Water samples were collected from the rosette.  Samples collected from the surface to 250 metes were filtered 
using precombusted (450°C) GF/F inline filters as they were being collected from the Niskin bottle.  At 
depths > 250 meters the samples were collected without filtration.  After collection, samples were frozen 
upright in 60 ml acid-cleaned HDPE bottles, and remained cold until analysis.  Prior to analysis, samples 
were returned to room temperature then acidified to pH < 2 with concentrated hydrochloric acid.  Analysis 
was performed using a Shimadzu TOC-VCSH Total Organic Carbon Analyzer with the TNM-1 Total Nitrogen detector.  
Instrument conditions were as follows:

	Combustion Temperature		680 °C
	Carrier Gas			UHP Oxygen
	Carrier Flow Rate		150 ml/min
	Ozone Generation Gas		Zero Air from Whatman TOC Gas Generator
	Ozone Flow Rate 		500 ml/min
	Sample Sparge time		2.0 minutes
	Minimum number of injections	3
	Maximum number of injections	5
	Number of Washes		2
	Standard Deviation Maximum	0.1000
	CV Maximum			2.00%
	Injection Volume		100 ul

The TOC system was calibrated using potassium hydrogen phthalate in Milli-Q water and the TN system was calibrated 
using potassium nitrate in Milli-Q water.  System performance was verified daily using Consensus Reference Water.  
This reference water is deep Sargasso Seawater that has been acidified and sealed in 10ml ampoules, the concentration 
of which has been determined by the consensus of up to six expert and independent laboratories.  After verifying 
proper operation of the TOC/TN instrument, samples were set up on an auto sampler for analysis.  The run started 
with a QW (Q Water) blank and a reference seawater analysis.  Then six samples would be analyzed followed by another 
QW blank and reference seawater.  This sequence would be repeated until all samples for that run were analyzed.  
The run ended with a QW blank, reference water and a QW blank that had not been acidified.  This was done to verify 
that the hydrochloric acid used to acidify the samples was not contaminated.  QW blanks and reference water samples 
were used to evaluate system performance during the analytical run.  If a problem was detected with the blanks or 
reference waters, the samples were reanalyzed.