Cell_Type | MW_unreduced | MW_reduced | Comment |
---|---|---|---|
Erythrocytes | 95-110kDa | 95-110kDa | Smear due to heterogeneous glycosylation |
Enzyme | Comment |
---|---|
p72syk | Tyrosine kinase phosphorylation of band 3 {Harrison_ML/Low_PS.1994.JBC} |
casein kinase I | Threonine phosphorylation of band 3 {Wang_CC/Low_PS.1997.BLOOD} ** |
Molecule | Comment |
---|---|
glycophorin A | Band 3 interacts with glycophorin A, and this interaction facilitates the movement of band 3 to the cell surface. The interaction also results in the formation of the Wrb blood group antigen {Groves_JD/Tanner_MJ.1992.JBC} ; {Bruce_LJ/Tanner_MJ.1995.BLOOD} |
ankyrin | The tetrameric form of band 3 attaches to the red cell cytoskeleton via ankyrin {Willardson_BM/Low_PS.1989.JBC} |
protein 4.1 | Protein 4.1 modulates the band 3-ankyrin interaction {An_XL/Mohandas_N.1996.JBC} |
protein 4.2 | Protein 4.2 strengthens the band 3-cytoskeleton link {Rybicki_AC/Cobb_CE.1996.BLOOD} |
carbonic anhydrase II | Carbonic anhydrase II binds to the carboxyl terminus of band 3 {Vince_JW/Reithmeier_RA.1998.JBC} |
hemoglobin | Hemoglobin interacts with the cytoplasmic domain of band 3 {Walder_JA/Arnone_A.1984.JBC} |
glycolytic enzymes | The activity of glycolytic enzymes is regulated by binding to band 3 {Low_PS/Harrison_ML.1993.JBC} |
Species_or_Strain | Supplier | Comment |
---|---|---|
Band 3 (-/-) | Not commercially available | {Peters_LL/Lux_SE.1996.CELL} |
mAb | Supplier | Comment |
---|---|---|
Anti-Band 3, Human | Sigma-Aldrich ( B9277 ) | The antibody recognizes an epitope located in the cytoplasmic N-terminus of the band 3 molecule. Clone no. BIII-136. Indirect immunofluorescence using methanol-fixed human erythrocytes. Immunoprecipitation using human erythrocytes. 1:5,000 by Immunoblotting (colorimetric) using human erythrocytes {Czerwinski_M/Lisowska_E.1988.EJB} |
Anti-Human Red Cell Wrb Antigen | Sigma-Aldrich ( R4011 ) | The antibody binds to Wrb, a composite blood group antigen resulting from the association between glycophorin A and band 3 {Telen_MJ/Haynes_BF.1987.VS} . The antibody, clone no. E6 (also cited as clone no. 7H12), binds to 9% of bone marrow nucleated cells in smears and tissue preparations using immunofluorescent microscopy or flow cytometry. 1:10 by Agglutination assay using human erythrocytes. Flow cytometry using bone marrow nucleated cells. Indirect immunofluorescence using bone marrow nucleated cells |
Molecule | Supplier | Comment |
---|---|---|
Stilbene disulphonates | Sigma-Aldrich | Stilbene disulphonates bind to extracellular lysine residues {Okubo_K/Jennings_ML.1994.JBC} |
5-maleimido-eosin | Fluka subsidary of Sigma-Aldrich | Eosin maleimide binds to lysine 430 {Liu_SQ/Knauf_PA.1993.AJP} |
pyridoxal phosphate | Sigma-Aldrich | Pyridoxal phosphate binds to extracellular lysine residues {Kawano_Y/Hamasaki_N.1988.JBC} |
2,3-bisphosphoglycerate | Sigma-Aldrich | 2,3-bisphosphoglycerate binds band 3 and inhibits association with protein 4.1 and ankyrin {Moriyama_R/Low_PS.1993.JBC} ** |
calcium | Sigma-Aldrich | Calcium binds and inhibits anion exchange** |