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Title Pasteurella haemolytica bacteriophage: identification, partial characterization, and relationship of temperate bacteriophages from isolates of Pasteurella haemolytica (biotype A, serotype 1)
Creator/Author Richards, A.B. ; Renshaw, H.W. ; Sneed, L.W.
Publication Date1985 May 01
OSTI IdentifierOSTI ID: 5027210
Other Number(s)CODEN: AJVRA
Resource TypeJournal Article
Resource RelationAm. J. Vet. Res. ; Vol/Issue: 5
Subject560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987); BACTERIA-- BIOLOGICAL RADIATION EFFECTS; BACTERIOPHAGES;CATTLE;DOSE-RESPONSE RELATIONSHIPS;LUNGS;PLAQUE FORMATION;ULTRAVIOLET RADIATION
Related SubjectANIMALS;BIOLOGICAL EFFECTS;BODY;DOMESTIC ANIMALS;ELECTROMAGNETIC RADIATION;MAMMALS;MICROORGANISMS;ORGANS;PARASITES;RADIATION EFFECTS;RADIATIONS;RESPIRATORY SYSTEM;RUMINANTS;VERTEBRATES;VIRUSES
Description/Abstract Pasteurella haemolytica (biotype A, serotype 1) isolates (n = 15) from the upper respiratory tract of clinically normal cattle, as well as from lung lesions from cases of fatal bovine pasteurellosis, were examined for the presence of bacteriophage after irradiation with UV light.^Treatment of all P haemolytica isolates with UV irradiation resulted in lysis of bacteria due to the induction of vegetative development of bacteriophages.^The extent of growth inhibition and bacterial lysis in irradiated cultures was UV dose-dependent.^Bacterial cultures exposed to UV light for 20 s reached peak culture density between 60 and 70 minutes after irradiation; thereafter, culture density declined rapidly, so that by 120 minutes, it was approximately 60% of the original value.^When examined ultrastructurally, lytic cultures from each isolate revealed bacteriophages with an overall length of approximately 200 nm and that appeared to have a head with icosahedral symmetry and a contractile tail.^Cell-free filtrate from each noninduced bacterial isolate was inoculated onto the other bacterial isolates in a cross-culture sensitivity assay for the presence of phages lytic for the host bacterial isolates.^Zones of lysis (plaques) did not develop when bacterial lawns grown from the different isolates were inoculated with filtrates from the heterologous isolates.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1215-1220
System Entry Date2001 May 13

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