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ix Contents Preface ............................................................................................................... Contributors ....................................................................................................... 1 Treatment of Tissue Sections for In Situ Hybridization Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson ............................................................ 2 Probe Design Peter Roche ............................................................................................. 3 cRNA Probes: Comparison of Isotopic and Nonisotopic Detection Methods Teresa Bisucci, Tim D. Hewitson, and Ian A. Darby .............................. 4 Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization Ernst J. M. Speel, Anton H. N. Hopman, and Paul Komminoth .......................................................................... 5 Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Non-Radioactively Labeled Probes Anne Chotteau-Leli--- vre, Pascal Doll--- , and Fran---- oise Gofflot ......................................................................... 6 Non-Radioactive In Situ Hybridization on Frozen Sections and Whole Mounts Julia Asp, Alexandra Abramsson, and Christer Betsholtz....................... 7 In Situ Hybridization of Whole Mount Embryos Murray Hargrave, Josephine Bowles, and Peter Koopman .................... 8 In Situ Detection of Epstein-Barr Virus and Phenotype Determination of EBV-Infected Cells Gerald Niedobitek and Hermann Herbst ............................................... 9 PNA- Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent Detection of Cellular and Viral Proteins Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda .................... 10 Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum Andrzej Loesch and Rainer Glass ........................................................... 11 Free-Floating Sections of Brain In Situ Neil Owens ............................................................................................. 12 An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells Changsun Choi, Laurie G. Hudson, Pierre Savagner, and Donna F. Kusewitt ....................................................................... 13 Identification of Transplanted Human Cells in Animal Tissues Daniel Benten, Kang Cheng, and Sanjeev Gupta ................................... 14 In Situ Hybridisation to Plant Tissues and Chromosomes Andreas Houben, Sharon J. Orford, and Jeremy N. Timmis ........................................................................ 15 Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby ................................................................................ 16 Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby .............................. 17 The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Co-Localize mRNA and Protein in Tissue Sections Malcolm D. Smith, Michael Ahern, and Mark Coleman ........................ 18 Semi-Quantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations Paul D. Storer and Tracey DeLucia ........................................................ 19 Quantitative In Situ Hybridization of Tissue Microarrays Adrian M. Jubb, Thinh Q. Pham, Gretchen D. Frantz, Frank V. Peale, and Kenneth J. Hillan ................................................
Library of Congress Subject Headings for this publication:
In situ hybridization -- Laboratory manuals.
In Situ Hybridization -- Laboratory Manuals.