De Lucca AJ, Jacks TJ, Bland JM, Grimm C, Cleveland TE, Walsh TJ; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1996 Sep 15-18; 133 (abstract no. F192).
Southern Regional Research Center, New Orleans, LA.
In vitro antifungal properties of Cecropin B (CB) and dermaseptin (DERM), potent antibacterial peptides from the giant silk moth and frog skin, respectively, were explored. Nongerminated and germinated (8 hr) conidia from Aspergillus flavus (AF), A. fumigatus (AFUM), A. niger (AN), Fusarium moniliforme (FM) and F. oxysporum (FO) were incubated (30 min, 30 degrees Celsius) separately with the peptides. Aliquots were inoculated onto FDA plates, incubated (30 degrees Celsius, 24 hrs) and colonies enumerated. Neither peptide reduced conidial viabilities of nongerminated Aspergillus sp. CB produced LD(50) values for germinated AF, AFUM and AN conidia of 3.0, 0.5 and 2.0 micromolar, respectively, while DERM gave LD(50) values of 4.0, 0.05 and 2.0 micromolar, respectively. CB gave LD(50) values of 0.2 micromolar for nongerminated FM and FO conidia, while DERM only slightly reduced viabilities of both Fusarium species. LD(50) levels for CB were 0.2 and 0.1 micromolar, respectively, for germinated FM and FO conidia. DERM was less effective, giving LD(50) values for germinated FM and FO conidia of 0.3 and 0.8 micromolar, respectively. Physico-chemical studies indicated CB, but not DERM, bound to ergosterol, a constituent of fungal cell walls. Neither peptide complexed with chitin or beta-1,3-glucan. Results show that antifungal properties of these peptides were genus and species dependent.
Publication Types:
Keywords:
- Amphibian Proteins
- Antifungal Agents
- Antimicrobial Cationic Peptides
- Aspergillus
- Ergosterol
- Fungi
- Fusarium
- Glucans
- In Vitro
- Insect Proteins
- Miconazole
- Niger
- Peptides
- Spores, Fungal
- cecropin B protein, Insecta
- dermaseptin
Other ID:
UI: 102234885
From Meeting Abstracts