Bibliographic Citation
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Title | Target size of the ryanodine receptor from junctional terminal cisternae of sarcoplasmic reticulum |
Creator/Author | McGrew, S.G. ; Boucek, R.J. Jr. ; McIntyre, J.O. ; Jung, C.Y. ; Fleischer, S. |
Publication Date | 1987 Jun 02 |
OSTI Identifier | OSTI ID: 6019939 |
Other Number(s) | CODEN: BICHA |
Resource Type | Journal Article |
Resource Relation | Biochemistry ; Vol/Issue: 26:11 |
Research Org | Vanderbilt Univ., Nashville, TN |
Subject | 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture; CHOLINE-- BIOCHEMICAL REACTION KINETICS;CHOLINE-- RECEPTORS;SARCOPLASMIC RETICULUM-- BIOLOGICAL RADIATION EFFECTS; CALCIUM;CELL MEMBRANES;MOLECULAR WEIGHT;MUSCLES;RABBITS |
Related Subject | ALCOHOLS;ALKALINE EARTH METALS;AMINES;AMMONIUM COMPOUNDS;ANIMALS;BIOLOGICAL EFFECTS;CELL CONSTITUENTS;DRUGS;ELEMENTS;HYDROXY COMPOUNDS;KINETICS;LIPOTROPIC FACTORS;MAMMALS;MEMBRANE PROTEINS;MEMBRANES;METALS;ORGANIC COMPOUNDS;ORGANOIDS;PROTEINS;QUATERNARY COMPOUNDS;RADIATION EFFECTS;REACTION KINETICS;VERTEBRATES |
Description/Abstract | Target inactivation analysis was carried out on the ryanodine receptor.^This receptor recently has been implicated as the channel involved in the calcium release process in excitation-contraction coupling and was localized to the junctional terminal cisternae of sarcoplasmic reticulum from skeletal muscle.^Irradiation of the junctional terminal cisternae resulted in an exponential decrease in ryanodine binding with radiation dose, thereby consistent with target theory.^The target molecular weight was found to be 138,000 +/- 21,000, i.e., smaller than the polypeptide that binds ryanodine.^The calcium pump protein in the same membrane preparation served as an internal control to validate the methodology. |
Country of Publication | United States |
Language | English |
Format | Pages: 3183-3187 |
System Entry Date | 2001 May 13 |
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