Lynch J, Desouza M, Robb M, Nitayaphan S, Markowitz L, Birx D, Cox J; International Conference on AIDS.
Int Conf AIDS. 1998; 12: 520 (abstract no. 602/31128).
Walter Reed Army Institute of Research, Washington DC, USA.
OBJECTIVE: To compare the frequency and magnitude of same clade and cross-clade CTL responses to four HIV-1 proteins (Gag, Pol-RT, Env and Nef) among HLA defined clade B infected North Americans and clade E infected Thais. METHODS: The CTL population (effectors) was expanded from PBMC of 10 B infected NA and 12 E infected Thais using a 14 day in vitro stimulation with cells expressing each protein. Autologous targets were created with EBV transformed B cell lines infected with vaccinia virus constructs containing HIV gene insertions. Lysis of targets was measured by Chromium release at different effector to target ratios. CTL response was measured to both homologous and cross-clade proteins. All subjects were HLA typed at the A and B loci by molecular typing techniques. RESULTS: HLA distributions for study subject North Americans (NA) and Thais were distinctly different and representative of their origin. CTL responses were detected against all proteins but were most frequent to Gag in both groups (5/10 NA, 8/9 Thais). Cross-clade Gag responses were seen in 9 of 10 responders tested. Pol-RT was recognized less frequently by Thais (2/9) than North Americans (5/10). Nef and Env were infrequently recognized by both groups. Cross clade protein recognition was relatively common for Gag, Pol-RT and Nef but rare (1/6) for the highly diverse Env. CONCLUSION: Gag is likely to be an important antigen in a broadly immunogenic vaccine. The limited cross recognition of Env suggests that inclusion of clade E Env may be prudent for a vaccine to be tested in Thailand. Variability in CTL recognition between HLA disparate populations suggests that population specific CTL data is needed to adequately prepare for vaccine trials outside of North America and Europe.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Asian Continental Ancestry Group
- Europe
- HIV Infections
- HIV Seropositivity
- HIV-1
- HLA Antigens
- In Vitro
- North America
- T-Lymphocytes, Cytotoxic
- Thailand
- Vaccinia virus
- immunology
Other ID:
UI: 102229519
From Meeting Abstracts