RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Sockeye Salmon ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Oncorhynchus nerka
FDA Seafood List Common Name: Sockeye Salmon
FDA Seafood List Market Name: Salmon, Sockeye (Red, Blueback)
Unique IEF Gel Code: 19940907gel04
Unique Web Code: syg02Srr
Tissue Aliquot Code: onconerk H94-003 #10m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c Co-Migrated	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	38	3.4	0.294022	16	7.0334	18.6	15.3	3.5	37.9	15.6	0.25407
2	4.55	187	16.6	0.383856	5	3.05024	8.1	6.6	4.55	187.5	4.9	0.348936
3	5.2	267	23.6	0.529673	4	3.94511	10.4	8.6	5.2	266.7	4.3	0.516415
4	5.85	418	37	0.673519	3	3.86256	10.2	8.4	5.85	418.4	3.1	0.693058
5	6.55	588	52.1	0.505722	4	3.69863	9.8	8	6.55	587.7	4.4	0.475716
6	6.85	628	55.6	0.214629	6	2.21263	5.9	4.8	6.85	628.1	6.5	0.19217
7	7.35	721	63.9	0.522146	5	4.55759	12.1	9.9	7.35	719.7	5.1	0.506401
8	8.15	881	78	0.0963134	12	1.85938	4.9	4	8.15	879	11.5	0.0911667
9	8.45	944	83.6	0.118479	8	1.60032	4.2	3.5	8.45	943.5	7.8	0.116111
10	8.65	984	87.2	0.126296	6	1.27823	3.4	2.8	8.65	983.8	6	0.120128
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1117	98.9	0.482564	6	4.69862	12.4	10.2	9.3	1116.3	5.6	0.474868

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.682	206	18.3	0.0995834	7	1.05398	1.4	1.3	4.682	207.5	7	0.0850923
2	4.909	231	20.5	0.0686276	7	0.751146	1	0.9	4.909	233.4	6.5	0.0650354
3	5.073	249	22.1	0.0326492	2	0.115669	0.2	0.1	5.073	249.1	2.2	0.029509
4	5.182	261	23.2	0.161674	6	1.62246	2.2	1.9	5.182	262.3	6.3	0.146447
5	5.287	284	25.2	0.0510269	18	1.49225	2	1.8	5.287	276.8	17.8	0.0473963
6	5.35	299	26.6	0.0614371	3	0.223374	0.3	0.3	5.35	299.4	3	0.0416898
7	5.379	306	27.2	0.233197	3	1.15739	1.6	1.4	5.379	306	3.1	0.213708
8	5.504	336	29.9	0.27598	4	1.78817	2.4	2.1	5.504	335.6	3.6	0.278318
9	5.537	344	30.6	0.318625	4	1.98365	2.7	2.4	5.537	344.6	3.6	0.312921
10	5.654	372	33.1	0.234353	4	1.66207	2.2	2	5.654	371.8	4.2	0.2229
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.704	384	34.1	0.11336	2	0.408607	0.6	0.5	5.704	384.6	2.1	0.11137
12	6.008	456	40.5	0.626398	2	2.65835	3.6	3.2	6.008	455.9	2.4	0.619206
13	6.103	478	42.5	0.313062	3	1.42315	1.9	1.7	6.103	477.7	2.6	0.308174
14	6.158	491	43.6	0.554303	2	2.34436	3.2	2.8	6.158	491.5	2.4	0.551037
15	6.295	523	46.5	0.249783	3	1.32317	1.8	1.6	6.295	523	3.4	0.220559
16	6.454	560	49.8	0.201665	4	1.25199	1.7	1.5	6.454	560	3.9	0.180979
17	6.509	573	50.9	0.594804	4	4.20552	5.7	5	6.509	572.9	4.3	0.556827
18	6.548	582	51.7	0.230844	2	0.751801	1	0.9	6.548	581.5	1.5	0.275425
19	6.595	589	52.4	0.467276	2	1.43388	1.9	1.7	6.595	588.7	1.7	0.478649
20	6.665	599	53.2	0.266956	5	2.39149	3.2	2.9	6.665	599.6	4.7	0.284606
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.707	605	53.8	0.476172	2	1.36994	1.8	1.6	6.707	605.5	1.7	0.449015
22	7.248	698	62	0.206496	4	1.52646	2.1	1.8	7.248	697.8	4.3	0.201718
23	7.378	722	64.2	0.490873	4	3.11726	4.2	3.7	7.378	721.5	3.5	0.498457
24	7.506	747	66.4	0.440136	4	2.78753	3.8	3.3	7.506	746.8	3.7	0.42224
25	7.686	782	69.5	0.0998911	4	0.69744	0.9	0.8	7.686	782.4	4.4	0.0897193
26	7.768	798	70.9	0.408881	5	3.37533	4.6	4	7.768	797.6	4.8	0.392734
27	8.497	948	84.3	0.237192	15	5.18262	7	6.2	8.497	942.4	15.1	0.193384
28	8.632	978	86.9	0.432807	22	14.7398	19.9	17.6	8.632	977.7	21.6	0.384432
29	8.863	1025	91.1	0.364464	11	6.53761	8.8	7.8	8.863	1021.1	10.9	0.339672
30	9.097	1072	95.3	0.277046	10	4.77561	6.4	5.7	9.097	1066.5	10.2	0.264923

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	39	3.5	0.246772	10	4.35365	5.6	5	3.5	40.9	10.1	0.24327
2	4.55	198	17.6	0.370372	6	3.00459	3.8	3.5	4.55	198.5	5.6	0.304449
3	4.88	230	20.4	0.0600017	3	0.309733	0.4	0.4	4.88	230	2.9	0.0592971
4	4.973	239	21.2	0.0402473	3	0.19298	0.2	0.2	4.973	239.4	2.7	0.0396046
5	5.066	248	22	0.0834406	3	0.44396	0.6	0.5	5.066	247.9	3.1	0.0814546
6	5.2	261	23.2	0.175739	8	2.24207	2.9	2.6	5.2	263.5	8.2	0.154925
7	5.281	281	25	0.349883	4	2.61934	3.3	3	5.281	280.7	4.4	0.337241
8	5.382	306	27.2	0.218751	3	1.19936	1.5	1.4	5.382	305.9	3.4	0.196395
9	5.507	337	30	0.219829	4	1.605	2.1	1.9	5.507	336.8	4.1	0.220883
10	5.539	345	30.7	0.288945	3	1.60515	2.1	1.9	5.539	345.4	3.2	0.28281
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.6	360	32	0.126223	6	1.22127	1.6	1.4	5.6	360.8	6.3	0.109895
12	5.656	374	33.2	0.191472	4	1.31399	1.7	1.5	5.656	374.2	3.8	0.193673
13	5.709	387	34.4	0.109216	2	0.482569	0.6	0.6	5.709	386.5	2.4	0.114131
14	5.85	422	37.5	0.59693	3	3.16704	4	3.7	5.85	422.6	2.9	0.618412
15	6.015	459	40.8	0.532246	3	2.56236	3.3	3	6.015	459.6	2.8	0.521744
16	6.113	481	42.8	0.280458	3	1.35037	1.7	1.6	6.113	481.2	2.9	0.261803
17	6.175	495	44	0.476528	2	2.00259	2.6	2.3	6.175	495.4	2.4	0.478108
18	6.323	528	46.9	0.216066	3	1.10903	1.4	1.3	6.323	528	3.3	0.19123
19	6.492	566	50.3	0.156936	4	1.07522	1.4	1.2	6.492	566.1	4.3	0.142378
20	6.55	579	51.5	0.567448	5	4.33844	5.5	5	6.55	579.5	4.7	0.518482
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.615	589	52.4	0.194192	2	0.75346	1	0.9	6.615	588.1	1.7	0.249993
22	6.661	596	53	0.491654	1	1.3139	1.7	1.5	6.661	595.7	1.5	0.495875
23	6.726	606	53.9	0.294805	4	1.95538	2.5	2.3	6.726	605.6	3.7	0.294439
24	6.772	613	54.5	0.454805	2	1.3432	1.7	1.6	6.772	612.9	1.7	0.444148
25	6.85	625	55.6	0.186668	3	1.04303	1.3	1.2	6.85	624.3	3.2	0.184461
26	7.165	681	60.5	0.574443	2	2.24872	2.9	2.6	7.165	681	2.3	0.555413
27	7.35	714	63.5	0.432343	6	4.23055	5.4	4.9	7.35	713.1	6	0.395437
28	7.424	728	64.7	0.420657	3	2.5049	3.2	2.9	7.424	727.5	3.3	0.42369
29	7.603	762	67.7	0.424325	4	3.08534	3.9	3.6	7.603	761.3	4.2	0.410526
30	7.745	789	70.1	0.0780544	3	0.464155	0.6	0.5	7.745	788.4	3.4	0.0774094
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.882	815	72.4	0.327926	5	3.03229	3.9	3.5	7.882	815.2	5.4	0.315537
32	8.15	866	77	0.332056	6	2.70663	3.5	3.1	8.15	866.9	5.6	0.273247
33	8.45	933	82.9	0.0655005	7	0.722475	0.9	0.8	8.45	931	7.2	0.0568832
34	8.532	953	84.7	0.0907239	13	1.99141	2.5	2.3	8.532	951.3	13	0.0866228
35	8.65	982	87.3	0.225884	12	4.76773	6.1	5.5	8.65	979.2	11.6	0.231227
36	8.862	1024	91	0.3106	7	3.91202	5	4.5	8.862	1021.9	7.2	0.306421
37	9.103	1072	95.3	0.184487	10	3.07396	3.9	3.6	9.103	1067.4	9.5	0.181721
38	9.3	1111	98.8	0.151361	11	2.88787	3.7	3.3	9.3	1104.2	11.4	0.143048

PeakCounter: 38

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	32	2.8	0.240636	23	9.42964	21.7	18.3	3.5	25.9	23.3	0.228123
2	4.55	186	16.5	0.469433	4	3.21855	7.4	6.3	4.55	186.1	4.4	0.409815
3	5.2	268	23.8	0.585529	4	4.164	9.6	8.1	5.2	267.9	4.1	0.572447
4	5.85	411	36.5	0.702917	3	4.01889	9.2	7.8	5.85	411.2	3.2	0.711955
5	6.55	587	52.2	0.565682	4	4.06529	9.3	7.9	6.55	586.2	4.2	0.545596
6	6.85	625	55.6	0.261885	6	2.60249	6	5.1	6.85	625	6.4	0.229345
7	7.35	715	63.6	0.551244	5	4.74425	10.9	9.2	7.35	714.5	5	0.540043
8	8.15	871	77.4	0.0969752	11	1.86723	4.3	3.6	8.15	870.1	11.2	0.0938277
9	8.45	936	83.2	0.104736	8	1.42486	3.3	2.8	8.45	936.7	8.2	0.097976
10	8.65	978	86.9	0.120103	6	1.15556	2.7	2.2	8.65	977.7	5.7	0.114781
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1112	98.8	0.65117	6	6.7989	15.6	13.2	9.3	1110.9	6.2	0.619224

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.656	196	17.4	0.0817384	5	0.672319	1.1	1	4.656	196.3	5.4	0.0698257
2	4.859	221	19.7	0.092312	4	0.6422	1.1	0.9	4.859	220.6	4.1	0.0887421
3	4.924	229	20.4	0.0782077	3	0.473453	0.8	0.7	4.924	228.4	3.4	0.078485
4	4.989	237	21.1	0.0656835	3	0.329442	0.5	0.5	4.989	236.5	3	0.0618861
5	5.102	251	22.3	0.188528	6	1.69954	2.8	2.5	5.102	252.1	5.9	0.163484
6	5.246	273	24.3	0.0454188	18	1.22266	2	1.8	5.246	264.6	18	0.0383237
7	5.297	284	25.3	0.0314056	5	0.368176	0.6	0.5	5.297	290.4	5.1	0.0409575
8	5.353	296	26.3	0.21366	2	0.811954	1.3	1.2	5.353	296.2	2.4	0.188927
9	5.52	332	29.5	0.214057	3	1.14657	1.9	1.7	5.52	332.4	3	0.214426
10	5.548	338	30.1	0.353953	3	1.7429	2.9	2.5	5.548	338.3	2.8	0.34717
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.613	352	31.3	0.107848	6	1.05235	1.7	1.5	5.613	352.6	6.4	0.0928252
12	5.683	367	32.7	0.281966	4	1.83298	3	2.6	5.683	366.1	3.8	0.275106
13	5.799	392	34.9	0.0780308	3	0.430341	0.7	0.6	5.799	391.3	3.2	0.0760717
14	5.881	411	36.6	0.0577226	5	0.422105	0.7	0.6	5.881	411.7	5.1	0.0466622
15	6.039	452	40.2	0.4841	2	1.95665	3.3	2.8	6.039	452.3	2.3	0.478843
16	6.108	470	41.8	0.395507	2	1.60305	2.7	2.3	6.108	470.1	2.3	0.3866
17	6.166	485	43.1	0.447061	2	1.6708	2.8	2.4	6.166	485.4	2.1	0.451245
18	6.301	520	46.3	0.293415	3	1.34998	2.2	1.9	6.301	519.5	2.7	0.287296
19	6.44	556	49.5	0.0858564	4	0.522098	0.9	0.8	6.44	554.4	3.6	0.0827546
20	6.467	563	50.1	0.0827656	1	0.200595	0.3	0.3	6.467	561.8	1.2	0.0918849
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.486	568	50.5	0.495906	3	3.03843	5	4.4	6.486	569	3.4	0.506391
22	6.521	577	51.3	0.204397	2	0.701203	1.2	1	6.521	577.5	1.9	0.210765
23	6.554	585	52	0.399408	2	1.36461	2.3	2	6.554	584.6	1.8	0.427471
24	6.627	594	52.8	0.310075	3	1.72281	2.9	2.5	6.627	593.3	3.1	0.318607
25	6.684	601	53.5	0.455443	2	1.50382	2.5	2.2	6.684	601.5	1.8	0.470308
26	7.11	669	59.5	0.572644	3	2.83349	4.7	4.1	7.11	669.4	2.7	0.583915
27	7.235	692	61.6	0.105723	3	0.559591	0.9	0.8	7.235	691.8	3.1	0.102519
28	7.371	717	63.8	0.491679	4	3.5278	5.9	5.1	7.371	716	4.2	0.470476
29	7.561	754	67.1	0.478491	4	3.4059	5.7	4.9	7.561	753.5	4.2	0.460511
30	7.669	775	69	0.139104	3	0.69989	1.2	1	7.669	775	2.9	0.136532
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.859	812	72.2	0.351746	7	4.38877	7.3	6.3	7.859	811.4	7	0.355129
32	8.481	942	83.8	0.127522	16	3.3998	5.6	4.9	8.481	946.1	16	0.119676
33	8.633	974	86.7	0.308925	10	5.22895	8.7	7.5	8.633	974.1	10.5	0.28226
34	8.865	1022	90.9	0.312302	8	4.48769	7.5	6.5	8.865	1019.3	8.4	0.302619
35	9.112	1073	95.5	0.219002	10	3.18992	5.3	4.6	9.112	1069.2	9.6	0.186941

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	18	1.6	0.299249	13	6.60597	17.1	14.3	3.5	23.8	12.9	0.288399
2	4.55	180	16	0.430973	5	3.07694	8	6.7	4.55	180	4.6	0.374772
3	5.2	258	23	0.592916	4	4.10021	10.6	8.9	5.2	257.8	4	0.574587
4	5.85	395	35.1	0.668336	3	4.17256	10.8	9	5.85	394.7	3.5	0.67522
5	6.55	582	51.8	0.555663	4	4.24356	11	9.2	6.55	581.5	4.4	0.54134
6	6.85	618	55	0.275987	5	2.35167	6.1	5.1	6.85	617.6	5.3	0.248542
7	7.35	711	63.3	0.554485	5	4.92332	12.7	10.6	7.35	709.8	5	0.556122
8	8.15	866	77	0.10072	11	1.86951	4.8	4	8.15	865.8	11	0.0957145
9	8.45	935	83.2	0.0985609	8	1.34027	3.5	2.9	8.45	935.5	8	0.0945462
10	8.65	977	86.9	0.146481	5	1.28608	3.3	2.8	8.65	976.8	5.3	0.137745
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1112	98.9	0.590543	5	4.71634	12.2	10.2	9.3	1111.3	4.5	0.586096

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP CO MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 136

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 18.600 4.550 8.100 5.200 10.400 5.850 10.200 6.550 9.800 6.850 5.900 7.350 12.100 8.150 4.900 8.450 4.200 8.650 3.400 9.300 12.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
4.682 1.400 4.909 1.000 5.073 0.200 5.182 2.200 5.287 2.000 5.350 0.300 5.379 1.600 5.504 2.400 5.537 2.700 5.654 2.200 5.704 0.600 6.008 3.600 6.103 1.900 6.158 3.200 6.295 1.800 6.454 1.700 6.509 5.700 6.548 1.000 6.595 1.900 6.665 3.200 6.707 1.800 7.248 2.100 7.378 4.200 7.506 3.800 7.686 0.900 7.768 4.600 8.497 7.000 8.632 19.900 8.863 8.800 9.097 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 30 Return to Lane Navigator
3.500 5.600 4.550 3.800 4.880 0.400 4.973 0.200 5.066 0.600 5.200 2.900 5.281 3.300 5.382 1.500 5.507 2.100 5.539 2.100 5.600 1.600 5.656 1.700 5.709 0.600 5.850 4.000 6.015 3.300 6.113 1.700 6.175 2.600 6.323 1.400 6.492 1.400 6.550 5.500 6.615 1.000 6.661 1.700 6.726 2.500 6.772 1.700 6.850 1.300 7.165 2.900 7.350 5.400 7.424 3.200 7.603 3.900 7.745 0.600 7.882 3.900 8.150 3.500 8.450 0.900 8.532 2.500 8.650 6.100 8.862 5.000 9.103 3.900 9.300 3.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 38 Return to Lane Navigator
3.500 21.700 4.550 7.400 5.200 9.600 5.850 9.200 6.550 9.300 6.850 6.000 7.350 10.900 8.150 4.300 8.450 3.300 8.650 2.700 9.300 15.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
4.656 1.100 4.859 1.100 4.924 0.800 4.989 0.500 5.102 2.800 5.246 2.000 5.297 0.600 5.353 1.300 5.520 1.900 5.548 2.900 5.613 1.700 5.683 3.000 5.799 0.700 5.881 0.700 6.039 3.300 6.108 2.700 6.166 2.800 6.301 2.200 6.440 0.900 6.467 0.300 6.486 5.000 6.521 1.200 6.554 2.300 6.627 2.900 6.684 2.500 7.110 4.700 7.235 0.900 7.371 5.900 7.561 5.700 7.669 1.200 7.859 7.300 8.481 5.600 8.633 8.700 8.865 7.500 9.112 5.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 7 LANE TYPE: Specimen ACTUAL GEL LANE: 9 PeakCount: 35 Return to Lane Navigator
3.500 17.100 4.550 8.000 5.200 10.600 5.850 10.800 6.550 11.000 6.850 6.100 7.350 12.700 8.150 4.800 8.450 3.500 8.650 3.300 9.300 12.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 11 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Sockeye Salmon ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Sockeye Salmon ---- Go to Display Gel Lane Navigator.