BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= 'E13F05_L05_11.ab1' (552 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 3 Sequences     : less than 3 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 614 135 |=============================================
   6310 479  73 |========================
   3980 406 119 |=======================================
   2510 287  95 |===============================
   1580 192  67 |======================
   1000 125  21 |=======
    631 104  21 |=======
    398  83  20 |======
    251  63  19 |======
    158  44   3 |=
    100  41   5 |=
   63.1  36  15 |=====
   39.8  21   4 |=
   25.1  17   7 |==
   15.8  10   1 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 9  <<<<<<<<<<<<<<<<<
   10.0   9   1 |:
   6.31   8   2 |:
   3.98   6   2 |:
   2.51   4   0 |
   1.58   4   0 |
   1.00   4   0 |
   0.63   4   0 |
   0.40   4   0 |
   0.25   4   1 |:
   0.16   3   1 |:
   0.10   2   0 |
  0.063   2   0 |
  0.040   2   0 |
  0.025   2   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|9994098|gb|AAG12126.1|AC079730_22(AC079730) hypoth... +1   115  0.00023   1
gi|8778403|gb|AAF79411.1|AC068197_21(AC068197) F16A14... +1   100  0.023     1
gi|7487150|pir||T02697hypothetical protein T18E12.4 -... +1    94  0.11      1
gi|3087719|emb|CAA76642.1|(Y17129) S31 protein [Angui... +1    76  0.15      1
gi|5852697|gb|AAD54172.1|AF134972_1(AF134972) MHC cla... +3    45  0.97      2
gi|5834873ref|NP_006942.1|ND4L_10020 NADH dehydrogena... +1    64  0.97      1
gi|11355301|pir||F82093hypothetical protein VC2317 [i... -3    63  0.99      1
gi|6630552|gb|AAF19571.1|AC011708_14(AC011708) putati... +1    81  0.990     1
gi|12597802|gb|AAG60114.1|AC073178_25(AC073178) unkno... +1    78  0.99991   1

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|9994098|gb|AAG12126.1|AC079730_22  (AC079730) hypothetical protein;
            33506-35224 [Arabidopsis thaliana]
            >gi|12321358|gb|AAG50754.1|AC079733_22 (AC079733) integral membrane
            protein, putative [Arabidopsis thaliana]
            Length = 212

Frame  1 hits (HSPs):                                             ________
                        __________________________________________________
Database sequence:     |           |           |           |          |   | 212
                       0          50         100         150        200

  Plus Strand HSPs:

 Score = 115 (40.5 bits), Expect = 0.00023, P = 0.00023
 Identities = 22/30 (73%), Positives = 27/30 (90%), Frame = +1

Query:     1 FSIFSLGICILVSGLQLWYLKRFFRKKKLI 90
             +SI SLGICI+VSGLQ+ YLK++F KKKLI
Sbjct:   183 YSIMSLGICIVVSGLQILYLKQYFEKKKLI 212


to_Entrezto_Relatedto_Related >gi|8778403|gb|AAF79411.1|AC068197_21  (AC068197) F16A14.23 [Arabidopsis
            thaliana]
            Length = 221

Frame  1 hits (HSPs):                                              _______
                        __________________________________________________
Database sequence:     |           |          |          |           |    | 221
                       0          50        100        150         200

  Plus Strand HSPs:

 Score = 100 (35.2 bits), Expect = 0.023, P = 0.023
 Identities = 18/29 (62%), Positives = 23/29 (79%), Frame = +1

Query:     4 SIFSLGICILVSGLQLWYLKRFFRKKKLI 90
             S  SL +C+ V+GLQ W+LK FF+KKKLI
Sbjct:   193 SFVSLAVCLSVAGLQFWHLKTFFQKKKLI 221


to_Entrezto_Relatedto_Related >gi|7487150|pir||T02697  hypothetical protein T18E12.4 - Arabidopsis thaliana
            >gi|4335768|gb|AAD17445.1| (AC006284) putative Golgi-associated
            membrane trafficking protein [Arabidopsis thaliana]
            Length = 213

Frame  1 hits (HSPs):                                              _______
                        __________________________________________________
Database sequence:     |           |           |          |           |   | 213
                       0          50         100        150         200

  Plus Strand HSPs:

 Score = 94 (33.1 bits), Expect = 0.12, P = 0.11
 Identities = 18/29 (62%), Positives = 22/29 (75%), Frame = +1

Query:     4 SIFSLGICILVSGLQLWYLKRFFRKKKLI 90
             S  SL +C+ V+GLQ W+LK FF KKKLI
Sbjct:   185 SFGSLVVCLSVAGLQFWHLKTFFEKKKLI 213


to_Entrezto_Relatedto_Related >gi|3087719|emb|CAA76642.1|  (Y17129) S31 protein [Anguilla anguilla]
            Length = 48

Frame  1 hits (HSPs):                    ________________________________ 
                        __________________________________________________
Database sequence:     |                   |                    |         | 48
                       0                  20                   40

  Plus Strand HSPs:

 Score = 76 (26.8 bits), Expect = 0.16, P = 0.15
 Identities = 19/30 (63%), Positives = 23/30 (76%), Frame = +1

Query:     1 FSIFSLGICILVSGLQLW---YLKRFFRKKKLI 90
             FSIFS+  C L+ GL  W   YL+RFF+ KKLI
Sbjct:    18 FSIFSM--CCLI-GLATWQVFYLRRFFKAKKLI 47


to_Entrezto_Relatedto_Related >gi|5852697|gb|AAD54172.1|AF134972_1  (AF134972) MHC class II antigen [Psettodes
            sp.]
            Length = 63

Frame  3 hits (HSPs):                  ______________________             
Frame  1 hits (HSPs):     _______________                                 
                        __________________________________________________
Database sequence:     |               |              |               |   | 63
                       0              20             40              60

  Plus Strand HSPs:

 Score = 45 (15.8 bits), Expect = 3.4, Sum P(2) = 0.97
 Identities = 13/33 (39%), Positives = 16/33 (48%), Frame = +3

Query:   234 QELESLVRSWR*WNDGDWF*LLSIYYIYEFLRH 332
             QE+ S V S     DGDW+     Y I+  L H
Sbjct:    20 QEVTSDVTSTEELADGDWY-----YQIHSHLEH 47

 Score = 40 (14.1 bits), Expect = 3.4, Sum P(2) = 0.97
 Identities = 9/19 (47%), Positives = 10/19 (52%), Frame = +1

Query:   106 YETYPHQIVFSSFRDDSNI 162
             YE YP QI  S  RD   +
Sbjct:     4 YEFYPKQIRVSWTRDGQEV 22


to_Entrezto_Related >gi|5834873  ref|NP_006942.1|ND4L_10020 NADH dehydrogenase subunit 4L [Ascaris
            suum] >gi|1171867|sp|P24874|NULM_ASCSU NADH-UBIQUINONE
            OXIDOREDUCTASE CHAIN 4L >gi|102586|pir||S26015 NADH dehydrogenase
            (ubiquinone) (EC 1.6.5.3) chain 4L - pig roundworm mitochondrion
            >gi|559489|emb|CAA38164.1| (X54253) ND4L protein [Ascaris suum]
            Length = 77

Frame  1 hits (HSPs):       ___________________________________           
                        __________________________________________________
Database sequence:     |            |            |            |           | 77
                       0           20           40           60

  Plus Strand HSPs:

 Score = 64 (22.5 bits), Expect = 3.6, P = 0.97
 Identities = 18/54 (33%), Positives = 29/54 (53%), Frame = +1

Query:    55 YLKRFFRKKKLI*ILLSYETYPHQIVFSSFRDDSNIV*FFFLWILSCVIGVCGL 216
             +L  FF+ ++L+ IL+S E +    +F  F  D N + FF+    S V  V G+
Sbjct:     8 FLSLFFKWQRLMFILISLE-FIVMSLFILFSGDLNEMMFFYFMCFSVVSSVLGM 60


to_Entrezto_Relatedto_Related >gi|11355301|pir||F82093  hypothetical protein VC2317 [imported] - Vibrio
            cholerae  (group O1 strain N16961) >gi|9656882|gb|AAF95461.1|
            (AE004301) hypothetical protein [Vibrio cholerae]
            Length = 54

Frame -3 hits (HSPs):                            ______________________   
                        __________________________________________________
Database sequence:     |                 |                  |             | 54
                       0                20                 40

  Minus Strand HSPs:

 Score = 63 (22.2 bits), Expect = 4.6, P = 0.99
 Identities = 10/25 (40%), Positives = 19/25 (76%), Frame = -3

Query:   523 CTNIRLIHSSIHILFHLINSISVKH 449
             C N+ L+ +S+H LF+L+N + ++H
Sbjct:    28 CNNLHLLFTSVHTLFNLLN-LKLQH 51


to_Entrezto_Relatedto_Related >gi|6630552|gb|AAF19571.1|AC011708_14  (AC011708) putative membrane protein
            [Arabidopsis thaliana]
            Length = 217

Frame  1 hits (HSPs):                                              _______
                        __________________________________________________
Database sequence:     |           |          |           |          |    | 217
                       0          50        100         150        200

  Plus Strand HSPs:

 Score = 81 (28.5 bits), Expect = 4.6, P = 0.99
 Identities = 17/28 (60%), Positives = 22/28 (78%), Frame = +1

Query:     7 IFSLGICILVSGLQLWYLKRFFRKKKLI 90
             + SLG+ I+VS  Q+ YLKR+F KKKLI
Sbjct:   190 LMSLGVAIVVSISQVLYLKRYFLKKKLI 217


to_Entrezto_Relatedto_Related >gi|12597802|gb|AAG60114.1|AC073178_25  (AC073178) unknown protein [Arabidopsis
            thaliana]
            Length = 204

Frame  1 hits (HSPs):                                             ________
                        __________________________________________________
Database sequence:     |            |           |           |           | | 204
                       0           50         100         150         200

  Plus Strand HSPs:

 Score = 78 (27.5 bits), Expect = 9.3, P = 1.0
 Identities = 15/29 (51%), Positives = 21/29 (72%), Frame = +1

Query:     4 SIFSLGICILVSGLQLWYLKRFFRKKKLI 90
             S+ S  +CI V+G+Q  +LK FF KKK+I
Sbjct:   176 SVLSFFVCIGVAGMQFLHLKTFFEKKKVI 204


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.98

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.355   0.159   0.620  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.354   0.158   0.570  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.361   0.165   0.617  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.347   0.153   0.501  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.345   0.150   0.499  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.361   0.157   0.561  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      183       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   +2      0      183       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   +1      0      184       184       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -1      0      184       184       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -2      0      183       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37
   -3      0      183       183       10.  76 3  12 22  0.12    34
                                                    31  0.12    37


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  9
  No. of states in DFA:  594 (59 KB)
  Total size of DFA:  219 KB (256 KB)
  Time to generate neighborhood:  0.02u 0.00s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  182.88u 1.06s 183.94t  Elapsed: 00:00:31
  Total cpu time:  182.90u 1.09s 183.99t  Elapsed: 00:00:31
  Start:  Wed Jan 23 18:25:21 2002   End:  Wed Jan 23 18:25:52 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000