BLASTP 2.2.18 [Mar-02-2008]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= SPy_0448 hypothetical protein
(113 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
6,515,104 sequences; 2,222,278,849 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_268745.1| hypothetical protein SPy_0448 [Streptococc... 226 5e-58
ref|YP_059708.1| hypothetical protein M6_Spy0390 [Streptoco... 203 3e-51
ref|YP_279824.1| hypothetical protein M28_Spy0355 [Streptoc... 201 9e-51
ref|YP_597978.1| hypothetical protein MGAS10270_Spy0369 [St... 194 2e-48
ref|NP_664120.1| hypothetical protein SpyM3_0316 [Streptoco... 179 7e-44
ref|YP_601862.1| hypothetical protein MGAS10750_Spy0368 [St... 177 3e-43
ref|NP_606714.1| hypothetical protein spyM18_0491 [Streptoc... 155 9e-37
ref|YP_596101.1| hypothetical protein MGAS9429_Spy0369 [Str... 129 6e-29
ref|YP_599981.1| hypothetical protein MGAS2096_Spy0385 [Str... 127 2e-28
ref|YP_599373.1| hypothetical protein MGAS10270_Spy1764 [St... 36 0.73
ref|XP_001308072.1| conserved hypothetical protein [Trichom... 35 1.3
gb|AAM37977.1| chemotaxis protein [Xanthomonas axonopodis p... 33 4.0
ref|NP_643441.2| chemotaxis protein [Xanthomonas axonopodis... 33 4.1
ref|YP_364992.1| methyl-accepting chemotaxis protein [Xanth... 33 4.1
>ref|NP_268745.1| hypothetical protein SPy_0448 [Streptococcus pyogenes M1 GAS]
ref|YP_281729.1| hypothetical protein M5005_Spy_0366 [Streptococcus pyogenes
MGAS5005]
gb|AAK33466.1| hypothetical protein SPy_0448 [Streptococcus pyogenes M1 GAS]
gb|AAZ50984.1| hypothetical protein M5005_Spy0366 [Streptococcus pyogenes
MGAS5005]
Length = 113
Score = 226 bits (575), Expect = 5e-58, Method: Compositional matrix adjust.
Identities = 113/113 (100%), Positives = 113/113 (100%)
Query: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL 60
MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL
Sbjct: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL 60
Query: 61 RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY
Sbjct: 61 RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
>ref|YP_059708.1| hypothetical protein M6_Spy0390 [Streptococcus pyogenes MGAS10394]
ref|YP_001129023.1| hypothetical protein SpyM51499 [Streptococcus pyogenes str.
Manfredo]
gb|AAT86525.1| Hypothetical protein M6_Spy0390 [Streptococcus pyogenes MGAS10394]
emb|CAM30820.1| putative exported protein [Streptococcus pyogenes str. Manfredo]
Length = 113
Score = 203 bits (517), Expect = 3e-51, Method: Compositional matrix adjust.
Identities = 102/113 (90%), Positives = 108/113 (95%)
Query: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL 60
MTLKKHYYLLSLLALVT GAAFNTSQSVSAQVYSN GY Q LTDEKSHLQYSKDNAQLQL
Sbjct: 1 MTLKKHYYLLSLLALVTFGAAFNTSQSVSAQVYSNGGYRQGLTDEKSHLQYSKDNAQLQL 60
Query: 61 RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
RNIL+GYQ+DLG+HYSSYYYYNLRTVMGLSS+Q IE+HYE+LKNKLHDMYNHY
Sbjct: 61 RNILNGYQDDLGKHYSSYYYYNLRTVMGLSSKQKIEEHYEKLKNKLHDMYNHY 113
>ref|YP_279824.1| hypothetical protein M28_Spy0355 [Streptococcus pyogenes MGAS6180]
gb|AAX71469.1| hypothetical protein M28_Spy0355 [Streptococcus pyogenes MGAS6180]
Length = 113
Score = 201 bits (512), Expect = 9e-51, Method: Compositional matrix adjust.
Identities = 102/113 (90%), Positives = 107/113 (94%)
Query: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL 60
MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSN Y Q LTDEKS LQYSKDNA+LQL
Sbjct: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGRYRQGLTDEKSKLQYSKDNAKLQL 60
Query: 61 RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
RNIL+GYQ+DLGRHYSSYYYYNLRTVMGLS EQ+IEK+YEELKNKLHDMYNHY
Sbjct: 61 RNILNGYQSDLGRHYSSYYYYNLRTVMGLSKEQEIEKYYEELKNKLHDMYNHY 113
>ref|YP_597978.1| hypothetical protein MGAS10270_Spy0369 [Streptococcus pyogenes
MGAS10270]
gb|ABF33434.1| hypothetical protein MGAS10270_Spy0369 [Streptococcus pyogenes
MGAS10270]
Length = 113
Score = 194 bits (492), Expect = 2e-48, Method: Compositional matrix adjust.
Identities = 97/113 (85%), Positives = 108/113 (95%)
Query: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL 60
MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSN GY Q LTD+KSHLQYSKDNA+LQL
Sbjct: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGGYRQDLTDQKSHLQYSKDNAKLQL 60
Query: 61 RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
RNIL+GYQ+DLG+HYSSYYYYNLRTVMGLSS+Q IE+HYE+LK++L+ MYNHY
Sbjct: 61 RNILNGYQSDLGKHYSSYYYYNLRTVMGLSSKQKIEEHYEKLKDELYRMYNHY 113
>ref|NP_664120.1| hypothetical protein SpyM3_0316 [Streptococcus pyogenes MGAS315]
ref|NP_802803.1| hypothetical protein SPs1541 [Streptococcus pyogenes SSI-1]
gb|AAM78923.1| hypothetical protein SpyM3_0316 [Streptococcus pyogenes MGAS315]
dbj|BAC64636.1| hypothetical protein [Streptococcus pyogenes SSI-1]
Length = 113
Score = 179 bits (453), Expect = 7e-44, Method: Compositional matrix adjust.
Identities = 98/113 (86%), Positives = 110/113 (97%)
Query: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL 60
MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYS++GY Q LTD+KSHLQYSKDNA+LQL
Sbjct: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSDKGYRQDLTDQKSHLQYSKDNAKLQL 60
Query: 61 RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
RNIL+GYQ+DLGR+YSSYYYYNLRTVMGLSSEQDIEKHY++LK++L+ MYNHY
Sbjct: 61 RNILNGYQDDLGRNYSSYYYYNLRTVMGLSSEQDIEKHYKKLKDELYRMYNHY 113
>ref|YP_601862.1| hypothetical protein MGAS10750_Spy0368 [Streptococcus pyogenes
MGAS10750]
gb|ABF37318.1| hypothetical protein MGAS10750_Spy0368 [Streptococcus pyogenes
MGAS10750]
Length = 113
Score = 177 bits (448), Expect = 3e-43, Method: Compositional matrix adjust.
Identities = 98/113 (86%), Positives = 107/113 (94%)
Query: 1 MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQL 60
MT+KKHYYLLSLLALVTVGAAFNTSQSVSAQVYSN GY Q LTDEKSHLQYSKDNAQLQL
Sbjct: 1 MTIKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGGYRQGLTDEKSHLQYSKDNAQLQL 60
Query: 61 RNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
RNIL+GYQ+DLGR+YSSYYYYNLRTVMGLS EQ IE+HYE+LK++L+ MYNHY
Sbjct: 61 RNILNGYQDDLGRNYSSYYYYNLRTVMGLSKEQKIEEHYEKLKDELYRMYNHY 113
>ref|NP_606714.1| hypothetical protein spyM18_0491 [Streptococcus pyogenes MGAS8232]
gb|AAL97213.1| hypothetical protein [Streptococcus pyogenes MGAS8232]
Length = 113
Score = 155 bits (392), Expect = 9e-37, Method: Compositional matrix adjust.
Identities = 85/98 (86%), Positives = 95/98 (96%)
Query: 16 VTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQLRNILDGYQNDLGRHY 75
VTVGAAFNTSQSVSAQVYSNEGYHQHLT KSHLQYSKDNAQLQLRNIL+GYQ+DLGR+Y
Sbjct: 16 VTVGAAFNTSQSVSAQVYSNEGYHQHLTGPKSHLQYSKDNAQLQLRNILNGYQSDLGRNY 75
Query: 76 SSYYYYNLRTVMGLSSEQDIEKHYEELKNKLHDMYNHY 113
SSYYYYNLRTVMGLSS+Q+IE+HYE+LK++L+ MYNHY
Sbjct: 76 SSYYYYNLRTVMGLSSKQEIEEHYEKLKDELYRMYNHY 113
>ref|YP_596101.1| hypothetical protein MGAS9429_Spy0369 [Streptococcus pyogenes
MGAS9429]
gb|ABF31557.1| hypothetical protein MGAS9429_Spy0369 [Streptococcus pyogenes
MGAS9429]
Length = 72
Score = 129 bits (324), Expect = 6e-29, Method: Compositional matrix adjust.
Identities = 62/72 (86%), Positives = 69/72 (95%)
Query: 42 LTDEKSHLQYSKDNAQLQLRNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEE 101
+T KSHLQYSKDN +LQLRNIL+GYQ+DLGRHYSSYYYYNLRTVMGLSSEQ+IE+HYE+
Sbjct: 1 MTGPKSHLQYSKDNTKLQLRNILNGYQSDLGRHYSSYYYYNLRTVMGLSSEQEIEEHYEK 60
Query: 102 LKNKLHDMYNHY 113
LKNKLHDMYNHY
Sbjct: 61 LKNKLHDMYNHY 72
>ref|YP_599981.1| hypothetical protein MGAS2096_Spy0385 [Streptococcus pyogenes
MGAS2096]
gb|ABF35437.1| hypothetical protein MGAS2096_Spy0385 [Streptococcus pyogenes
MGAS2096]
Length = 72
Score = 127 bits (320), Expect = 2e-28, Method: Compositional matrix adjust.
Identities = 61/72 (84%), Positives = 69/72 (95%)
Query: 42 LTDEKSHLQYSKDNAQLQLRNILDGYQNDLGRHYSSYYYYNLRTVMGLSSEQDIEKHYEE 101
+T KSHLQYSKDN +LQL+NIL+GYQ+DLGRHYSSYYYYNLRTVMGLSSEQ+IE+HYE+
Sbjct: 1 MTGPKSHLQYSKDNTKLQLKNILNGYQSDLGRHYSSYYYYNLRTVMGLSSEQEIEEHYEK 60
Query: 102 LKNKLHDMYNHY 113
LKNKLHDMYNHY
Sbjct: 61 LKNKLHDMYNHY 72
>ref|YP_599373.1| hypothetical protein MGAS10270_Spy1764 [Streptococcus pyogenes
MGAS10270]
gb|ABF34829.1| hypothetical protein MGAS10270_Spy1764 [Streptococcus pyogenes
MGAS10270]
Length = 106
Score = 36.2 bits (82), Expect = 0.73, Method: Compositional matrix adjust.
Identities = 28/75 (37%), Positives = 40/75 (53%), Gaps = 4/75 (5%)
Query: 5 KHYYLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQLRNIL 64
K LLS AL+ A ++ SVSA Y Q+ T L+ +K+ A+LQ+RN+L
Sbjct: 2 KKTLLLSAAALMVASTAALSTNSVSAYSYDRPLLRQNGTQ---VLREAKNAAELQIRNLL 58
Query: 65 DGYQNDLGRHYSSYY 79
+ Y D G YS Y+
Sbjct: 59 NQYHVD-GEEYSGYF 72
>ref|XP_001308072.1| conserved hypothetical protein [Trichomonas vaginalis G3]
gb|EAX95142.1| conserved hypothetical protein [Trichomonas vaginalis G3]
Length = 934
Score = 35.4 bits (80), Expect = 1.3, Method: Compositional matrix adjust.
Identities = 17/71 (23%), Positives = 38/71 (53%)
Query: 9 LLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQLRNILDGYQ 68
L SL +++V A F + + VSA + N ++ ++++ ++ +L++ ILD +
Sbjct: 70 LKSLPPIISVAAFFGSVECVSALIALNANVYEKDNEKRTPSHFAAAGGKLEIIRILDQDE 129
Query: 69 NDLGRHYSSYY 79
D ++Y +Y
Sbjct: 130 PDTNKNYPIHY 140
>gb|AAM37977.1| chemotaxis protein [Xanthomonas axonopodis pv. citri str. 306]
Length = 663
Score = 33.5 bits (75), Expect = 4.0, Method: Composition-based stats.
Identities = 19/57 (33%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Query: 21 AFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQLRNILDGYQNDLGRHYSS 77
A T+++++A N+ H TDE L + D QLQLRN++ Q D+ +H+ +
Sbjct: 164 ATRTAEAIAAGDLRND-VHTTATDETGRLLQAMDKMQLQLRNLI-AAQTDMAKHHDN 218
>ref|NP_643441.2| chemotaxis protein [Xanthomonas axonopodis pv. citri str. 306]
Length = 722
Score = 33.5 bits (75), Expect = 4.1, Method: Composition-based stats.
Identities = 19/57 (33%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Query: 21 AFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQLRNILDGYQNDLGRHYSS 77
A T+++++A N+ H TDE L + D QLQLRN++ Q D+ +H+ +
Sbjct: 223 ATRTAEAIAAGDLRND-VHTTATDETGRLLQAMDKMQLQLRNLI-AAQTDMAKHHDN 277
>ref|YP_364992.1| methyl-accepting chemotaxis protein [Xanthomonas campestris pv.
vesicatoria str. 85-10]
emb|CAJ24992.1| methyl-accepting chemotaxis protein [Xanthomonas campestris pv.
vesicatoria str. 85-10]
Length = 722
Score = 33.5 bits (75), Expect = 4.1, Method: Composition-based stats.
Identities = 20/70 (28%), Positives = 37/70 (52%), Gaps = 2/70 (2%)
Query: 8 YLLSLLALVTVGAAFNTSQSVSAQVYSNEGYHQHLTDEKSHLQYSKDNAQLQLRNILDGY 67
+L++ + A T+++++A N+ H TDE L + D QLQLRN++
Sbjct: 210 WLITRSLTTPLSQATRTAEAIAAGNLRND-VHTTATDETGRLLQAMDKMQLQLRNLI-AA 267
Query: 68 QNDLGRHYSS 77
Q D+ +H+ +
Sbjct: 268 QTDMAKHHDN 277
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
Posted date: May 10, 2008 4:54 AM
Number of letters in database: 2,222,278,849
Number of sequences in database: 6,515,104
Lambda K H
0.315 0.130 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 6515104
Number of Hits to DB: 480,068,216
Number of extensions: 17875660
Number of successful extensions: 64403
Number of sequences better than 10.0: 58
Number of HSP's gapped: 65762
Number of HSP's successfully gapped: 58
Length of query: 113
Length of database: 2,222,278,849
Length adjustment: 81
Effective length of query: 32
Effective length of database: 1,694,555,425
Effective search space: 54225773600
Effective search space used: 54225773600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 72 (32.3 bits)