Baylor University, Waco, TX, B.S.,1968, Chemistry
University of Houston, Houston, TX, Ph.D., 1975, Analytical Chemistry

Experience:

• 1979-Present, Mass Spectrometrist/Analytical Chemist, U.S. Govt.; FDA.
• 1977-1979, Mass Spectrometrist/Analytical Chemist, U.S. Govt.; Dept. Defense
• 1976-1977, Mass Spectrometrist; Support Staff, University of Hawaii, Honolulu, HI
• 1975-1976, Postdoctoral Welch Fellow; Marine Biomedical Institute, Galveston, TX
• 1969-1971, Military Service; U.S. Army, Signal Corps
• 1968-1969, Chemist, Philips Petroleum, Sweeney, TX
• 1966-1968, Undergraduate Research Assistant; Baylor University, Waco, TX
• 1962-1964, Science Assistant; Riesel High School, Riesel, TX

Honors:

• NCTR Employee of the Month for June 2000
• NCTR Outstanding Government Employee Award (May 19, 2000) "for exemplary performance and commitment to providing invaluable mass spectrometry analytical service support for NCTR investigators and visiting scientists by sharing his excellent knowledge, skills and expertise."
• NCTR Cash Award (August 16, 1999) "for special accomplishments in providing a considerable cost savings to the NCTR by repairing a critical mass spectrometer."
• NCTR Cash Award (January 26, 1998) "for the careful evaluation of the proposals for the purchase of a liquid chromatography/mass spectrometer which provided the information needed to make an effective decision on the purchase."
• NCTR Cash Award (May 29, 1997) "for creating a database for the Division of Facilities Engineering and Maintenance which will track expenditures...."
• NCTR On-the-Spot Cash Award (January 10, 1997)
• Sigma Xi Certificate of Recognition (FDA Chapter; April 1991) "for an outstanding poster presentation at the Third Annual FDA Science Poster Expo."
• NCTR Cash Award (1991) "for outstanding service on the 1990 Board of Survey Team."
• NCTR Cash Award (1991) "for obtaining and transporting from various agencies more than $100,000 worth of surplus equipment and supplies for distribution center-wide at the NCTR."
• Friend of the Department of Chemistry, University of Arkansas at Little Rock, 1988.
• FDA Commendable Service Award (1987) "for continued excellence in maintaining NCTR's mass spectrometry facility and for providing starte-of-the-art mass spectral service."

• For the past 35 years, I have been involved in the physical sciences and scientific laboratories, from the high school level to national research. For the last 25+ years, I have maintained and operated mass spectrometers, but I have also been involved in the setting up of more conventional chemical laboratories. I also have considerable experience in the fields of laboratory computers, based on university courses and actual use of computers for the control/acquisition/data processing stages of mass spectrometry. This computer experience encompasses the scope from mainframe to personal computers.
• In my present employment (23 yrs), I function as a service mass spectrometrist in a multi-instrument laboratory consisting of seven mass spectrometers for LCMS, GCMS, MS/MS, pyrolysis MS and LD/TOF MS. I have responsibility for the acquisition of new instruments, operation, repair, and modification of present equipment; and the training of new employees, as well as the analysis of samples submitted for identification and/or purity estimates. My achievements have earned me promotions to my present grade of GS-14 (1992) and awards such as the FDA Commendable Service Award (1987) and an NCTR Outstanding Government Employee Award (2000).
• In conjunction with researchers, both at work and from nearby universities, I have performed mass spectral analyses that resulted in numerous publications. I have also presented original research at the annual American Society for Mass Spectrometry (ASMS) meetings. My broad background and technical experience have allowed me to function as a technical consultant not only at the work site, but also for Federal, State and Academic laboratories in this region.

Most Recent Publications (1995+):

• Wilkes, J.G., Freeman, J.P., Heinze, T.M., Lay, Jr., J.O., and Vestel, M.L., "AC-Corona-discharge Aerosol-neutralization Device Adapted to Liquid Chromatography/Particle Beam/Mass Spectrometry", Rapid Comm. Mass Spectrom., 9, 138-42,1995.

• Pothuluri, J.V., Selby, A.L., Evans, F.E., Freeman, J.P. and Cerniglia, C.E., "Transformation of chrysene and other polycyclic aromatic hydrocarbon mixtures by the fungus Cunninghamella elegans", Canadian J. Botany, 73(1), S1025-S1033,1995.
  
• Zhang, D., Evans, F.E., Freeman, J.P., Duhart, Jr, B., and Cerniglia, C.E., "Biotransformation of Amitriptylene by Cunninghamella elegans", Drug Metabolism & Disposition, 23, 1417-1425, 1995.

• Sutherland, J.B., Tanner, L.A., Moore, J.D., Freeman, J.P., Deck, J., and Williams, A.J., "Conversion of Ferulic Acid to 4-Vinylguaiacol by Yeasts Isolated from Frozen Concentrated Orange Juice", J. Food Protection, 58, 1260-1262, 1995.

• Samokyszyn, V.M., Freeman, J.P., Maddipati, K.R., Lloyd, R.V., "Peroxidase-catalized oxidation of pentachlorophenol", Chemical Research in Toxicology, 8, 349-355, 1995.

• Zhang, D, Freeman, JP, Sutherland, J.B,, Walker, AE, Yang, Y and Cerniglia, CE, "Biotransformation of Chlorpromazine and Methdilazine by Cunninghamella elegans", Appl. Environ. Microbiol., 62, 798-803, 1996.

• Sutherland, JB, Evans, FE, Freeman, JP and Williams, AJ, "Biotransformation of quinoxaline by Streptomyces badius", Letters in Applied Microbiology, 22, 199-201, 1996.

• Ang, C.Y.W., Luo, W., Hansen, Jr., E.B., Freeman, J.P., and Thompson, Jr., H.C., "Determination of Amoxicillin in Catfish and Salmon Tissues Using HPLC with Pre-column Formaldehyde Derivatization", J. AOAC Int., 79, 389-396, 1996.

• Chang, H.C., Compadre, R.L., Lloyd, R.V., Freeman, J.P. and Samokyszyn, V.M., "Nonenzymatic Reduction of Tetrachloro-1,4-Benzoquinone by Reduced Nicotinamide Adenine Dinucleotide Phosphate in an Aqueous System", Biochem & Biophys. Res. Comm., 220, 1043-1048, 1996.

• Bezalel, L., Hadar, Y., Fu,P.P., Freeman, J.P., and Cerniglia, C.E., "Metabolism of Phenanthrene by the White Rot Fungus Pleurotus ostreatus", Appl. Environ. Microbiol., 62, 2547-2553, 1996.

• Bezalel, L., Hadar, Y., Fu,P.P., Freeman, J.P., and Cerniglia, C.E., "Initial Oxidation Products in the Metabolism of Pyrene, Anthracene, Fluorene, and Dibenzothiophene by the White Rot Fungus Pleurotus ostreatus", Appl. Environ. Microbiol., 62, 2554-2559, 1996.

• McClure, GYH, Freeman, JP, Lay, JO, Hinson, JA, "Haemoglobin Adducts Biomarkers of Exposure to the Herbicides Propanil and Fluometron", Biomarkers, 1, 136-140, 1996.

• Raza, H, Squires, RB, Guengerich, FP, Miller, DW, Freeman, JP and Kadlubar, FF, "Metabolism of 2-amino-a-carboline, a Food-borne Heterocyclic Amine Mutagen and Carcinogen, by Human and Rodent Liver Microsomes and by Human Cytochrome P4501A2", Drug Metabolism, 24, 395-400, 1996.

• Zhang, Z, Evans, FE, Freeman, JP, Yang, Y, Deck, J and Cerniglia, CE, "Formation of Mammalian Metabolites of Cyclobenzaprine by the Fungus, Cunninghamella elegans", Chem-Biol Interactions, 102, 79-92, 1966.

• Luo, W., Hansen, E.B., Ang, C.Y.W., Deck, J., Freeman, J.P., Thompson, H.C., "Simultaneous Determination of Amoxicillin and Ampicillin in Bovine Milk by HPLC with Fluorescence Detection", J. Agric. Food Chem., 45, 1264-1268, 1997.

• Samokyszyn, V.M., Freyaldenhoven, M.A., Chang, H.C., Freeman, J.P., Compadre, R.L., "Regiospecifity of Peroxyl Radical Addition to (E)-Retinoic Acid", Chem. Res. in Toxicology, 10, 795-801, 1997.

• Kim, E. Zylstra, GJ, Freeman, JP, Heinze, TM, Deck, J, Cerniglia, CE, "Evidence for the Role of 2-hydroxychromene-2-carboxylate Isomerase in the Degradation of Anthrancene by Sphingomonas yanoikuyae B1", FEMS Microbiology Letters, 153, 479-484, 1997.

• Moran, JH, Weise, R, Schnellmann, RG, Freeman, JP, Grant, DF, " Cytotoxicity of Linoleic Acid Diols to Renal Proximal Tubular Cells", Toxicol. Appl. Pharm., 146, 53-59, 1997.

• Wiese, FW, Chang, HC, Lloyd, RV, Freeman, JP, Samokyszyn, VM, "Peroxidase-Catalyzed Oxidation of 2,4,6-Trichlorophenol", Arch. Environ. Contam. Toxicol., 34, 217-222, 1998.

• Sutherland, JB, Freeman, JP, Williams, AJ, "Biotransformation of Isoquinoline, Phenanthridine, Phthalazine, Quinazoline, and Quinoxaline by Streptomyces viridosporus", App. Microbiol. Biotechnol., 49, 445-449, 1998.

• Pothuluri, JV, Sutherland. JB, Freeman, JP, Cerniglia, CE, "Fungal Biotransformation of 6-Nitrochrysene", Appl. Environ. Microbiol., 64, 3106-3109, 1998.

• Sutherland, JB, Freeman, JP, Williams, AJ, Deck, J, "Metabolism of Cinnoline to N-oxidation Products by Cunninghamella elegans and Aspergillus niger", J. Industrial Microbiol. Biotechnol., 21, 225-227, 1998.

• Sutherland, JB, Freeman, JP, Williams, AJ, Deck, J, "Biotransformation of Phthalazine by Fusarium monofiliforme and Cunninghamella elegans", Mycologia, 91 (1), 114-116, 1999.

• Pothuluri, JV, Freeman, JP, Fu, PP, Cerniglia, CE, "Biotransformation of 1-Nitrobenzo[e]pyrene by the Fungus Cunninghamella elegans", J. Industrial Microbiol. Biotechnol., 22, 52-57, 1999.

• Duhart, BT, Zhang, D, Deck, J, Freeman, JP, Cerniglia, CE, "Biotransformation of Protriptyline by Filamentous Fungi and Yeasts", Xenobiotica, 29, 733-746, 1999.

• Parshikov, IA, Freeman, JP, Lay, JO, Beger, RD, Williams, AJ, Sutherland, JB, "Regioselective Transformation of Ciprofloxacin to N-Acetylciprofloxacin by the Fungus Murcor ramannianus", FEMS Microbiology Letters, 177, 131-135, 1999.

• Moody, JD, Freeman, JP, Cerniglia, CE, "Biotransformation of Doxepin by Cunninghamella elegans", Drug Metabolism and Disposition, 27, 1157-1164, 1999.

• Parshikov, IA, Freeman, JP, Williams, AJ, Deck, J, Sutherland, JB, "Biotransformation of N-Acetylphenothiazine by Fungi", Appl. Environ. Microbiol. 52, 553-557, 1999.

• Parshikov, IA, Freeman, JP, Lay, JO, Beger, RD, Williams, AJ, Sutherland, JB, "Microbiological Transformation of Enrofloxacin by the Fungus Murcor ramannianus", Applied & Environ. Microbiol., 66, 2664-2667, 2000.

Proceedings List (1995 +):

• Wilkes, JG, Voorhees, KJ, Freeman, JP, Volmer, DA, Lay, Jr., JO, "Two Vintage Quadrupole Mass Spectrometers for Coordinated Pyrolysis-MS", Proceedings of the 43rd. ASMS Conference on Mass Spectrometry & Allied Topics, 43, 1995.

• Wilkes, JG, Voorhees, K, Rafii, F, Sutherland, JB, Lay, JO, and Freeman,JP, "Factors which affect the Reproducibility of Pyrolysis Mass Spectra for Microbiological Samples", Proceedings of the 44th. ASMS Conference on Mass Spectrometry & Allied Topics, 44, 589, 1996.

• Luo, W., Hansen, E.B., Ang, C.Y.W., Deck, J., Freeman, J.P., Thompson, H.C., "Simultaneous Determination of Amoxicillin and Ampicillin in Bovine Milk by HPLC with Fluorescence Detection", Proceedings Amer. Chem. Soc. , 1996.

• Wilkes, JG, Heinze, TM, Freeman, JP, Rafii, F, Cyr, WH, Voorhees, KJ, "Use of Probe Sample Introduction with EI or MAB Ionization for Rapid Bacterial Chemotaxonomy", Proceedings of the 45th. ASMS Conference on Mass Spectrometry & Allied Topics, 45, 1997.

• Wilkes, JG, Freeman, JP, Colby, S, Akbar, N, Lay, JO, Abramson, FP. "Fundamentals in the Development of an LC/CRIMS System", Proceedings of the 45th. ASMS Conference on Mass Spectrometry & Allied Topics, 45, 1997.

• Wilkes, JG, Colby, S, Abramson, FP., Freeman, JP, "Developing a Commercial HPLC/CRIMS System", Proceedings of the 46th. ASMS Conference on Mass Spectrometry & Allied Topics, 46, 1998.

• Billedeau, SM, Holland, RD, Heinze, TM, Freeman, JP, Cooper, WM, Kuperman, A, Lay, JO, " Analysis of Tobacco Specific N-Nitrosamines (TSNAs), NNN and NNK, in Tobacco by HPLC/Particle Beam/TEA, HPLC/ESI/MS and GC/EI/MS", Proceedings of the 48th. ASMS Conference on Mass Spectrometry & Allied Topics, 48, 2000.

Meeting Abstracts (1995 +)

• Fifer, EK, Robertson, KS, Freeman, JP, Shaikh, AU, "Ortho-substituted Nitrophenylfurans: Chemistry and Mutagenicities", Amer. Chem. Soc. April 1995.

• Zhang, D, Freeman, JP, Cerniglia, CE, "Oxidative Metabolism of Methdilazine by Cunninghamella elegans", Amer. Soc. Microbiology, May 1995.

• Sutherland, JB, Freeman, JP, Evans, FE, Williams, AJ, "Biotransformation of Quinoxaline by Strepomyces badius", Amer. Soc. Microbiology, May 1995.

• Sutherland, JB, Tanner, LA, Moore, JD, Freeman, JP, Deck, J, Williams, AJ, "Conversion of Ferulic Acid to 4-vinylguaiacol by Yeasts Isolated from Frozen Orange Juice", Mycol. Soc. of America & Amer. Instit. of Biolog. Sciences, August 1995.

• Lou, W, Ang, YWC, Hansen, EB, Freeman, JP, Thompson, HC, "Determination of Amoxicillin in Catfish and Salmon Tissues Using HPLC with Precolumn Formaldehyde Derivatization", AOAC, 1995.

• Sutherland, J.B., Freeman, J.P., Williams, A.J., "Oxidation of the Heterocyclic Rings of Azaarenes by Streptomyces viridosporus", Amer. Soc. Microbiology, May 1996.

• Sutherland, J.B., Freeman, J.P., Williams, A.J., Heinze, TM, "Oxidation of Phenothiazine by the Fungus Cunninghamella elegans", Amer. Soc. Microbiology, May 1997.

• Sutherland, J.B., Freeman, J.P., Williams, A.J., Heinze, TM, Zhang, D, "Microbial Model for Phenothiazine Metabolism Using Cunninghamella elegans", FDA Science Forum, 1997.

• Wilkes, JG, Freeman, JP, Madabushi, J, Hutchinson, MW, Stearns, SD, Colby, SM, "A Novel HPLC Detection System", PittCon 1998.

• Sutherland, JB, Freeman, JP, Williams, AJ, Deck, J, "Oxidation of Cinnoline to Two Isomeric N-Oxides by the Fungi Cunninghamella elegans and Aspergillus niger", Amer. Soc. Microbiology, May 1998.

• Sutherland, JB, Freeman, JP, Williams, "Biotransformation of Phthalazine by Fusarium moniliforme and Cunninghamella elegans" Mycological Soc. of America, June, 1998.

• Sheets, JL, Whitfield, S, Shaikh, Au, Freeman, JP, Fifer, EK, "4-Nitrobiphenyls: Synthesis and Mutagenicities", Ark. Acad. Science, April, 1998.

• Parshikov, IA, Freeman, JP, Williams, AJ, Moody, JD, Sutherland, JB, "Biotransformation of N-Acetylphenothiazine by Fungi", Amer. Soc. Microbiology, May 1999.

• Sutherland, JB, Freeman, JP, Williams, AJ, Moody, JD, "Oxidation of Phenoxazine by the Fungus Cunninghamella elegans", Amer. Soc. Microbiology, May 1999.

• Pothuluri, JV, Harris, B, Freeman, JP, Heinze, TM, Beger, RD, Cerniglia, CE, "Fungal Biotransformation of the Fungicide, Vinclozolin", Amer. Soc. Microbiology, May 2000.

• Hur, H-G, Lay, JO, Beger, RD, Freeman, JP, Rafii, F, "Isolation of Intestinal Anaerobic Bacteria Capable of Metabolizing the Neutral Isoflavonoid, Daidzin", Amer. Soc. Microbiology, May 2000.

• Parshikov,IA, Freeman, JP, Lay, JO, Williams, AJ, Moody, JD, Sutherland, JB, "Formation of Unusual Conjugates from Ciprofloxacin and Norfloxacin by the Fungus Trichoderma viride", Amer. Soc. Microbiology, May 2000.

• Parshikov,IA, Lay, JO, Freeman, JP, Beger, RD, Williams, AJ, Sutherland, JB, "Microbiological Transformation of Enrofloxacin by the Fungus Mucor ramannianus", Amer. Soc. Microbiology, May 2000.

• Parshikov,IA, Freeman, JP, Lay, JO, Beger, RD, Williams, AJ, Sutherland, JB, "Metabolism of the Veterinary Fluoroquinolone Sarafloxacin by Mucor ramannianus", Amer. Soc. Microbiology, May 2000.