PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,
caution:Cys-250 is present instead of the conserved Lys which is expected to be an active site residue. Lys-233 appears to fulfill the required catalytic function.,
cofactor:Magnesium.,
enzyme regulation:By hypertonicity. Activation requires autophosphorylation of Ser-382. Phosphorylation of Ser-378 also promotes increased activity.,
function:Controls sodium and chloride ion transport by inhibiting the activity of WNK4, potentially by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization.,
similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.,
similarity:Contains 1 protein kinase domain.,
subcellular location:Cytoplasm.,
subunit:Interacts with SYT2.,
tissue specificity:Widely expressed in both adult and embryonic tissue, with highest levels observed in the testis and lower levels in heart, lung, kidney, placenta, brain and skeletal muscle. Two isoforms are expressed in heart, a single shorter isoform in the kidney. Locates to the distal convoluted tubule, the medullary collecting duct and the cortical collecting duct of the kidney.,
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