| Clin Exp Immunol. 1976 July; 25(1): 133–138. | PMCID: PMC1541373 |
Macrophage migration inhibition studies of lymphocytes taken from guinea-pigs suffering from experimental polymyositis. P D Smith and T A Partridge Abstract An experimental form of polymyositis, induced in guinea-pigs by means of injections of rabbit muscle homogenate in Freund's complete adjuvant (FCA), had been investigated with a view to the analysis of the role of cellular immune response in this disease. We have used the direct macrophage migration inhibition test on peritoneal exudate cells taken from myositic animals, so as to identify those components of muscle against which lymphocytes of myositic animals are sensitized. These lymphocytes were found to be sensitized principally against the myofibrillar fraction of muscle. Moreover, when tested against purified myofibrillar proteins, they were found to be strongly sensitized against myosin and tropomyosin, but not against troponin. A similar migration inhibition technique is now being developed for use as a diagnostic and investigatory tool in the study of human polymyositis. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (971K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. - Currie, S. Experimental myositis: the in-vivo and in-vitro activity of lymph-node cells. J Pathol. 1971 Nov;105(3):169–185. [PubMed]
- Dawkins, RL. Experimental myositis associated with hypersensitivity to muscle. J Pathol Bacteriol. 1965 Oct;90(2):619–625. [PubMed]
- Dawkins, RL; Eghtedari, A; Holborow, EJ. Antibodies to skeletal muscle demonstrated by immunofluorescence in experimental autoallergic myositis. Clin Exp Immunol. 1971 Sep;9(3):329–337. [PubMed]
- Dawkins, RL; Lamont, M. Myogenesis in vitro as demonstrated by immunofluorescent staining with antimuscle serum. Exp Cell Res. 1971 Jul;67(1):1–10. [PubMed]
- Dawkins, RL; Loewi, G. Cytotoxic effect of antisera on 51Cr-labelled monolayers of skeletal muscle. J Pathol. 1973 May;110(1):67–74. [PubMed]
- Esiri, MM; MacLennan, IC. Experimental myositis in rats. I. Histological and creatine phosphokinase changes, and passive transfer to normal syngeneic rats. Clin Exp Immunol. 1974 May;17(1):139–150. [PubMed]
- GEORGE, M; VAUGHAN, JH. In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962 Nov;111:514–521. [PubMed]
- Holland, DL; Perry, SV. The adenosine triphosphatase and calcium ion-transporting activities of the sarcoplasmic reticulum of developing musce. Biochem J. 1969 Sep;114(2):161–170. [PubMed]
- Kakulas, BA. In vitro destruction of skeletal muscle by sensitized cells. Nature. 1966 Jun 11;210(5041):1115–1118. [PubMed]
- Kalden, JR; Williamson, WG; Irvine, WJ. Experimental myasthenia gravis, myositis and myocarditis in guinea-pigs immunized with subcellular fractions of calf thymus or calf skeletal muscle in Freund's complete adjuvant. Clin Exp Immunol. 1973 Jan;13(1):79–88. [PubMed]
- Manghani, D; Partridge, TA; Sloper, JC. The role of myofibrillar fraction of skeletal muscle in the production of experimental polymyositis. J Neurol Sci. 1974 Dec;23(4):489–503. [PubMed]
- Morgan, G; Peter, JB; Newbould, BB. Experimental allergic myositis in rats. Arthritis Rheum. 1971 14(5):599–609.Sep–Oct; [PubMed]
- Partridge, TA; Smith, PD. A quantitative test to detect lymphocytes sensitized against the surface of muscle cells. Clin Exp Immunol. 1976 Jul;25(1):139–143. [PubMed]
- PEARSON, CM. Development of arthritis, periarthritis and periostitis in rats given adjuvants. Proc Soc Exp Biol Med. 1956 Jan;91(1):95–101. [PubMed]
- Webb, JN. Experimental immune myositis in guinea pigs. J Reticuloendothel Soc. 1970 Mar;7(3):305–316. [PubMed]
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