Guimarfes Lindenmeyer M, Moreira Santos A, Loureiro R, Bongertz V, Galveo Castro B, Morgado Gonsalves M; International Conference on AIDS.

Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. MoPeA2066.

M. Guimarfes Lindenmeyer, FIOCRUZ, RJ, Brazil, Av. Brazil 4365 - Pav. Carlos Chagas, Department of Immunology, - Lab. of AIDS and Molecular Immunology, CEP: 21-045-900, Brazil, Tel.: +55 055 280 1486, Fax: +55 055 280 1589, E-mail: monicklg@gene.dbbm.fiocruz.br

The aim of this study is to evaluate the polymorphism of the immunologically relevant of env and gag regions of HIV-1 Brazilian samples. And to discuss its potential relevance in terms of vaccine design. Phylogenetic studies will also performed to evaluate the presence of recombinant genomes. Proviral DNA of HIV-1 isolates from distinct Brazilian regions were typed for the gp120 C2-V3 region by Heteroduplex Mobility Assay (HMA), and for the B samples we used the RFLP with Fok I, to distinguish the variant B" as described in Morgado et al., 1998. A subset of samples corresponding to the HIV-1 subtypes prevalent in Brazil was selected for sequencing of gp120, gp41 and p17 and further phylogenetic analysis. In order to evaluate the prevalence of HIV-1 subtypes in Brazil, we typed 407 samples of HIV-1 from differents geographic regions, by HMA. From these samples 293 (72%) were included in B subtype, 38 (9,3%) in subtype F, 35 (8,6%) in subtype C, 1 in subtype D and 41 samples werent typable. From a subset of 277 subtype B samples, 86 (31%) belonged to the B" variant of HIV-1. From this data we could verify that the variant B" was more frequently found in the southwest region (37%). The subtype F (13,7%) in Southwest and subtype C (31,8%) in the South, as the second more prevalent subtype, with a highest possibility of finding recombinant samples in these regions. Data of phylogenetic analysis of 19 samples in gp120, gp41 and gag could confirm the presence of 5 possible recombinant genomes. From four subtype F samples in gp120, two recombinant samples was gp120-F/gp41-F/gag-B, one F/B/B and just one pure F. Two of three subtype C samples were C/C/B and one sample classified as B" in gp120, having the gag region from subtype C. The presence of distinct HIV-1 subtypes and the high percentual of circulating recombinant forms (CRF) found in Brazil suggests that more samples has to be analyzed to verify if CRF are representative in Brazilian samples.

Publication Types:

• Meeting Abstracts

Keywords:

• Brazil
• Genes, env
• Genes, gag
• HIV Envelope Protein gp120
• HIV-1
• Polymorphism, Restriction Fragment Length
• Prevalence
• genetics
• immunology

Other ID:

• GWAIDS0000125

UI: 102237614

From Meeting Abstracts