Bibliographic Citation
Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
---|---|
Title | Stereospecific hydroxylation of indan by Escherichia coli containing the cloned toluene dioxygenase genes from Pseudomonas putida F1 |
Creator/Author | Brand, J.M. ; Cruden, D.L. ; Zylstra, G.J. ; Gibson, D.T. (Univ. of Iowa, Iowa City (United States)) |
Publication Date | 1992 Oct 01 |
OSTI Identifier | OSTI ID: 7224317 |
Other Number(s) | ISSN0099-2240; CODEN: AEMID |
Resource Type | Journal Article |
Resource Relation | Applied and Environmental Microbiology ; Vol/Issue: 58:10 |
Subject | 550400 -- Genetics ;560300 -- Chemicals Metabolism & Toxicology; ESCHERICHIA COLI-- GENETIC ENGINEERING;INDAN-- HYDROXYLATION;OXYGENASES-- DNA-CLONING;TOLUENE-- BIODEGRADATION; PSEUDOMONAS;RECOMBINANT DNA;STEREOCHEMISTRY |
Related Subject | ALKYLATED AROMATICS;AROMATICS;BACTERIA;BIOTECHNOLOGY;CHEMICAL REACTIONS;CLONING;DECOMPOSITION;DNA;DNA HYBRIDIZATION;ENZYMES;HYBRIDIZATION;HYDROCARBONS;MICROORGANISMS;NUCLEIC ACIDS;ORGANIC COMPOUNDS;OXIDOREDUCTASES;PROTEINS |
Description/Abstract | Escheria coli JM109(pDTG601), containing the todC1C2BA genes encoding toluene dioxygenase from Pseudomonas putida F1, oxides indan to (-)-(1R)-indanol (83{percent} R) and trans-1,3-indandiol.^Under similar conditions, P.putida F39/D oxidizes indan to (-)-(1R)-indanol (96{percent}R), 1-indanone, and trans-1,3-indandiol.^The differences in the enantiomeric composition of the 1-indanols formed by the two organisms are due to the presence of a 1-indanol dehydrogenase in P.putida F39/D that preferentially oxidizes (+)-(1S)-indanol. |
Country of Publication | United States |
Language | English |
Format | Pages: 3407-3409 |
System Entry Date | 2001 May 13 |
Top |