PSI-BLAST Output: FIRST ITERATION
Query= gi|2501594|sp|Q57997|Y577_METJA PROTEIN MJ0577.
(162 letters)
Database: Non-redundant GenBank CDS
translations+PDB+SwissProt+SPupdate+PIR
448,825 sequences; 138,230,162 total letters
Interpreting the Results of the First Iteration
The results of the first iteration shown on this page include numerous new hits (designated
Legend:
Sequences producing significant alignments: 240 5e-63
209 7e-54
170 5e-42
169 9e-42
169 2e-41
161 2e-39
154 3e-37
154 4e-37
146 7e-35
145 2e-34
141 2e-33
138 3e-32
136 1e-31
135 1e-31
135 2e-31
134 4e-31
133 9e-31
128 2e-29
128 2e-29
127 4e-29
123 8e-28
122 1e-27
122 2e-27
119 9e-27
116 1e-25
113 7e-25
112 2e-24
110 8e-24
109 2e-23
106 9e-23
105 2e-22
102 2e-21
putative filament protein [Escherichia coli] 99 2e-20
Is it a filament protein? Maybe so. The E value for the alignment of the 168 aa MJ0577 with the 168 aa E. coli filament protein suggests that the alignment is highly significant. The alignment itself looks respectable. To further investigate this similarity, copy the gi number (gi 1787640) for the filament protein and paste it into a fresh PSI BLAST query page . The initial BLAST search of the filament protein retrieves relatively few significant hits and these are unannotated. The Methanococcus jannaschii MJ0531, a relative of MJ0577 comes up, but MJ0577 itself, falls below the threshold. Results of the first PSI-BLAST iteration produce the full complement of MJ0577 relatives as well as many of the hits seen using MJ0577 as the query. MJ0577 is clearly related to this protein, however, our efforts on behalf of this protein do not bring us any closer to understanding the function of MJ0577.
98 3e-20
97 1e-19
94 6e-19
91 3e-18
89 2e-17
88 4e-17
87 6e-17
86 1e-16
86 1e-16
86 2e-16
84 6e-16
83 1e-15
82 2e-15
80 8e-15
80 8e-15
79 1e-14
78 3e-14
77 7e-14
77 7e-14
76 2e-13
75 4e-13
74 5e-13
74 6e-13
74 6e-13
73 1e-12
73 1e-12
71 4e-12
71 5e-12
69 3e-11
67 8e-11
67 1e-10
67 1e-10
66 1e-10
66 2e-10
(AE000991) cationic amino acid transporter (cat-1) [A... 64 5e-10
Is it a cationic amino acid transporter ? an Na/H antiporter? Unlikely. The alignment of the relatively short MJ0577 with the 780 aa cationic amino acid transporter indicates that the MJ0577 is unlikely to be a cationic amino acid transporter itself, but that it may share a domain in common with this protein. One way to evaluate whether the shared domain is informative with respect to the function of the uncharacterized MJ0577 protein is to perform a reverse PSI-BLAST search. Copy the gi number for the cationic amino acid transporter and paste it into a fresh PSI-BLAST query page . The results of this search can be easily grasped by examining the graphic . The large number of highly significant hits (pink bars) are to amino acid transporters of various sorts. The similarity to aa transporters resides between aa 45 and aa 440, for the most part. Modest similarity to MJ0577 is localized to a separate region of the protein, aa 550 - aa 720. Therefore MJ0577 is not an amino acid transporter.
The Na(+)/H(+) antiporter from Synechocystis sp . is another annotated MJ0577 hit. Like the cationic amino acid transporter, its long length (698 aa) relative to MJ0577 suggests that these two proteins may have a domain in common but are not homologous proteins. Inspection of the alignment shows that the antiporter sequence can be aligned in two mutually exclusive ways to MJ0577, though only alignment 1 is better than the significance threshold (E = 6 x 10 -5 vs. E = 6.7). Alignment 1 involves just aa 107-153 of MJ0577 and is therefore unlikely to reveal the function of the query protein, as is borne out by doing the appropriate reverse searches.
63 1e-09
61 6e-09
60 8e-09
60 1e-08
59 2e-08
57 9e-08
56 1e-07
55 3e-07
UNIVERSAL STRESS PROTEIN A HOMOL... 54 6e-07
MJ0577 is probably a member of the Universal Stress Protein Family . The final set of significant annotated hits are to a set of proteins with similarity to the Universal stress protein (Usp) of E. coli . This similarity between individual members of the Usp family and MJ0577 is weak but the alignments are respectable. A BLAST search with the aa sequence of E. coli UspA reveals a small set of UspA homologs as the sole significant hits. In the first PSI-BLAST iteration using UspA as a query, MJ0577 and some of its closest relatives emerge as signficant hits.
Experimental Follow-Up
Inspection of the alignments of the significant annotated hits with the query, MJ0577 suggests that the best leads are the putative filament protein from E. coli and a set of universal stress proteins. The hypothesis that the MJ0577 open reading frame encodes an archaeal stress protein can be further investigated experimentally. Experiments addressing MJ0577 function have been conducted.
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54 8e-07
54 8e-07
53 1e-06
52 2e-06
52 3e-06
51 4e-06
51 4e-06
51 5e-06
51 5e-06
50 1e-05
49 2e-05
48 3e-05
UNIVERSAL STRESS PROTEIN A >gi|28... 48 3e-05
uspA protein - Escherichia coli >gi|46663... 48 3e-05
48 4e-05
Na(+)/H(+) antiporter [Synecho... 47 6e-05
UspA analogue [Salmonella ty... 45 3e-04
45 4e-04
44 7e-04
44 7e-04
44 7e-04
44 9e-04
Sequences with E-value WORSE than threshold gi|6682867|dbj|BAA88920.1| (AB023410) cyst specific protein CSP ... 43 0.001
gi|6460834|gb|AAF12538.1|AE001826_7 (AE001826) KdpD-related prot... 42 0.002
gi|2827518|emb|CAA16526.1| (AL021633) putative protein [Arabidop... 42 0.003
gi|1653666|dbj|BAA18578| (D90915) chloride channel protein [Syne... 41 0.006
gi|2804254|dbj|BAA24438| (AB010283) CSP21 [Acanthamoeba castella... 40 0.010
gi|5458909|emb|CAB50396.1| (AJ248287) PUTATIVE FLAGELLA-RELATED ... 40 0.013
gi|5748640|emb|CAB53145.1| (AL109962) hypothetical protein SCJ1.... 39 0.029
gi|4337196|gb|AAD18110.1| (AC006403) putative protein kinase [Ar... 39 0.029
gi|84186|pir||JN0292 antigen 332 - Plasmodium falciparum (fragme... 38 0.038
gi|2495365|sp|P55737|HS82_ARATH HEAT SHOCK PROTEIN 81-2 (HSP81-2... 38 0.050
gi|3913894|sp|O67825|IF2_AQUAE TRANSLATION INITIATION FACTOR IF-... 37 0.065
gi|160034 (M69161) antigen 332 [Plasmodium falciparum] 37 0.065
gi|246924|bbs|86426 antigen 332, Ag332=Pf332 gene clone G9 produ... 37 0.065
gi|3152590 (AC002986) Similar to protein serine/threonine kinase... 37 0.085
gi|3256959|dbj|BAA29642| (AP000002) 399aa long hypothetical prot... 37 0.085
gi|6635817|gb|AAF19990.1|AF213466_5 (AF213466) potassium-depende... 36 0.15
gi|5104423|dbj|BAA79738.1| (AP000060) 142aa long hypothetical pr... 36 0.15
gi|1708313|sp|P51818|HS83_ARATH HEAT SHOCK PROTEIN 81-3 (HSP81-3... 36 0.15
gi|1906828|emb|CAA72514| (Y11828) heat shock protein [Arabidopsi... 36 0.19
gi|1226269 (U50301) Similar to oxidoreductase [Caenorhabditis el... 36 0.19
gi|3122321|sp|P96372|KDPD_MYCTU SENSOR PROTEIN KDPD >gi|1870000|... 35 0.25
gi|2120481|pir||I40130 outer surface protein G - Lyme disease sp... 35 0.25
gi|2120431|pir||S70533 bbK2.10 protein precursor - Lyme disease ... 35 0.25
gi|5825477|gb|AAD53262.1|AF149296_1 (AF149296) hard-surface indu... 35 0.33
gi|1790450 (AE000475) B12-dependent homocysteine-N5-methyltetrah... 35 0.33
gi|1065138|pdb|1BMT|A Escherichia coli >gi|1065139|pdb|1BMT|B Es... 35 0.33
gi|2133786|pir||I51116 NF-180 - sea lamprey >gi|632549 (U19361) ... 35 0.33
gi|581135|emb|CAA34601| (X16584) 5-methyltetrahydrofolate- homoc... 35 0.33
gi|409794 (U00006) B12-dependent homocysteine-N5-methyltetrahydr... 35 0.33
gi|400244|sp|P13009|METH_ECOLI 5-METHYLTETRAHYDROFOLATE--HOMOCYS... 35 0.33
gi|228477|prf||1804350B ECLF2 upstream ORF [saimirine herpesviru... 35 0.43
gi|266334|sp|Q01042|IE68_HSVSA IMMEDIATE-EARLY PROTEIN >gi|73643... 35 0.43
gi|5123910|emb|CAA67191.1| (X98582) HSP80-2 [Triticum aestivum] 34 0.56
gi|2129444|pir||S63531 hypothetical protein 1 - Sulfolobus solfa... 34 0.56
gi|1945336|emb|CAA97193| (Z72953) ORF YGR167w [Saccharomyces cer... 34 0.56
gi|1707290 (U80960) putative outer surface protein [Borrelia bur... 34 0.56
gi|1235605|emb|CAA56461| (X80178) urf2 [Sulfolobus solfataricus] 34 0.56
gi|5453762|ref|NP_006149.1|| neurofilament, light polypeptide (6... 34 0.56
gi|417154|sp|P33126|HS82_ORYSA HEAT SHOCK PROTEIN 82 >gi|100685|... 34 0.56
gi|6321606|ref|NP_011683.1|CLC1| Clathrin light chain; Clc1p >gi... 34 0.56
gi|84211|pir||S00485 gene 11-1 protein precursor - Plasmodium fa... 34 0.56
gi|9826|emb|CAA30336| (X07453) 11-1 polypeptide [Plasmodium falc... 34 0.56
gi|2094830|emb|CAB08583| (Z95324) grpE [Mycobacterium tuberculosis] 34 0.74
gi|417087|sp|P32724|GRPE_MYCTU GRPE PROTEIN (HSP-70 COFACTOR) >g... 34 0.74
gi|547683|sp|P36181|HS80_LYCES HEAT SHOCK COGNATE PROTEIN 80 >gi... 33 0.97
Combined sequence, structural and biochemical studies indicate that MJ0577, its paralog, MJ0533, and a number of orthologs in other archael and eubacterial species are members of the Universal Stress Protein Family. Expression of the Universal stress protein in E. coli is induced at the transcriptional level under conditions of stress that lead to growth inhibition or arrest. Mutants are impaired in their ability to survive prolonged periods of complete growth inhibition caused by a variety of diverse stresses, including CdCl2, H2 O2 ,and osmotic shock. uspA mutants are also sensitive to carbon-source starvation (i.e. depletion of glucose, glycerol or succinate)and exhibit alterations in the timing of starvation protein expression. UspA and UspA like proteins are therefore proposed to have a general protective function related to the growth arrest state. Computational analysis has revealed similarity between the N terminus of the UspA family of proteins and the DNA binding domain of the eukaryotic MADS box transcription factor family (Mushegian and Koonin ). This has led to the hypothesis that UspA family members may function by up-regulating stress response proteins at the transcriptional level. This idea can now be tested experimentally. What role ATP binding and phosphorylation play in the predicted functions of Usp proteins can be readily assessed by directed mutagenesis.
iteration 2 and convergence at which point no additional new sequences are detected, can be examined here, however, in this case, no further insights about MJ0577 function emerged from this analysis.
Alignments
sp|Q57997|Y577_METJA PROTEIN MJ0577 >gi|2128018|pir||A64372 hypothetical protein homolog
MJ0577 - Methanococcus jannaschii >gi|5107801|pdb|1MJH|A
Chain A, Structure-Based Assignment Of The Biochemical
Function Of Hypothetical Protein Mj0577: A Test Case Of
Structural Genomics >gi|5107802|pdb|1MJH|B Chain B,
Structure-Based Assignment Of The Biochemical Function
Of Hypothetical Protein Mj0577: A Test Case Of
Structural Genomics >gi|1591284 (U67506) conserved
hypothetical protein [Methanococcus jannaschii]
Length = 162
Score = 240 bits (606), Expect = 5e-63
Identities = 162/162 (100%), Positives = 162/162 (100%)
Query: 1 MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA
Sbjct: 1 MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG 120
GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG
Sbjct: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG 120
Query: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS
Sbjct: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
pdb|1MJH| Structure-Based Assignment Of The Biochemical Function Of
Hypothetical Protein Mj0577: A Test Case Of Structural
Genomics
Length = 287
Score = 209 bits (528), Expect = 7e-54
Identities = 145/161 (90%), Positives = 145/161 (90%), Gaps = 16/161 (9%)
Query: 2 SVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAG 61
SVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREI
Sbjct: 143 SVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREI-------------- 188
Query: 62 LNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV 121
KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV
Sbjct: 189 --KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV 246
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS
Sbjct: 247 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 287
Score = 206 bits (520), Expect = 6e-53
Identities = 143/160 (89%), Positives = 143/160 (89%), Gaps = 17/160 (10%)
Query: 3 VMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGL 62
VMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIK
Sbjct: 1 VMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIK-------------- 46
Query: 63 NKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVD 122
VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVD
Sbjct: 47 ---VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVD 103
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS
Sbjct: 104 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 143
sp|Q57951|Y531_METJA HYPOTHETICAL PROTEIN MJ0531 >gi|2128015|pir||C64366 hypothetical
protein homolog MJ0531 - Methanococcus jannaschii
>gi|1591234 (U67502) conserved hypothetical protein
[Methanococcus jannaschii]
Length = 170
Score = 170 bits (427), Expect = 5e-42
Identities = 59/158 (37%), Positives = 88/158 (55%), Gaps = 14/158 (8%)
Query: 3 VMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGL 62
+YKKI+ PTD S+ + A KH EV ++V+D S +G+
Sbjct: 24 NLYKKIVIPTDGSDVSLEAAKHAINIAKEFDAEVYAIYVVDV---------SPFVGLPA- 73
Query: 63 NKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVD 122
+ E +EL L EE + ++ +KK E+ G K+ ++ G+P EIV+ AE + D
Sbjct: 74 -EGSWELISEL---LKEEGQEALKKVKKMAEEWGVKIHTEMLEGVPANEIVEFAEKKKAD 129
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
+I+MG+ GKT L+ ILLGSV E VIK ++ PVLVVK+
Sbjct: 130 LIVMGTTGKTGLERILLGSVAERVIKNAHCPVLVVKKP 167
sp|P42297|YXIE_BACSU HYPOTHETICAL 15.9 KD PROTEIN IN BGLH-WAPA INTERGENIC REGION
PRECURSOR >gi|603780|dbj|BAA06654| (D31856) hypothetical
protein [Bacillus subtilis] >gi|849027|dbj|BAA06258|
(D29985) hypothetical 15.9-kDa protein [Bacillus
subtilis] >gi|2636471|emb|CAB15961| (Z99124) similar to
hypothetical proteins [Bacillus subtilis]
Length = 148
Score = 169 bits (425), Expect = 9e-42
Identities = 47/155 (30%), Positives = 83/155 (53%), Gaps = 7/155 (4%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M+ K+L D S+ + AL + E+ +LHV E + + G+
Sbjct: 1 MFNKMLVAIDGSDMSAKALDAAVHLAKEQQAELSILHVGREAVVTTSSL-------TGIV 53
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
E F +E++N++ +E +EN K++ + G + + I G P EI+ A+++GV +
Sbjct: 54 YVPEHFIDEIRNEVKKEGLKILENAKEKAAEKGVQAETIYANGEPAHEILNHAKEKGVSL 113
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
I++GS G + LKE++LGSV+ V + S PVL+V+
Sbjct: 114 IVVGSRGISGLKEMMLGSVSHKVSQLSTCPVLIVR 148
dbj|BAA29916| (AP000003) 170aa long hypothetical protein [Pyrococcus horikoshii]
Length = 170
Score = 169 bits (423), Expect = 2e-41
Identities = 63/160 (39%), Positives = 97/160 (60%), Gaps = 7/160 (4%)
Query: 1 MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
M M++K+L+PTDFSE A A++ + ++ EVILLHVIDE +++ L+ G +
Sbjct: 1 MIFMFRKVLFPTDFSEGAYRAVEVFEKRNKMEVGEVILLHVIDEGTLEE-----LMDGYS 55
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDV--GFKVKDIIVVGIPHEEIVKIAED 118
+ E ++K KL EEA K++ +E++ V+ II GIP +EIVK+AE+
Sbjct: 56 FFYDNAEIELKDIKEKLKEEASRKLQEKAEEVKRAFRAKNVRTIIRFGIPWDEIVKVAEE 115
Query: 119 EGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
E V +II+ S GK +L LGS V++K+ KPVL++K
Sbjct: 116 ENVSLIILPSRGKLSLSHEFLGSTVMRVLRKTKKPVLIIK 155
gi|2622094 (AE000872) conserved protein [Methanobacterium thermoautotrophicum]
Length = 143
Score = 161 bits (405), Expect = 2e-39
Identities = 56/156 (35%), Positives = 81/156 (51%), Gaps = 16/156 (10%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MY KIL PTD S+ A A +H E+I L V++ S L+G+
Sbjct: 1 MYSKILLPTDGSKQANKAAEHAIWIARESGAEIIALTVMET---------SSLVGLPA-- 49
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIV--VGIPHEEIVKIAEDEGV 121
++ L+ L EEA +E +KK +E+ G +K + G P E I++ E EGV
Sbjct: 50 ---DDLIIRLREMLEEEASRSLEAVKKLVEESGADIKLTVRTDEGSPAEAILRTVEKEGV 106
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
D+++MG+ GK L LLGSV E V++ + PVLVV
Sbjct: 107 DLVVMGTSGKHGLDRFLLGSVAEKVVRSAGCPVLVV 142
gi|2621993 (AE000865) conserved protein [Methanobacterium thermoautotrophicum]
Length = 149
Score = 154 bits (386), Expect = 3e-37
Identities = 56/157 (35%), Positives = 85/157 (53%), Gaps = 12/157 (7%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MY+KIL TD SE + A + E++ L V + + L V L
Sbjct: 1 MYRKILLATDGSECSMQAAGYAIETAAQNRAELLALTVTETYPLDN-------LPVEELT 53
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ V EL K +EEA K+E++ L D KV+ ++V G P E I+K+A++E VD+
Sbjct: 54 RKV----TELFRKESEEALQKVEDLAVSL-DTPVKVRKMMVDGSPAETILKVADEENVDL 108
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
I++G+ GK L+ LLGSV+E +++ + PVLVV K
Sbjct: 109 IVVGASGKHALERFLLGSVSEKIVRHARVPVLVVHSK 145
gb|AAF26483.1|AC016447_6 (AC016447) unknown protein [Arabidopsis thaliana]
Length = 160
Score = 154 bits (385), Expect = 4e-37
Identities = 46/160 (28%), Positives = 78/160 (48%), Gaps = 8/160 (5%)
Query: 2 SVMYKKILYPTDFSETAEIALKHVKA-FKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
SVM K+++ D SE ++ AL+ K A+ I+L + + +++ G A
Sbjct: 7 SVM-KQVMVAIDESECSKRALQWTLVYLKDSLADSDIILFTA-QPHLDLSCVYASSYGAA 64
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG 120
+ E N L+ N+++ K + G + ++ G P E I + AE G
Sbjct: 65 PI-----ELINSLQESHKNAGLNRLDEGTKICAETGVTPRKVLEFGNPKEAICEAAEKLG 119
Query: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
VD++++GSHGK L+ LGSV+ + + PVLVV+ K
Sbjct: 120 VDMLVVGSHGKGALQRTFLGSVSNYCVNNAKCPVLVVRTK 159
gi|2621194 (AE000803) conserved protein [Methanobacterium thermoautotrophicum]
Length = 131
Score = 146 bits (366), Expect = 7e-35
Identities = 47/155 (30%), Positives = 80/155 (51%), Gaps = 24/155 (15%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M++KI+ PTD SE A A VI +HVIDE+ I D+
Sbjct: 1 MFEKIMVPTDGSEYAARAEDMAIELAGRLGSVVIAVHVIDEKLIYPFDV----------- 49
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
L +E K + +++++ + G +V +++V G P ++ KI E G D+
Sbjct: 50 -------------LEDEGKEILASVQRKGREAGVQVDEVLVFGSPAHDMKKITEKTGADL 96
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+++ SHG++ L+++L+GSV E +K + PVL+VK
Sbjct: 97 VVIASHGRSGLEKLLMGSVAETTLKTVDVPVLLVK 131
sp|P74148|YD88_SYNY3 HYPOTHETICAL 17.3 KD PROTEIN SLL1388 >gi|1653320|dbj|BAA18235|
(D90912) hypothetical protein [Synechocystis sp.]
Length = 154
Score = 145 bits (362), Expect = 2e-34
Identities = 41/156 (26%), Positives = 74/156 (47%), Gaps = 9/156 (5%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
Y KIL D SE A+ L+ A ++ ++++ + I I+ G A +
Sbjct: 3 YGKILVALDRSELAKEVLQQAIALGQKESSQLMVFYCIPVDSQDLS-IYPSFYGEAAIG- 60
Query: 65 SVEEFENELKNKLTE---EAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV 121
F +K L E EA+ +++I +++++ G + + VG P I +A++
Sbjct: 61 ----FSQIIKEHLEEQQTEAREWLQSIVQQVQEDGVACEWDVKVGEPGRWIRDMAKNWDA 116
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
D++++G G L E+ LGSV+ VI VL+V
Sbjct: 117 DLVVLGRRGLKGLAEVFLGSVSSYVIHHVQCSVLIV 152
gi|2622163 (AE000877) conserved protein [Methanobacterium thermoautotrophicum]
Length = 147
Score = 141 bits (353), Expect = 2e-33
Identities = 48/161 (29%), Positives = 83/161 (50%), Gaps = 22/161 (13%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MY++IL PTD S A A +H + +++ + V+D K D
Sbjct: 1 MYRRILIPTDGSGDARKATRHAFHIAGMSGADILAISVVDTSYRKIWD------------ 48
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKEL---EDVGFKVKD----IIVVGIPHEEIVKIA 116
E+ L+ L ++A+ + +K+E +++G + +I+ G P E I+++
Sbjct: 49 ---EDISRRLEEILKKQAEKAISILKEEFSSQQELGHMTETRLDTVILEGNPAEVILEVM 105
Query: 117 EDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
EDE VD+++MGS GK L I+ GS+T V+K + KP++VV
Sbjct: 106 EDEDVDLVVMGSSGKHGLDRIISGSITRKVLKSATKPMMVV 146
gi|2160182 (AC000132) ESTs gb|ATTS1236,gb|T43334,gb|N97019,gb|AA395203 come
from this gene. [Arabidopsis thaliana]
Length = 174
Score = 138 bits (344), Expect = 3e-32
Identities = 37/164 (22%), Positives = 68/164 (40%), Gaps = 15/164 (9%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEE----VILLHVIDEREIKK-----RDIFS--LL 56
++ D SE + AL+ L + ++LHV + F
Sbjct: 10 VVVAVDGSEVSMEALRWALDNLKLSSSSSDSSFVVLHVQPSPSVAAGVSPGTIPFGGPSG 69
Query: 57 LGVAGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIA 116
L V ++E+ + + + + E A I E VK +V+G P +I +
Sbjct: 70 LEVPAFTAAIEQHQKRITDTILEHASQ----ICAEKSVSRVNVKTQVVIGDPKYKICEAV 125
Query: 117 EDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
E+ D+++MGS +K + LGSV+ ++ PV+++K K
Sbjct: 126 ENLHADLLVMGSRAYGRIKRMFLGSVSNYCTNHAHCPVVIIKPK 169
sp|O27222|YB54_METTH HYPOTHETICAL PROTEIN MTH1154 >gi|2622260 (AE000885) conserved
protein [Methanobacterium thermoautotrophicum]
Length = 146
Score = 136 bits (339), Expect = 1e-31
Identities = 50/163 (30%), Positives = 84/163 (50%), Gaps = 22/163 (13%)
Query: 1 MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
M MY+KIL PT E + ++H + EVI L+V+D V
Sbjct: 1 MIEMYRKILVPT-MGEYMDELIEHTLDLLHGREAEVICLYVVDTA-------------VP 46
Query: 61 GLN-KSVEEFENELKNKLTEEAKNKMENIKKEL---EDVGFKVKDIIVVGIPHEEIVKIA 116
L K V+E + +LT+ + +++K L E+ + ++ G P +EIVK+A
Sbjct: 47 FLTPKKVKEM---MVKELTQRGNEILRDMEKGLTGPENPNVSFRAVMREGDPADEIVKVA 103
Query: 117 EDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
E+E VD+I+MG+ GK+ + + LLGSV+E V+ + + +V+
Sbjct: 104 EEEDVDVIVMGT-GKSLVDKHLLGSVSEKVVHYAPCTIHLVRT 145
emb|CAB50594.1| (AJ248288) hypothetical protein [Pyrococcus abyssi]
Length = 149
Score = 135 bits (338), Expect = 1e-31
Identities = 46/156 (29%), Positives = 77/156 (48%), Gaps = 12/156 (7%)
Query: 7 KILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSV 66
K+L D S+ ++ A H + +VIL V+D RE + F L + L K +
Sbjct: 2 KLLVLIDGSKWSQKAALHAFSIAKRSNAKVILFSVLDRREARAL-AFHLSMRSESLEK-I 59
Query: 67 EEFEN----ELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVD 122
EFE ++K + E +E K+E + FK+ + G EEI+K A D
Sbjct: 60 REFEETIWKDMKKSVKEVITTLLELGKREGVNCSFKIAE----GSAKEEILKEANSGKYD 115
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
++IMG++G++ I GS+ E V+ + PV++V+
Sbjct: 116 MVIMGAYGRSGKTRI--GSLLEEVVGQIRIPVMIVR 149
dbj|BAA31039.1| (AP000007) 149aa long hypothetical protein [Pyrococcus horikoshii]
Length = 149
Score = 135 bits (337), Expect = 2e-31
Identities = 49/156 (31%), Positives = 77/156 (48%), Gaps = 12/156 (7%)
Query: 7 KILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSV 66
K+L D S+ ++ A H + K +VIL V+D RE K F L + L K +
Sbjct: 2 KLLVLIDGSKWSQKAALHAFSIAKRKNAKVILFSVLDRREAKAL-AFHLSMRSDSLGK-I 59
Query: 67 EEFEN----ELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVD 122
+EFE E K + E +E ++E + FK IV G EEI+K A
Sbjct: 60 KEFEETIWRETKKSVKEVITTLLELGRREGINCSFK----IVEGSAKEEIIKEANSGKYS 115
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
++IMG++G++ I GS+ E V+ + PV++V+
Sbjct: 116 MVIMGAYGRSGKTRI--GSLLEEVVGQIRIPVMIVR 149
sp|Q50777|YB54_METTM HYPOTHETICAL 16.1 KD PROTEIN IN MTR REGION (ORF143)
>gi|1296939|emb|CAA66198| (X97589) ORF143
[Methanobacterium thermoautotrophicum]
Length = 143
Score = 134 bits (334), Expect = 4e-31
Identities = 53/160 (33%), Positives = 86/160 (53%), Gaps = 22/160 (13%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MY+KIL PT E + ++H + EVI L+V+D V L
Sbjct: 1 MYRKILVPT-MGEYMDELIEHTLDLLHGREAEVICLYVVDT-------------SVPFLT 46
Query: 64 -KSVEEFENELKNKLTEEAKNKMENIKKEL---EDVGFKVKDIIVVGIPHEEIVKIAEDE 119
K V+E + +LTE K + +++K L E+ K + +++ G P +EIVK+AE+E
Sbjct: 47 PKKVKEM---MVKELTERGKEILRDMEKGLTGPENPNVKFRGVMLEGNPADEIVKLAEEE 103
Query: 120 GVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
VD+IIMG+ GK+ + + LLGSV+E V+ + + +V+
Sbjct: 104 DVDVIIMGT-GKSLVDKHLLGSVSEKVVHYAPCTIHLVRT 142
gb|AAF26101.1|AC012328_4 (AC012328) unknown protein [Arabidopsis thaliana]
Length = 159
Score = 133 bits (331), Expect = 9e-31
Identities = 43/158 (27%), Positives = 74/158 (46%), Gaps = 10/158 (6%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
+ + D+S T+++AL+ + VIL+HV + R I G +
Sbjct: 5 RTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGSPLI--P 62
Query: 66 VEEFENELKNKL-----TEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG 120
+EEF +K E + ++ + + + KV + G P E++ E+
Sbjct: 63 LEEFREVNLSKQYGLAYDPEVLDVLDTLSRAKK---VKVVAKVYWGDPREKLCDAVENLK 119
Query: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+D I++GS G +LK ILLGSV+ +V+ + PV VVK
Sbjct: 120 LDSIVLGSRGLGSLKRILLGSVSNHVVTNATCPVTVVK 157
gi|2983527 (AE000719) hypothetical protein [Aquifex aeolicus]
Length = 281
Score = 128 bits (320), Expect = 2e-29
Identities = 49/158 (31%), Positives = 78/158 (49%), Gaps = 14/158 (8%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLL--LGV--- 59
+ +I+ D S + IA +H VI ++VIDER + + + L LG
Sbjct: 3 FNRIIVGIDGSPASLIATRHAFKIGKHFDIPVIGMYVIDERLMDESFLLDLSSILGFTFY 62
Query: 60 AGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDE 119
G++ V+EF L E+ ++ +E G KV + V GIP +EIVK A+ E
Sbjct: 63 PGISARVKEF-------LEEQGDLILKTFAEEGRKEGVKVSIVQVQGIPWQEIVKEADKE 115
Query: 120 GVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
D+I++G GK +K +L+ S ENV + + PV +
Sbjct: 116 --DLILIGKKGKKLIKGVLVSSNAENVARNAPCPVFMF 151
Score = 85.7 bits (209), Expect = 2e-16
Identities = 41/156 (26%), Positives = 69/156 (43%), Gaps = 36/156 (23%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
KK+ D E ++ AL+ +A K E+ +L V++ +
Sbjct: 159 KKVCVAYDGKENSKRALEISRALKEPYGYEIYVLSVVE---------------------N 197
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIII 125
+EE + EE K + +E G K GIP E IV ++ +D +
Sbjct: 198 LEEAKKR-----EEEVKEVLG---EEHHFYGIK-------GIPEEVIVSFCREKEMDALF 242
Query: 126 MGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKN 161
MG++GK ++E LGSVT V+ + P+L+V++ N
Sbjct: 243 MGAYGKGPVREFFLGSVTTYVMHNLDLPLLLVRQPN 278
sp|P72817|YG54_SYNY3 HYPOTHETICAL 16.8 KD PROTEIN SLL1654 >gi|1651906|dbj|BAA16832|
(D90901) hypothetical protein [Synechocystis sp.]
Length = 157
Score = 128 bits (320), Expect = 2e-29
Identities = 43/156 (27%), Positives = 65/156 (41%), Gaps = 21/156 (13%)
Query: 3 VMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGL 62
+M+K IL+P D S A A + V + ++ILL V++
Sbjct: 21 IMFKTILFPLDRSREARDAAQMVADLVKIHQSQLILLSVVE------------------- 61
Query: 63 NKSVEEFENELKNKLTEEAKNKM-ENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV 121
K+ ++E + EA K+ E + G K I G+ I +A++
Sbjct: 62 -KNPPGQDHEAHGMDSPEAVAKLLEAAQAVFSQQGIATKTIEREGMASFTICDVADEVNA 120
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
D+I+MG G E + SVT VI S PVLVV
Sbjct: 121 DLIVMGCRGLGLTTEGVAESVTARVINLSPCPVLVV 156
sp|P74897|YQA3_THEAQ HYPOTHETICAL 14.6 KD PROTEIN IN QAH/OAS SULFHYDRYLASE 3'REGION
>gi|1526550|dbj|BAA13428| (D87664) hypothetical protein
[Thermus aquaticus]
Length = 137
Score = 127 bits (317), Expect = 4e-29
Identities = 40/156 (25%), Positives = 70/156 (44%), Gaps = 20/156 (12%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M+K IL D S+ A+ A KA ++++H + + F
Sbjct: 1 MFKTILLAYDGSDHAKRAAAVAKAEAQAHGARLLVVHAYEPVPDYLGEPF---------- 50
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVV-GIPHEEIVKIAEDEGVD 122
FE LK +L K + E + G +D +++ G P E I++ A E D
Sbjct: 51 -----FEEALKRRLERAEKVRAEAMAL----TGVPREDALLLQGRPAEAILQAAIGEKAD 101
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+I+MG+ G + + LGS ++ V+ ++ PVL+V+
Sbjct: 102 LIVMGTRGLGAVGSLFLGSQSQKVVAEAPCPVLLVR 137
sp|P39177|UP12_ECOLI UNKNOWN PROTEIN FROM 2D-PAGE (SPOTS PR25/LM16/2D_000LR3)
>gi|1778525 (U82598) hypothetical protein [Escherichia
coli] >gi|1786824 (AE000166) orf, hypothetical protein
[Escherichia coli] >gi|4062223|dbj|BAA35237| (D90701)
Unknown protein from 2D-page (spots pr25/lm16/2d_000lr3)
. [Escherichia coli] >gi|4062229|dbj|BAA35246| (D90702)
Unknown protein from 2D-page (spots pr25/lm16/2d_000lr3)
. [Escherichia coli]
Length = 142
Score = 123 bits (306), Expect = 8e-28
Identities = 42/157 (26%), Positives = 76/157 (47%), Gaps = 17/157 (10%)
Query: 4 MYKKILYPTDFSET--AEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAG 61
MYK I+ P D E ++ A++H + + LLHV+ S L +
Sbjct: 1 MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDG-VIHLLHVLPG---------SASLSLHR 50
Query: 62 LNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV 121
V FE L++ EA+ +++ + ++K + G +E+ ++AE+ G
Sbjct: 51 FAADVRRFEEHLQH----EAQERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAEELGA 106
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
D++++GS ++ LLGS +VI+ +N PVLVV+
Sbjct: 107 DVVVIGSR-NPSISTHLLGSNASSVIRHANLPVLVVR 142
sp|P45680|YFMU_COXBU HYPOTHETICAL 15.8 KD PROTEIN IN FMU-RPMH INTERGENIC REGION
>gi|2126364|pir||I40650 hypothetical protein 146 -
Coxiella burnetii >gi|511455 (U10529) unknown [Coxiella
burnetii]
Length = 146
Score = 122 bits (304), Expect = 1e-27
Identities = 44/159 (27%), Positives = 75/159 (46%), Gaps = 17/159 (10%)
Query: 5 YKKILYPTDFSETAEIAL-KHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
YKKIL ++ L + K + ++ L+H ++ + GVA
Sbjct: 4 YKKILVALALDPNSDRPLVEKAKELSANRDAQLYLIHAVE-----HLSSYGAAYGVAA-- 56
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
++++ L EEAK +M I +L I+ VG I++ A++ GVD+
Sbjct: 57 ------GVDVEDMLLEEAKKRMNEIASQLNISSDH--QIVKVGPAKFLILEQAKNWGVDL 108
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
II+GSHG+ + ++LLGS + V+ + VL V+ K S
Sbjct: 109 IIVGSHGRHGI-QLLLGSTSNAVLHGAKCDVLAVRIKGS 146
gb|AAF11689.1|AE002048_9 (AE002048) hypothetical protein [Deinococcus radiodurans]
Length = 150
Score = 122 bits (303), Expect = 2e-27
Identities = 40/154 (25%), Positives = 70/154 (44%), Gaps = 16/154 (10%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
KKIL TD S+ A +H +A E++ L V + F + +
Sbjct: 2 KKILVTTDQSDLGWQATEHARALAEALGAELVALSVQADPSPAVTGEFGYVAP-----AN 56
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIP-HEEIVKIAEDEGVDII 124
E+F + L + K++ + +E G P I+ +A++EGV +I
Sbjct: 57 PEDFILQQDQALAL-LRQKVQGARSRVERAA---------GRPVSRTIIDVAKEEGVSMI 106
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+M +HG+ L LLGSV E V+ ++ PV++++
Sbjct: 107 VMTTHGRAGLGRALLGSVAEAVLHHAHVPVVLIR 140
gb|AAD46412.1|AF096262_1 (AF096262) ER6 protein [Lycopersicon esculentum]
Length = 168
Score = 119 bits (297), Expect = 9e-27
Identities = 34/167 (20%), Positives = 60/167 (35%), Gaps = 23/167 (13%)
Query: 8 ILYPTDFSETAEIALKHVKAFKT---------LKAEEVILLHVIDEREIKKRD-----IF 53
++ D SE + AL +++LHV I F
Sbjct: 6 VIVSVDGSEESMNALNWTLDNIKLKPHDPDSPESQGFIVILHVQSPPSIAAGLNPGAIPF 65
Query: 54 S--LLLGVAGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEE 111
+ V ++E + + + + A VK +V+G P E+
Sbjct: 66 GGPSDVEVPAFTAAIEAHQKRITQAILDHALGI-------CAKKNANVKTQVVIGDPKEK 118
Query: 112 IVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
I E+ D+++MGS +K + LGSV+ + PV++VK
Sbjct: 119 ICDAVEEMNADLLVMGSRAFGPIKRMFLGSVSNYCTNHAQCPVIIVK 165
gi|2983400 (AE000710) hypothetical protein [Aquifex aeolicus]
Length = 297
Score = 116 bits (288), Expect = 1e-25
Identities = 37/155 (23%), Positives = 76/155 (48%), Gaps = 18/155 (11%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
KK++ DFS+TA+ + F V ++HV + E+
Sbjct: 156 KKVVIAYDFSKTAQKTAEFALKFLKNFKVSVEIVHVHESIEMPL---------------- 199
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVK--IAEDEGVDI 123
+E+ +++++ + +EE K + +K E+ G K + + G +++ + E V++
Sbjct: 200 IEKLKHKIEKEFSEEKKKILNELKGRFEEEGIKTEVKFLEGEDAVDVISSYVNETPEVEL 259
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+I+GS G + LK+++LG ++ K NKP+L+ K
Sbjct: 260 LIIGSKGLSGLKKLILGRTATKLLGKVNKPILIYK 294
Score = 100 bits (247), Expect = 7e-21
Identities = 48/158 (30%), Positives = 78/158 (48%), Gaps = 10/158 (6%)
Query: 1 MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
M M K L P DFSE L+ VK EV LLHVI + +GV+
Sbjct: 1 MLTM--KFLVPVDFSEITNPLLRTVKRVGEKVDCEVHLLHVIPPV---LYLPYPETMGVS 55
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG 120
++ E +L+++ EAK K++ +++ L+ K + + VG P + I+ E
Sbjct: 56 VIDI---ELLEKLEDEKKAEAKEKLKALEEFLKP--VKARSHVDVGDPADVILDYEEKLN 110
Query: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
D++ +G H K ++++L+GS TE V+K K V+K
Sbjct: 111 PDMVFLGGHKKGLIEKLLIGSTTEKVVKHGKKSDFVIK 148
gb|AAF23209.1|AC016795_22 (AC016795) unknown protein [Arabidopsis thaliana]
Length = 200
Score = 113 bits (281), Expect = 7e-25
Identities = 32/165 (19%), Positives = 66/165 (39%), Gaps = 12/165 (7%)
Query: 6 KKILYPTDFSETAEIALKHVKA----------FKTLKAEEVILLHVIDEREIKKRDIFSL 55
K+++ D S+++ AL+ V ++ + ++HV + F
Sbjct: 33 KRMVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHV--QSPFNHFAAFPA 90
Query: 56 LLGVAGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKI 115
G A + +K E + + + + + +++ G E I +
Sbjct: 91 GPGGATAVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICEA 150
Query: 116 AEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
E VD++++GS G +K LGSV++ +N P+L+VK
Sbjct: 151 VEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPP 195
gi|2648791 (AE000981) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 270
Score = 112 bits (278), Expect = 2e-24
Identities = 45/153 (29%), Positives = 78/153 (50%), Gaps = 18/153 (11%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
+L PTD SE + L+++ FK + EE+ +L VI+ ++ ++
Sbjct: 1 MLLPTDLSENSFKVLEYLGDFKKVGVEEIGVLFVINLTKLSTVSG----------GIDID 50
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDI--IVVGIPHEEIVKIAEDEGVDIII 125
+ +E+ +E+A+ + + +++E G K + I G P EI+K +E+ I
Sbjct: 51 HYIDEM----SEKAEEVLPEVAQKIEAAGIKAEVIKPFPAGDPVVEIIKASEN--YSFIA 104
Query: 126 MGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
MGS G + K+ILLGSV+E V+ S PV + K
Sbjct: 105 MGSRGASKFKKILLGSVSEGVLHDSKVPVYIFK 137
Score = 93.1 bits (228), Expect = 1e-18
Identities = 36/156 (23%), Positives = 67/156 (42%), Gaps = 34/156 (21%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
++ ++L DFS+ A+ AL++ K E+ ++HV ++ +
Sbjct: 145 LFDRVLVAYDFSKWADRALEYAKFVVKKTGGELHIIHVSEDGD----------------- 187
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ +++ + G +V I G PH+ I+ E+
Sbjct: 188 -----------------KTADLRVMEEVIGAEGIEVHVHIESGTPHKAILAKREEINATT 230
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
I MGS G ++ ++LGS +E+VI++S PV V KR
Sbjct: 231 IFMGSRGAGSVMTMILGSTSESVIRRSPVPVFVCKR 266
dbj|BAA16707| (D90900) hypothetical protein [Synechocystis sp.]
Length = 284
Score = 110 bits (272), Expect = 8e-24
Identities = 38/155 (24%), Positives = 64/155 (40%), Gaps = 23/155 (14%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M KILY + + LK + F ++ + +LHV+ + +
Sbjct: 1 MLSKILYADSGTSQTQEMLKAMMDFPAVQKASITILHVVPPQITTE-----------AFT 49
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ E L + L + A KV ++ G P + +A + D+
Sbjct: 50 EKWAEGGKILADLLEDVAIE------------PSKVSTVLRQGDPKGVVCDVANEIDADL 97
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
IIMGS G L+ IL SV++ V + +N P+L+VK
Sbjct: 98 IIMGSRGLKRLEAILENSVSQYVFQLTNHPMLLVK 132
Score = 57.2 bits (136), Expect = 7e-08
Identities = 28/165 (16%), Positives = 64/165 (37%), Gaps = 31/165 (18%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLK-AEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
K+++ D S AE AL+ E+IL V + + +
Sbjct: 141 KRVMVALDKSAAAEYALELALELLRDYPEGELILARVNPDLKPDLLPL------------ 188
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDII 124
++ E + + + +G + + G P E++ ++AED D++
Sbjct: 189 ----------SRQEIEENPVLAPAIAKAKRLGIAYRCTVTGGKPGEKLCELAEDYNADLM 238
Query: 125 IMGSHGK--------TNLKEILLGSVTENVIKKSNKPVLVVKRKN 161
++GS + +L +L S+++ + + PVL+ +++
Sbjct: 239 LLGSPDRRPSIAKSLPDLDRLLGTSLSDYIRVNAPCPVLLTRKEG 283
dbj|BAA16954| (D90902) hypothetical protein [Synechocystis sp.]
Length = 291
Score = 109 bits (269), Expect = 2e-23
Identities = 35/156 (22%), Positives = 69/156 (43%), Gaps = 21/156 (13%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M+K L TDFS+ + V+ ++I LH + E
Sbjct: 1 MFKHCLICTDFSDGLQRLAGFVEELSLSGITKLIFLHTVSVWE----------------- 43
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+E ++ +EAK +E++ ++ G +VK + + + ++ E E +D+
Sbjct: 44 ---DEHIADVDESKLKEAKTYLESLVGQVPP-GIEVKVEVSSVRYVDLVNQLVEQEAIDL 99
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
II G ++NL+ L GS T ++ K + PV++++
Sbjct: 100 IINGMPVRSNLESKLFGSHTLSLAKSTKVPVMILRP 135
Score = 43.6 bits (101), Expect = 9e-04
Identities = 29/158 (18%), Positives = 63/158 (39%), Gaps = 19/158 (12%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
+++ +L P D S ++ +K+ K + L + G+
Sbjct: 153 LWRNLLVPYDASSAGNYLIERLKSALEKA------------PPGKVESCYFLSILEDGMR 200
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ EL +EA+ K+ IK++ + + + G P +EI+ A +
Sbjct: 201 RP------ELLEIRRQEAEAKLAEIKQQFSPLVPNIITEVRHGSPVQEILDTAFVNDITA 254
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKN 161
I + S + L + + S+T++++ +S P+L K
Sbjct: 255 IAVASR-RATLLDWTVPSLTDSILNRSWFPLLFFSPKG 291
emb|CAA74460.1| (Y14080) hypothetical protein [Bacillus subtilis]
>gi|2633304|emb|CAB12808| (Z99109) similar to
hypothetical proteins [Bacillus subtilis]
Length = 184
Score = 106 bits (263), Expect = 9e-23
Identities = 35/166 (21%), Positives = 66/166 (39%), Gaps = 11/166 (6%)
Query: 4 MY--KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVID-----EREIKKRDIFSLL 56
M+ +I+ D SE ++ AL + + H D R
Sbjct: 19 MFHADRIIVAFDGSENSKKALLTAIDLAKTVNAAITVAHSHDMKDNQTVIDPPRPAAEAS 78
Query: 57 LGVAGLNKSVEEFENELKN----KLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEI 112
G+ + +++ + + + + + L + I+ G P E I
Sbjct: 79 YISGGMTSVPDPLISDVTSPEPMIYEDRTEEVIAEARMMLNEQQADGDIDILEGDPAESI 138
Query: 113 VKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
++ A D+I+ GS + LK+++ GSV+E + KS+ PVL+VK
Sbjct: 139 IEHANRISADMIVTGSRDQNRLKKLIFGSVSEKLSAKSDIPVLIVK 184
dbj|BAA30650| (AP000006) 208aa long hypothetical protein [Pyrococcus horikoshii]
Length = 208
Score = 105 bits (260), Expect = 2e-22
Identities = 43/160 (26%), Positives = 72/160 (44%), Gaps = 37/160 (23%)
Query: 2 SVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAG 61
S ++++ L D SE +E +K+++ +K E IL HV+D ++
Sbjct: 79 SNIFERPLVALDLSECSEKIIKNIRNLPEVK--EAILFHVVDYGKV-------------- 122
Query: 62 LNKSVEEFENELKNKLTEEAKNKMENIKKELEDVG----FKVKDIIVVGIPHEEIVKIAE 117
EE + + N KK L + G +K+K I GI I+ A
Sbjct: 123 -----------------EELEANINNAKKALSEYGKLLPWKIKVEIQAGIASRGIIGAAI 165
Query: 118 DEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
+ ++++G GK+ LKE+LLGS E VI+ P L++
Sbjct: 166 NNVATLVVIGKKGKSILKELLLGSTAERVIRDCRLPTLLI 205
Score = 82.1 bits (200), Expect = 2e-15
Identities = 23/63 (36%), Positives = 45/63 (70%)
Query: 96 GFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVL 155
G V+ ++ +GIP EI ++A++E V++I++ S G+ L+++LLGS N+ + + KPVL
Sbjct: 2 GINVETVVRIGIPSLEISEVAKEENVNLIVIPSKGQNILRQMLLGSTASNLARITRKPVL 61
Query: 156 VVK 158
+++
Sbjct: 62 ILR 64
gi|2648610 (AE000970) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 283
Score = 102 bits (252), Expect = 2e-21
Identities = 45/155 (29%), Positives = 75/155 (48%), Gaps = 18/155 (11%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M KK+L+P DFS +E A + + F+ L+ L
Sbjct: 1 MIKKVLFPVDFSVVSEYAFGNCIPKFFSTGA------------LTSSFSFTRLMLTCNLL 48
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+S++ L+ L + EN + ++ G K + ++ +G P EI KIAE+E VD+
Sbjct: 49 RSLK-----LQKSLKKVHGYDREN-CRRVQRDGDKRERVVRLGTPALEIAKIAEEENVDL 102
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
I M G+ ++E+L+GS NV + + KPVL+V+
Sbjct: 103 IYMPMKGENIIREMLIGSTAANVARVAKKPVLLVR 137
Score = 86.8 bits (212), Expect = 8e-17
Identities = 38/153 (24%), Positives = 71/153 (45%), Gaps = 29/153 (18%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
+ + L+ DFS+ E ++ + FK +E ILLHV+D +
Sbjct: 157 FDRPLFALDFSKCTEKIIQTTELFK-ELVKEAILLHVVDYGK------------------ 197
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDII 124
++++ E + + +K+ E + F + ++ G +EI+ A G +I
Sbjct: 198 ---------ESEVEENIQKATQKLKEIAEKLDFPSEVVVHSGDASKEILMTAPSVGATLI 248
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++G G+ L ++L+GS E VI+ S PVL+V
Sbjct: 249 VIGKRGRNIL-QLLMGSTAEIVIRNSVLPVLIV 280
gi|1787640 (AE000234) putative filament protein [Escherichia coli]
Length = 168
Score = 98.9 bits (243), Expect = 2e-20
Identities = 39/158 (24%), Positives = 69/158 (42%), Gaps = 15/158 (9%)
Query: 3 VMYKKILYPTDFS--ETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
M + IL P D S E + + HV+ + EV L VI + +
Sbjct: 24 FMNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELP 83
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG 120
+ + L EAK+++E I K+ + +V + G P + I+++A+
Sbjct: 84 AM------------DDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILELAKKIP 131
Query: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+II+ SH + ++ LLGS V++ + VLVV+
Sbjct: 132 AHMIIIASH-RPDITTYLLGSNAAAVVRHAECSVLVVR 168
sp|P37903|UP03_ECOLI UNKNOWN PROTEIN 2D_000B3L FROM 2D-PAGE >gi|1742248|dbj|BAA14980|
(D90775) Unknown protein from 2D-PAGE (SPOT 2D_000B3L)
(fragment). [Escherichia coli]
Length = 144
Score = 98.1 bits (241), Expect = 3e-20
Identities = 39/157 (24%), Positives = 69/157 (43%), Gaps = 15/157 (9%)
Query: 4 MYKKILYPTDFS--ETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAG 61
M + IL P D S E + + HV+ + EV L VI + +
Sbjct: 1 MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPA 60
Query: 62 LNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV 121
+ + L EAK+++E I K+ + +V + G P + I+++A+
Sbjct: 61 M------------DDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILELAKKIPA 108
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+II+ SH + ++ LLGS V++ + VLVV+
Sbjct: 109 HMIIIASH-RPDITTYLLGSNAAAVVRHAECSVLVVR 144
emb|CAB08889| (Z95554) hypothetical protein Rv1636 [Mycobacterium tuberculosis]
Length = 146
Score = 96.6 bits (237), Expect = 1e-19
Identities = 37/158 (23%), Positives = 71/158 (44%), Gaps = 19/158 (12%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIK-KRDIF-SLLLGVAGL 62
YK ++ TD S+++ A+ A+ +I + + E DI V G
Sbjct: 4 YKTVVVGTDGSDSSMRAVDRAAQIAGADAKLIIASAYLPQHEDARAADILKDESYKVTG- 62
Query: 63 NKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFK-VKDIIVVGIPHEEIVKIAEDEGV 121
+ E + + K+ + G K V++ +VG P + +V +A++E
Sbjct: 63 TAPIYE---------------ILHDAKERAHNAGAKNVEERPIVGAPVDALVNLADEEKA 107
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
D++++G+ G + + LLGSV NV +++ VL+V
Sbjct: 108 DLLVVGNVGLSTIAGRLLGSVPANVSRRAKVDVLIVHT 145
emb|CAA22850.1| (AL035248) hypothetical protein [Schizosaccharomyces pombe]
Length = 601
Score = 93.8 bits (230), Expect = 6e-19
Identities = 36/158 (22%), Positives = 67/158 (41%), Gaps = 28/158 (17%)
Query: 10 YPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVEEF 69
D S + A + + +I++ VI+ + R
Sbjct: 435 LTLDLSSESLHAAEWAVGILLRNGDTLIIVDVIECDDPSAR------------------- 475
Query: 70 ENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIP--HEEIVKIAEDEGVD----- 122
+K+++ E +E I K + + K + V I H E K E +D
Sbjct: 476 --AVKDRMESEQLETLEKITKYILKLLSKTVLEVEVNIEVIHHEKAKHLIIEMIDYIEPS 533
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
+++MGS G+++LK +LLGS + ++ KS+ PV+V ++K
Sbjct: 534 LVVMGSRGRSHLKGVLLGSFSNYLVNKSSVPVMVARKK 571
sp|P73475|YC30_SYNY3 HYPOTHETICAL 31.2 KD PROTEIN SLR1230 >gi|1652594|dbj|BAA17515|
(D90906) hypothetical protein [Synechocystis sp.]
Length = 287
Score = 91.5 bits (224), Expect = 3e-18
Identities = 35/152 (23%), Positives = 67/152 (44%), Gaps = 31/152 (20%)
Query: 7 KILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSV 66
K+L+ D S + + L+ + LH++
Sbjct: 167 KVLFAYDGSASCQKILQF---LAGSSLLADLPLHIVTVG--------------------- 202
Query: 67 EEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIM 126
K +A + +K LE GFK++ ++VG E IV+ ED +D+++M
Sbjct: 203 -------KTNQDPQAIANLGTAEKVLEKAGFKLEVELLVGHAEEAIVRYQEDNAIDLLLM 255
Query: 127 GSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
G+HG + ++ +++GS T V++K++ PVL +
Sbjct: 256 GAHGHSRIRHLVIGSTTAQVLRKTSIPVLTFR 287
Score = 56.8 bits (135), Expect = 9e-08
Identities = 36/159 (22%), Positives = 65/159 (40%), Gaps = 11/159 (6%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLG-----VA 60
K IL TD S+ A+ + + + + +L+V D R K + +L
Sbjct: 2 KNILLCTDGSDFAQQSYPYAAWLASKLGGNIKVLYVTDIRAQKAVESVNLSGSIGLGTSE 61
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDE- 119
L K + + E+ ++AK + K L+ G + ++ ++ ED
Sbjct: 62 ELLKQLVDLEHTKAKLNHQKAKLVLATAKNTLQQAGIESVQVM---HKTGFLLDCLEDLK 118
Query: 120 -GVDIIIMGSHGK-TNLKEILLGSVTENVIKKSNKPVLV 156
D+II+G G+ + LG+ E +I+ KP LV
Sbjct: 119 GDFDVIILGKRGETAKFAQGHLGANMERIIRSIPKPCLV 157
sp|Q10851|YK05_MYCTU HYPOTHETICAL 30.9 KD PROTEIN RV2005C >gi|1403448|emb|CAA98383|
(Z74025) hypothetical protein Rv2005c [Mycobacterium
tuberculosis]
Length = 295
Score = 89.2 bits (218), Expect = 2e-17
Identities = 34/152 (22%), Positives = 69/152 (45%), Gaps = 19/152 (12%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
+L D S +E+A + + E+I +H + E+ + + GL+ S
Sbjct: 162 VLVGIDGSPVSELATAVAFDEASRRGVELIAVHAWSDVEV---------VELPGLDFSAV 212
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
+ E EL ++ ++ D V ++V P ++V+ + ++++G
Sbjct: 213 QQEAELS------LAERLAGWQERYPD--VPVSRVVVCDRPARKLVQ--KSASAQLVVVG 262
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
SHG+ L +LLGSV+ V+ + PV+V ++
Sbjct: 263 SHGRGGLTGMLLGSVSNAVLHAARVPVIVARQ 294
Score = 68.9 bits (166), Expect = 2e-11
Identities = 27/155 (17%), Positives = 65/155 (41%), Gaps = 22/155 (14%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
++ D S ++ A ++ + ++HV++ ++ V
Sbjct: 10 VVVGVDGSLESDAAACWGATDAAMRNIPLTVVHVVN-ADVATWPPMPY-----PETWGVW 63
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDV-----GFKVKDIIVVGIPHEEIVKIAEDEGVD 122
+ +E + + N K ++ VK +V P +V+I+ + +
Sbjct: 64 Q---------EDEGRQIVANAVKLAKEAVGADRKLSVKSELVFSTPVPTMVEISNE--AE 112
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++++GS G+ L LLGSV+ ++++++ PV V+
Sbjct: 113 MVVLGSSGRGALARGLLGSVSSSLVRRAGCPVAVI 147
gb|AAD47991.1| (AF157801) hypothetical protein B [Pseudomonas sp. R9]
Length = 283
Score = 88.0 bits (215), Expect = 4e-17
Identities = 22/103 (21%), Positives = 56/103 (54%), Gaps = 1/103 (0%)
Query: 56 LLGVAGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKI 115
+L + L K + + + +EA +++ +K L + GF V+ G +
Sbjct: 182 MLAASPLLKGLPIHL-VMVGPVNDEASAQLDWAQKVLINAGFTVRAETRSGEIERTLHAY 240
Query: 116 AEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
++ G+D+++MG +G + +++ L+GS T ++++ + P+L+++
Sbjct: 241 QKEHGIDLLVMGDYGHSRIRQFLVGSTTTSMLRTTTSPLLLLR 283
Score = 52.2 bits (123), Expect = 2e-06
Identities = 30/156 (19%), Positives = 68/156 (43%), Gaps = 9/156 (5%)
Query: 7 KILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSV 66
++ D S +A + + LHV+D+R+ S +G+ +
Sbjct: 3 HVIACIDGSTSAPAVCDYAAWASLSLEAPLTFLHVLDQRQYPVAADLSGNIGLGSREHLL 62
Query: 67 EEF--ENELKNKL-TEEAKNKMENIKKELEDVGFKV-KDIIVVGIPHEEIVKIAEDEGVD 122
+E +E + KL E+ + + K+ + G + + G E + ++ +
Sbjct: 63 DELASLDEQRGKLALEQGRIMLAAAKERAVNDGVRAPESKQRHGDLLESLQELQSETR-- 120
Query: 123 IIIMGSHGKT--NLKEILLGSVTENVIKKSNKPVLV 156
++++G G++ L + +GS E+VI+ ++P+LV
Sbjct: 121 LLVIGRQGESSGGLSQH-VGSQLESVIRIMHRPILV 155
gi|2650517 (AE001097) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 167
Score = 87.2 bits (213), Expect = 6e-17
Identities = 27/97 (27%), Positives = 53/97 (53%), Gaps = 3/97 (3%)
Query: 65 SVEEFENELK--NKLTEEAKNKMENIKKELEDVGFKVKDI-IVVGIPHEEIVKIAEDEGV 121
+ +E E+K L +A +E ++ LE+ G +VK + I++G EE++++ +
Sbjct: 50 PIASYEKEMKIYTSLRVKASKFVEFYRERLEEAGLEVKQVKIILGNVSEEVLRLEKLLNP 109
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
D+I+ G + LK +L G + +I ++ PV+V K
Sbjct: 110 DLIVFGMEKRGFLKRLLRGDPYKEIIYETKAPVMVCK 146
gb|AAF16649.1|AC011661_27 (AC011661) T23J18.3 [Arabidopsis thaliana]
Length = 875
Score = 86.0 bits (210), Expect = 1e-16
Identities = 36/157 (22%), Positives = 64/157 (39%), Gaps = 5/157 (3%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
+KI D S+ + A++ + V+LLHV + D + + + +
Sbjct: 671 RKIGIAVDLSDESAYAVQWAVQNYLRSGDAVVLLHVQPTSVLYGAD-WGAMDLSPQWDPN 729
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIV-VGIPHEEIVKIAEDEGVDII 124
EE + +L++ K ++ + L + K IV E + E G+ +
Sbjct: 730 NEESQRKLEDDFDIVTNKKASDVAQPLVEADIPFKIHIVKDHDMKERLCLEVERLGLSTL 789
Query: 125 IMGSHGKTNLKE---ILLGSVTENVIKKSNKPVLVVK 158
IMGS G K LGSV++ + PV+VV+
Sbjct: 790 IMGSRGFGATKRSSKGRLGSVSDYSVHHCACPVVVVR 826
dbj|BAA79051.1| (AP000058) 243aa long hypothetical protein [Aeropyrum pernix]
Length = 243
Score = 86.0 bits (210), Expect = 1e-16
Identities = 30/89 (33%), Positives = 47/89 (52%), Gaps = 2/89 (2%)
Query: 73 LKNKLTEEAKNKMENIKKELEDVGFKVK--DIIVVGIPHEEIVKIAEDEGVDIIIMGSHG 130
L + + A+ K++ IKK + + G V + I VG P I ++AE+ G I+MGS G
Sbjct: 14 LIRSIEKNAREKLDKIKKLMMEKGANVVVYEDIPVGNPGTVISEVAEEVGATEIVMGSKG 73
Query: 131 KTNLKEILLGSVTENVIKKSNKPVLVVKR 159
+ + LGS +K S KPV+ +K
Sbjct: 74 LGIFRILPLGSTVRETVKISRKPVIRLKT 102
Score = 57.2 bits (136), Expect = 7e-08
Identities = 29/153 (18%), Positives = 62/153 (39%), Gaps = 29/153 (18%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
+++KIL D + T++ + + + +VIL H+I+ +
Sbjct: 117 LFRKILVGADRN-TSKSMIDYAVNAASTDDGKVILAHIIEPPLEEPSY------------ 163
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
E KN + +K E+ G +V+ ++ G P + + IA
Sbjct: 164 ----------------EVKNVFKYGEKAGEERGVEVEIVVARGRPDKMLTAIASQMDASS 207
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLV 156
+++G + L E++LGS + ++ P++V
Sbjct: 208 VLVGRTVERRLSELILGSTLDRLMTLCELPLIV 240
gb|AAF01537.1|AC009325_7 (AC009325) unknown protein [Arabidopsis thaliana]
Length = 296
Score = 85.7 bits (209), Expect = 2e-16
Identities = 27/144 (18%), Positives = 59/144 (40%), Gaps = 11/144 (7%)
Query: 19 EIALKHVKAFKTLKAEE---VILLHVIDEREIKKRDIFSLLLGVAGLNKSVEEFENELKN 75
+ A + ++LLHV E V + S E+F +++
Sbjct: 149 KRAFEWTLEKIVRSNTSDFKILLLHVQVVDEDG-------FDDVDSIYASPEDFR-DMRQ 200
Query: 76 KLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLK 135
+ + +E + ++G + I G P + I + + D +++GS G +
Sbjct: 201 SNKAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEVKRVRPDFLVVGSRGLGRFQ 260
Query: 136 EILLGSVTENVIKKSNKPVLVVKR 159
++ +G+V+ +K + PV+ +KR
Sbjct: 261 KVFVGTVSAFCVKHAECPVMTIKR 284
emb|CAB53139.1| (AL109962) hypothetical protein SCJ1.21 [Streptomyces coelicolor
A3(2)]
Length = 152
Score = 84.1 bits (205), Expect = 6e-16
Identities = 31/153 (20%), Positives = 66/153 (42%), Gaps = 17/153 (11%)
Query: 7 KILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSV 66
+++ D S ++ AL+ + V +HV D +G AG
Sbjct: 9 RVVVGVDGSPSSYAALRWADRYARAVGGVVEAVHVWDTP---------SAVGFAGPAIDP 59
Query: 67 EEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIM 126
+ + + + E +E G K +I+V G P E +++ ++ G +++++
Sbjct: 60 DFDLEQARERFAAE----LEATFPGERPPGLK--EILVEGDPSETLIRASQ--GAELLVV 111
Query: 127 GSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
G G+ +LGSV++ + + PV+VV++
Sbjct: 112 GRRGRGAFARAMLGSVSQRCAQHAACPVVVVRQ 144
sp|P87132|YDM1_SCHPO HYPOTHETICAL PROTEIN C57A7.01 IN CHROMOSOME I
>gi|2104436|emb|CAB08759.1| (Z95396) hypothetical
protein [Schizosaccharomyces pombe]
Length = 131
Score = 82.9 bits (202), Expect = 1e-15
Identities = 29/95 (30%), Positives = 52/95 (54%), Gaps = 7/95 (7%)
Query: 73 LKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIP--HEEIVKIAEDEGVD-----III 125
+K+++ E +E I K + + K + V I H E K E +D +++
Sbjct: 7 VKDRMESEQLETLEKITKYILKLLSKTVLEVEVNIEVIHHEKAKHLIIEMIDYIEPSLVV 66
Query: 126 MGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
MGS G+++LK +LLGS + ++ KS+ PV+V ++K
Sbjct: 67 MGSRGRSHLKGVLLGSFSNYLVNKSSVPVMVARKK 101
gb|AAF11911.1|AE002067_3 (AE002067) conserved hypothetical protein [Deinococcus radiodurans]
Length = 160
Score = 82.1 bits (200), Expect = 2e-15
Identities = 36/153 (23%), Positives = 69/153 (44%), Gaps = 16/153 (10%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
++++L DFS ++ AL+ + + L HV D R + D+ GV +
Sbjct: 18 FQRLLVGIDFSPSSLHALEVART--RFPGARLRLAHVTDARAVAAPDVVG---GVTPIMP 72
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDII 124
L L + N++ + ++ E+ ++VG P ++ A G D+I
Sbjct: 73 DPG-----LLQTLEDADSNRLSGLIRDGEES------ELLVGDPITGLLDAARAWGADLI 121
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++G+H + L+ +GS E ++ +S PVL V
Sbjct: 122 VVGTHPQGALEHFFIGSSAEKLVGRSAVPVLCV 154
emb|CAA19726.1| (AL030978) putative protein [Arabidopsis thaliana]
Length = 259
Score = 80.2 bits (195), Expect = 8e-15
Identities = 35/160 (21%), Positives = 58/160 (35%), Gaps = 7/160 (4%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREI--KKRDIFSLLLGVAGLN 63
+KI D SE + A++ + V++LHV + L
Sbjct: 45 RKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSVLFGADWGPLPLQTPPPPSA 104
Query: 64 K-SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIV-VGIPHEEIVKIAEDEGV 121
+ +K+ ++ K L++ GF K IV E + E +
Sbjct: 105 ATDPGAQPKPSQEDFDAFTSSKVADLAKPLKEAGFPHKIHIVKDHDMRERLCLETERLNL 164
Query: 122 DIIIMGSHGKTNLKE---ILLGSVTENVIKKSNKPVLVVK 158
+IMGS G K LGSV++ + PV+VV+
Sbjct: 165 SAVIMGSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVR 204
emb|CAA17240| (AL021899) hypothetical protein Rv2026c [Mycobacterium
tuberculosis]
Length = 294
Score = 80.2 bits (195), Expect = 8e-15
Identities = 30/152 (19%), Positives = 58/152 (37%), Gaps = 19/152 (12%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
+L D S +E A + + +++ LH + L
Sbjct: 161 VLVGIDGSPASEAATALAFDEASRRRVDLVALHA--------WTDLGMF---PVLGMDWR 209
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
E E E ++ +++ D +V +V P +++ +E ++++G
Sbjct: 210 EREKR----EAEVLAERLAGWQEQYPD--VRVHRSLVCDKPARWLLEHSEQ--AQLVVVG 261
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
SHG+ +LLGSV+ V PV+VV+
Sbjct: 262 SHGRGGFSGMLLGSVSSAVAHSVRIPVIVVRP 293
Score = 79.0 bits (192), Expect = 2e-14
Identities = 28/154 (18%), Positives = 64/154 (41%), Gaps = 12/154 (7%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
IL D S + A+ ++ + LLH++ + V L ++
Sbjct: 10 ILVGVDGSAQSNAAVAWAAREAVMRQLPITLLHIVAPVVV--------GWPVGQLYANMT 61
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
E++ + ++ E+A+ + N E +V +V ++ ++ ++++G
Sbjct: 62 EWQKDNAQQVIEQAREALTN--SLGESKPPQVHTELVFSNVVPTLIDASQQ--AWLMVVG 117
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKN 161
S G L +LLGS++ ++ + PV ++ N
Sbjct: 118 SQGMGALGRLLLGSISTALLHHARCPVAIIHSGN 151
emb|CAB53424.1| (AL109989) hypothetical protein SCJ12.12c [Streptomyces coelicolor
A3(2)]
Length = 301
Score = 79.4 bits (193), Expect = 1e-14
Identities = 34/155 (21%), Positives = 59/155 (37%), Gaps = 17/155 (10%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M + I D S + A + TL+ V LLHV + L
Sbjct: 1 MARTITVGLDGSPESRAAAEWAAREGTLRRVPVRLLHVWQPVP-------EPMAQAPLLG 53
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ E + T E L G +V G P + ++ A ++
Sbjct: 54 AETHQHWTERIPRDTAEGL--------RLRHPGVEVTTEQATGNPADALL--AGTLDAEL 103
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+++GS + L L+GSV ++VI ++ PV++V+
Sbjct: 104 LVLGSRALSGLTGFLVGSVGQSVIARTETPVILVR 138
Score = 49.0 bits (115), Expect = 2e-05
Identities = 22/153 (14%), Positives = 52/153 (33%), Gaps = 15/153 (9%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
++ ++ D E L + + + + +S+ G
Sbjct: 161 FRPVVVGLDTGSPDEAVLSFAFEEARRRRAPLTA---VRAWNLPSSYTYSIAAGFDP--- 214
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDII 124
EL E + +++ D +V + +G P E ++ A ++
Sbjct: 215 -----REELARAQAEALGEALLPWREKYPD--VEVTETCRLGSPAEHLIDAAR--DASLV 265
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++G + + + +G+V V+ + PV VV
Sbjct: 266 VVGRRIRRSPFGVHIGAVAHAVMHHATTPVAVV 298
gb|AAC63627.1| (AC005309) unknown protein [Arabidopsis thaliana]
Length = 162
Score = 78.3 bits (190), Expect = 3e-14
Identities = 29/159 (18%), Positives = 60/159 (37%), Gaps = 16/159 (10%)
Query: 8 ILYPTDFSETAEIALKHVKA-----FKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGL 62
++ D SE + AL+ + ++ ++H L G
Sbjct: 10 MVVGVDDSEQSTYALEWTLDRFFAPYAPNYPFKLFIVHAKPNAVSAVG------LAGPGT 63
Query: 63 NKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVV-GIPHEEIVKIAEDEGV 121
+ V + +LK+ A +E K + I V G + ++ +
Sbjct: 64 AEVVPYVDADLKHT----AAKVVEKAKAICQSRSVHGAVIEVFEGDARNILCEVVDKHHA 119
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
I+++GSHG +K +LGS ++ ++ V++VK+
Sbjct: 120 SILVVGSHGYGAIKRAVLGSTSDYCAHHAHCSVMIVKKP 158
emb|CAB68183.1| (AL137082) putative protein [Arabidopsis thaliana]
Length = 174
Score = 77.1 bits (187), Expect = 7e-14
Identities = 26/140 (18%), Positives = 49/140 (34%), Gaps = 14/140 (10%)
Query: 7 KILYPTDFSETAEIALKHVKAFKTL----------KAEEVILLHVIDEREIKKRDIFSLL 56
K++ D S+ + AL+ + + + LLHV
Sbjct: 31 KVMVAIDESKNSFDALEWAVDHLRVVISAEPETGQEGGLLTLLHVHPTYLQYIYPSGGTA 90
Query: 57 LGVAGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIA 116
V + E + T +E + ++ K + +I+ G P E I +
Sbjct: 91 SAVYATDSVPEPMRKAREESTTNLFTRALEICRGKM----VKTETMILEGDPKEMICQAV 146
Query: 117 EDEGVDIIIMGSHGKTNLKE 136
E VD++++GS G +K
Sbjct: 147 EQTHVDLLVVGSRGLGMIKR 166
emb|CAB53148.1| (AL109962) hypothetical protein SCJ1.30c [Streptomyces coelicolor
A3(2)]
Length = 328
Score = 77.1 bits (187), Expect = 7e-14
Identities = 35/155 (22%), Positives = 58/155 (36%), Gaps = 17/155 (10%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M + I D S + A + L+ V LLHV + +
Sbjct: 29 MTRTITVGIDGSPESHAAAEWAAREAELRDLPVRLLHVWEPA--------------PAVL 74
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ TE K+ L G V G P + +V+ A+ G ++
Sbjct: 75 AQDSILGAKTHQHWTERVPQKVGEG-LRLRHPGVVVTSDQRSGPPADTLVRDAD--GAEL 131
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+++GS + L L GSV ++VI S PV++V+
Sbjct: 132 LVLGSRAPSGLGGFLAGSVGQSVIAHSETPVVLVR 166
Score = 38.9 bits (89), Expect = 0.022
Identities = 11/61 (18%), Positives = 30/61 (49%), Gaps = 2/61 (3%)
Query: 97 FKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLV 156
+V + G P + + + + + ++++G + + + +G+V V+ + + PV V
Sbjct: 267 VEVVEASRPGSPADLLAEASHE--ASLVVVGRRIRPSPLGVHIGAVAHAVLHRVSAPVAV 324
Query: 157 V 157
V
Sbjct: 325 V 325
emb|CAA04212| (AJ000662) hypothetical protein [Wolinella succinogenes]
Length = 138
Score = 75.5 bits (183), Expect = 2e-13
Identities = 40/154 (25%), Positives = 71/154 (45%), Gaps = 18/154 (11%)
Query: 6 KKILYPTDFSETAEIALKHVKA-FKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
KK+L+ D +E E A +++ F + L+HV E +L G A L
Sbjct: 2 KKLLFAIDDTEACERAAQYILDMFGKDADCTLTLIHVKPE---------FMLYGEAVLAA 52
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDII 124
+E++ K E+AK + + G +I G P E +++ A+ +++
Sbjct: 53 Y-----DEIEMKEEEKAKLLTQKFSTFFTEKGINPFVVIKEGEPVEMVLEEAK--DYNLL 105
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
I+GS + L +I S ++ I+K+ PVL+VK
Sbjct: 106 IIGSSENSFLNKIF-ASHQDDFIQKAPIPVLIVK 138
emb|CAB53134.1| (AL109962) conserved hypothetical protein SCJ1.16c [Streptomyces
coelicolor A3(2)]
Length = 294
Score = 74.7 bits (181), Expect = 4e-13
Identities = 34/165 (20%), Positives = 64/165 (38%), Gaps = 22/165 (13%)
Query: 1 MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
M +M ++ D S+ + +A+ + + L++ + + L
Sbjct: 1 MGMMALPLVVGVDGSDGSLLAIDWAVDEAQRQGLPLRLVYA------SLWERYEGALPAM 54
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELE--DVGFKVKDIIVVGIPHEEIVKI-AE 117
G + E+ E + + + D G V V P E + + AE
Sbjct: 55 GRERPSEQVMAEN----------IVGTAAERVRRYDPGLTVDTDTV---PAEAVSALLAE 101
Query: 118 DEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
++ GS G+ LK LLGSV+ V +++ PV+VV+ + S
Sbjct: 102 GRHATAVVTGSRGRGELKGALLGSVSLAVASRADCPVVVVRGEKS 146
Score = 47.9 bits (112), Expect = 4e-05
Identities = 15/82 (18%), Positives = 38/82 (46%), Gaps = 4/82 (4%)
Query: 78 TEEAKNKMENIKKEL--EDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLK 135
A ++ + E E +++ + G P +++ + D++++G+ ++
Sbjct: 211 ERRASALIDTLVAEAAAEHPSVRLRKTTIEG-PARKVL-VHRTAAADLVVVGARHRSGHF 268
Query: 136 EILLGSVTENVIKKSNKPVLVV 157
+ LG VT +++ + PV VV
Sbjct: 269 GLQLGRVTHTLLQHAACPVAVV 290
emb|CAB08619| (Z95387) hypothetical protein Rv2623 [Mycobacterium tuberculosis]
Length = 297
Score = 74.4 bits (180), Expect = 5e-13
Identities = 24/151 (15%), Positives = 65/151 (42%), Gaps = 19/151 (12%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
+L D S +E+A + + +++ LH + ++ + G++
Sbjct: 162 VLVGVDGSSASELATAIAFDEASRRNVDLVALHAWSDVDVSEW---------PGIDWPA- 211
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
++ + ++ ++ + + ++V P ++V+ +E+ ++++G
Sbjct: 212 -----TQSMAEQVLAERLAGWQERYPN--VAITRVVVRDQPARQLVQRSEE--AQLVVVG 262
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
S G+ +L+GSV E V + + PV+V +
Sbjct: 263 SRGRGGYAGMLVGSVGETVAQLARTPVIVAR 293
Score = 61.1 bits (146), Expect = 5e-09
Identities = 26/155 (16%), Positives = 65/155 (41%), Gaps = 12/155 (7%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
I+ D S A++A++ L+ + L+H + E+ L G+ + +
Sbjct: 10 IIVGIDDSPAAQVAVRWAARDAELRKIPLTLVHAVSP-EVATWLEVPLP---PGVLRWQQ 65
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
+ L + +A +E + + +P +V +++ ++++G
Sbjct: 66 DHGRHLID----DALKVVEQASLRAGPPTVHSEIVPAAAVP--TLVDMSK--DAVLMVVG 117
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
G LLGSV+ +++ ++ PV+++ ++S
Sbjct: 118 CLGSGRWPGRLLGSVSSGLLRHAHCPVVIIHDEDS 152
emb|CAB57770.1| (Y18930) hypothetical protein [Sulfolobus solfataricus]
Length = 136
Score = 74.0 bits (179), Expect = 6e-13
Identities = 31/157 (19%), Positives = 63/157 (39%), Gaps = 29/157 (18%)
Query: 2 SVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAG 61
S +KIL P D SE + AL F ++ ++HV +
Sbjct: 9 SFWLRKILVPVDGSENSLRALDLAVDFGMRYGSKITIIHVCSD----------------- 51
Query: 62 LNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGV 121
++ + ++ ++ ++ + + ++ VK I EI+K+ +E
Sbjct: 52 -CNNMNDIQSLIEKRINNKIEYDLKI-----------VKINIKESSVSNEILKVINEEPY 99
Query: 122 DIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
D IIMG+ G + +I +GS + + V++V+
Sbjct: 100 DAIIMGARGTSLNSDINIGSTALAISINAPVSVILVR 136
emb|CAA73748| (Y13308) hypothetical protein [Yersinia enterocolitica]
Length = 288
Score = 74.0 bits (179), Expect = 6e-13
Identities = 28/132 (21%), Positives = 59/132 (44%), Gaps = 16/132 (12%)
Query: 39 LHVIDEREIKKRDIFSLLLGVAGLNKSVE-----------EFENELKNKLTEEAKNKMEN 87
L V+ E G +++E E + N EE
Sbjct: 148 LLVVPENYSAPSRAMFAYDGSEESRRNLERLTMSPLLRGLECHLVMVNGKKEELLA---- 203
Query: 88 IKKELEDVGFKVKDIIVVG-IPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENV 146
++ L D G + + G + +++ AE+ VD+I+MG++G + L++ +GS T +
Sbjct: 204 AQQILRDAGIENSTTHLTGQSVGDALIRYAEENAVDLIVMGAYGHSRLRQFFIGSHTSEM 263
Query: 147 IKKSNKPVLVVK 158
++K+ +P+L+++
Sbjct: 264 LQKTQQPLLILR 275
Score = 63.4 bits (152), Expect = 9e-10
Identities = 36/158 (22%), Positives = 65/158 (40%), Gaps = 11/158 (6%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAG-- 61
M + D S + ++ ++ LLHVI++ + LG+
Sbjct: 1 MNNTVTACVDGSLSTRSVCEYAAWAARTLQSQLALLHVIEKDSTPVVSDLTGTLGLDSQQ 60
Query: 62 -LNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFK-VKDIIVVGIPHEEIVKIAEDE 119
L + E E + L + K +E+ + L+ G V + G P E + AE
Sbjct: 61 LLTDELVEIEGQRNRLLMAQGKAILESCSELLQKQGSPDVLLMQKHGTPDEVL---AELS 117
Query: 120 GVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
+ ++++G G + +GS E+VI+ KP+LVV
Sbjct: 118 DLRLMVLGRRG----SQHPVGSHLESVIRLQKKPLLVV 151
emb|CAB53147.1| (AL109962) hypothetical protein SCJ1.29c [Streptomyces coelicolor
A3(2)]
Length = 283
Score = 73.2 bits (177), Expect = 1e-12
Identities = 28/155 (18%), Positives = 54/155 (34%), Gaps = 16/155 (10%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MY ++ D SE + A+ L A + ++ D E + + G
Sbjct: 1 MYLPLVVGVDGSEPSLRAVDWAADEAALHAVPLWVVF-GDLWERYEGAALAREPGKPS-- 57
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ ++ V V ++ + +
Sbjct: 58 ----------TDMQADDILAAAAIRAGRRHPDLV-VTTETVPDEAEHALICAGRN--ASM 104
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
I+MGS G++ + + LLGSV+ V S+ PV+V++
Sbjct: 105 IVMGSRGRSGIADRLLGSVSRTVAAGSDCPVVVLR 139
Score = 44.0 bits (102), Expect = 7e-04
Identities = 19/83 (22%), Positives = 35/83 (41%), Gaps = 8/83 (9%)
Query: 75 NKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNL 134
+ E A ++E ++ V+ V G P ++ A E ++++G +
Sbjct: 205 RRYEERAARELEAALEDAPPD-VAVRRYTVEG-PARAVLPAASAE-AGLLVIGRRTQGR- 260
Query: 135 KEILLGSVTENVIKKSNKPVLVV 157
LG V V+ +S PV+VV
Sbjct: 261 ----LGRVAHAVLHRSACPVVVV 279
sp|Q10862|YJ96_MYCTU HYPOTHETICAL 33.9 KD PROTEIN RV1996 >gi|1403459|emb|CAA98390|
(Z74025) hypothetical protein Rv1996 [Mycobacterium
tuberculosis]
Length = 317
Score = 73.2 bits (177), Expect = 1e-12
Identities = 29/153 (18%), Positives = 65/153 (41%), Gaps = 14/153 (9%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
++ D S T+ +A + + + +++ LH L LN +
Sbjct: 172 VVVGIDGSPTSGLAAEIAFDEASRRGVDLVALHA--------WSDMGPLD-FPRLNWAPI 222
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
E+ N L+++ + ++ + D V ++V P ++++A+ ++++G
Sbjct: 223 EWRN-LEDEQEKMLARRLSGWQDRYPD--VVVHKVVVCDRPAPRLLELAQT--AQLVVVG 277
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
SHG+ + LGSV+ V+ PV+V +
Sbjct: 278 SHGRGGFPGMHLGSVSRAVVNSGQAPVIVARIP 310
Score = 59.6 bits (142), Expect = 1e-08
Identities = 26/153 (16%), Positives = 60/153 (38%), Gaps = 7/153 (4%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
I+ D S + A++ ++ + L V+ + ++ +
Sbjct: 10 IVVGVDGSPCSHTAVEWAARDAQMRN---VALRVVQVVPPVITAPEGWAFEYSRFQEAQK 66
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIP-HEEIVKIAEDEG--VDII 124
E + L +A +E K + + + G H +IV + V ++
Sbjct: 67 REIVE-HSYLVAQAHQIVEQAHKVALEASSSGRAAQITGEVLHGQIVPTLANISRQVAMV 125
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++G G+ + LLGSV+ ++++ ++ PV V+
Sbjct: 126 VLGYRGQGAVAGALLGSVSSSLVRHAHGPVAVI 158
sp|P72745|YB01_SYNY3 HYPOTHETICAL 12.2 KD PROTEIN SLR1101 >gi|1651833|dbj|BAA16760|
(D90900) hypothetical protein [Synechocystis sp.]
Length = 108
Score = 71.2 bits (172), Expect = 4e-12
Identities = 23/80 (28%), Positives = 43/80 (53%)
Query: 78 TEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEI 137
+E + +E +++ + + + ++G P + I + A+ + DII++G G+ L EI
Sbjct: 25 EQEGLDTLEKRRQQALALDIECQAEQILGSPGKIICQRAKQDNSDIIVVGHRGRWGLSEI 84
Query: 138 LLGSVTENVIKKSNKPVLVV 157
LLGSV V ++ V VV
Sbjct: 85 LLGSVGNYVFHHAHCCVFVV 104
gi|2649611 (AE001036) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 130
Score = 70.8 bits (171), Expect = 5e-12
Identities = 28/153 (18%), Positives = 61/153 (39%), Gaps = 27/153 (17%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
I+ D S+ + L+ E+++ +H L G +
Sbjct: 3 IVVAVDHSDRTPRVVDFAIEEAKLRGEKLLFVH--------------SLYGGDKTSAK-- 46
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVV--GIPHEEIVKIAEDEGVDIII 125
E + ++ + E+ G + + ++V P E+IV+ A++ +I+
Sbjct: 47 ---------EIEAGERLLDYVVSLAENRGVEAEKHLLVRGKEPEEDIVEFADEVEASMIV 97
Query: 126 MGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+G + ++L GSV + VI + +PV+ +K
Sbjct: 98 IGVRKRRPAGKLLFGSVAQQVILHAKQPVVCIK 130
emb|CAB08618| (Z95387) hypothetical protein Rv2624c [Mycobacterium tuberculosis]
Length = 272
Score = 68.5 bits (165), Expect = 3e-11
Identities = 31/157 (19%), Positives = 60/157 (37%), Gaps = 26/157 (16%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
K I+ D S A A +A + L+ VI S
Sbjct: 10 KTIIVGIDGSHAAITAALWGVDEAISRAVPLRLVSVIKPTH-----------------PS 52
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVG--FKVKDIIVVGIPHEEIVKIAEDEGVDI 123
++++ +L + A+ + + +E G K++ I G +V+ + ++
Sbjct: 53 PDDYDRDLAH-----AERSLREAQSAVEAAGKLVKIETDIPRGPAGPVLVEASR--DAEM 105
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRK 160
I +GS G +LGS + +K++ PV V++ K
Sbjct: 106 ICVGSVGIGRYASSILGSTATELAEKAHCPVAVMRSK 142
gb|AAF26906.1|AF210843_3 (AF210843) unknown [Sorangium cellulosum]
Length = 713
Score = 67.0 bits (161), Expect = 8e-11
Identities = 28/153 (18%), Positives = 56/153 (36%), Gaps = 19/153 (12%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
++IL P E + A E++LL
Sbjct: 568 RRILVPIIGLEYSFAAADLAAHVALAWDAELVLLSSAQTDP----------------GAV 611
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVG-IPHEEIVKIAEDEGVDII 124
V +++ A++ ++ +G +V + VG P +EI + D++
Sbjct: 612 VWRDREP--SRVRAVARSVVDEAVFRGRRLGVRVSSRVHVGAHPSDEITRELARAPYDLL 669
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++G + L + LGS E+V+ +S PV ++
Sbjct: 670 VLGCYDHGPLGRLYLGSTVESVVVRSRVPVALL 702
gi|2650286 (AE001080) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 84
Score = 66.6 bits (160), Expect = 1e-10
Identities = 27/75 (36%), Positives = 41/75 (54%), Gaps = 3/75 (4%)
Query: 86 ENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEI--LLGSVT 143
+ +K LE + K I+ G + I+ AE+EGVD+I+M G + EI LGS
Sbjct: 11 KEAEKALEKYDVE-KRIVKAGKCWKVIIDTAEEEGVDMIVMTERGSGAVAEIGDALGSCA 69
Query: 144 ENVIKKSNKPVLVVK 158
E V + + PVL+V+
Sbjct: 70 EKVARHARNPVLIVR 84
gi|2649775 (AE001047) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 134
Score = 66.6 bits (160), Expect = 1e-10
Identities = 34/161 (21%), Positives = 65/161 (40%), Gaps = 30/161 (18%)
Query: 1 MSVMYKKILYPTDF-SETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGV 59
M M I+ D S+ AE L+ L+ V ++H +
Sbjct: 1 MIYM--PIVVAVDKKSDRAERVLRFAAEEARLRGVPVYVVHSLPGGG------------- 45
Query: 60 AGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVV--GIPHEEIVKIAE 117
K++ EAK + + G + ++ ++V P ++IV A+
Sbjct: 46 ------------RTKDEDIIEAKETLSWAVSIIRKEGAEGEEHLLVRGKEPPDDIVDFAD 93
Query: 118 DEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+ I++G ++ +++ GSV +VI K+NKPV+ +K
Sbjct: 94 EVDAIAIVIGIRKRSPTGKLIFGSVARDVILKANKPVICIK 134
emb|CAB53422.1| (AL109989) hypothetical protein SCJ12.10c [Streptomyces coelicolor
A3(2)]
Length = 288
Score = 66.2 bits (159), Expect = 1e-10
Identities = 27/155 (17%), Positives = 60/155 (38%), Gaps = 16/155 (10%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MY ++ D SE++ A+ L + ++H + + + LG +
Sbjct: 1 MYLPMVVGVDGSESSLGAVDWAADEAALHEVPLRIVHAYR-WDRYEGASLARELGKPSGH 59
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
+ ++ + ++ D+ + P +++ A +
Sbjct: 60 VTTDDILAVATRR-----------ARRHHPDLAVTTEATAEE--PEYVLLREARN--ASA 104
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+I+G+ G+ L +LLGSV+ V S+ PV+V +
Sbjct: 105 VILGTRGRGELAGLLLGSVSLTVATMSDCPVVVTR 139
Score = 42.4 bits (98), Expect = 0.002
Identities = 24/151 (15%), Positives = 49/151 (31%), Gaps = 20/151 (13%)
Query: 7 KILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSV 66
+I+ + TA A++ + L + D L
Sbjct: 154 RIVVGVADAPTA--AVRFACEEARRRGA---ALDAVRAWRCPTHDTVD-----HPLLAGT 203
Query: 67 EEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIM 126
E +E + +E + + + +++ G P ++ A E D++++
Sbjct: 204 PERLHE--ERAAKELEAALADA-----PADVRLRRRTAEG-PGSRVLSAASHE-ADLLVV 254
Query: 127 GSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
G + LG V ++ S PV VV
Sbjct: 255 GRR-RPGQFGHRLGRVAHTLLHHSACPVAVV 284
emb|CAB63186.1| (AL133469) hypothetical protein SCM10.25 [Streptomyces coelicolor
A3(2)]
Length = 153
Score = 65.8 bits (158), Expect = 2e-10
Identities = 30/152 (19%), Positives = 62/152 (40%), Gaps = 16/152 (10%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
K I+ D S+++ A + + + +++V L G A L S
Sbjct: 17 KVIVVGVDGSDSSLRAAAYAGGMARRQGALLAVVYVQPV-----------LAGGAALGAS 65
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIII 125
V E +E+ +L + + E +K + + + G P+ + + A++ D ++
Sbjct: 66 VAETTDEIAEELVAQIREATERVKGIFD---IRWEFHTFRGDPYSGLRQTADELKADAVV 122
Query: 126 MGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
+G+ + +GSV ++K PV VV
Sbjct: 123 VGASEQAG--HRFVGSVAVRLVKAGRWPVTVV 152
gi|2648945 (AE000991) cationic amino acid transporter (cat-1) [Archaeoglobus
fulgidus]
Length = 736
Score = 64.2 bits (154), Expect = 5e-10
Identities = 31/155 (20%), Positives = 64/155 (41%), Gaps = 24/155 (15%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
M IL P A+ ++ + K V++L+ + + ++
Sbjct: 468 MEYTILVPVANPVIAKKLVRFAELIARKKKGAVVILNTVRLPQQ------------TPIS 515
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDI 123
++ ++AK +E + G VK V E I+ AE+ ++
Sbjct: 516 APAKDV---------KKAKELVEGLMNLSVPSGGVVK---VSHSVSEAILSTAEEWKANM 563
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
I+MG G+T ++++LGS + V+ K+ V+V++
Sbjct: 564 IVMGWRGRTFRRDVVLGSTIDPVLLKAKCDVVVIR 598
Score = 52.9 bits (125), Expect = 1e-06
Identities = 33/156 (21%), Positives = 61/156 (38%), Gaps = 35/156 (22%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
++ +L T A++ + +A K+ + LL+V
Sbjct: 608 FRDVLISTIGGPHAKLGYEIARALVEDKSGRIKLLYVGSS-------------------- 647
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDII-VVGIPHEEIVKIAEDEGVDI 123
E E E K+ EEA + G V+ + P + + + AE D+
Sbjct: 648 ---EKEREKAEKVFEEAMEILN---------GLNVEREFAISSSPSDVVAREAES--FDL 693
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
+I+G+ +T LK L G E V+ K++K V + ++
Sbjct: 694 VIVGASERTFLKNFLTGLFPEKVVMKTSKTVAMTRK 729
gi|2649038 (AE000997) conserved hypothetical protein [Archaeoglobus fulgidus]
Length = 145
Score = 63.1 bits (151), Expect = 1e-09
Identities = 38/159 (23%), Positives = 71/159 (43%), Gaps = 22/159 (13%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKT-LKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
++IL D + EIA + +K EV +L++ + + V
Sbjct: 2 ERILLVLDDTGRGEIAFQKLKKLAEDGLRGEVYILYIRE-------------MEVPPFV- 47
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDI-IVVGIPHEEIVKIAEDEGVDI 123
EE E ++L ++ K+E K +LE G KV D+ +V G + ++ + + D+
Sbjct: 48 -PEEKELAAYHRLMTQSMKKLEGFKNQLEKAGLKVSDVSVVFGKYADRLLLVEKQIKPDL 106
Query: 124 IIMGSHGKTNLKEILLG----SVTENVIKKSNKPVLVVK 158
I++G G LK ++ G E ++KKS +L+ +
Sbjct: 107 IVVGFKG-GLLKRVIGGFFGKDPCEVLLKKSKASLLICR 144
emb|CAA21268| (AL031853) hypothetical protein [Schizosaccharomyces pombe]
Length = 307
Score = 60.7 bits (145), Expect = 6e-09
Identities = 35/157 (22%), Positives = 65/157 (41%), Gaps = 21/157 (13%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
+ L D + +E+A+ + +E ++L VID + L +S
Sbjct: 140 RTFLCGMDGNSYSEVAVDWLFETLLADNDEAVVLRVIDP-----SSKLAEDLSDEQSYRS 194
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIII 125
+ E +KK +D + +VVG P + I++ D +I
Sbjct: 195 LAEHIMA-------------GILKKVDDDKAVSIIVELVVGKPQDMILRTIHVYSPDSLI 241
Query: 126 MGSHGKTN--LKEILL-GSVTENVIKKSNKPVLVVKR 159
+G+ GK + +L GSV++ ++KS PV+VV+
Sbjct: 242 VGTRGKALNSFQSLLSSGSVSKFCLQKSPIPVIVVRP 278
emb|CAB53431.1| (AL109989) hypothetical protein SCJ1.19c [Streptomyces coelicolor
A3(2)]
Length = 201
Score = 60.3 bits (144), Expect = 8e-09
Identities = 29/151 (19%), Positives = 60/151 (39%), Gaps = 19/151 (12%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
I D S + A + + L+HV R L V G ++
Sbjct: 5 IAVGIDRSPASLAAAHWAAHEARRRGSGITLVHVWHRRARPT-----PYLRVGGTERAWA 59
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
E + EEA + + G ++ + +V +V A+ +++++G
Sbjct: 60 E-------RTLEEAVRSVRSA-----HPGLRITERLVCDATVTALVTAAD--DAEMLVLG 105
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
S G + + GSV++ V+ +++ PV++V+
Sbjct: 106 SPGPGPVGGFVTGSVSQRVVARADHPVVLVR 136
emb|CAA14661| (AJ235270) unknown [Rickettsia prowazekii]
Length = 148
Score = 59.6 bits (142), Expect = 1e-08
Identities = 32/158 (20%), Positives = 70/158 (44%), Gaps = 22/158 (13%)
Query: 5 YKKILYPTDFSETAEIALKH----VKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
+K IL P D ++ + ++K ++ ++VI E F +
Sbjct: 7 FKNILIPIDLND--KKSIKSIFPKALMLAINFQAKLHFMYVIPE--------FGTKMFED 56
Query: 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEG 120
L K+ + E+ + ++++I K+ + + I G ++EI+K + +
Sbjct: 57 YLPKNWRI-------EKKEKYQTQIKDIIKQYIPDTIETDNYIGSGAVYDEIIKRSNEIK 109
Query: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
D+II+ S + LK+ +LG +++ S+ VLV++
Sbjct: 110 ADLIII-SAVRLQLKDYMLGPNASKIVRHSDISVLVLR 146
dbj|BAA86264.1| (AB023785) ORF2 [Streptomyces griseus]
Length = 173
Score = 59.2 bits (141), Expect = 2e-08
Identities = 23/150 (15%), Positives = 54/150 (35%), Gaps = 17/150 (11%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
++ D S ++E AL + +I++HV + + V
Sbjct: 40 VVVGFDGSTSSERALAYAIGMAGRSGSGLIIVHVANRLPTTVWAGC-----EPPVFVDVP 94
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
+ E+ L + + + + G E+ ++ + D I++G
Sbjct: 95 DHRTEVLG-LELACADYLSD---------VPWVLVERGGDICHELEEVGREYAADAIVVG 144
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
S + + GSV + +++ +PV+V+
Sbjct: 145 ST--HGIVGRIFGSVAGRLARRAQRPVIVI 172
pir||G64029 hypothetical protein HI1426 - Haemophilus influenzae (strain Rd
KW20) >gi|1574260 (U32821) conserved hypothetical
protein [Haemophilus influenzae Rd]
Length = 340
Score = 56.8 bits (135), Expect = 9e-08
Identities = 24/158 (15%), Positives = 64/158 (40%), Gaps = 9/158 (5%)
Query: 2 SVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAG 61
+ +K IL + S + AL + E + ++ L ++
Sbjct: 31 IMKFKNILVVLNPSNEKQYALARAVRLVEEQKNE------TKVKITALLSVYDLSYEMSA 84
Query: 62 LNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVG-IPHEEIVKIAEDEG 120
L S E +E+ ++ E+ ++ ++ + + +++ IV + I + E+
Sbjct: 85 LLSS--EERSEMHQQVIEKHRHAVQYYLDKYANPEIELQSHIVWNSNEADAINEEVENNN 142
Query: 121 VDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
D+++ + + L ++ + +++K PVL+V+
Sbjct: 143 YDLVVKYTKDEEKLTSLIFTPIDWQLLRKCPIPVLMVR 180
Score = 54.9 bits (130), Expect = 3e-07
Identities = 19/59 (32%), Positives = 35/59 (59%)
Query: 101 DIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
+ G P E I ++A++ +++I+G+ G+T L LLG+ E+VI K + +L +K
Sbjct: 277 THVREGFPEEVIPEVAKEIEAELVILGTVGRTGLSAALLGNTAEHVISKLSCNLLGIKP 335
sp|P44195|YDAA_HAEIN HYPOTHETICAL PROTEIN HI1426
Length = 309
Score = 56.4 bits (134), Expect = 1e-07
Identities = 24/155 (15%), Positives = 63/155 (40%), Gaps = 9/155 (5%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
+K IL + S + AL + E + ++ L ++ L
Sbjct: 3 FKNILVVLNPSNEKQYALARAVRLVEEQKNE------TKVKITALLSVYDLSYEMSALLS 56
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVG-IPHEEIVKIAEDEGVDI 123
S E +E+ ++ E+ ++ ++ + + +++ IV + I + E+ D+
Sbjct: 57 S--EERSEMHQQVIEKHRHAVQYYLDKYANPEIELQSHIVWNSNEADAINEEVENNNYDL 114
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
++ + + L ++ + +++K PVL+V+
Sbjct: 115 VVKYTKDEEKLTSLIFTPIDWQLLRKCPIPVLMVR 149
Score = 54.9 bits (130), Expect = 3e-07
Identities = 19/59 (32%), Positives = 35/59 (59%)
Query: 101 DIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
+ G P E I ++A++ +++I+G+ G+T L LLG+ E+VI K + +L +K
Sbjct: 246 THVREGFPEEVIPEVAKEIEAELVILGTVGRTGLSAALLGNTAEHVISKLSCNLLGIKP 304
gi|1002877 (U34353) ORF278 [Paracoccus denitrificans]
Length = 278
Score = 54.9 bits (130), Expect = 3e-07
Identities = 18/74 (24%), Positives = 41/74 (55%), Gaps = 3/74 (4%)
Query: 88 IKKELEDVGFKVKDIIVVGI---PHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTE 144
+ + L G K + ++ + + + A + G D+++MG++G + +E +LG T
Sbjct: 205 LCQMLTRHGVKAEISVLARTLPLISDILNRRATEIGADMLVMGAYGHSRFREAILGGATR 264
Query: 145 NVIKKSNKPVLVVK 158
N+++K+ PVL+ +
Sbjct: 265 NMLEKAQVPVLMAR 278
sp|P44880|USPA_HAEIN UNIVERSAL STRESS PROTEIN A HOMOLOG >gi|1075424|pir||A64096
universal stress protein (uspA) homolog - Haemophilus
influenzae (strain Rd KW20) >gi|1573828 (U32764)
universal stress protein A (uspA) [Haemophilus
influenzae Rd]
Length = 141
Score = 54.1 bits (128), Expect = 6e-07
Identities = 27/155 (17%), Positives = 67/155 (42%), Gaps = 20/155 (12%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MYK IL D SE + I LK ++ ++HV ++ D++
Sbjct: 1 MYKHILVAVDLSEESPILLKKAVGIAKRHDAKLSIIHV----DVNFSDLY---------T 47
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIV-VGIPHEEIVKIAEDEGVD 122
++ + ++++++ E + + ++ + ++ + + + + G + + E VD
Sbjct: 48 GLIDVNMSSMQDRISTETQKALLDLAESVD---YPISEKLSGSGDLGQVLSDAIEQYDVD 104
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
+++ G H + +++ S T V+ +LVV
Sbjct: 105 LLVTGHH-QDFWSKLM--SSTRQVMNTIKIDMLVV 136
emb|CAB40780.1| (AL049608) hypothetical protein [Arabidopsis thaliana]
Length = 219
Score = 53.7 bits (127), Expect = 8e-07
Identities = 40/186 (21%), Positives = 69/186 (36%), Gaps = 30/186 (16%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDER----------EIKKRDIFSL 55
+KI+ D + + AL++ + L+ +E+IL+H+ + I S
Sbjct: 15 RKIMVIADPTRESAAALQYALSHAVLEQDELILVHIENSGGSWKNAFSSFLRLPSSISSS 74
Query: 56 LLGVAGLNKSVEEFENELKNKLTEEAKN----KMENIKKELEDVGFKVKD----IIVVGI 107
G + + N N L E +E +K+ E KV+ I + G+
Sbjct: 75 SSGSSPASNGTTTASNAAANALASEIGQGDGNFLEQMKRICEIAQPKVRVHTECIAIDGV 134
Query: 108 PHEEIVKIAEDEGVDIIIMGSH--------GKTNLKEILLGS----VTENVIKKSNKPVL 155
I+ + GVD+II+G G L GS E +I+ S +
Sbjct: 135 KATAILLHGDKLGVDVIIIGQRRTISSSLLGTRRPGGSLRGSKGVDTAEYLIENSKCTCV 194
Query: 156 VVKRKN 161
V +K
Sbjct: 195 GVTKKG 200
sp|P03807|YDAA_ECOLI 35.6 KD PROTEIN IN TPX-FNR INTERGENIC REGION
>gi|1742190|dbj|BAA14926| (D90771) ORF_ID:o261#5;
similar to [SwissProt Accession Number P44195]
[Escherichia coli] >gi|1742201|dbj|BAA14936| (D90772)
ORF_ID:o261#5; similar to [SwissProt Accession Number
P44195] [Escherichia coli] >gi|1787594 (AE000231) orf,
hypothetical protein [Escherichia coli]
Length = 316
Score = 53.7 bits (127), Expect = 8e-07
Identities = 18/59 (30%), Positives = 30/59 (50%)
Query: 101 DIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
+ G+P E I +AE I+++G+ G+T + LG+ E VI +LV+K
Sbjct: 243 THVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGISAAFLGNTAEQVIDHLRCDLLVIKP 301
Score = 49.0 bits (115), Expect = 2e-05
Identities = 24/156 (15%), Positives = 57/156 (36%), Gaps = 13/156 (8%)
Query: 4 MYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLN 63
MY+ +L D ++ + AL+ ++ I+ + L
Sbjct: 3 MYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAF----------LPIYDFSYEMTTLL 52
Query: 64 KSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVV-GIPHEEIVKIAEDEGVD 122
E ++ + + + K + G ++ +V P E I++ G D
Sbjct: 53 SPDER--TAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQEVISGGHD 110
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVK 158
+++ +H L+ ++ ++++K PV +VK
Sbjct: 111 LVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVK 146
emb|CAB06280| (Z83867) hypothetical protein Rv3134c [Mycobacterium tuberculosis]
Length = 268
Score = 53.3 bits (126), Expect = 1e-06
Identities = 24/156 (15%), Positives = 55/156 (34%), Gaps = 27/156 (17%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
+ ++ D S A A + + L++VID ++
Sbjct: 8 RAVVVGIDGSRAATHAALWAVDEAVNRDIPLRLVYVIDPSQLSAAGEGGG---------- 57
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVG--FKVKDIIVVGIPHEEIVKIAEDEGVDI 123
A+ + + +++E G K++ ++ G P ++ + E +
Sbjct: 58 ------------QSAARAALHDASRKVEATGQPVKIETEVLCGRPLTKL--MQESRSAAM 103
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
+ +GS G +++ GSV + + PV V+
Sbjct: 104 LCVGSVGLDHVRGR-RGSVAATLAGSALCPVAVIHP 138
gb|AAC67211.1| (AC005171) putative protein kinase [Arabidopsis thaliana]
Length = 620
Score = 52.2 bits (123), Expect = 2e-06
Identities = 30/159 (18%), Positives = 60/159 (36%), Gaps = 19/159 (11%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
+ D + ++ ALK E + L+HV ++ + G V+
Sbjct: 11 VAIAIDRDKGSQAALKWAVDNLLTPGETLTLIHVKVKQTLANNGTQPNKSG-----DDVK 65
Query: 68 E----FENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVG-IPHEEIVKIAEDEGVD 122
E F K A N + +K +++++ E I++ ++ +D
Sbjct: 66 ELFLPFRCFCTRKDVSFASNFINLLK-------INCEEVVLENVDAAEGIIEYVQENAID 118
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSN--KPVLVVKR 159
I+++G+ T LK + VT VIK + V + +
Sbjct: 119 ILVLGASKITLLKRLKAVDVTNAVIKGAPNFCTVYAISK 157
gb|AAF27072.1|AC008262_21 (AC008262) F4N2.5 [Arabidopsis thaliana]
Length = 223
Score = 51.8 bits (122), Expect = 3e-06
Identities = 30/170 (17%), Positives = 59/170 (34%), Gaps = 24/170 (14%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
++I+ D A+ AL + + ++LLH + + + D L
Sbjct: 44 RRIIVVVDSCSEAKNALLWTLSHCAQPQDSILLLHFLKAKTSQSGD----------LANK 93
Query: 66 VEEFENELKNKLTEEAKNKMENIKK--ELEDVGFKVKDIIVVGIPHE-EIVKIAEDEGVD 122
E + T A K+ +K EL+ K + + V G IVK A +
Sbjct: 94 EEGEDESCDKPTTSRADKKVSALKTMCELKRPEVKTEVVFVKGDEKGPTIVKEAREREAS 153
Query: 123 IIIMGSHGKTNLKEILL-----------GSVTENVIKKSNKPVLVVKRKN 161
++++G + +L+ E I S + V+++
Sbjct: 154 LLVLGQKKQHATWRLLMVWASQARPVTKHDFVEYCINNSPCMAIAVRKRG 203
emb|CAA17242| (AL021899) hypothetical protein Rv2028c [Mycobacterium
tuberculosis]
Length = 279
Score = 51.4 bits (121), Expect = 4e-06
Identities = 24/152 (15%), Positives = 50/152 (32%), Gaps = 19/152 (12%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
I+ D S+ A A + + LL+ I+ + G A +
Sbjct: 10 IVVGIDGSKPAVQAALWAVDEAASRDIPLRLLYAIEPDDP----------GYAAHGAAAR 59
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
+ E + E D KV+ I P +++ + ++ +G
Sbjct: 60 KLAAA-------ENAVRYAFTAVEAADRPVKVEVEITQERPVTSLIRAS--AAAALVCVG 110
Query: 128 SHGKTNLKEILLGSVTENVIKKSNKPVLVVKR 159
+ G + + +GS + + PV +V+
Sbjct: 111 AIGVHHFRPERVGSTAAALALSAQCPVAIVRP 142
gi|2338738 (AF016223) unknown [Rhodobacter capsulatus]
Length = 279
Score = 51.4 bits (121), Expect = 4e-06
Identities = 28/154 (18%), Positives = 61/154 (39%), Gaps = 35/154 (22%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
++++ + S A A++ KA L+A ++ + VID
Sbjct: 156 RRVVVAWNQSNEAMNAIR--KALPLLQAATLVDITVIDPPA------------------- 194
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGI---PHEEIVKIAEDEGVD 122
E + + + L G KV+ ++ + I + D +
Sbjct: 195 -----------HGPERSDPGGQLSQWLARHGVKVEVSVLAKTLPRISDVINRHVRDTSAE 243
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLV 156
+++MG++G + +E +LG T N+++ + PVL+
Sbjct: 244 MVVMGAYGHSRFREAILGGATRNMLEMAEVPVLM 277
emb|CAA89821.2| (Z49746) ORF277 [Rhodobacter sphaeroides]
Length = 79
Score = 51.0 bits (120), Expect = 5e-06
Identities = 15/72 (20%), Positives = 38/72 (51%), Gaps = 3/72 (4%)
Query: 88 IKKELEDVGFKVKDIIVVGI---PHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTE 144
+ + L G + + ++ + I + D+ D+++MG++G + +E +LG T
Sbjct: 6 LCQMLVRHGVRAEVSVLAKTMPRISDVIARHVRDQDADLLVMGAYGHSRFREAILGGATR 65
Query: 145 NVIKKSNKPVLV 156
++++ + PVL+
Sbjct: 66 DMLELAEVPVLM 77
gi|2982911 (AE000677) putative protein [Aquifex aeolicus]
Length = 135
Score = 51.0 bits (120), Expect = 5e-06
Identities = 23/120 (19%), Positives = 48/120 (39%), Gaps = 10/120 (8%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
K +L TD E A+ + F E+ +L V++ ++ + ++ G+
Sbjct: 2 KVLLVLTDAYSDCEKAITYAVNFSEKLGAELDILAVLE--DVYNLERANVTFGLPF---- 55
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIII 125
E + E K ++ + E + E G +G EE+ K E +G ++++
Sbjct: 56 PPEIKEESKKRIERRLREVWEKLTGSTEIPGV----EYRIGPLSEEVKKFVEGKGYELVV 111
sp|P46888|YECG_ECOLI HYPOTHETICAL 17.1 KD PROTEIN IN FLHD-OTSA INTERGENIC REGION
>gi|862972 (U27211) similar to Escherichia coli
universal stress protein A, Swiss-Prot Accession Number
P28242 [Escherichia coli] >gi|1736554|dbj|BAA15716|
(D90831) Universal stress protein A [Escherichia coli]
>gi|1788205 (AE000283) putative regulator [Escherichia
coli]
Length = 142
Score = 49.8 bits (117), Expect = 1e-05
Identities = 29/154 (18%), Positives = 58/154 (36%), Gaps = 20/154 (12%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
Y IL + ++ L + + L+ + + E+ L
Sbjct: 3 YSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPEMYN------QLAAP---- 52
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKV-KDIIVVGIPHEEIVKIAEDEGVDI 123
+L++ + EE ++ ++ K ++D G+ V K I G E I+++ D+
Sbjct: 53 ----MLEDLRSVMHEETQSFLD---KLIQDAGYPVDKTFIAYGELSEHILEVCHKHHFDL 105
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
+I G+H + + VI S VL+V
Sbjct: 106 VICGNHNHSFFSR--ASCSAKRVIASSEVDVLLV 137
gb|AAD20074.1| (AC006836) unknown protein [Arabidopsis thaliana]
Length = 165
Score = 49.0 bits (115), Expect = 2e-05
Identities = 31/165 (18%), Positives = 58/165 (34%), Gaps = 33/165 (20%)
Query: 9 LYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVEE 68
+ D + + AL+ + + LLHV V +
Sbjct: 1 MVVVDTTSQTKNALQWALTHCVQDEDNITLLHVTRTP--------------------VGQ 40
Query: 69 FENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEE----IVKIAEDEGVDII 124
+E + + A + +K + VK IVV EE IV+ ++ +G ++
Sbjct: 41 AIDETQRERNSRAHELVHPLKNFCQLKKPNVKTEIVVVETAEEKGKTIVEESKKQGAGVL 100
Query: 125 IMGSHGKT---------NLKEILLGSVTENVIKKSNKPVLVVKRK 160
++G +T K + G V E I S+ + V++K
Sbjct: 101 VLGQRKRTSKWRVIWKWRTKGGMGGGVVEYCIHNSDCMAIAVRKK 145
gb|AAD23643.1|AC007119_9 (AC007119) unknown protein [Arabidopsis thaliana]
Length = 184
Score = 48.3 bits (113), Expect = 3e-05
Identities = 26/130 (20%), Positives = 44/130 (33%), Gaps = 20/130 (15%)
Query: 8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVE 67
++ D ++ A A+ + L+H + FSL N V
Sbjct: 42 VIVAVDHGPNSKHAFDWALVHFCRLADTLHLVHAV-------SSSFSLQCVK---NDVVY 91
Query: 68 EFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMG 127
E L KL EA K +V G + I K AE +I+G
Sbjct: 92 ETSQALMEKLAVEAYQV----------AMVKSVARVVEGDAGKVICKEAEKVKPAAVIVG 141
Query: 128 SHGKTNLKEI 137
+ G++ ++ +
Sbjct: 142 TRGRSLVRRL 151
sp|P28242|USPA_ECOLI UNIVERSAL STRESS PROTEIN A >gi|281977|pir||S28016 universal stress
protein A - Escherichia coli >gi|43280|emb|CAA47884|
(X67639) universal stress protein A (UspA) [Escherichia
coli]
Length = 144
Score = 48.3 bits (113), Expect = 3e-05
Identities = 28/154 (18%), Positives = 64/154 (41%), Gaps = 20/154 (12%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
YK IL D S +++ ++ + +V L+HV ++ D+++ L+ V L
Sbjct: 3 YKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHV----DVNYSDLYTGLIDV-NLGD 57
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKV-KDIIVVGIPHEEIVKIAEDEGVDI 123
+ E + LTE + G+ + + + G + +V + +D+
Sbjct: 58 MQKRISEETHHALTE-----------LSTNAGYPITETLSGSGDLGQVLVDAIKKYDMDL 106
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++ G H + +++ S +I + +L+V
Sbjct: 107 VVCGHH-QDFWSKLM--SSARQLINTVHVDMLIV 137
pir||S47715 uspA protein - Escherichia coli >gi|466632 (U00039) uspA
[Escherichia coli] >gi|1789909 (AE000425) universal
stress protein; broad regulatory function? [Escherichia
coli]
Length = 144
Score = 48.3 bits (113), Expect = 3e-05
Identities = 28/154 (18%), Positives = 64/154 (41%), Gaps = 20/154 (12%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
YK IL D S +++ ++ + +V L+HV ++ D+++ L+ V L
Sbjct: 3 YKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHV----DVNYSDLYTGLIDV-NLGD 57
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKV-KDIIVVGIPHEEIVKIAEDEGVDI 123
+ E + LTE + G+ + + + G + +V + +D+
Sbjct: 58 MQKRISEETHHALTE-----------LSTNAGYPITETLSGSGDLGQVLVDAIKKYDMDL 106
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++ G H + +++ S +I + +L+V
Sbjct: 107 VVCGHH-QDFWSKLM--SSARQLINTVHVDMLIV 137
gi|3493653 (AF083219) unknown [Azospirillum brasilense]
Length = 280
Score = 47.9 bits (112), Expect = 4e-05
Identities = 13/50 (26%), Positives = 31/50 (62%)
Query: 107 IPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLV 156
P + ++ DE D+++MG++ ++ ++E +LG +T +++ PVL+
Sbjct: 229 DPGDTLLNTVADESCDLLVMGAYARSRVREQVLGGMTRYMLEHMTVPVLM 278
Alignment 1
dbj|BAA10378| (D64002) Na(+)/H(+) antiporter [Synechocystis sp.]
Length = 698
Score = 47.5 bits (111), Expect = 6e-05
Alignment 2
Score = 30.7 bits (68), Expect = 6.4
dbj|BAA85313.1| (D43640) UspA analogue [Salmonella typhimurium]
Length = 142
Score = 45.1 bits (105), Expect = 3e-04
Identities = 25/154 (16%), Positives = 57/154 (36%), Gaps = 20/154 (12%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
Y IL + + L + +V L+ + + E+ + ++
Sbjct: 3 YTHILVAVAVTPESHQLLAKAVSIARPVQAKVSLITLASDPELYNQFAAPMM-------- 54
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDI-IVVGIPHEEIVKIAEDEGVDI 123
+L+ + EE +N ++ + E + ++ I G + I+ + VD+
Sbjct: 55 ------EDLRAVMHEETENFLKML---GEKADYPIEQTFIASGELSQHILAVCRKHHVDL 105
Query: 124 IIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
+I G+H + ++V+ S VL+V
Sbjct: 106 VICGNHNHSFFSR--ASCSAKSVVSASQVDVLLV 137
gi|2983091 (AE000689) putative protein [Aquifex aeolicus]
Length = 439
Score = 44.8 bits (104), Expect = 4e-04
Identities = 26/153 (16%), Positives = 57/153 (36%), Gaps = 27/153 (17%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
Y+K+L + ++ E+ L ++ +L+V K
Sbjct: 307 YEKVL-ASANTDAREVLLSTAVDIAVQFGSKLFILYVKP------------------FEK 347
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDII 124
+ E E E+ L A ++ I K ++ +I G P E +K ++E +++
Sbjct: 348 MISEKEKEVIEGLKSFA-ERVRKITK------INLELVIREGNPVRETLKFMDEESFNLL 400
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
++G + ++ + +KS L+V
Sbjct: 401 LIG-YKVGRTTKLFSPYSPHLIARKSKLSTLLV 432
gb|AAF26099.1|AC012328_2 (AC012328) hypothetical protein [Arabidopsis thaliana]
Length = 274
Score = 44.0 bits (102), Expect = 7e-04
Identities = 34/174 (19%), Positives = 65/174 (36%), Gaps = 35/174 (20%)
Query: 16 ETAEIALKHVKAFKTLKAEEVILLHVID---------EREIKKR----DIFSLLLGVAGL 62
E E A++ V+A T V++ V+D E +K D LL
Sbjct: 88 EETEAAVEAVEAEITEAGNRVMV--VVDKVIASTGALEWALKHTLQSQDYLFLLYFSKPF 145
Query: 63 NKSVEEFENELKNKLTEEAKNKMENIKKELE--DVGFKVKDIIVVGIPHEE---IVKIAE 117
K K + + +KK + G +V+ + G E+ IV+ A+
Sbjct: 146 RKG-----KRKNRKSEVKTDELVHTLKKLCQTKRPGIEVEIRRLQGKEKEKGEKIVEEAK 200
Query: 118 DEGVDIIIMGSHGKTNLKEIL----------LGSVTENVIKKSNKPVLVVKRKN 161
++ V ++++G K + +L + ++K++ + VK KN
Sbjct: 201 EQQVSLLVVGKEKKPPVWRLLKRWGWKKRRGRAGTLKYCLEKASCMTIAVKPKN 254
sp|P71893|YN19_MYCTU HYPOTHETICAL 31.9 KD PROTEIN RV2319C >gi|1524281|emb|CAB02071|
(Z79702) hypothetical protein Rv2319c [Mycobacterium
tuberculosis]
Length = 292
Score = 44.0 bits (102), Expect = 7e-04
Identities = 19/95 (20%), Positives = 41/95 (43%), Gaps = 3/95 (3%)
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGF---KVKDIIVVGIPHEEIVKIAEDEGVD 122
E + + E + + ++L G V +V G + + A+ + +
Sbjct: 196 PPEVGLHAEASVLEAWAAQARELLEKLRINGVVSEDVVLQVVTGNGWAQALDAADWQDGE 255
Query: 123 IIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
I+ +G+ ++ + LGS + +I+ S PVLV+
Sbjct: 256 ILALGTSPFGDVARVFLGSWSGKIIRYSPVPVLVL 290
Score = 42.8 bits (99), Expect = 0.001
Identities = 9/45 (20%), Positives = 24/45 (53%)
Query: 112 IVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLV 156
++ + E+ +++++GS +L+GS + ++ S PV +
Sbjct: 95 LLDVVEELEAEVLVLGSFPSGRRARVLIGSTADRLLHSSPVPVAI 139
sp|P32163|YIIT_ECOLI HYPOTHETICAL 16.3 KD PROTEIN IN TPIA-FPR INTERGENIC REGION
>gi|541113|pir||S40866 hypothetical protein o142 -
Escherichia coli >gi|305026 (L19201) ORF_o142
[Escherichia coli] >gi|1790358 (AE000467) putative
regulator [Escherichia coli]
Length = 142
Score = 44.0 bits (102), Expect = 7e-04
Identities = 28/153 (18%), Positives = 58/153 (37%), Gaps = 18/153 (11%)
Query: 5 YKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNK 64
YK I +E + + + L+H+ D L G+
Sbjct: 3 YKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDD----------GLSELYPGIYF 52
Query: 65 SVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDII 124
E ++ L ++ NK+ + K ++ K K I G E +++I + E D++
Sbjct: 53 PATE---DILQLLKNKSDNKLYKLTKNIQWP--KTKLRIERGEMPETLLEIMQKEQCDLL 107
Query: 125 IMGSHGKTNLKEILLGSVTENVIKKSNKPVLVV 157
+ G H + + ++ +I K + +L+V
Sbjct: 108 VCGHH-HSFINRLM--PAYRGMINKMSADLLIV 137
gi|152197 (L07487) DNA-region 1 to 268 is overlapping with fixLJ-region
(EMBLAccession no. X56808).; putative [Bradyrhizobium
japonicum] >gi|3021312|emb|CAA06277| (AJ005001)
hypothetical protein [Bradyrhizobium japonicum]
Length = 277
Score = 43.6 bits (101), Expect = 9e-04
Identities = 27/151 (17%), Positives = 62/151 (40%), Gaps = 27/151 (17%)
Query: 6 KKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKS 65
+ +L + A A+ A L+ + + I ER+ + + + GV +
Sbjct: 152 RSVLVAWKDTPEARRAV--ADALPMLRKARDVTIAAIPERDDDRSVVMA---GVTDVAAW 206
Query: 66 VEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIII 125
+ +++E A + E +++ + A D G +I+
Sbjct: 207 LARHGVTATARVSEAAGD---------EPAAAQLEQV-------------AGDVGAGLIV 244
Query: 126 MGSHGKTNLKEILLGSVTENVIKKSNKPVLV 156
G++G + +E++LG VT+ ++ +S + VL+
Sbjct: 245 AGAYGHSRFRELILGGVTQYLVTQSARSVLL 275
dbj|BAA88920.1| (AB023410) cyst specific protein CSP 21 [Acanthamoeba castellanii]
Length = 175
Score = 42.8 bits (99), Expect = 0.001
Identities = 25/156 (16%), Positives = 56/156 (35%), Gaps = 29/156 (18%)
Query: 9 LYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVEE 68
+ D + + AL+ ++L+H + +G EE
Sbjct: 40 VVCVDDLKESRRALRFALKNVPRNH-RLLLVH----------GKYEGRIGGER--AMPEE 86
Query: 69 FENELKNKLTEEAKNKMENIKKELE--DVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIM 126
+ EE +K E + D G + G ++ ++AE +I+
Sbjct: 87 IRSRFLRMCKEEG------MKCEFKIFDYGSNRE----FGD---KVCRLAERNNAKSVII 133
Query: 127 GSH-GKTNLKEILLGSVTENVIKKSNKPVLVVKRKN 161
G ++ + ++GS +++V+ PV +V+ +
Sbjct: 134 GKREDVSDTRRAIIGSSSQSVLSSCGMPVTIVQSEG 169
Revised May 28, 2000
) that are better than the specified E value threshold. Although the majority of these hits are unannotated, several have known functions or resemble proteins with known functions. Annotated hits are shown in bold in this tutorial. To further evaluate the annotated hits, examine each alignment. Click on the corresponding score to jump down to the alignment for each annotated hit in the output of the first iteration.
- alignment was checked on the previous iteration
Score E
Sequences producing significant alignments: