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Title Distribution of 125I-galanin binding sites, immunoreactive galanin, and its coexistence with 5-hydroxytryptamine in the cat spinal cord: Biochemical, histochemical, and experimental studies at the light and electron microscopic level
Creator/Author Arvidsson, U. ; Ulfhake, B. ; Cullheim, S. ; Bergstrand, A. ; Theodorson, E. ; Hoekfelt, T. (Karolinska Institutet, Stockholm (Sweden))
Publication Date1991 Jun 01
OSTI IdentifierOSTI ID: 5229670
Other Number(s)ISSN0021-9967; CODEN: JCNEA
Resource TypeJournal Article
Resource RelationJournal of Comparative Neurology ; Vol/Issue: 308:1
Subject550201 -- Biochemistry-- Tracer Techniques; PEPTIDES-- RECEPTORS;RECEPTORS-- TISSUE DISTRIBUTION;SPINAL CORD-- BIOCHEMISTRY; AUTORADIOGRAPHY;BIOCHEMICAL REACTION KINETICS;CATS;IODINE 125;MICROSCOPY;RADIOIMMUNOASSAY;SEROTONIN
Related SubjectAMINES;ANIMALS;AROMATICS;AUTONOMIC NERVOUS SYSTEM AGENTS;AZAARENES;AZOLES;BETA DECAY RADIOISOTOPES;BIOASSAY;CENTRAL NERVOUS SYSTEM;CHEMISTRY;DAYS LIVING RADIOISOTOPES;DIAGNOSTIC TECHNIQUES;DISTRIBUTION;DRUGS;ELECTRON CAPTURE RADIOISOTOPES;HETEROCYCLIC COMPOUNDS;HYDROXY COMPOUNDS;IMMUNOASSAY;IMMUNOLOGY;INDOLES;INTERMEDIATE MASS NUCLEI;INTERNAL CONVERSION RADIOISOTOPES;IODINE ISOTOPES;ISOTOPE APPLICATIONS;ISOTOPES;KINETICS;MAMMALS;MEMBRANE PROTEINS;NERVOUS SYSTEM;NEUROREGULATORS;NUCLEI;ODD-EVEN NUCLEI;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;PROTEINS;PYRROLES;RADIOASSAY;RADIOIMMUNODETECTION;RADIOIMMUNOLOGY;RADIOISOTOPES;RADIOPROTECTIVE SUBSTANCES;REACTION KINETICS;SYMPATHOMIMETICS;TRACER TECHNIQUES;TRYPTAMINES;VERTEBRATES
Description/Abstract The distribution of galanin-like immunoreactivity (GAL-LI) in the spinal cord of the cat was studied by use of indirect histochemistry and the peroxidase-antiperoxidase (PAP) technique.^In the ventral horn GAL-immunoreactive (IR) axonal fibers and terminals were most frequent in the ventral part of the motor nucleus.^The GAL-IR axons also contained 5-hydroxytryptamine (5-HT)-LI, and they disappeared after spinal cord transection.^It was concluded that these GAL-IR fibers belong to the serotoninergic bublospinal pathway.^In the medulla oblongata from normal cats, scattered GAL-IR cell bodies were encountered within the nucleus raphe obscurus and nucleus raphe pallidus.^Electron microscopic observations revealed that the fine structure of the GAL-IR axonal boutons in the motor nucleus was similar to that of 5-HT-IR boutons with a varying number of immunoreactive large dense core vesicles.^The postsynaptic element in all cases studied was a dendrite.^A dense GAL-IR axonal plexus was found in the superficial laminae I-II of the dorsal horn.^Coexistence was found between the GAL- and substance P-LI in fibers within the dorsal horn plexus.^Spinal cord transection did not alter the pattern of GAL-LI in the dorsal horn, while the vast majority of GAL-IR axonal swellings disappeared following dorsal root sectioning.^Electron microscopic observations in lamina II (substantia gelatinosa) revealed that the GAL-IR axonal terminals could be divided into two main groups.^One with small to medium-sized axonal boutons formed synaptic contacts with both dendritic and axonal profiles.^The other formed the central axon terminals of glomeruli, suggesting that GAL-LI may be present in C-type primary afferents.^Numerous small GAL-IR cell bodies were encountered in laminae II and III.^GAL-IR cell bodies were also observed in lamina X.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 115-138
System Entry Date2001 May 13

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