Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 53886

HXB2 Location	p17(33-41)	p17 Epitope Map
Author Location	p17(33-41)
Epitope	`HLVWASREL`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(Cw*0602)
Immunogen	HIV-1 infection
Country	India
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, computational epitope prediction, immunodominance

Notes

PBMCs from HIV-1 infected Indian subjects were tested for HIV-1 Gag (with C clade peptides), Nef (with B clade peptides), and Env (clade unspecified) specific T-cell responses. 5 immunodominant regions conserved across clades were identified in Gag and Nef. 3 antigenic regions were found to be recognized by CTLs in the Indian subjects; these regions were not identified as immunodominant in studies from Africa.
26 epitopes were predicted within reactive peptides, of which 90% were clustered in the conserved immunodominant regions of Gag and Nef.
3 of the epitopes were highly conserved across clades, and 7 novel epitopes were found.
This is a novel predicted epitope with >80% conservation to subtype A.

References

Thakar2005 Madhuri R. Thakar, Leena S. Bhonge, Samir K. Lakhashe, U. Shankarkumar, Suvarna S. Sane, Smita S. Kulkarni, Bharati A. Mahajan, and Ramesh S. Paranjape. Cytolytic T Lymphocytes (CTLs) from HIV-1 Subtype C-Infected Indian Patients Recognize CTL Epitopes From a Conserved Immunodominant Region of HIV-1 Gag and Nef. J. Infect. Dis., 192(5):749-759, 1 Sep 2005. PubMed ID: 16088824. Show all entries for this paper.

Displaying record number 53059

HXB2 Location	p17(33-41)	p17 Epitope Map
Author Location	p17
Epitope	`HLVWASREL`	Epitope Alignment
Epitope Name	HL-9
Subtype	C
Species (MHC/HLA)	human(Cw*0804)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD4 T-cell Elispot - IFNγ, CD8 T-cell Elispot - IFNγ, Chromium-release assay, Intracellular cytokine staining
Keywords	subtype comparisons, epitope processing, immunodominance, cross-presentation by different HLA

Notes

Highly targeted regions in Gag for CD8+ T-cells were defined for individuals with C clade infections in South Africa. 9 specific epitopes within the most reactive regions were characterized. This is 1 of 5 novel epitopes that were found among subtype C HIV-1 from African patients who hadn't previously been identified in B clade infections. Some epitopes were shown to be promiscuous, presented by multiple class I restricting alleles.
HLVWASREL was presented by Cw*08 and newly identified in this study; Cw*08 is slightly more common in Zulus than Caucasians (0.066 versus 0.038).

References

Masemola2004a Agatha M. Masemola, Tumelo N. Mashishi, Greg Khoury, Helba Bredell, Maria Paximadis, Tiyani Mathebula, Debra Barkhan, Adrian Puren, Efthyia Vardas, Mark Colvin, Lynn Zijenah, David Katzenstein, Rosemary Musonda, Susan Allen, Newton Kumwenda, Taha Taha, Glenda Gray, James McIntyre, Salim Abdool Karim, Haynes W. Sheppard, and Clive M. Gray. Novel and Promiscuous CTL Epitopes in Conserved Regions of Gag Targeted by Individuals with Early Subtype C HIV Type 1 Infection from Southern Africa. J. Immunol., 173(7):4607-4617, 1 Oct 2004. PubMed ID: 15383595. Show all entries for this paper.

Displaying record number 54142

HXB2 Location	p17(33-41)	p17 Epitope Map
Author Location
Epitope	`HLVWASREL`	Epitope Alignment
Species (MHC/HLA)	human(Cw*0804)
Immunogen
Experimental methods
Keywords	optimal epitope

Notes

C. Brander notes that this is an Cw*0804 epitope.

References

Frahm2008 Nicole Frahm, Brett Baker, and Christian Brander. Identification and Optimal Definition of HIV-Derived Cytotoxic T Lymphocyte (CTL) Epitopes for the Study of CTL Escape, Functional Avidity and Viral Evolution. In Bette Korber, Christian Brander, Barton F. Haynes, Richard Koup, John P. Moore, Bruce D. Walker, and David I. Watkins, editors, HIV Molecular Immunology 2008. page 3 ff. Los Alamos National Laboratory, Theoretical Biology and Biophysics, Los Alamos, New Mexico, 2008. URL: http://www.hiv.lanl.gov/content/immunology. Show all entries for this paper.