BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= 'E04F12_F12_12.ab1' (602 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 3 Sequences     : less than 3 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 915 168 |========================================================
   6310 747 170 |========================================================
   3980 577 140 |==============================================
   2510 437 149 |=================================================
   1580 288 101 |=================================
   1000 187  72 |========================
    631 115  43 |==============
    398  72  16 |=====
    251  56  18 |======
    158  38   8 |==
    100  30   9 |===
   63.1  21   3 |=
   39.8  18   2 |:
   25.1  16   3 |=
   15.8  13   1 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 12  <<<<<<<<<<<<<<<<<
   10.0  12   1 |:
   6.31  11   0 |
   3.98  11   0 |
   2.51  11   0 |
   1.58  11   0 |
   1.00  11   0 |
   0.63  11   0 |
   0.40  11   0 |
   0.25  11   0 |
   0.16  11   0 |
   0.10  11   0 |
  0.063  11   0 |
  0.040  11   0 |
  0.025  11   0 |
  0.016  11   0 |
  0.010  11   0 |
 0.0063  11   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|12407755|gb|AAG53638.1|AF291714_1(AF291714) initia... +1   305  3.6e-26   1
gi|6685535|sp|P56820|IF37_ARATHPUTATIVE EUKARYOTIC TR... +1   305  1.9e-25   1
gi|10176887|dbj|BAB10117.1|(AB011475) eukaryotic tran... +1   302  4.0e-25   1
gi|4503523ref|NP_003744.1| eukaryotic translation ini... +1   138  2.1e-06   1
gi|9055214ref|NP_061219.1| eIF3 p66 [Mus musculus] >g... +1   134  8.8e-06   1
gi|7299570|gb|AAF54756.1|(AE003694) CG4810 gene produ... +1   126  0.00011   1
gi|7141239|gb|AAF37264.1|AF220363_1(AF220363) eukaryo... +1   126  0.00011   1
gi|7301022|gb|AAF56158.1|(AE003744) eIF-3p66 gene pro... +1   126  0.00011   1
gi|7492557|pir||T38999probable elongation initation f... +1   122  0.00035   1
gi|6685653|sp|O94236|MOE1_SCHPOMICROTUBULE DESTABILIZ... +1   122  0.00035   1
gi|267549|sp|P30642|IF37_CAEELPUTATIVE EUKARYOTIC TRA... +1   112  0.0060    1
gi|8843986|gb|AAF80206.1|AF282853_5(AF282853) CagP [H... +3    71  0.9999    1

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|12407755|gb|AAG53638.1|AF291714_1  (AF291714) initiation factor 3d
            [Arabidopsis thaliana]
            Length = 418

Frame  1 hits (HSPs):                                           __________
                        __________________________________________________
Database sequence:     |                 |                 |              | 418
                       0               150               300

  Plus Strand HSPs:

 Score = 305 (107.4 bits), Expect = 3.6e-26, P = 3.6e-26
 Identities = 59/76 (77%), Positives = 64/76 (84%), Frame = +1

Query:    28 SIVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPEDEQVQPPSEGAEGG 207
             SIVDLCMKL+EGKYVLVKDPSKPQVRIYEVP DAFENDYVEEPLPEDEQVQP  E  EG 
Sbjct:   339 SIVDLCMKLSEGKYVLVKDPSKPQVRIYEVPPDAFENDYVEEPLPEDEQVQPTEENTEGA 398

Query:   208 EATTT-TNDVEDKQID 252
             EA+   T + E+K+ D
Sbjct:   399 EASVAATKETEEKKAD 414


to_Entrezto_Relatedto_Related >gi|6685535|sp|P56820|IF37_ARATH  PUTATIVE EUKARYOTIC TRANSLATION INITIATION
            FACTOR 3 SUBUNIT 7 (EIF-3 ZETA) >gi|7486971|pir||T10640
            hypothetical protein T13K14.140 - Arabidopsis thaliana
            >gi|5262788|emb|CAB45893.1| (AL080282) translation initiation
            factor eIF3-like protein [Arabidopsis thaliana]
            >gi|7268895|emb|CAB79098.1| (AL161554) translation initiation
            factor eIF3-like protein [Arabidopsis thaliana]
            Length = 591

Frame  1 hits (HSPs):                                              _______
                        __________________________________________________
Database sequence:     |            |            |           |            | 591
                       0          150          300         450

  Plus Strand HSPs:

 Score = 305 (107.4 bits), Expect = 1.9e-25, P = 1.9e-25
 Identities = 59/76 (77%), Positives = 64/76 (84%), Frame = +1

Query:    28 SIVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPEDEQVQPPSEGAEGG 207
             SIVDLCMKL+EGKYVLVKDPSKPQVRIYEVP DAFENDYVEEPLPEDEQVQP  E  EG 
Sbjct:   512 SIVDLCMKLSEGKYVLVKDPSKPQVRIYEVPPDAFENDYVEEPLPEDEQVQPTEENTEGA 571

Query:   208 EATTT-TNDVEDKQID 252
             EA+   T + E+K+ D
Sbjct:   572 EASVAATKETEEKKAD 587


to_Entrezto_Relatedto_Related >gi|10176887|dbj|BAB10117.1|  (AB011475) eukaryotic translation initiation
            factor 3 subunit 7 [Arabidopsis thaliana]
            Length = 588

Frame  1 hits (HSPs):                                              _______
                        __________________________________________________
Database sequence:     |            |            |            |           | 588
                       0          150          300          450

  Plus Strand HSPs:

 Score = 302 (106.3 bits), Expect = 4.0e-25, P = 4.0e-25
 Identities = 61/82 (74%), Positives = 69/82 (84%), Frame = +1

Query:    28 SIVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPEDEQVQPPSEGAEGG 207
             SIVDLCMKL+EGKYVLVKDPSKPQVRIYEVPADAF+NDYVEEPLPEDEQVQPP E  + G
Sbjct:   507 SIVDLCMKLSEGKYVLVKDPSKPQVRIYEVPADAFDNDYVEEPLPEDEQVQPPEENTDAG 566

Query:   208 EAT---TTTN-DVEDKQIDGQA 261
               T   ++TN  VEDK+ + +A
Sbjct:   567 AETNGVSSTNVAVEDKKSEVEA 588


to_Entrezto_Related >gi|4503523  ref|NP_003744.1| eukaryotic translation initiation factor 3,
            subunit 7 (zeta, 66/67kD) [Homo sapiens] >gi|11418048
            ref|XP_009954.1| eukaryotic translation initiation factor 3,
            subunit 7 (zeta, 66/67kD) [Homo sapiens]
            >gi|6685526|sp|O15371|IF37_HUMAN EUKARYOTIC TRANSLATION INITIATION
            FACTOR 3 SUBUNIT 7 (EIF-3 ZETA) (EIF3 P66)
            >gi|2351378|gb|AAD03466.1| (U54558) translation initiation factor
            eIF3 p66 subunit [Homo sapiens] >gi|4200328|emb|CAA18440.1|
            (AL022313) dJ1119A7.2 (eukaryotic translation initiation factor 3,
            subunit 7 (zeta, 66/67kD) (EIF3-P66)) [Homo sapiens]
            >gi|12653123|gb|AAH00328.1|AAH00328 (BC000328) eukaryotic
            translation initiation factor 3, subunit 7 (zeta, 66/67kD) [Homo
            sapiens] >gi|12653399|gb|AAH00469.1|AAH00469 (BC000469) eukaryotic
            translation initiation factor 3, subunit 7 (zeta, 66/67kD) [Homo
            sapiens]
            Length = 548

Frame  1 hits (HSPs):                                               ______
                        __________________________________________________
Database sequence:     |             |             |            |         | 548
                       0           150           300          450

  Plus Strand HSPs:

 Score = 138 (48.6 bits), Expect = 2.1e-06, P = 2.1e-06
 Identities = 23/54 (42%), Positives = 37/54 (68%), Frame = +1

Query:    31 IVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPEDEQVQPPSE 192
             ++D+CMKL EGKY+++KDP+K  +R+Y +P   F +D  EE   E+E+ +   E
Sbjct:   494 VIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDEEEEEEEEEEEEEEE 547


to_Entrezto_Related >gi|9055214  ref|NP_061219.1| eIF3 p66 [Mus musculus]
            >gi|6685529|sp|O70194|IF37_MOUSE EUKARYOTIC TRANSLATION INITIATION
            FACTOR 3 SUBUNIT 7 (EIF-3 ZETA) (EIF3 P66)
            >gi|2992164|dbj|BAA25327.1| (AB012580) eIF3 p66 [Mus musculus]
            Length = 547

Frame  1 hits (HSPs):                                               ______
                        __________________________________________________
Database sequence:     |             |             |             |        | 547
                       0           150           300           450

  Plus Strand HSPs:

 Score = 134 (47.2 bits), Expect = 8.8e-06, P = 8.8e-06
 Identities = 22/54 (40%), Positives = 37/54 (68%), Frame = +1

Query:    31 IVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPEDEQVQPPSE 192
             ++D+CMKL EGKY+++KDP+K  +R+Y +P   F ++  EE   E+E+ +   E
Sbjct:   493 VIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSEEDEEDEEEEEEEEEEEE 546


to_Entrezto_Relatedto_Related >gi|7299570|gb|AAF54756.1|  (AE003694) CG4810 gene product [Drosophila
            melanogaster]
            Length = 551

Frame  1 hits (HSPs):                                                _____
                        __________________________________________________
Database sequence:     |             |             |            |         | 551
                       0           150           300          450

  Plus Strand HSPs:

 Score = 126 (44.4 bits), Expect = 0.00011, P = 0.00011
 Identities = 21/46 (45%), Positives = 35/46 (76%), Frame = +1

Query:    31 IVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPED 168
             ++DL M+  +GKY+++KDP+KP +R+Y+VP +AF++D  EE    D
Sbjct:   498 LIDLVMRQPDGKYLIMKDPNKPMIRLYDVPENAFDSDGDEEEESSD 543


to_Entrezto_Relatedto_Related >gi|7141239|gb|AAF37264.1|AF220363_1  (AF220363) eukaryotic translation
            initiation factor 3 p66 subunit [Drosophila melanogaster]
            Length = 560

Frame  1 hits (HSPs):                                               ______
                        __________________________________________________
Database sequence:     |             |            |             |         | 560
                       0           150          300           450

  Plus Strand HSPs:

 Score = 126 (44.4 bits), Expect = 0.00011, P = 0.00011
 Identities = 21/50 (42%), Positives = 37/50 (74%), Frame = +1

Query:    31 IVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPEDEQVQ 180
             I+DL MK  +GKY+++KDP+KP +R+Y++P + F++D  ++   +DE  Q
Sbjct:   501 IIDLVMKQKDGKYLIMKDPNKPIIRLYDIPDNTFDSDDSDDGEGDDEGFQ 550


to_Entrezto_Relatedto_Related >gi|7301022|gb|AAF56158.1|  (AE003744) eIF-3p66 gene product [Drosophila
            melanogaster]
            Length = 560

Frame  1 hits (HSPs):                                               ______
                        __________________________________________________
Database sequence:     |             |            |             |         | 560
                       0           150          300           450

  Plus Strand HSPs:

 Score = 126 (44.4 bits), Expect = 0.00011, P = 0.00011
 Identities = 21/50 (42%), Positives = 37/50 (74%), Frame = +1

Query:    31 IVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYVEEPLPEDEQVQ 180
             I+DL MK  +GKY+++KDP+KP +R+Y++P + F++D  ++   +DE  Q
Sbjct:   501 IIDLVMKQKDGKYLIMKDPNKPIIRLYDIPDNTFDSDDSDDGEGDDEGFQ 550


to_Entrezto_Relatedto_Related >gi|7492557|pir||T38999  probable elongation initation factor subunit - fission
            yeast (Schizosaccharomyces pombe) >gi|4056551|emb|CAA22586.1|
            (AL034583) elongation initation factor subunit; negative regulator
            moe1.; microtubule destabilising protein [Schizosaccharomyces
            pombe]
            Length = 567

Frame  1 hits (HSPs):                                                ____ 
                        __________________________________________________
Database sequence:     |             |            |            |          | 567
                       0           150          300          450

  Plus Strand HSPs:

 Score = 122 (42.9 bits), Expect = 0.00035, P = 0.00035
 Identities = 20/36 (55%), Positives = 30/36 (83%), Frame = +1

Query:    28 SIVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFE 135
             +I D+C+K+ +GKYVLVKDP++P +R+Y VP + FE
Sbjct:   519 TIADVCLKMPDGKYVLVKDPNRPILRLYSVPPNTFE 554


to_Entrezto_Relatedto_Related >gi|6685653|sp|O94236|MOE1_SCHPO  MICROTUBULE DESTABILIZING PROTEIN MOE1
            >gi|11281983|pir||T43555 Ras pathway interacting protein Moe1 -
            fission yeast  (Schizosaccharomyces pombe)
            >gi|4176721|gb|AAD08893.1| (AF038568) negative regulator Moe1
            [Schizosaccharomyces pombe]
            Length = 567

Frame  1 hits (HSPs):                                                ____ 
                        __________________________________________________
Database sequence:     |             |            |            |          | 567
                       0           150          300          450

  Plus Strand HSPs:

 Score = 122 (42.9 bits), Expect = 0.00035, P = 0.00035
 Identities = 20/36 (55%), Positives = 30/36 (83%), Frame = +1

Query:    28 SIVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFE 135
             +I D+C+K+ +GKYVLVKDP++P +R+Y VP + FE
Sbjct:   519 TIADVCLKMPDGKYVLVKDPNRPILRLYSVPPNTFE 554


to_Entrezto_Relatedto_Related >gi|267549|sp|P30642|IF37_CAEEL  PUTATIVE EUKARYOTIC TRANSLATION INITIATION
            FACTOR 3 SUBUNIT 7 (EIF-3 ZETA) >gi|102510|pir||S24459 hypothetical
            protein R08D7.3 - Caenorhabditis elegans
            >gi|3878936|emb|CAA78049.1| (Z12017) predicted using
            Genefinder~cDNA EST yk105g8.3 comes from this gene~cDNA EST
            yk105g8.5 comes from this gene~cDNA EST yk152a2.3 comes from this
            gene~cDNA EST yk152a2.5 comes from this gene~cDNA EST yk490b3.3
            comes from this gene~cDNA EST yk490b3>
            Length = 570

Frame  1 hits (HSPs):                                                _____
Annotated Domains:      _________________________________________________ 
                        __________________________________________________
Database sequence:     |             |            |            |          | 570
                       0           150          300          450
__________________

Annotated Domains:
   PRODOM               PD025385: O15371(1) O70194(1) YNE3(1)    3..555
__________________


  Plus Strand HSPs:

 Score = 112 (39.4 bits), Expect = 0.0060, P = 0.0060
 Identities = 20/50 (40%), Positives = 32/50 (64%), Frame = +1

Query:    31 IVDLCMKLNEGKYVLVKDPSKPQVRIYEVPADAFENDYV--EEPLPEDEQ 174
             ++D CMK   GKY+L+KDP  P +R+Y +P   FE++    +E   +D+Q
Sbjct:   521 VIDSCMKQKPGKYLLMKDPQSPVIRLYSLPEGTFESERESSDEENSDDDQ 570


to_Entrezto_Relatedto_Related >gi|8843986|gb|AAF80206.1|AF282853_5  (AF282853) CagP [Helicobacter pylori]
            Length = 110

Frame  3 hits (HSPs):    ____________________________                     
                        __________________________________________________
Database sequence:     |        |        |        |        |         |    | 110
                       0       20       40       60       80       100

  Plus Strand HSPs:

 Score = 71 (25.0 bits), Expect = 8.8, P = 1.0
 Identities = 24/61 (39%), Positives = 32/61 (52%), Frame = +3

Query:   399 LKQNFLVFFFYATAVYDLQTLAY-LSGTTKAVISFKIVLRSFGLRVIIVVYKFPCLHRVI 575
             LKQNFL F +      D  +L Y L   +  VI F I L S+G  VI+V Y    L  +I
Sbjct:     4 LKQNFLQFKYSFNKHLDKYSLYYRLFNISSIVIGFLIGLFSYGAGVILV-YPILFLFALI 62

Query:   576 LR 581
             ++
Sbjct:    63 IK 64


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.98

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.359   0.162   0.621  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.355   0.156   0.536  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.344   0.156   0.525  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.355   0.156   0.576  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.348   0.154   0.519  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.355   0.156   0.560  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      200       198       10.  76 3  12 22  0.093   35
                                                    31  0.10    38
   +2      0      200       199       10.  77 3  12 22  0.093   35
                                                    31  0.10    38
   +1      0      200       199       10.  77 3  12 22  0.093   35
                                                    31  0.10    38
   -1      0      200       198       10.  76 3  12 22  0.093   35
                                                    31  0.10    38
   -2      0      200       198       10.  76 3  12 22  0.093   35
                                                    31  0.10    38
   -3      0      200       199       10.  77 3  12 22  0.093   35
                                                    31  0.10    38


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  12
  No. of states in DFA:  595 (59 KB)
  Total size of DFA:  223 KB (256 KB)
  Time to generate neighborhood:  0.02u 0.00s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  198.73u 1.22s 199.95t  Elapsed: 00:00:34
  Total cpu time:  198.76u 1.24s 200.00t  Elapsed: 00:00:34
  Start:  Fri Jan 18 12:48:14 2002   End:  Fri Jan 18 12:48:48 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000