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1: J Pharm Biomed Anal. 2007 Sep 3;44(5):1095-9. Epub 2007 Apr 22.Click here to read Links

Gas chromatographic-mass spectrometric analysis of d-limonene in human plasma.

Wang S, Chen Y, Gao Z, Xiong M, Zhong Z, Ye L.

Department of Pharmacy, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, PR China.

d-Limonene shows carminative and cholagogue effects and is used in treatment of gallstone, cholecystitis and angiocholitis. A simple method was developed to determine the concentration of d-limonene in human plasma. d-Limonene and internal standard (naphthalane, C(10)H(18)) were extracted with n-hexane and then injected to GC-MS. Calibration curves were linear (r=0.9990, n=6) in the range of 2-500 ng/ml for d-limonene in human plasma. Limit of detection and quantification were 0.5 and 2 ng/ml, respectively. This rapid and specific method was applied to the clinical pharmacokinetic investigation of d-limonene.

PMID: 17540528 [PubMed - indexed for MEDLINE]

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