EC 5.3.1.1) -
fruit fly (Drosophila melanogaster) >gi|10946|emb|CAA40804.1|
(X57576) triosephosphate isomerase [Drosophila melanogaster]
Length = 247
Frame 3 hits (HSPs): ________________
Frame 1 hits (HSPs): ____________
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | 247
0 50 100 150 200
__________________
Annotated Domains:
BLOCKS BL00171A: Triosephosphate isomerase prot 3..15
BLOCKS BL00171B: Triosephosphate isomerase prot 62..112
BLOCKS BL00171C: Triosephosphate isomerase prot 156..188
BLOCKS BL00171D: Triosephosphate isomerase prot 204..238
Entrez active site: BY SIMILARITY. 94
Entrez active site: BY SIMILARITY. 164
PFAM TIM: Triosephosphate isomerase 3..247
PRODOM PD001005: TPIS(59) TPIC(2) 3..246
PROSITE TIM: Triosephosphate isomerase active si 162..172
__________________
Plus Strand HSPs:
Score = 148 (52.1 bits), Expect = 2.1e-19, Sum P(2) = 2.1e-19
Identities = 31/80 (38%), Positives = 48/80 (60%), Frame = +3
Query: 60 MGRKFFVGGNWKCNGTTEEVKKIVTTLNEAKVPGEDVVEVVVSPPFVFLPVVKSLLRPDF 239
M RKF VGGNWK NG + + +I TL+ A + + EVV+ P ++L ++LL +
Sbjct: 1 MSRKFCVGGNWKMNGDQKSIAEIAKTLSSAAL--DPNTEVVIGCPAIYLMYARNLLPCEL 58
Query: 240 HVSAQNCWVRKGGAYTGEVS 299
++ QN + GA+TGE+S
Sbjct: 59 GLAGQNAYKVAKGAFTGEIS 78
Score = 109 (38.4 bits), Expect = 2.1e-19, Sum P(2) = 2.1e-19
Identities = 25/57 (43%), Positives = 36/57 (63%), Frame = +1
Query: 307 MLVNLGIPWVIIGHSETEAAFEMKSNEFVGK*SCLCAPARSEKLLPCIGETLEQRES 477
ML ++G WVI+GHSE A F +S+ + + A A K++ CIGETLE+RE+
Sbjct: 81 MLKDIGADWVILGHSERRAIFG-ESDALIAE-KAEHALAEGLKVIACIGETLEEREA 135
WARNING: HSPs involving 127 database sequences were not reported due to the
limiting value of parameter B = 50.
Parameters:
filter=none
matrix=BLOSUM62
V=50
B=50
E=10
gi
H=1
sort_by_pvalue
echofilter
ctxfactor=5.97
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
Std. 0 BLOSUM62 0.318 0.135 0.401
+3 0 BLOSUM62 0.318 0.135 0.401 0.344 0.152 0.532
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+2 0 BLOSUM62 0.318 0.135 0.401 0.352 0.157 0.623
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+1 0 BLOSUM62 0.318 0.135 0.401 0.345 0.148 0.472
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-1 0 BLOSUM62 0.318 0.135 0.401 0.336 0.144 0.464
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-2 0 BLOSUM62 0.318 0.135 0.401 0.360 0.158 0.632
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-3 0 BLOSUM62 0.318 0.135 0.401 0.323 0.135 0.389
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+3 0 159 159 10. 74 3 12 22 0.099 34
30 0.098 37
+2 0 160 159 10. 74 3 12 22 0.099 34
30 0.098 37
+1 0 160 159 10. 74 3 12 22 0.099 34
30 0.098 37
-1 0 160 160 10. 74 3 12 22 0.10 34
30 0.099 37
-2 0 160 159 10. 74 3 12 22 0.099 34
30 0.098 37
-3 0 159 159 10. 74 3 12 22 0.099 34
30 0.098 37
Statistics:
Database: /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
Title: nr
Release date: unknown
Posted date: 8:50 PM CDT May 27, 2000
Format: BLAST
# of letters in database: 158,518,215
# of sequences in database: 505,245
# of database sequences satisfying E: 177
No. of states in DFA: 591 (58 KB)
Total size of DFA: 198 KB (256 KB)
Time to generate neighborhood: 0.01u 0.01s 0.02t Elapsed: 00:00:00
No. of threads or processors used: 4
Search cpu time: 221.05u 1.09s 222.14t Elapsed: 00:01:42
Total cpu time: 221.18u 1.15s 222.33t Elapsed: 00:01:42
Start: Wed Feb 14 12:43:30 2001 End: Wed Feb 14 12:45:12 2001
WARNINGS ISSUED: 2
Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000