Project Number: 5306-21000-016-01
Project Type: Reimbursable

Start Date: Jul 04, 2004
End Date: Aug 31, 2006

Objective:
Develop a 10,000 individual EMS population of rice (cv. Nipponbare). Provide tissue samples to Seattle and arrange for public distribution of seeds. Characterize some tilled genes in Nipponbare by simple genetic and phenotypic analyzes.

Approach:
Seeds of the cv. Nipponbare will be subjected to treatment with various chemical mutagens known to induced point mutations and small deletions in DNA. Seeds will be planted and allowed to reproduce. Single panicles from these plants will be harvested and single seed from each of these panicles will be planted. Leaf tissue will be harvested and freeze dried (i.e. preserved) from this generation of plants, which will also be allowed to produce new seeds. Freeze dried tissue from plants producing sufficient amounts of seed will be sent to our cooperators, who will extract DNA samples and analyze them for mutations in a pre-determined set of rice genes. Seeds from plants whose tissue samples yielded detectable mutations in genes of interest will be planted and the resulting plants will be used for simple genetic and phenotypic characterization. Documents Reimbursable with University of Washington/NRI. Log 24824.