James E.T. Moncur, Principal Investigator; Roger S. Fujioka, Unknown, Barbers Point Biological & Sediment Monitoring, Oahu, HI, 1986-1998, (NODC Accession 9900098): Water Resources Research Center, University of Hawaii at Manoa, Honolulu, Hawaii.
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Australian Opheliidae (Polychaeta) DELTA database (<http://www.museum.vic.gov.au/poly/ophitems.html>);
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Systema Naturae 2000 / Classification (<http://sn2000.taxonomy.nl/Main/Classification/..%5C..%5C..%5CMain%5CClassification%5C15265.htm>);
Smithsonian Marine Station at Fort Pierce (<http://www.sms.si.edu/IRLSpec/aspecies2.htm>);
Cephalaspidea from around the world (<http://www.medslugs.de/Opi/CEPHALASPIDEA.htm>):
Sea slug forum (<http://www.seaslugforum.net/species.htm>);
A Biotic Database of Indo-Pacific Marine Mollusks (<http://data.acnatsci.org/obis/>);
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Contributor: Dr. Phillip Moravcik; Originators: James E.T. Moncur, Roger S. Fujioka, William J. Cooke, E. Alison Kay, Richard C. Swartz, and Ross S. Tanimoto
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To assess benthic environmental quality in region of sewer outfall.
INSTRUMENT TYPES: A van Veen grab sampler deployed from a stern-mounted A-frame on the research vessel Noi I Kai was used to obtain bottom samples at all seven stations. SAMPLING METHODOLOGY: The sampling methodology used in this study generally followed the recommendations of Swartz (1978) and US EPA guidelines (EPA 1987a, 1987b), hereafter refered to as EPA procedures. A modified 0.16-m2 van Veen grab sampler deployed from a stern-mounted A-frame on the research vessel Noi I Kai was used to obtain bottom samples at all seven stations. Penetration of the sampler was adequate for all replicates. The minimum penetration depth for all grabs was 8 cm and the maximum was 15 cm. Five van Veen grabs were taken at each station. A subsample 7.6 cm in diameter by 5 cm deep was taken from each grab sample for infaunal analysis and a subsample 4.8 cm in diameter by 5 cm deep for mollusk analysis. Subsampling was necessary because the epifauna and infauna in the area are known to be both small and abundant and processing of the entire sample would be impractical. Replicated grab samples at each station, rather than replicated subsamples from one grab sample, were used to provide information on intrastation variability. All six nonmollusk subcores were processed on a 0.5-mm screen. Samples for geochemical analyses (total organic carbon (TOC), oxidation-reduction potential (ORP), sediment oil and grease (O&G), total Kjeldahl nitrogen (TKN), and grain size) were obtained from the grabs from which the biological subcores were taken. Each replicate van Veen grab contained more than enough sediment for both purposes (methods HI0020117). Three subsamples 7.6 cm in diameter by 5 cm deep (one from each of three different grab samples) were taken from all stations. The top 2 cm of sediment of each subsample were used for geochemical analysis. Samples for TOC and sediment O&G analysis were put in screw-cap jars, placed on ice, and taken to the laboratory for analysis. Sediment analyses of sediment grain size, ORP, TKN, and O&G followed EPA procedures. Analysis of TOC was carried out by PACE, Inc. Laboratory (Southern California).
Reference:
Sample Processing: Handling and processing of biological samples followed EPA procedures. Nonmollusk samples were fixed with buffered 10% formalin for a minimum of 24 hours. The fixed samples were elutriated using the technique of Saunders et al. (1965). This method successfully removes from the sediment all organisms that are not heavily calcified. Samples were washed six times, and the water from each was poured through 0.5 mm mesh sieves. Polychaetes and other invertebrates retained on the sieve were transferred to alcohol, stained with rose bengal solution, and stored in 70% ethanol. The mollusk samples were fixed in 75% alcohol for 24 hours, decanted, fixed again for another 24 hours, then air dried. Aliquot portions (15cm3) were then sorted following the methods of Kay (1980) and Kay and Kawamoto (1983). When large carbonate rubble fragments were collected in the samples, the rubble fragments were carefully washed and visually examined to ensure that any organisms on the external surfaces were removed. The fragments were then placed in a nitric acid bath for 24 hours to dissolve the carbonate and to recover organisms living in burrows. The acid dissolution technique used was modified from the methods of Brock and Brock (1977), as described in Nelson (1986). Because the biological subcores had to be processed using two different procedures, one for mollusks and the other for all other organisms, the components of the fauna were not directly comparable and thus were analyzed separately. Because the mollusks were not separated into living and dead shell fractions, they represent time-averaged samples. All specimens were identified to the lowest taxonomic level possible. Voucher specimens were submitted to taxonomic specialists for verification when necessary.
Reference:
see methodolgy section in originator data and this metadata record
not aplicable
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NODC Accession #9900098
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| Data format: | Subdirectories: gr_size/, mol_spec/, nodcmeta/, nonmol/, sed_chem/; one sampling per year from 1986 to 1998, some data excluding years 1987, 1988, 1989, and 1990 in format XLS (version 5.0) Microsoft Excel (version 5.0) with ASCII files of each sheet within each Excel file Size: 2690000 |
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