Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | Plerocercoid growth factor (PGF), a human growth hormone (hGH) analogue produced by the tapeworm Spirometra mansonoides, has direct insulin-like action in adipose tissue of normal rats in vitro |
| Creator/Author | Salem, M.A.M. ; Phares, C.K. |
| Publication Date | 1986 Mar 01 |
| OSTI Identifier | OSTI ID: 5385152 |
| Report Number(s) | CONF-8604222- |
| Other Number(s) | CODEN: FEPRA |
| Resource Type | Conference |
| Specific Type | Journal Article |
| Resource Relation | Fed. Proc., Fed. Am. Soc. Exp. Biol. ; Vol/Issue: 45:3; 70. annual meeting of the Federation of American Society for Experimental Biology; 13 Apr 1986; St. Louis, MO, USA |
| Research Org | Univ. of Nebraska Medical Center, Omaha |
| Subject | 550201 -- Biochemistry-- Tracer Techniques; BODY TEMPERATURE-- BIOLOGICAL VARIABILITY;TRH-- BIOLOGICAL EFFECTS;TRH-- RECEPTORS; AFFINITY;BIOCHEMICAL REACTION KINETICS;BRAIN;DOSE-RESPONSE RELATIONSHIPS;HYPERTHERMIA;INTRAPERITONEAL INJECTION;MICE;TRACER TECHNIQUES;TRITIUM COMPOUNDS |
| Related Subject | ANIMALS;BODY;BODY TEMPERATURE;CENTRAL NERVOUS SYSTEM;HORMONES;INJECTION;INTAKE;ISOTOPE APPLICATIONS;KINETICS;LABELLED COMPOUNDS;MAMMALS;MEMBRANE PROTEINS;NERVOUS SYSTEM;ORGANIC COMPOUNDS;ORGANS;PEPTIDE HORMONES;PROTEINS;REACTION KINETICS;RODENTS;VERTEBRATES |
| Description/Abstract | The metabolic actions of GH can be divided into acute (insulin-like) and chronic (lipolytic/anti-insulin).^The insulin-like actions of GH are most readily elicited in GH-deficient animals as GH induces resistance to its own insulin-like action.^Like GH, PGF stimulates growth and cross-reacts with anti-hGH antibodies.^Independent experiments were conducted comparing the direct actions of PGF to insulin or hGH in vitro.^Insulin-like effects were determined by the ability of PGF, insulin or hGH to stimulate (U-/sup 14/C)glucose metabolism in epidydimal fat pads from normal rats and by inhibition of epinephrine-stimulated lipolysis.^Direct stimulation of lipolysis was used as anti-insulin activity.^To determine if PGF competes for insulin or GH receptors, adipocytes (3 x 10/sup 5/ cells/ml) were incubated with either (/sup 125/I)insulin or (/sup 125/I)hGH +/- PGF, +/- insulin or +/- hGH.^PGF stimulated glucose oxidation and /sup 14/C-incorporation into lipids.^Insulin, hGH and PGF inhibited lipolysis (33%, 29% and 34%, respectively).^Adipose tissue was very sensitive to the lipolytic effect of hGH but PGF was neither lipolytic nor did it confer refractoriness to its insulin-like action.^PGF bound to GH but not to insulin receptors.^Therefore, PGF had direct insulin-like effects but did not stimulate lipolysis in tissue from normal rats in vitro. |
| Country of Publication | United States |
| Language | English |
| Format | Pages: 406 |
| System Entry Date | 2001 May 13 |
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Last Updated: 02/14/2009