HT0911

Library Description

Organism:	Homo sapiens
Developmental stage:	Adult
Organ:	head_neck
Vector:	puc18
Vector type:	Plasmid
Cloning site 1:	SmaI
Cloning site 2:	SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

643 ESTs from this library were grouped into 291 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
62	96423	Hs.631731	Proline-rich protein HaeIII subfamily 2, mRNA (cDNA clone MGC:168111 IMAGE:9020488).
35	54432	Hs.203717	Migration stimulation factor FN70+ (FN gene).
30	46656	Hs.430733	Chloride ion current inducer protein (ICln).
18	27994	Hs.700779	CDNA FLJ53910 complete cds, highly similar to Keratin, type II cytoskeletal 6A.
15	23328	Hs.433845	CDNA FLJ54081 complete cds, highly similar to Keratin, type II cytoskeletal 5.
10	15552	Hs.408153	LPRP=pHL E1F1 [human, lacrimal gland, mRNA Partial, 507 nt].
9	13997	Hs.713750	Transcribed locus.
9	13997	Hs.673372	Transcribed locus, strongly similar to NP_001129575.1 transmembrane protein 132C [Homo sapiens].
8	12442	Hs.709235	Keratin 6 isoform K6f (KRT6F).
7	10886	Hs.703599	Transcribed locus, strongly similar to XP_001231536.1 PREDICTED: similar to Ac1147 [Gallus gallus].
7	10886	Hs.430551	KIAA0051.
5	7776	Hs.716433	Protein phosphatase 2, regulatory subunit B', alpha isoform, mRNA (cDNA clone MGC:26270 IMAGE:4815091).
5	7776	Hs.714394	Partial mRNA (non-coding TFEBAlpha gene), tumor 1.
5	7776	Hs.642877	Heterogenous nuclear RNA W16W.
5	7776	Hs.516157	S-adenosylmethionine synthetase.
5	7776	Hs.499145	YME1-like 1 (S. cerevisiae), mRNA (cDNA clone MGC:3533 IMAGE:3637576).
5	7776	Hs.494173	Annexin A1, mRNA (cDNA clone MGC:32774 IMAGE:4662939).
5	7776	Hs.472471	Chromatin modifying protein 4B, mRNA (cDNA clone MGC:45387 IMAGE:5173394).
5	7776	Hs.458355	C1S mRNA, transcript variant 3.
5	7776	Hs.437855	Homo sapiens, clone IMAGE:3949956, mRNA.
5	7776	Hs.421608	Elongation factor 1-beta {5' region, clone CEM1002} [human, lymphoblastoid cell line CEM, mRNA Partial, 513 nt].
5	7776	Hs.229641	Transcriptional coactivator PC4.
4	6221	Hs.688468	Transcribed locus.
4	6221	Hs.529609	PolyA site DNA.
4	6221	Hs.518450	CDNA FLJ76709 complete cds, highly similar to Homo sapiens Huntington disease-associated protein (HD) mRNA.
4	6221	Hs.495854	CDNA FLJ32736 fis, clone TESTI2001237, weakly similar to RING CANAL PROTEIN.
4	6221	Hs.477869	Phospholipid scramblase 4 (PLSCR4), transcript variant 3, mRNA.
4	6221	Hs.431043	Homeodomain protein Prep-1.
3	4666	Hs.714435	Transcribed locus, strongly similar to NP_001028196.1 F-box only protein 7 isoform 2 [Homo sapiens].
3	4666	Hs.714318	CDNA FLJ60688 complete cds, highly similar to Replication factor C subunit 4.
3	4666	Hs.713979	UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4), mRNA (cDNA clone MGC:41784 IMAGE:5261236).
3	4666	Hs.709313	Beta 2- mu =beta 2-microglobulin [human, SK-MEL-33 cells, mRNA Mutant, 433 nt].
3	4666	Hs.709234	Keratin 6C (KRT6C), mRNA.
3	4666	Hs.685289	Transcribed locus, strongly similar to NP_001173.1 antiquitin [Homo sapiens].
3	4666	Hs.659981	Transcribed locus.
3	4666	Hs.659767	CDNA FLJ60761 complete cds, highly similar to Hydroxyacid oxidase 2 (EC 1.1.3.15).
3	4666	Hs.654801	KIAA0442.
3	4666	Hs.603766	Mediator complex subunit 13-like, mRNA (cDNA clone MGC:163294 IMAGE:40146453).
3	4666	Hs.595071	Glucosidase II.
3	4666	Hs.594537	Meltrin-S (ADAM12) mRNA, complete cds, alternatively spliced.
3	4666	Hs.546288	Ribosomal protein S9.
3	4666	Hs.524630	MRNA; cDNA DKFZp564A0264 (from clone DKFZp564A0264).
3	4666	Hs.511915	MRNA fragment for neurone-specific enolase.
3	4666	Hs.499000	DnaJ (Hsp40) homolog, subfamily C, member 1, mRNA (cDNA clone IMAGE:4606307).
3	4666	Hs.477440	OSBP related protein 11.
3	4666	Hs.469313	CDNA FLJ33856 fis, clone CTONG2005913, moderately similar to Mus musculus prominin-like protein mRNA.
3	4666	Hs.448588	Unknown mRNAs.
3	4666	Hs.259046	Chromosome 3 open reading frame 55 (C3orf55), transcript variant 2, mRNA.
2	3110	Hs.716374	SMRP.
2	3110	Hs.715686	CAMP-binding guanine nucleotide exchange factor III (cAMP-GEFIII) mRNA, clone 172, partial sequence.
2	3110	Hs.714524	Transcribed locus, strongly similar to NP_666166.2 pleckstrin homology domain containing, family C (with FERM domain) member 1 [Mus musculus].
2	3110	Hs.713649	Transcribed locus, strongly similar to XP_529285.2 PREDICTED: hypothetical protein [Pan troglodytes].
2	3110	Hs.709758	Transcribed locus.
2	3110	Hs.709193	CDNA FLJ38010 fis, clone CTONG2012476, weakly similar to GTP-BINDING PROTEIN TC10.
2	3110	Hs.706025	Transcribed locus.
2	3110	Hs.702907	PPP1CB mRNA.
2	3110	Hs.699306	High molecular weight B cell growth factor mRNA sequence.
2	3110	Hs.699195	SOX-4 protein.
2	3110	Hs.688742	Transcribed locus.
2	3110	Hs.687186	Transcribed locus.
2	3110	Hs.679935	Transcribed locus.
2	3110	Hs.679643	Transcribed locus.
2	3110	Hs.679102	CDNA FLJ44331 fis, clone TRACH3003586.
2	3110	Hs.670587	Transcribed locus.
2	3110	Hs.662937	MRNA; cDNA DKFZp564F133 (from clone DKFZp564F133).
2	3110	Hs.659062	Transcribed locus.
2	3110	Hs.655552	Development and differentiation enhancing factor 1, mRNA (cDNA clone MGC:168755 IMAGE:9021132).
2	3110	Hs.654634	CDNA FLJ37601 fis, clone BRCOC2009196, highly similar to CDC42-binding protein kinase beta (DMPK-like).
2	3110	Hs.652076	CDNA FLJ32814 fis, clone TESTI2002806.
2	3110	Hs.646575	26S proteasome subunit p55.
2	3110	Hs.643801	Versican V2 core protein precursor splice-variant.
2	3110	Hs.643120	Insulin-like growth factor 1 receptor mRNA, 3' sequence.
2	3110	Hs.632339	CEV14.
2	3110	Hs.601739	Transcribed locus.
2	3110	Hs.597924	Transcribed locus.
2	3110	Hs.558296	Cytoplasmic phosphotyrosyl protein phosphatase (clone type 2) 5'end.
2	3110	Hs.557425	Jumonji domain containing 1A, mRNA (cDNA clone IMAGE:4823253).
2	3110	Hs.55304	Krueppel-related DNA-binding protein (TF9 PF4) mRNA, 5' cds.
2	3110	Hs.530331	Pyruvate dehydrogenase alpha subunit {3'region} [human, mRNA Partial Mutant, 129 nt].
2	3110	Hs.518346	CDNA FLJ52061 complete cds, highly similar to Translocon-associated protein subunit gamma.
2	3110	Hs.517293	Homo sapiens, clone IMAGE:3634113, mRNA.
2	3110	Hs.510078	Putative serine/threonine protein kinase.
2	3110	Hs.483239	Antiquitin=26g turgor protein homolog [human, kidney, mRNA, 1809 nt].
2	3110	Hs.479898	Estrogen sulfotransferase.
2	3110	Hs.478275	CDNA FLJ51976 complete cds, moderately similar to Tumor necrosis factor ligand superfamily member 10.
2	3110	Hs.458334	Transcribed locus, strongly similar to NP_001019853.1 hypothetical protein LOC283755 [Homo sapiens].
2	3110	Hs.443139	Hypothetical protein KIAA1833, mRNA (cDNA clone IMAGE:5756917).
2	3110	Hs.440599	CDNA FLJ57326 complete cds, highly similar to ATP-dependent RNA helicase DDX1 (EC 3.6.1.-).
2	3110	Hs.438906	Chromosome 22 open reading frame 30, mRNA (cDNA clone MGC:50372 IMAGE:5535580).
2	3110	Hs.434937	Cyclophilin mRNA, 3' end.
2	3110	Hs.433026	Transcribed locus, strongly similar to XP_508554.2 PREDICTED: similar to ubiquitin-like protein [Pan troglodytes].
2	3110	Hs.390428	MAP kinase kinase kinase (MTK1).
2	3110	Hs.374477	EWS mRNA.
2	3110	Hs.336994	KIAA0429.
2	3110	Hs.279806	P68 protein.
2	3110	Hs.162757	MRNA fragment for alpha-2 macroglobulin receptor.
2	3110	Hs.145442	Homosapiens ERK activator kinase (MEK1) mRNA.
2	3110	Hs.120	Peroxiredoxin 6, mRNA (cDNA clone MGC:46173 IMAGE:5767682).
2	3110	Hs.10784	HBV XAg-transactivated protein 11 splice variant 1 (XTP11) mRNA, complete cds, alternatively spliced.
1	1555	Hs.79136	Solute carrier family 39 (zinc transporter), member 6, mRNA (cDNA clone MGC:50406 IMAGE:5552785).