Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | Repair of nonreplicating UV-irradiated DNA |
| Creator/Author | Martin, S.J. ; Hays, J.B. |
| Publication Date | 1986 May 01 |
| OSTI Identifier | OSTI ID: 6998340 |
| Report Number(s) | CONF-8606151- |
| Other Number(s) | CODEN: FEPRA |
| Resource Type | Conference |
| Specific Type | Journal Article |
| Resource Relation | Fed. Proc., Fed. Am. Soc. Exp. Biol. ; Vol/Issue: 45:6; 76. annual meeting of the Federation of American Society for Experimental Biology; 8 Jun 1986; Washington, DC, USA |
| Research Org | Univ. of Maryland Baltimore County, Catonsville |
| Subject | 560130 -- Radiation Effects on Microorganisms; BACTERIOPHAGES-- IRRADIATION;DNA-- GENETIC RADIATION EFFECTS;ESCHERICHIA COLI-- DNA REPAIR; FROGS;GENES;OOCYTES;TRANSCRIPTION;ULTRAVIOLET RADIATION |
| Related Subject | AMPHIBIANS;ANIMALS;AQUATIC ORGANISMS;BACTERIA;BIOLOGICAL EFFECTS;BIOLOGICAL RADIATION EFFECTS;BIOLOGICAL RECOVERY;BIOLOGICAL REPAIR;ELECTROMAGNETIC RADIATION;GENETIC EFFECTS;GERM CELLS;MICROORGANISMS;NUCLEIC ACIDS;ORGANIC COMPOUNDS;PARASITES;RADIATION EFFECTS;RADIATIONS;RECOVERY;REPAIR;VERTEBRATES;VIRUSES |
| Description/Abstract | Repair of irradiated phage lambda DNA in E. coli has been studied by a repressed-infection system: superinfection of homoimmune lysogenic bacteria; assay for restoration of transcribility to phage-encoded lac genes; extraction of DNA and assay for infectivity in transfection of uvrB/sup -/ recA/sup -/ recB/sup -/ spheroplasts, and for removal of cyclobutane pyrimidine dimers (CBP-dimers) by UV-endonuclease treatment and alkaline sedimentation.^In uvr/sup +/ repressed infections with 254-nm irradiated phages (60 J/m/sup 2/) lac transcription was rapidly returned to undamaged levels, concomitant with restoration of infectivity and removal of CBP-dimers.^In uvrD/sup -/ cells, the frequency of phage gene inactivation corresponded to the estimated frequency of CBP-dimers per gene.^In uvrA/sup -/ bacteria, however, lac expression was only 1/10 to 1/3 of that predicted by the expected frequency of gene inactivation, as if damage elsewhere affected transcription; recovery of infectivity and removal of CBP-dimers was almost completely inhibited. lac/sup +/ and lacUV5 phages, expected to respond oppositely to changes in superhelical density, were constructed as probes for topological changes during DNA repair.^The assays for transfection infectivity and CBP-dimer-removal have been extended to studies of repair of UV-irradiated phage DNA injected into oocytes of the frog Xenopus laevis. |
| Country of Publication | United States |
| Language | English |
| Format | Pages: 1784 |
| System Entry Date | 2001 May 13 |
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Last Updated: 02/14/2009