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Document Number | 213 |
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Primary Title | Enzymology Research |
Author Name | Himmel, M. E.;Grohmann, K. |
Author Affiliation | Solar Energy Research Inst., Golden, CO |
Published Date | 11/01/1988 |
Detailed Publish Date | Nov 1988 |
Title Source | FY 1987 Biochemical Conversion/Alcohol Fuels Program. Annual Report |
Page Range | B.165 |
Page Count | 1 |
Document Type | REPORT CHAPTER |
Publisher Name | Solar Energy Research Inst., Golden, CO |
Abstract | Enzymology research at SERI in FY 1986-87 has focused on the problem of enhancing the effective lifetime of the key fungal cellulase enzymes under process conditions, since the cost of producing these enzymes has been identified as a sensitive issue in the overall bioconversion economics. The effective utilization of cellulase hydrolysis potential in these process schemes requires access to enzymes of high stability and a scheme for recovery of this activity before disposal of spent feedstock. The issue of adsorptive loss of cellulase activities was studied in FY 1987 and is reported here. Also reported in this document are studies designed to quantitatively describe the instability of the native fungal beta-glucosidase and to then investigate chemical crosslinking technology as a toll for achieving greater stability. In the process of performing this work a new affinity chromatography method was developed and is also described herein. Progress was also made in FY 1987 on the characterization of the highly thermostable cellulase enzymes secreted by the new thermophile Acidothermus cellulolyticus and these studies are reported as well. |
Report Number | SERI/SP-231-3245 |
Copyright Status | N - Not copyrighted, |
Document Source | NTIS, Order No. DE89000831 |
Document Owner | B |