2006 Annual Report 4d.Progress report. This report serves to document research conducted under a Specific Cooperative Agreement (Project No. 58-5306-4-479) between ARS and the University of California, Davis. Roughly 100 grape accessions of presumed Iberian origin were analyzed using a standard set of 8 SSR markers. The resulting profiles are being compared to profiles provided by collaborators at national grape germplasm collections in Dois Portos, Portugal and El Encin, Spain. Since these collections can be considered to retain the "type" material for their respective countries, these data will be used to verify the identity of NCGR accessions. Several other groups of NCGR grape accessions were analyzed with the same 8 SSR markers: 30 central and South American accessions, 35 accessions from Afghanistan, accessions or possible parents from the Cornell University grape breeding program and 30 accessions associated with the general grape/wine name "Malvasia". New DNA extractions and fingerprinting was initiated on Italian grapes and the Greek grapes not previously tested. These data will then be used to gain inferences on the relatedness and origins of these sub collections. In total we now have data on SSRs, DNA extracted, or leaves collected for DNA extraction for 2302 vines of the 6000 in the NCGR Vitis collection. Before the end of the fiscal year we will have collected leaves for the 474 remaining vines of V. vinifera. Additional details of the research can be found in the report for the parent CRIS 5306-21000-0150-OOD, Management of Fruit and Nut Crop Genetic Resources.