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Title Pharmacologic characterization and autoradiographic distribution of binding sites for iodinated tachykinins in the rat central nervous system
Creator/Author Buck, S.H. ; Helke, C.J. ; Burcher, E. ; Shults, C.W. ; O`Donohue, T.L.
Publication Date1986 Nov 01
OSTI IdentifierOSTI ID: 6525289
Other Number(s)CODEN: PPTDD
Resource TypeJournal Article
Resource RelationPeptides (Fayetteville, N.Y.) ; Vol/Issue: 6
Research OrgSection on Biochemical Pharmacology, NHLBI, Bethesda, MD
Subject550201 -- Biochemistry-- Tracer Techniques; KININS-- RECEPTORS;RECEPTORS-- AUTORADIOGRAPHY;RECEPTORS-- BIOCHEMICAL REACTION KINETICS; BRAIN;CELL MEMBRANES;CELL NUCLEI;CENTRAL NERVOUS SYSTEM;COMPARATIVE EVALUATIONS;IODINE 125;PEPTIDES;RATS;TISSUE DISTRIBUTION
Related SubjectANIMALS;BETA DECAY RADIOISOTOPES;BODY;CELL CONSTITUENTS;CENTRAL NERVOUS SYSTEM;DAYS LIVING RADIOISOTOPES;DISTRIBUTION;ELECTRON CAPTURE RADIOISOTOPES;INTERMEDIATE MASS NUCLEI;IODINE ISOTOPES;ISOTOPES;KINETICS;MAMMALS;MEMBRANE PROTEINS;MEMBRANES;NERVOUS SYSTEM;NUCLEI;ODD-EVEN NUCLEI;ORGANIC COMPOUNDS;ORGANS;PEPTIDES;POLYPEPTIDES;PROTEINS;RADIOISOTOPES;REACTION KINETICS;RODENTS;VERTEBRATES
Description/Abstract P-type, E-type, and K-type tachykinin binding sites have been identified in the mammalian CNS.^These sites may be tachykinin receptors for which the mammalian neuropeptides substance P, neuromedin K, and substance K are the preferred natural agonists, respectively.^In the present investigation, we have compared the pharmacology and the autoradiographic distribution of CNS binding sites for the iodinated (/sup 125/I-Bolton-Hunter reagent) tachykinins substance P, eledoisin, neuromedin K, and substance K. Iodinated eledoisin and neuromedin K exhibited an E-type binding pattern in cortical membranes.^Iodinated eledoisin, neuromedin K, and substance K each labeled sites that had a similar distribution but one that was considerably different from that of sites labeled by iodinated substance P. CNS regions where there were detectable densities of binding sites for iodinated eledoisin, neuromedin K, and substance K and few or no sites for iodinated substance P included cortical layers IV-VI, mediolateral septum, supraoptic and paraventricular nuclei, interpeduncular nucleus, ventral tegmental area, and substantia nigra pars compacta.^Binding sites for SP were generally more widespread in the CNS.^CNS regions where there was a substantial density of binding sites for iodinated substance P and few or no sites for iodinated eledoisin, neuromedin K, and substance K included cortical layers I and II, olfactory tubercle, nucleus accumbens, caudate-putamen, globus pallidus, medial and lateral septum, endopiriform nucleus, rostral thalamus, medial and lateral preoptic nuclei, arcuate nucleus, dorsal raphe nucleus, dorsal parabrachial nucleus, parabigeminal nucleus, cerebellum, inferior olive, nucleus ambiguus, retrofacial and reticular nuclei, and spinal cord autonomic and somatic motor nuclei.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1109-1120
System Entry Date2001 May 13

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