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Copyright © 2005, The American Society for Cell Biology Distinct Profiles of REST Interactions with Its Target Genes at Different Stages of Neuronal Development  Marianne Bronner-Fraser, Monitoring Editor †These authors contributed equally to this work. Address correspondence to: Yuh-Man Sun (y.sun/at/bmb.leeds.ac.uk). Received July 28, 2005; Revised September 15, 2005; Accepted September 20, 2005.  This article has been corrected. See Mol Biol Cell. 2006 March; 17(3): 1494. This article has been cited by other articles in PMC. | |||||||||||||||
Abstract Differentiation of pluripotent embryonic stem (ES) cells through multipotent neural stem (NS) cells into differentiated neurons is accompanied by wholesale changes in transcriptional programs. One factor that is present at all three stages and a key to neuronal differentiation is the RE1-silencing transcription factor (REST/NRSF). Here, we have used a novel chromatin immunoprecipitation-based cloning strategy (SACHI) to identify 89 REST target genes in ES cells, embryonic hippocampal NS cells and mature hippocampus. The gene products are involved in all aspects of neuronal function, especially neuronal differentiation, axonal growth, vesicular transport and release, and ionic conductance. Most target genes are silent or expressed at low levels in ES and NS cells, but are expressed at much higher levels in hippocampus. These data indicate that the REST regulon is specific to each developmental stage and support the notion that REST plays distinct roles in regulating gene expression in pluripotent ES cells, multipotent NS cells, and mature neurons. | |||||||||||||||
INTRODUCTION Neural development proceeds via a series of commitments whereby pluripotent embryonic stem (ES) cells give rise to more restricted multipotent neural stem (NS) cells, which in turn generate differentiated neurons. This is orchestrated and accompanied by wholesale changes in transcriptional programs and patterns of gene expression. One factor that has emerged as a key player is a Krüppel-type zinc-finger transcription factor, RE1-silencing transcription factor (REST; also known as the neuron-restrictive silencing factor, NRSF). Understanding the molecular mechanism underlying the REST regulatory roles during neuronal differentiation will shed light on the process of neuronal development. The first step in decoding the REST-directed mechanism is to profile its immediate target genes, which elucidate REST's possible biological functions. REST interacts with RE1 sites and recruits corepressors including mSin3A (Huang et al., 1999 In this study, we directly investigate this hypothesis by using a novel chromatin immunoprecipitation (ChIP) cloning strategy based on selective amplification of ChIP (SACHI) to identify REST target gene interactions in ES cells, hippocampal NS cell line, MHP36 (Kershaw et al., 1994 | |||||||||||||||
MATERIALS AND METHODS Preparation of Cells, Tissues, and cDNA Embryonic stem cells, HM-1 (a gift from Dr. J. McWhir, the Roslin Institute), were cultured in Glasgow minimum essential medium (Sigma, St. Louis, MO) supplemented with 5% fetal calf serum, 5% new born calf serum, 1000 U/ml leukemia inhibitory factor (Chemicon, Temecula, CA), and the following reagents: 2 mM l-glutamine, 1% nonessential amino acids, 1 mM sodium pyruvate and 1% β-mercaptoethanol (Invitrogen, Carlsbad, CA). Neural stem cells, MHP36 (a gift from Prof. J. Price, King's College London), were plated out in 10-cm dishes precoated with 10 μl/ml fibronectin and cultured in DMEM/F12 medium in the presence of 47 μg/ml gentamicin and 200 μg/ml geneticin (Invitrogen). The medium was supplemented with 0.03% bovine serum albumin (BSA) Pathocyte (ICN, Costa Mesa, CA), 94 μg/ml human apo-transferrin, 15 μg/ml human putrescine dihydrochloride, 4.7 μg/ml bovine pancreatic insulin, 380 ng/ml l-thyroxine, 317 ng/ml tri-iodo-l-thyronine, 58 ng/ml progesterone, 1.9 mM l-glutamine, 38 ng/ml sodium selenite, 9.4 U/ml heparin sodium salt (all from Sigma), 10 ng/ml human bFGF, and 10 ng/ml murine interferon (PeproTech, Rocky Hill, NJ). Hippocampal tissues were collected by gross dissection from F1 mice (FVB/N × C57BL6) aged between 16 and 20 d. RNA was extracted using Tri reagent (Sigma) from cells or tissues. RNA was treated with DNAse (Ambion, Austin, TX) and cDNA was made from 2 μg RNA using MMLV (H-point mutant) reverse transcriptase and oligo dT and random hexamers as primers. cDNA was then purified through spin columns (Qiagen, Santa Clarita, CA) using ultra centrifugation and eluted in 50 μl of elution buffer.Neuronal Differentiation MH-1 ES cells were differentiated to NS cells and subsequently to mature neurons using monolayer culture in N2B27 medium (Ying et al., 2003  ). ES cells were plated onto 0.1% gelatin-coated cover slips in 24-well plates at a density of 1 × 104/cm2 in ES cell medium. The next day, the ES cell medium was replaced with N2B27 medium, which is a 1:1 mixture of DMEM/F12 medium supplemented with N2 and Neurobasal medium supplemented with B27 (all from Invitrogen). N2 consisted of 25 μg/ml insulin, 100 μg/ml apotransferrin, 6 ng/ml progesterone, 16 μg/ml putrescine, 30 nM sodium selenite, and 50 μg/ml BSA fraction V. Medium was changed every 2 d. Cells were processed for immunocytochemistry at day 0 (prior differentiation) and days 6, 9, and 18 after differentiation.Immunostaining Mice (C57BL6) were anesthetized with intraperitoneal Sagatal (60 mg/kg) and transcardially perfused with 4% paraformaldehyde (in 0.1 M phosphate buffer PB; pH 7.4). The brains were removed and postfixed in the same solution for 2 h at 4°C. Transverse sections (50 μm) were then cut on a vibrating microtome (Leica, Milton Keynes, United Kingdom) and collected into phosphate-buffered saline (PBS; pH 7.2). After 10-min washes three times in PBS, the sections were incubated with rabbit anti-REST primary antibody P18 (Santa Cruz Biotechnology, Santa Cruz, CA) for 12–18 h at 4°C. After 10-min washes three times in PBS, sections were incubated with a Cy3-conjugated anti-rabbit secondary antibody (1:1000 in PBS; Jackson ImmunoResearch, Luton, United Kingdom) for ~ 4 h at room temperature. These sections were then washed 10 min three times in PBS, air-dried onto glass slides, and mounted under a coverslip using VectaMount mounting medium (Vector Laboratories, Peterborough, United Kingdom). The sections were viewed under a Nikon E600 microscope (Melville, NY) equipped with epifluorescence and images obtained via an integrating analogue CCD camera (JVC KYF 55B) attached to an Acquis image capture system (Synoptics Ltd, Cambridge, United Kingdom). The fluorescent images were converted to greyscale, inverted and adjusted for brightness/contrast/intensity and color balance using CorelDraw 11 (Corel Systems, Ottawa, Ontario, Canada).ES cells, derived NS cells and neurons, and MHP36 cells were fixed in 3.5% paraformaldehyde for 20 min and stained with REST antibody (1: 300; Santa Cruz Biotechnology) alone or in combination with Sox1 (1:1000; Chemicon), Tuj1 (1:200; COVANCE, Princeton, NJ) and NeuN (1:500; Chemicon) antibodies overnight at 4°C. Chromatin Immunoprecipitation Cells (1 × 108 cells) or tissues (minced, 100 mg wet weight) were fixed for 10 min in 0.37% or 15 min in 1% formaldehyde, respectively. The fixation reaction was quenched with 0.125 M glycine. Nuclei were isolated by incubation on ice for 10 min in cell lysis buffer (10 mM Tris-HCl, pH 8.0, 10 mM NaCl, 0.2% NP40, 10 mM sodium butyrate, and protease inhibitors) and lysed in nuclei lysis buffer (50 mM Tris-HCl, pH 8.1, 10 mM EDTA, 1% SDS, 10 mM sodium butyrate, and protease inhibitors) for 10 min on ice. The lysate was then sonicated to shear the chromatin, cell debris was removed by centrifugation, and IP dilution buffer was added (20 mM Tris-HCl, pH 8.1, 150 mM NaCl, 2 mM EDTA, 1% Triton-X-100, 0.01% SDS, 10 mM sodium butyrate, and proteinase inhibitors). Chromatin was precleared for 3 h at 4°C with 200 μl of protein G-Sepharose blocked with 5% BSA. Whole cell extract was divided into aliquots: 270 μl kept as input chromatin and equal aliquots were incubated separately with anti-REST (P-18) and anti-HA (as a negative control) antibodies (Santa Cruz Biotechnology). Immune complexes were collected by rotation at 4°C for 3 h with 100 μl blocked protein G-Sepharose. Beads were washed twice with wash buffer 1 (20 mM Tris-HCl, pH 8.1, 50 mM NaCl, 2 mM EDTA, 1% Triton-X-100, and 0.1% SDS), once with wash buffer 2 (10 mM Tris-HCl, pH 8.1, 250 mM LiCl, 1 mM EDTA, 1% NP-40, 1% deoxycholic acid), and twice with TE buffer. Immune complexes were eluted with elution buffer (100 mM NaHCO3, 1% SDS) and cross-links were reversed by incubation at 65°C for 6 h in the presence of 5 μg RNase A and 0.3 M NaCl. Proteinase K, 90 μg, was added and samples were incubated overnight at 45°C. DNA was purified using phenol:chloroform extraction and ethanol precipitation. DNA from input sample was resuspended in 200 μl water, and DNA was pulled down by REST and HA antibodies in 100 μl water. Two-microliter aliquots were analyzed in real time PCR using primers designed adjacent to the RE1 regions.ChIP Cloning A cloning protocol was devised to specifically amplify RE1 containing sequences from the DNA output of anti-REST immunoprecipitated DNA. The principal of this procedure was to enrich target sequences by a nested PCR strategy using primers corresponding to the RE1 sequence and to end-ligated adaptors. The detailed procedure is summarized in Figure 1. After immunoprecipitation of fixed, sonicated chromatin but before elution of DNA from protein G-Sepharose, the sheared DNA ends were blunt-ended using mung bean nuclease, followed by adding phosphate to the 5′-ends of DNA using T4 polynucleotide kinase (BioLabs, St. Paul, MN). ChIP DNA was ligated with a specific adaptor [5′-CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGT-3′]. The RE1-containing fragments were amplified from the enriched ChIP DNA by two rounds of PCR with primer pairs. First-round PCR used an RE1 degenerate primer 5′-[TT(C)AGA(C)G(A)CCA(T)A(T)GGACAG-3′] and adaptor primer A [CTAATACGACTCACTATAGGGC-3′]. PCR cycling parameters were 95°C × 5 min; 94°C × 10 s, 52°C × 20 min, 72°C × 2 min, for 10 cycles; 94°C × 10 s, 56°C × 20 min, 72°C ×x 2 min, for 25 cycles. Second-round PCR used the RE1 degenerate primer and a nested adaptor primer B [5′-TCGAGCGGCCGCCCGGGCAGGT-3′]. Cycling conditions were 95°C × 5 min; 94°C × 10 s, 58°C × 20 min, 72°C × 2 min, for 35 cycles. Second-round PCR-products were size selected at 200–800 base pairs and cloned into the pGEM-easy vector. Seven hundred clones from each cell line were isolated and sequenced. We found that the crucial factor for designing an effective RE1 degenerate primer was to contain no degeneracy within the first six base pairs of the 3′-end.
Electrophoretic Mobility Shift Assay The ability of predicted RE1s obtained from the ChIP cloning protocol to bind REST was assayed by electrophoretic mobility shift assay (EMSA), performed as previously described (Bruce et al., 2004 ). Nuclear protein extract from U373 expressing the REST DNA-binding domain was mixed with a 32P-labeled NaV1.2 consensus RE1 probe and 100-fold molar excess unlabeled 24–28-base pair oligonucleotides, derived from cloned REST target genes, were used to compete the gel shift.Quantitative Real-time PCR Primers were designed using the primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www_slow.cgi) and “mfold” (http://www.bioinfo.rpi.edu/applications/mfold/old/dna) programs to obtain amplicons using the following parameters: Tm between 58 and 62°C, devoid of secondary structure at Tm 60°C and amplicon size of 50–150 base pairs. ChIP primers were derived from sequence within 500 base pairs of the RE1. Real-time PCR was carried out using SYBR green containing Supermix (Bio-Rad, Hercules, CA) on a Bio-Rad iCycler. All reactions were performed in duplicate. PCR cycle parameters were 95°C for 3 min, followed by 45 cycles of 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s. At the end of the program, the temperature was reduced to 50°C and then gradually increased by 1°C for 10 s up to 96°C, to produce a melt curve. Gene expression was normalized to cyclophilin using the formula 1/2ΔCt, where ΔCt = average unknown gene threshold cycle-average cyclophilin threshold cycle. The expression levels of cyclophilin in ES, MHP cells, and hippocampus showed no significant differences (Supplementary Figure 1).Data Analysis The sequences of clones obtained from ChIP cloning were blast-searched against Ensembl mouse database (http://www.ensembl.org/Multi/blastview?Species=Mus_musculus). Homologous sequences were investigated to identify the RE1 consensus: 5′-TY(TC)AGM(AC)R(AG)CCN(ACGT)NRGMCAG-3′ (Bruce et al., 2004 ). Sequences were classified according to 0, 1, 2, 3, or 4 base pairs mismatch to the consensus RE1. All fragments showing no deviation from the consensus RE1 were then annotated according to the closest genes. | |||||||||||||||
RESULTS REST Expression Profile during Neuronal Development In Vitro and In Vivo To establish the involvement of REST in neuronal development, we investigated REST expression profiles in neuronal differentiation in vitro and in vivo. The results showed that REST was expressed in both ES cells (Figure 2A) and its derived NS cells, identified by coexpression of the NS marker Sox1 (Figure 2B). Interestingly, no evident REST staining was seen in differentiated neurons either Tuj1+ or NeuN+ cells (Figure 2, C and D). REST was down-regulated in Tuj1+ cells (Figure 2C). We considered these cells as early neurons because some Tuj1+ cells were also nestin+ (unpublished data). The down-regulation of REST persisted in NeuN+ cells (Figure 2D). These observations contrast with the previous report that REST is initially down-regulated in neural stem cell, but are consistent with the observation that REST is down-regulated in early and mature neurons (Ballas et al., 2005 ). Our results suggest that, during ES cell differentiation, the expression profile of REST declines as neuronal differentiation proceeds.
However, REST mRNA and protein have been reported to be expressed in both hippocampal neurons and Tuj1+ cells derived from adult hippocampal NS cells, respectively (Kuwabara et al., 2004
ChIP Cloning ChIP has become increasingly the method of choice to identify specific targets of transcription factors. However, the relatively poor enrichment afforded by ChIP means that the vast majority of isolated DNA is not specifically bound to the transcription factor. This massive background precludes any direct cloning approach aimed at identifying novel targets. Here, we have used a selection strategy based on the use of nested PCR to specifically amplify bona fide targets. By using a degenerate RE1 sequence as one PCR primer and two nested linker primers, we were able to amplify those genomic fragments that contained RE1 consensus sequences. Next, we distinguished those sequences that represented bona fide REST targets with EMSAs using unlabeled candidate RE1s to compete the gel shift produced by incubation of the NaV1.2 consensus RE1 with U373 nuclear extract. This analysis showed that all consensus RE1s inhibited the NaV1.2 gel shift with varying affinities, whereas only 30% of those sequences containing more than 1-base pair mismatches were bound by REST (Figure 4). Accordingly, in this study, we have focused only on those REST target genes adjacent to consensus RE1s.
Identification of REST Target Genes in Embryonic Stem Cells, Neural Stem Cells, and Hippocampus Ninety-three independent clones containing consensus RE1s were isolated from ES, NS, and hippocampus using our ChIP cloning procedure. Eighty-nine of these sequences were proximal to a known or predicted transcriptional unit. Sixty RE1 sites were located within intragenic regions, of which 50% were located in the first intron. Another 25 (28%) and 7 (7.8%) RE1s were located in the 5′flanking and 3′flanking regions, respectively (Tables 1 and 2). Four genes comprising L1cam, Syt2, GEF1, and ENSMUSG00000041339 were adjacent to, or contained, two RE1 sites. Thirty-eight genes (42%) were annotated in the Ensembl database (Supplementary Table 1) and were classified into six groups according to their biological functions (Table 1), whereas the remaining 51 genes (58%) corresponded to novel, predicted transcriptional units (Table 2). Of the annotated genes, 24 (65%) are expressed specifically or selectively in the nervous system (Table 1), including those concerned with neurite outgrowth, axonal guidance, vesicle trafficking, signaling, and transcription, consonant with the role of REST in regulating neuronal gene expression. The fact that more than 85% of these genes have not previously been shown to be direct targets of REST is testament to the power of this ChIP cloning protocol to reveal novel target genes.
Validation of Bona Fide REST Target Genes Validation of REST target genes isolated from ChIP cloning was carried out using conventional ChIP analysis. Thirty-three genes were randomly selected for testing from the isolated 89 genes and included 23 annotated genes and 10 predicted genes. Thirty-two genes (97%) were occupied by REST and showed greater than 1.5-fold enrichments in ChIP assays, in at least one of the three cell types (Figure 5). Four genes comprising Resp18 and ENSMUSG00000038457 in NS cells, Zfm1 in ES cells, and NM_173446 in NS cells and hippocampus exhibited markedly high enrichments of 18-, 20-, 46-, 36-, and 12.6-fold, respectively.
The results showed that the equivalent RE1 sites were differentially bound by REST in ES, NS cells, and hippocampus. RE1 sites associated with Ntrk3, ENMUSG000000 41339/17k, Olfr1336, ENSMUSESTG00000031508, CREBBP/EP300 inhibitory protein 2, Accn1, Arc, Atp2b2, and Zfm1 genes were bound by REST in ES and NS cells, but not in hippocampus, whereas the RE1 sites associated with Drd2, ENSMUSG00000028804, Syt2, ENSMUSG00000015719, and Kirrel3 genes were bound by REST in ES and hippocampus, but not in NS cells. Conversely, RE1 sites associated with NM_175461, Apba2, and NM_173446 genes were bound by REST in NS cells and hippocampus, but not in ES cells. Furthermore, some RE1 sites were bound by REST in only one cell type including Pkd1l3 and Mbp genes in ES cells, the Cacna2d3 gene in NS cells, and Kcnh1 and Cspg3 genes in hippocampus. Only one RE1 site, which was located 30 kb upstream from the coding region of Cspg4 gene, failed to show any occupancy by REST in any cell type. Collectively, these data show that each cell type bears a unique REST occupancy signature. Gene Expression Profile of the REST Differentially Targeted Genes To examine the relationship between REST occupancy and expression of the corresponding gene, we carried out real-time quantitative PCR to determine the expression profile of REST target genes in each cell type. Of the 33 verified REST target genes, 20 were randomly selected to determine their expression pattern. One striking difference was evident in the contrast of the expression profile seen in ES and NS cells compared with that seen in the hippocampus. Although most genes were either silent or expressed at relatively low level in both ES and NS cells, with one exception, all genes were expressed at relatively high levels in hippocampus (Table 3). In ES cells, only the Olfr1336 was silent. In NS cells, 10 of the 20 genes were silent, whereas CREBBP/EP300 inhibitory protein 2, Kirrel3, and ENSMUSG00000051719 genes were expressed at relatively high levels. In hippocampus, only the olfactory receptor gene, Olfr1336, was silent. All other genes, including the five unannotated predicted genes were expressed at high levels relative to ES and NS cells.
Most of these target genes are reported to be specifically or selectively expressed in neurons. Exceptions include CREBBP/EP300 inhibitory protein 2 and Zfm1, which are widely expressed and Kirrel3, which is expressed in both neural and stromal cells. The fact that REST and so many target genes are coexpressed in the hippocampus, further underscores the principle role of REST in the adult CNS as a regulator, but not a silencer of neuronal gene expression. | |||||||||||||||
DISCUSSION Transcription factors play crucial roles in all aspects of cellular function including cell proliferation, differentiation, and survival. Yet, a key issue remains identification of their target genes. It is anticipated that the repertoire of target genes operated on by any given transcription factor will vary according to cell type and developmental stage. Despite this assumption, we have very little knowledge of the cell-specific regulon for any transcription factor in the genome of higher eukaryotes. Several strategies have evolved to identify transcription factor targets including ChIP-chip (Lee et al., 2002 REST Target Genes and Neuronal Function Many REST target genes involved in regulating core aspects of neuronal phenotype such as vesicular transport and release, signaling, and neurite outgrowth. Several known REST target genes are involved in neurite outgrowth and/or axonal guidance through regulation of intercellular and cell matrix interactions and of the intracellular cytoskeleton and include L1cam, Cdh4, Adam23, Catnd2, Ppp2r2c, and Unc5d (Gerhardt et al., 2000 ; Kim et al., 2002 , Strack, 2002 ; Nishimura et al., 2003 ; Frappé et al., 2004 ; Goldsmith et al., 2004 ; Liu et al., 2004 ). This list can now be expanded by the addition of a further four REST target genes, Cspg3, Shank2, Extl3, and Arc, all of which are involved in regulation of cell adhesion or modulation of the cytoskeleton (Fujimoto et al., 2004 ; Osman et al., 2004 ; Qualmann et al., 2004 ). This cohort of target genes lends mechanistic insight into the axon pathfinding errors resulting from constitutive expression of REST (Paquette et al., 2000 ). Two new targets regulating vesicular trafficking and release include Rab4a and Apba2. These add to the known REST targets, Snap25, Syt2, Syt7, synapsin 1, and synaptophysin and illustrate the ability of REST to regulate every step of neurosecretion from trafficking, through docking to fusion and release.REST Interacts with the Chromatin of Transcriptionally Active Genes in Differentiated Neurons Our results show that REST occupies distinct subsets of target genes in ES cells, NS cells and hippocampus. A total of 89 genes were identified in this study, 38 of which are annotated, whereas 51 have no annotation and represent hypothetical genes. Of the 38 annotated REST target genes 24 (65%) encode gene products that are specifically or selectively expressed in neural tissue. Most target genes are transcribed at high levels in the hippocampus compared with ES and NS cells.All target genes selected for expression analysis were transcribed in hippocampus, with the exception of Olfr1336. Most of the RE1 sites of these genes are occupied by REST. How does REST act principally as a repressor and become an activator in hippocampus? A previous study showed that NRSE dsRNA (RE1 dsRNA) appeared in the early stage of neurogenesis and in hippocampus. During the differentiation of NS cell to neurons, NRSE dsRNA triggered gene expression of neuron-specific genes through interaction with REST transcriptional machinery, by which REST was converted from a repressor to an activator (Kuwabara et al., 2004 REST Interaction with Target Genes in Stem Cells In contrast to the hippocampus, the majority of target genes are silent in MHP36 neural stem cells and most are occupied by REST, irrespective of their transcriptional status. The only unoccupied sites lie within the Cspg3, Kcnh1, Drd2, and Kirrel 3 genes. The profile of REST occupancy in ES cells is very similar to that of MHP36 cells but unlike MHP36 cells, most REST target genes are transcribed in ES cells, albeit at low levels compared with the hippocampus. As with MHP36 cells, Cspg3, and Kcnh1 are both silent and occupied by REST. This low level of transcription likely reflects a transcriptionally poised state whereby the chromatin is an open configuration before full transcriptional activation upon differentiation (Schubeler et al., 2000 ; Ragoczy et al., 2003 ). In general, this study shows that many REST target genes are either silent or weakly expressed in ES and MHP36 cells but are strongly expressed in hippocampus. This may imply that REST is acting to silence or strongly repress these genes in both multipotent and pluripotent cells and then switches to become a regulator in postmitotic differentiated neurons.A recent study reported that REST was present in ES cells but upon retinoic acid differentiation into neurons, REST was rapidly down-regulated in NS cells and was absent from both cultured cortical progenitors and cortical neurons (Ballas et al., 2005 In summary, we have used a novel ChIP cloning protocol (SACHI) to identify 89 REST target genes. We show that the REST occupies a distinct set of target genes in ES cells, NS cells and hippocampal neurons and that occupancy is not simply related to expression levels. The results suggest that the REST-directed mechanism in the gene expression of its target genes extends beyond the simple “on/off” model and the mechanism may be compounded by combinatorial regulation from other transcription factors or corepressors. | |||||||||||||||
[Supplemental Material]
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Acknowledgments This work was funded by the Wellcome Trust. | |||||||||||||||
Notes This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E05–07–0687) on September 29, 2005. Abbreviations used: RE1, repressor element 1; NRSE, neuron-restrictive silencer element; REST, repressor element 1 silencing transcription factor; NRSF, neuron-restrictive silencing factor; ES, embryonic stem cell; NS, neural stem cell; EMSA, electrophoretic mobility shift assay. The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org). | |||||||||||||||
References
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