Isolation of a highly cytopathic lentivirus from a nondomestic cat.

J Virol. 1995 November; 69(11): 7371–7374.

PMCID: PMC189672

M C Barr, L Zou, D L Holzschu, L Phillips, F W Scott, J W Casey, and R J Avery

Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.

This article has been cited by other articles in PMC.

Abstract

A feline immunodeficiency virus-like virus (FIV-Oma) isolated from a Pallas' cat (Otocolobus manul) is highly cytopathic in CrFK cells, in contrast to the chronic, noncytolytic infection established by an FIV isolate from a domestic cat (FIV-Fca). The virions have typical lentivirus morphology, density, and magnesium-dependent reverse transcriptase activity. The major core protein is antigenically cross-reactive with that of FIV-Fca; however, FIV-Oma transcripts do not cross-hybridize with FIV-Fca. A conserved region of the FIV-Oma pol gene has 76 to 80% nucleic acid identify with the corresponding pol regions of other feline lentiviruses and 64 to 69% identity with those of human, ovine, and equine lentiviruses.

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Ackley, CD; Yamamoto, JK; Levy, N; Pedersen, NC; Cooper, MD. Immunologic abnormalities in pathogen-free cats experimentally infected with feline immunodeficiency virus. J Virol. 1990 Nov;64(11):5652–5655. [PubMed]
Barlough, JE; Ackley, CD; George, JW; Levy, N; Acevedo, R; Moore, PF; Rideout, BA; Cooper, MD; Pedersen, NC. Acquired immune dysfunction in cats with experimentally induced feline immunodeficiency virus infection: comparison of short-term and long-term infections. J Acquir Immune Defic Syndr. 1991;4(3):219–227. [PubMed]
Barr, MC; Pough, MB; Jacobson, RH; Scott, FW. Comparison and interpretation of diagnostic tests for feline immunodeficiency virus infection. J Am Vet Med Assoc. 1991 Nov 15;199(10):1377–1381. [PubMed]
Barr, SC; Bowman, DD; Phillips, LG; Barr, MC. Trypanosoma manulis n. sp. from the Russian Pallas cat Felis manul. J Eukaryot Microbiol. 1993 40(3):233–237.May–Jun; [PubMed]
Brown, EW; Yuhki, N; Packer, C; O'Brien, SJ. A lion lentivirus related to feline immunodeficiency virus: epidemiologic and phylogenetic aspects. J Virol. 1994 Sep;68(9):5953–5968. [PubMed]
Campbell, BJ; Thompson, DR; Williams, JR; Campbell, SG; Avery, RJ. Characterization of a New York ovine lentivirus isolate. J Gen Virol. 1993 Feb;74 (:201–210. [PubMed]
Carlson, JR; Yee, J; Hinrichs, SH; Bryant, ML; Gardner, MB; Pedersen, NC. Comparison of indirect immunofluorescence and Western blot for detection of anti-human immunodeficiency virus antibodies. J Clin Microbiol. 1987 Mar;25(3):494–497. [PubMed]
Chomczynski, P; Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. [PubMed]
Dow, SW; Poss, ML; Hoover, EA. Feline immunodeficiency virus: a neurotropic lentivirus. J Acquir Immune Defic Syndr. 1990;3(7):658–668. [PubMed]
English, RV; Nelson, P; Johnson, CM; Nasisse, M; Tompkins, WA; Tompkins, MB. Development of clinical disease in cats experimentally infected with feline immunodeficiency virus. J Infect Dis. 1994 Sep;170(3):543–552. [PubMed]
Fomsgaard, A; Hirsch, VM; Allan, JS; Johnson, PR. A highly divergent proviral DNA clone of SIV from a distinct species of African green monkey. Virology. 1991 May;182(1):397–402. [PubMed]
Gelman, IH; Zhang, J; Hailman, E; Hanafusa, H; Morse, SS. Identification and evaluation of new primer sets for the detection of lentivirus proviral DNA. AIDS Res Hum Retroviruses. 1992 Dec;8(12):1981–1989. [PubMed]
Hahn, BH; Shaw, GM; Arya, SK; Popovic, M; Gallo, RC; Wong-Staal, F. Molecular cloning and characterization of the HTLV-III virus associated with AIDS. Nature. 312(5990):166–169. [PubMed]
Heine, CW; Kelly, DC; Avery, RJ. The detection of intracellular retrovirus-like entities in Drosophila melanogaster cell cultures. J Gen Virol. 1980 Aug;49(2):385–395. [PubMed]
Ishida, T; Tomoda, I. Clinical staging of feline immunodeficiency virus infection. Nippon Juigaku Zasshi. 1990 Jun;52(3):645–648. [PubMed]
Kükedi, A; Bartha, A; Nagy, B. Latent infection with feline syncytial virus of cell cultures prepared from the kidneys of new-born kittens. Vet Microbiol. 1988 Jan;16(1):9–14. [PubMed]
Langley, RJ; Hirsch, VM; O'Brien, SJ; Adger-Johnson, D; Goeken, RM; Olmsted, RA. Nucleotide sequence analysis of puma lentivirus (PLV-14): genomic organization and relationship to other lentiviruses. Virology. 1994 Aug 1;202(2):853–864. [PubMed]
Levy, JA. Pathogenesis of human immunodeficiency virus infection. Microbiol Rev. 1993 Mar;57(1):183–289. [PubMed]
Lutz, H; Isenbügel, E; Lehmann, R; Sabapara, RH; Wolfensberger, C. Retrovirus infections in non-domestic felids: serological studies and attempts to isolate a lentivirus. Vet Immunol Immunopathol. 1992 Dec;35(1-2):215–224. [PubMed]
Miyazawa, T; Fukasawa, M; Hasegawa, A; Maki, N; Ikuta, K; Takahashi, E; Hayami, M; Mikami, T. Molecular cloning of a novel isolate of feline immunodeficiency virus biologically and genetically different from the original U.S. isolate. J Virol. 1991 Mar;65(3):1572–1577. [PubMed]
Miyazawa, T; Tomonaga, K; Kawaguchi, Y; Mikami, T. The genome of feline immunodeficiency virus. Arch Virol. 1994;134(3-4):221–234. [PubMed]
O'Connor, TP, Jr; Tanguay, S; Steinman, R; Smith, R; Barr, MC; Yamamoto, JK; Pedersen, NC; Andersen, PR; Tonelli, QJ. Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus). J Clin Microbiol. 1989 Mar;27(3):474–479. [PubMed]
Olmsted, RA; Barnes, AK; Yamamoto, JK; Hirsch, VM; Purcell, RH; Johnson, PR. Molecular cloning of feline immunodeficiency virus. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2448–2452. [PubMed]
Olmsted, RA; Langley, R; Roelke, ME; Goeken, RM; Adger-Johnson, D; Goff, JP; Albert, JP; Packer, C; Laurenson, MK; Caro, TM, et al. Worldwide prevalence of lentivirus infection in wild feline species: epidemiologic and phylogenetic aspects. J Virol. 1992 Oct;66(10):6008–6018. [PubMed]
Oram, JD; Downing, RG; Roff, M; Clegg, JC; Serwadda, D; Carswell, JW. Nucleotide sequence of a Ugandan HIV-1 provirus reveals genetic diversity from other HIV-1 isolates. AIDS Res Hum Retroviruses. 1990 Sep;6(9):1073–1078. [PubMed]
Pedersen, NC; Ho, EW; Brown, ML; Yamamoto, JK. Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. Science. 1987 Feb 13;235(4790):790–793. [PubMed]
Phillips, TR; Talbott, RL; Lamont, C; Muir, S; Lovelace, K; Elder, JH. Comparison of two host cell range variants of feline immunodeficiency virus. J Virol. 1990 Oct;64(10):4605–4613. [PubMed]
Saiki, RK; Gelfand, DH; Stoffel, S; Scharf, SJ; Higuchi, R; Horn, GT; Mullis, KB; Erlich, HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [PubMed]
Shelton, GH; Grant, CK; Cotter, SM; Gardner, MB; Hardy, WD, Jr; DiGiacomo, RF. Feline immunodeficiency virus and feline leukemia virus infections and their relationships to lymphoid malignancies in cats: a retrospective study (1968-1988). J Acquir Immune Defic Syndr. 1990;3(6):623–630. [PubMed]
Siebelink, KH; Chu, IH; Rimmelzwaan, GF; Weijer, K; van Herwijnen, R; Knell, P; Egberink, HF; Bosch, ML; Osterhaus, AD. Feline immunodeficiency virus (FIV) infection in the cat as a model for HIV infection in man: FIV-induced impairment of immune function. AIDS Res Hum Retroviruses. 1990 Dec;6(12):1373–1378. [PubMed]
Towbin, H; Staehelin, T; Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PubMed]
Yamamoto, JK; Pedersen, NC; Ho, EW; Okuda, T; Theilen, GH. Feline immunodeficiency syndrome--a comparison between feline T-lymphotropic lentivirus and feline leukemia virus. Leukemia. 1988 Dec;2(12 Suppl):204S–215S. [PubMed]
Yamamoto, JK; Sparger, E; Ho, EW; Andersen, PR; O'Connor, TP; Mandell, CP; Lowenstine, L; Munn, R; Pedersen, NC. Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. Am J Vet Res. 1988 Aug;49(8):1246–1258. [PubMed]