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1: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):43-5. Epub 2004 Oct 16.Click here to read Links

Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii.

Kitao T, Kuroishi C, Tahirov TH.

Highthroughput Factory, RIKEN Harima Institute, 1-1-1 Kouto, Hyogo 679-5148, Japan.

The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P2(1), with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 A, beta = 116.07 degrees, and diffract to 2.2 A. The PhNikR-Ni-1 crystals belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 99.89, c = 97.98 A, and diffract to 3.0 A and the PhNikR-Ni-2 crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 109.95, c = 79.0 A, and diffract to 2.1 A. The crystals obtained were suitable for detailed structural studies.

PMID: 16508086 [PubMed - indexed for MEDLINE]

PMCID: PMC1952384

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