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Poster Presentation 3-54
Allergens Produced by Drechslera (Helminthosporium) monoceras in Solid Fermentation Process Optimization and Allergen Characterization
S. D. Hasan1, W. Gambale2, R. L. Zollner3 and M. H. A. Santana1
1Biotechnological Proceses Department School of Chemical Engineering State University of Campinas DPB/FEQ/UNICAMP, CP 6066, 13083-970 Campinas-SP Brasil
2Clinical Immunology and Allergy Laboratory Medical Engineering, School of Medical Sciences State University of Campinas, Brazil
3Microbiology Department, Biomedicine Science Institute State University of São Paulo, Brazil
Telephone: 55-21-19-37883921; Fax: 55-21-19-37883918; Email: lena@feq.unicamp.br
Spores and micellium fragments of fungi are considered the most highly transmitted allergens in the air. Alternaria cladosporium, Aspergillus and Penicillium are the primary allergenic fungi genus described in literature. Previous studies using Drechslera (Helminthosporium) monoceras isolated strains from various regions of Brazil identified several glycoproteins as the main allergenic proteins from biomass cultivated in submerged fermentation. D. monoceras is a soil saprophyte and is associated with pathogenicity of plants and cereals. These characteristics indicate solid fermentation as the most suitable process for biomass cultivation. The objective of this work was to study the production of protein antigens from the biomass of D. monoceras cultivated in solid fermentation. Wheat bran was used as a substrate and the fermentation process was conducted in fixed bed columns bioreactors. The operational variables were optimized for maximum productivity by a statistical factorial design and response surface methodology. Allergen extracts were obtained by purification of crude extract through protein precipitation using ethanol and poliphenols removal by dialysis. Low and high molecular weight proteins were identified in the range of 16 to 157.3 kDa by electrophoresis. Dot blot assays were positive demonstrating the potential allergenicity of this extract.
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