Record 341 of 382 - CRIS/ICAR 9/96 TI: DETECTION, EPIDEMIOLOGY, AND ELIMINATION OF VIRUS DISEASES OF SMALL FRUIT CROPS IN: CONVERSE-R-H PO: BOTANY AND PLANT PATHOLOGY. OREGON STATE UNIV LO: CORVALLIS, OREGON 97331. FY: 1993 CL: PRIMARY CLASSIFICATION GENERAL CLASSIF. RPA ACTVTY CMMDTY SCNCE PRCNT PRGM JTC R304 A5000 C1000 F0512 50% P3.13 J2A R205 A4600 C1000 F1112 50% P3.13 J2B PH: R304 (Biological-Efficiency-Fruit,-Vegetables); R205 (Disease-Control-Fruit,-Vegetables); A5000 (Biological-Efficiency-of-Plants,-Animals); A4600 (Protection-Against-Diseases,-Parasites); C1000 (Deciduous-and-Small-Fruits-and-Nuts); F0512 (Genetics-and-Breeding-Plant); F1112 (Pathology-Plant) GH: P3.13 (Fruit); J2A (Plant-Production); J2B (Plant-Protection) SC: S1030 (Berries-and-Cane-Fruits,-General) 25%; S1033 (Strawberries) 25%; S1034 (Raspberries) 25%; S1039 (Other-Berries-and-Cane-Fruits) 25%; PST1 (Target-I-Biology) 50%; PST2 (Target-II-Nonpesticidal-Control) 50%; IPMB (Integrated-Control-Basic-Research) 50% DE: CRINKLE-VIRUS; MILD-YELLOW-EDGE-VIRUS; PALLIDOSIS-AGENT; VEINBANDING-VIRUS; ANTISERA; DISEASE-CONTROL; DISEASE-DETECTION; DISEASE-DIAGNOSIS; MONOCLONAL-ANTIBODIES; MOTTLE; PLANT-DISEASES; PLANT-PATHOLOGY; PLANT-VIRUSES; POLYCLONAL-ANTIBODIES; SEROLOGY; SMALL-FRUIT; STRAWBERRIES; VIRUSES; VIRUS-DETECTION; VIRUS-DISEASES-(PLANTS) CT: Basic 50%; Applied 50%; Developmental 0% OB: Devise and implement rapid biochemical methods for detection of economically important small fruit viruses in the Pacific Northwest. Apply these methods to questions of survival and spread of these viruses and to their elimination in superior small fruit cultivar clones. AP: Develop antisera and rapid serological detection methods for small fruit virusesof economic importance in the Pacific Northwest, emphasizing strawberry viruses, listed in decreasing priority order: mild yellow-edge, crinkle, mottle, pallidosis agent, and veinbanding. For each acquire pure isolates; develop virus purification protocols; eliminate final contaminating healthy components by special methods; develop specific polyclonal/monoclonal antisera; use these antisera in suitable serological tests for the most sensitive accurate virus detection achievable; and apply the developed system to field detection of the virus in crop, weed, and vector reservoirs to work out its ecological relationships. PR: 4712 TO 9305 In cooperation with its parallel USDA ARS project, OR 137 emphasized research onthe identification, characterization, epidemiology and control of the major viruses infecting small fruit crops in Oregon. A number of viruses were identified in strawberry, raspberry, blackberry, blueberry and elderberry. Some were partially characterized, and their methods of dissemination determined. Methods of elimination of these viruses from clones of cultivars were refined and systematically used to develop lines of standard cultivars and advanced selections free from known viruses and virus-like diseases. These lines were then routinely increased free from viruses and made available to requesting scientists and Oregon and other State Departments of Agriculture on an ongoing basis that is still continuing. No patents were applied for on this project. PB: 4712 TO 9305 During the last 26 years of this project and jointly in each case with USDA ARS,a total of 159 research papers were published. All published before 1992 are cited in previous CRIS reports. The following publications appeared in 1992. CONVERSE, R. H. 1992. Modern approaches to strawberry virus research. Acta Horticulturae 308:19-30. ELLIS, P. J., R. H. CONVERSE and R. STACE-SMITH. 1992. Viruses of Sambucus canadensis in North America. Acta Horticulturae 308:69-79. ELLIS, P. J. and R. H. CONVERSE. 1992 Isolation and some properties of a North American carlavirus in Sambucus racemosa. Acta Horticulturae 308:113-120. HADIDI, A., M. S. MONTASSER, L. LEVY, R. W. GOTH, R. H. CONVERSE, M. A. MADKOUR and L. J. SKRZECKOWSKI. 1993. Detection of potato leafroll and strawberry mild yellow-edge luteviruses by reverse transcription-polymerase chain reaction ampl.SPIEGEL, S., E. A. FRISON and R. H. CONVERSE. 1993. Recent developments in therapy and virus-detection procedures for international movement of clonal plant germ plasm. Plant Disease 77:1176-1179. PB: 9201 TO 9212 No publications reported this period. PB: 9101 TO 9112 CONVERSE, R.H. 1992. Modern approaches to strawberry virus research. Acta Horticulturae (In Press). ELLIS, M.A., CONVERSE, R.H., WILLIAMS, R.N. and WILLIAMSON, B. eds. 1991. Compendium of raspberry and blackberry diseases and insects. American Phytopathological Society Press, St. Paul. 100 pp. ELLIS, P.J., CONVERSE, R.H. and STACE-SMITH, R. 1992. Viruses of Sambucus canadensis in North America. Acta Horticulturae (In Press). ELLIS, P.J., STACE-SMITH, R. and CONVERSE, R.H. 1992. Isolation and some properties of a North American carlavirus in Sambucus racemosa. Acta Horticulturae (In Press). YOSHIKAWA, N. and CONVERSE, R.H. 1991. Purification and some properties of strawberry mottle virus. Annals of Applied Biology 118:565-576. PB: 8901 TO 8912 CONVERSE, R.H. 1989. Virus survey of Oregon commercial red raspberry fruiting fields-preliminary results. Proceedings Oregon Horticultural Society 80:143-145. CONVERSE, R.H. 1990. Virus and virus-like diseases of strawberry. HortScience(In press). CONVERSE, R.H. and MARTIN, R.R. 1990. ELISA methods for plant viruses. In R. O. Hampton, ed., Serological methods for detection and identification of viral and bacterial plant pathogens. APS Press, St. Paul, MN (In press). HEPP, R.F. and CONVERSE, R.H. 1990. Aphid transmission of strawberry mottle virus from Chenopodium quinoa to Fragaria vesca. Plant Disease (In press). YOSHIKAWA, N. and CONVERSE, R.H. 1990. Strawberry pallidosis disease: distinctive dsRNA species associated with latent infections in indicators and indiseased strawberry cultivars. Phytopathology (In press). PB: 8801 TO 8812 CONVERSE, R. H. (1988). Status of virus-tested Rubus cultivars in the USDA Research Collection. Fruit Varieties Journal 42:14-19. CONVERSE, R. H. (1988). Tobacco streak and raspberry bushy dwarf viruses in California Boysen fields. Plant Disease 72:175. CONVERSE, R. H., ADAMS, A. N., BARBARA, D. J., CLARK, M. F. and others. (1988). Laboratory detection of viruses and mycoplasmalike organisms in strawberry. Plant Disease 72:744-749. CONVERSE, R. H. and ALINIAZEE, M. T. (1988). Effects of insecticides applied in the field on the incidence of aphid-borne viruses in cultivated strawberry. Plant Disease 72:127-129. CONVERSE, R. H. and SCHAPER, U. (1988). Irregular occurrence of rhabdoviruslike particles in tissues of Fragaria vesca infected with strawberry crinkle virus. J. Phytopathology (in press). GEORGE, R. A. and CONVERSE, R. H. (1988). Methods for the enrichment of desired B-cell populations prior to anti-cauliflower mosaic virus hybridoma formation. Phytopathology 78:1631-1636. HEPP, R. F. and CONVERSE, R. H. (1988). Blueberry red ringspot virus is serologically related to cauliflower mosaic virus. Acta Hort. 236:97-102. PB: 8701 TO 8712 CONVERSE, R.H. 1986. Sterility disorder of "Darrow" blackbberry. HortScience 21:1441-1443. CONVERSE, R.H. and GEORGE, R.A. 1987. Elimination of mycoplasma(s) in blueberry with high carbon dioxide thermotherapy. Plant Disease 71:36-38. HEPP, H. and CONVERSE, R.H. 1987. Blueberry red ringspot virus detection in curde sap of blueberry. Plant Disease 71:536-539. CONVERSE, R.H. 198 . Status of virus-tested Rubus cultivars in the USDA Research Collection. Fruit. Varieties J. (in press). CONVERSE, R.H. 198 . Tobacco streak and raspberry bushy dwarf viruses in California Boysen fields. Plant Disease (in press). CONVERSE, R.H. and ALINIAZEE, M.T. 198 . Effects of insecticides on aphid-borne viruses in strawberry. Plant Disease (in press). CONVERSE, R.H. and SCHAPER, U. 198 . Irregular occurrence of rhabdoviruslike particles of Fragaria vesca plants infected with strawberry crinkle virus. Phytopathology (in press). PB: 8606 TO 8612 CONVERSE, R.H. 1984. New developments in heat therapy to eliminate viruses in Rubus cultivars. Proc. 74th Ann. Mtg Western Washington Horticultural Assn., pp.153-154. SPIEGEL, S. and CONVERSE, R.H. 1985. Detection of strawberry mild yellow-edge virus by serologically specific electron microscopy. Acta Horticulturae 186:95.CONVERSE, R.H. 1985. Latent viruses--harmful or harmless HortScience 20:845-848. MARTIN, R.R. and CONVERSE, R.H. 1985. Purification, some properties and serology of strawberry mild yellow-edge virus. Phytopathologische Zeitschrift 114:21-30. CONVERSE, R.H. 1986. Rates and patterns of tobacco streak virus spread in Boysen and in red raspberry. Acta Horticulturae 186:31-38. CONVERSE, R.H. 1986. The susceptibility of U.S. strawberry cultivars to tomatoringspot virus. Plant Disease 70:369-371. FLORANCE, E.R., ALLEN, T.C. and CONVERSE, R.H. 1986. The ultrastructure of strawberry mild yellow-edge virus, a luteovirus, in cells of Fragaria vesca cultivar UC-4. Phytopathology(Pac Div. 6/24/86, p. 18). (Abstract). PB: 8501 TO 8512 CONVERSE, R.H. 1984. Natural spread of tobacco streak virus in red raspberry. Phytopathology 74:1137. (Abstract). CONVERSE, R.H. 1985. Rates and patterns of tobacco streak virus spread in Boysen and in red raspberry. Acta Horticulture. (In press). CONVERSE, R.H. 1986. The susceptibility of U.S. strawberry cultivars to tomatoringspot virus. Plant Disease 70: (in press). SCHAPER, U. and CONVERSE, R.H. 1985. Detection of mycoplasmalike organisms in infected blueberry cultivars by the DAPI technique. Plant Disease 69:193-196. SPIEGEL, S., COHEN, J. and CONVERSE, R.H. 1985. Detection of strawberry mild yellow-edge virus by serologically specific electron microscopy. Acta Horticulturae. (In press). PB: 8401 TO 8412 CONVERSE, R.H. and TANNE, E. 1984. Heat therapy and shoot apex culture to eliminate mild yellow-edge virus from Hood strawberry. Phytopathology 74:1315-1316. CONVERSE, R.H. and AUGER, J. 1984. Tomato ringspot and raspberry bushy dwarf viruses in red raspberry in Chile (in Spanish). Fitopatologia 19:22-16. CONVERSE, R.H. 1984. Blackberry viruses in the United States. HortScience 19:185-188. CONVERSE, R.H. 1985. Latent viruses: harmful or harmless: HortScience (in press). MARTIN, R.R. and CONVERSE, R.H. 1985. Purification, some properties, and serology of strawberry mild yellow-edge virus. Phytopathologische Zeitschrift 66 (in press). PB: 8301 TO 8312 CONVERSE, R.H. 1983. Reactions of some U.S. strawberry cultivars to graft inoculation with tomato ringspot virus. Phytopathology 73:957 (Abstract). CONVERSE, R.H. 1983. The occurrence of a virus in some indexed strawberry clones. Proc. Oregon Horticultural Society 74:148-150. CONVERSE, R.H. 1983. Virus-tested blueberry cultivars are on the way. Proc. North American Blueberry Council 18:72-77. CONVERSE, R.H. 1983. Recent advances in diagnosis of virus diseases of fruit crops. 4th International Congress of Plant Pathology, Melbourne, Australia (Abstract), p. 25 (No. 100). JOHNSON, H.A., CONVERSE, R.H., ESPEJO, J.I., AMARAO, A., and FRAZIER, N.W. 1984. Seed transmission of tobacco streak virus in strawberry. Plant Disease 68: (in press). PB: 8201 TO 8212 CONVERSE, R.H. 1982. Survey of blueberry virus diseases in Oregon. Proc. Oregon Hort. Society 73:152-153. PB: 8101 TO 8112 CONVERSE, R.H. 1981. A Proposed List of Common Names for Diseases of Caneberry. Plant Disease 65:284-285. CONVERSE, R.H. 1981. An Overview of the 1980 International Rubus Symposium. Proc. Oregon Hort. Soc. 94:100-102. CONVERSE, R.H. 1981. Tomato Ringspot Virus in Naturally Infected, Cultivated Strawberry. pp. 243-244. In: The Strawberry, Cultivars to Marketing, Childers, N.F., Ed. Hort. Publications, Gainesville, Fl. 514 pp. LAWRENCE, F.J., BARRITT, B.H., BRINGHURST, R. and CONVERSE, R.H. 1982. Commercial Strawberry Growing in the Pacific Coast States. USDA Farmers Bulletin No. 2236, Revised and Submitted October, 1981. PB: 8001 TO 8012 CONVERSE, R.H. 1980. Tomato Ringspot Virus in Naturally Infected Cultivated Strawberry. Proc. Oregon Hort. Soc. 71:82-83. FRAZIER, N.W. and CONVERSE, R.H. 1980. Strawberry Vein Banding Virus CML/AAB. Descriptions of Plant Viruses. No. 219. CONVERSE, R.H. 1980. Transmission of Tobacco Streak Virus in Rubus. Acta Hort. 95:53-61. CONVERSE, R.H. and LAWRENCE, F.J. 1980. (Reviewers). Controlling Diseases of Raspberries and Blackberries. USDA FM 2209 Rev. SEP 1980. PB: 7901 TO 7912 CONVERSE, R.H. 1979. Recommended virus indexing procedures for new USDA small fruit and grape cultivars. Plant Dis. Reptr. 63:848-851. CONVERSE, R.H. and BARTLETT, A.B. 1979. Occurrence of viruses in some wild Rubusand Rosa species in Oregon. Plant Dis. Reptr. 63:441-444. CONVERSE, R.H. 1979. Book Review. "Symptoms of virus diseases in plants" by L. Bos. Hort. Science 14:558. CLARKE, R.G., CONVERSE, R.H. and KOJIMA, M. 1980. Enzyme-linked immunosorbent assay to detect potato leafroll virus in potato tubers and viruliferous aphids. Plant Disease 64:43-45. PB: 7801 TO 7812 CONVERSE, R.H. 1978. Detection of tomato ringspot virus in red raspberry by enzyme-linked immunosorant assay (ELISA). Plant Disease Reptr. 62:189-192. CONVERSE, R.H. 1978. Strawberry virus problem. Proc. Western Wash. Hort. Assn. 68:144-145. CONVERSE, R.H. 1978. Pacific Northwest strawberry virus situation. Ore. Hort. Soc. Ann. Rept. 69:78-80. PB: 7701 TO 7712 CONVERSE, R.H. 1976. Serological detection of viruses of Rubus sap. Acta Horticulturae 66:53-61. CONVERSE, R.H. and CASPER, R. 1977. Raspberry bushy dwarf virus in cultivated red raspberry in Germany. Phytopath. Zeitschr. 89:187-190. CONVERSE, R.H. 1977. Certified small fruit stocks. Ore. Hort. Soc. Ann. Rept. 68:60. PB: 7601 TO 7612 CONVERSE, R. H. 1976. Knud George Swenson, 1923-1975. Pytopathology 66:381. CONVERSE, R. H. 1976. Serological detection of viruses in Rubus sap. First Internat. Symposium on Small Fruit Virus Diseases. Heidelberg, Germany. 1 Sept., 1976, p. 7. 9(Abstr.). CONVERSE, R. H. and MARTIN, L. W. 1976. Effects of viruses in strawberry production. W. Wash. Hort. Assn. 66:149-150. PB: 7501 TO 7512 CONVERSE, R. H. 1975. Virus-tested caneberry stock in the Pacific Northwest: supply, usage, findings. Oregon Hort. Soc. Proc. 66:114-116. CONVERSE, R. H. and R. CASPER. 1975. A member of the apple mosaic virus group occurring in Rubus. Acta Horticulturae 44:63. PB: 7401 TO 7412 CONVERSE, R.H. The propagation of virus-tested strawberry stocks. Jour. International Plant Propagation Soc. 22 (1973):73-77. CONVERSE, R.H. Strawberry and red raspberry planting trends in the Pacific Northwest Proc. Western Washington Horticultural Assoc. 64 (1974):92-96. CONVERSE, R.H. Thermotherapy of viruses in Santiam blackberry. Phytopathology Abstracts 66th Ann. Mtg. (1974): 117-118. CONVERSE, R.H. and L.W. MARTIN. Strawberry cultivars compared for virus content, runner and fruit production. J. Amer. Soc. Horticultural Science 99 (1974):163-166. PB: 7301 TO 7312 CONVERSE, R.H. and A.D. DAVISON. Viruses in red raspberry in Western Washington. Plant Disease Reporter. 56(1972):1007-1009. LISTER, R.M. and R.H. CONVERSE. Black raspberry latent virus. CMI/AB Descriptions of Plant Viruses. No. 106 (1972) 4p. CONVERSE, R.H. Occurrence and some prperties of raspberry bushy dwarf virus in Rubus species in the United States. Phytopathology 63(1973):780-783. CONVERSE, R.H. Tomato ringspot virus and Phytopathora root rot in relation to decline disease in red raspberry. Proceedings, Western Washington Horticultural Association. 63(1973):156-159. PB: 7201 TO 7212 CONVERSE, R. H. 1971. Identification and control of tomato ringspot virus in red raspberry. Proceedings, Western Washington Horticultural Association 61:74,76. CONVERSE, R. H. 1972. Tobacco streak virus in black raspberry. Phytopathology 62:1001-1004. CONVERSE, R. H. 1972. Occurrence of raspberry bushy dwarf virus in Rubus species in the United States. Phytopathology 62:752 (Abstract). PB: 7101 TO 7112 CONVERSE, R.H. and A.B. BARTLETT. Occurrence of strawberry lethal decline disease in Oregon. Plant Disease Reporter 55:560. 1971. CONVERSE, R.H., H.A. DAUBENY, R. STACE-SMITH, L.M. RUSSELL, E.J. KOCH and S.C. WIGGANS. Search for biological races in Amphorphora agathonica Hottes on red raspberries. Canadian Journal of Plant Science 51:81-85. 1971. CONVERSE, R.H. and R. STACE-SMITH. Rate of spread and effect of tomato ringspotvirus on red raspberry in the field. Phytopathology 61:1104-1106. 1971. HUHTANEN, D.P. and R.H. CONVERSE. Temporary symptom remission of strawberry June yellows and witches'-broom by use of oxytetracycline. Phytopathology 61:1137-1139. 1971. MARTIN, L.W. and R.H. CONVERSE. Studies on strawberry plant production in Oregon in 1970. Plant Disease Reporter 55:220-222. 1971. PB: 7001 TO 7012 CONVERSE,R.H. Effect of oil sprays on spread of strawberry viruses. Plant Disease Reporter 54 (1970): 479-482. CONVERSE,R.H. Occurrence of Phytophthora fragariae race A-10 in California. Plant Disease Reporter 54 (1970): 969-971. CONVERSE,R.H. The occurrence, detection, and control of Rubus viruses. In N. W. Frazier (ed.) Virus Diseases of Small Fruits and Grapevines. Division of Ag. Sciences, U. of Calif. Berkeley. (1970): pp 107-110. CONVERSE,R.H. and L.W.MARTIN. A comparison of strawberry nursery sites in three areas in Oregon. Oregon Horticultural Society Proceedings 61 (1970): 101-103. CONVERSE,R.H., R.STACE-SMITH, and F.JOHNSON. Virus diseases observed on cultivated red raspberries in the Pacific Northwest. Plant Disease Reporter 54 (1970): 701-703. PB: 6701 TO 6712 CARLTON, K.L. and VAUGHAN, E.K. Control of Strawberry root diseases. American Physopathological Society Fungicide-Nematocide Tests, Results for 1966., 22 (1966), 115-116. PB: 6801 TO 6812 CONVERSE, R.H. Necrotic shock virus in strawberries in Oregon. Phytopathology 59:(1969) 10. CONVERSE, R.H. Strawberry red stele on the Pacific Coast. Proc. Western Washington Horticultural Association 58:(1968) 80-82. PB: 6901 TO 6912 CONVERSE,R.H. and R.M.LISTER. The occurrence and some properties of black raspberry latent virus. Phytopathology 59:(1969) 325-333. CONVERSE,RH.H. A survey of viruses in cultivated strawberries in Oregon and Washington. Plant Disease Reporter 53:(1969) 610-614. CONVERSE,R.H. Occurrence of necrotic shock virus in strawberries in the Pacific Northwest. Plant Disease Reporter 53: (1969) 608-610. CONVERSE,R.H. and B.Y.ENDO. Occurrence of tomato ringspot virus in red raspberries in the eastern United States. Phytopathology 58: (1969) 216-218. CONVERSE,R.H. The virus status of Oregon strawberries. Ore. Hort. Soc. Proc. (1968)60:106- 108-110. OC: 000550 IC: 003210 SU: Region 4 (Western). PS: TERMINATED UD: 8606 UP: 9511 PP: 9305 --------------------------------------------------------------------------------