ALYASEEN SA, HERRERO IA, ROUSE MS, PIPER KE, STECKELBERG JM, PATEL R; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. L-767.
Mayo Clinic, Rochester, MN.
BACKGROUND: Organisms historically categorized as S. bovis may represent one of several biotypes of S. bovis, or even non-S. bovis streptococci. It is unknown whether organisms isolated from endocarditis patients and classically described as "S. bovis' associated with carcinoma of the colon represent a unique S. bovis biotype or other recently described streptococci. METHODS: We reviewed the Mayo Clinic endocarditis database (1975 to 1985) to identify S. bovis or streptococcus group D nonenterococcus endocarditis cases. 16S rDNA PCR amplification and bidirectional sequencing of 1430 nucleotides were performed on archived isolates. RESULTS: Fourteen cases were identified. Using 16S rDNA analysis the organisms were identified as S. bovis biotype I (n = 11), S. bovis biotype II/2 (n = 1), S. salivarius (n = 1), and S. macedonicus (n = 1). No organisms representing S. gallolyticus were identified. Two patients (both with S. bovis biotype I endocarditis) were found to have colon cancer and 6 patients [with S. bovis biotype I (n =5) and S. macedonicus (n = 1) endocarditis] had other gastrointestinal pathology (ulcerative colitis, sigmoid colon villous adenoma, cecal polyp, gastric adenomatous polyps, sigmoid colon diverticulosis). Conclusion: This is the largest series of S. bovis endocarditis isolates subjected to near full-length 16S rDNA sequence analysis. This is the first description of human infection with S. macedonicus, an organism originally isolated from Greek cheese. S. bovis biotype I was the predominant organism causing endocarditis in this series and was associated with malignant and pre-malignant colon lesions. It is not known if S. bovis biotype II or other closely related organisms (e.g. S. macedonicus) are associated with malignant and premalignant colon lesions in humans. This has clinical significance in terms of whether or not patients infected with these organisms should be evaluated for colonic pathology.
Publication Types:
Keywords:
- Base Sequence
- DNA, Recombinant
- DNA, Ribosomal
- Endocarditis
- Evaluation Studies
- Humans
- Polymerase Chain Reaction
- Research
- Streptococcus
- Streptococcus bovis
- analysis
- genetics
Other ID:
UI: 102266985
From Meeting Abstracts