Methods in Enzymology

Table of ContentsMethods in Enzymology

Contributors to Volume 225 xi

Preface xvii

Volumes in Series xix

Hints on Using the Guide to Techniques in Mouse Developentxxxiii

    Section I. General Methodology

  1. Recordkeeping and Database Analysis of Breeding Colonies
    Lee M. Silver 3
  2. Anethesia and Perioperative Care
    P.A. Flecknell 16

    3. Section II. Germ Cells and Embryos

  3. Culture and Manipulation of Primordial Germ Cells
    Julie E. Cooke, Isabelle Godin, Charles French-Constant, Janet Heasman, and Christopher C Wylie 37
  4. Insolation and Culture of Primordial Germ Cells
    Mia Buehr and Anne McLaren 58
  5. Isolation and Culture of Oocytes
    John J. Eppig and Evelyn E. Telfer 77
  6. Purification, Culture, and Fractionation of Spermatogenic Cells
    Anthony R. Bellvé 84
  7. Recovery, Capacitation, Acrosome Reaction, and Fractionation of Sperm
    Anthony R. Bellvé, Wenxin Zheng, and Yordanka S. Martinova 113
  8. Strategies and Methods for Evaluating Acrosome Reaction
    Richard A. Cardullo and Harvey M. Florman 136
  9. Culture of preimplantation Embryos
    Joel A. Lawitts and John D. Biggers 153
  10. Isolation and Culture of Whole Postimplantation Embryos and Germ Layer Derivatives
    Karin Sturm and Patrick P.L. Tam 164
  11. Histogenetic Potency of Embryonic Tissues in Ectopic Sites
    Patrick P.L. Tam 190

    12. Section III. Fertilzation

  12. Micromanipulation of Gametes and Embryos
    Jon W. Gordon 207
  13. In Vitro Capacitation and Fertilization
    Lynn R. Fraser 239
  14. In Vitro Fertilization
    Jeffrey D. Bleil 253

    15. Section IV. Gene Expression: Messenger RNA

  15. Radioisotope Labeling of UTP and ATP Pools and Quantitative Analysis of RNA Synthesis
    Kerry B. Clegg and Lajos Pikó 283
  16. Quantitative Analysis of Specific Messenger RNAs by Hybridization Using RNA Probes
    Kent D. Taylor and Lajos Pikó 283
  17. Quantitative Analysis of Specific Messenger RNAs by Ribonuclease Protection
    Ross A. Kinloch, Richard J. Roller, and Paul M. Wassarman 294
  18. Analysis of Messenger RNA
    Mayi Y. Arcellana-Panlilio and Gilbert A. Schultz 303
  19. Quantitative Analysis of Specific Messenger RNAs in Small Numbers of Preimplantation Embryos
    Michelle F. Gaudette, Qiu Ping Cao, and William R. Crain 328
  20. Quantitative Analysis of Messenger RNA Levels: Reverse Transciption-Polymerase Chain Reaction Single Nucleotide Primer Extension Assay
    J. Singer-Sam and A.D. Riggs 344
  21. Microinjecting Antisense Sequences into Oocytes
    Fernando J. Sallés, William G. Richards, Joaquin Huarte, Jean-Dominique Vassalli, and Sidney Stickland 351
  22. Detection of Messenger RNA by in Situ Hybridizzation to Tissue Sections and Whole Mounts
    David G. Wilkinson and M. Angela Nieto 361
  23. Detection of Messenger RNA byin Situ Hybridization to Postimplantation Embryo Whole Mounts
    Ronald A. Conlon and Bernhard G. Herrmann 373
  24. Detection of Messenger RNA by in Situ Hybridization
    David Sassoon and Nadia Rosenthal 384
  25. Prepation of Injection Pipettes
    Miriam Miranda and Melvin L. DePamphilis 407
  26. Application of Firefly Luciferase to Preimplantation Development
    Miriam Miranda, Sadhan Majumder, Maria Wiekowski, and Melvin L. DePamphilis 412
  27. Application of LacZ Gene Fusions to Preimplantation Development
    Murial Vernet, Claire Bonnerot, Pascale Briand, and Jean François Nicolas 434
  28. Application of LacZ Gene Fusions to Postimplantation Development
    Claire Bonnerot and Jean-François Nicolas 451

    29. Section VI. Gene Expression: Proteins

  29. Two-Dimensional Gel Analysis of Protein Synthesis
    Keith E. Latham, James I. Garrels, and Davor Solter 473
  30. One Dimensional Gel Analysis of Histone Synthesis
    Maria Wiekowski and Melvin L. DePamphilis 489
  31. Whole-Mount Immunohistochemistry
    Claytus A. Davis 502
  32. Techniques for Localization of Specific Molecules in Oocytes and Embryos
    Calvin Simerly and Gerald Schattien 516

    33. Section VII. Gene Expression: Methylation

  33. Polymerase Chain Reaction for the Detection of Methylation of a Specific CpG Site in the Gópod Gene of Mouse Embryos
    Maurizio Zuccotti, Mark Grant, and Marilyn Monk 557
  34. Analysis of Methylation and Chromatin Structure
    G.P. Pfeifer, J. Singer-Sam, and A.D. Riggs 567

    35. Section VIII. Gene Identification

  35. Construction of Primary and Subtracted cDNA Libraries from Early Embryos
    Jay L. Rothstein, Dabney Johnson, Joel Jessee, Jacek Skowronski, Julie A. DeLoia, Davor Solter, and Barbara B. Knowles 587
  36. Consturction of cDNA Libraries from Single Cells
    Gerard Brady and Norman N. Iscove 611
  37. Construction and Characterization of Yeast Artifical Chromosome Libraries from the Mouse Genome
    Zoia Larin, Anthony P. Monaco, Sebastian Meier-Ewert, and Hans Lehrach 623
  38. Genome Developmentally Regulated Gene Families
    Brian J. Gavin and Andrew P. McMahon 653
  39. Cloning Developmentally Regulated Gene Families
    Brian J. Gavin and Andrew P. McMahon 653
  40. Screening for Novel Pattern Formation Genes Using Gene Trap Approaches
    David P. Hill and Wolfgang Wurst 664
  41. Insertional Mutagenesis by Retroviruses and Promotoer Traps in Embryonic Stem Cells
    Glenn Friedrich and Philippe Soriana 681
  42. Two-Dimensional Gel Analysis of Repetitve DNA Families
    Rachel D. Sheppard and Lee M. Silver 701

    43. Section IX. Nuclear Transplantation

  43. Transplantation of Nuclei to Oocytes and Embryos
    Keith E. Latham and Davor Solter 719
  44. Manipulations of Genetic Constitution by Nuclear Transplantation
    Sheila C. Barton and M. Azim Surani 732

    45. Section X. Transgenic Animals: Pronuclear Injection

  45. Production of Transgenic Mice
    Jon W. Gordon 747
  46. Factors Influencing Production Frequencey of Transgenic Mice
    Jeffrey R. Mann and Andre P. McMahon 771
  47. Surgical Techniques in Production of Transgenic Mice
    Jeffrey R. Mann 782
  48. Identification of Transgenic Mice
    Maureen Gendron-Maguire and Thomas Gridley 794

    49. Section XI. Transgenic Animals: Embryonic Stem Cells and Gene Targeting

  49. Derivation of Embryonic Stem Cell Lines
    Susan J. Abbondanzo, Inder Gadi, and Colin L. Stewart 803
  50. Production of Chimeras between Embryonic Stem Cells adn Embryos
    Colin L. Stewart 823
  51. Gene Targeting in Embryonic Stem Cells
    Ramiro Ramírez-Solis, Ann C. Davis, and Allan Bradley 855
  52. Simple Scerening Procedure to Detect Gene Targeting Events in Embryonic Stem Cells
    Frank Kõntogen and Colin L. Stewart 878
  53. Manipulation of Transgenes by Site-Specific Recombination: Use of Cre Recombinase
    Brian Sauer 890
  54. Embryonic Stem Cell Differentiation in Vitro
    Micheal V. Wiles 900
  55. Production of Tetraploid Embryos by Electrofusion
    K.J. McLaughlin 919

    56. Section XII. Lineage Analysis

  56. Lineage Analysis Using Retroviral Vectors
    Constance L. Cepko, Elizabeth F. Ryder, Christopher P. Austin, Christopher Walsh, and Donna M. Fekete 933

Author Index 961

Subject Index 991